BALLYA Sterigmatocystin Test is manufactured by BALLYA, it’s rapid test for detection sterigmatocystin residues in feed product, grain, edible oil, medicine and Chinese herbal medicin, especially in feed. Lateral flow assay test base on the gold immunochromatography assay technology.
Sterigmatocystin is mainly the final metabolite of Aspergillus Sterigmatocystin and Aspergillus nidulans, and it is also the synthesis of yellow blood by Aspergillus flavus and Aspergillus parasiticus Speare. An intermediate product in the late stage of the mycotoxin process.
Structure and characteristics of Sterigmatocystin
Sterigmatocystin is also a class of compounds with similar chemical structures. At present, there are more than ten kinds of determined structures. Except for heterochromatic aspergillus toxin, its chemical structure has two furan rings, which is similar in structure to aflatoxin. In addition to Aspergillus Sterigmatocystin, Aspergillus nidulans, Aspergillus pyogenes and Bipolaris solaginii can also produce aspergillus Sterigmatocystin.
What’s the Aspergillus Sterigmatocystin?
It is widely distributed in the air, soil, corrupt plant bodies, stored grains and various industrial and agricultural products. Aspergillus Sterigmatocystin is not only the main mildew during grain storage, but also a common fungus that causes mildew on various industrial equipment; in addition, it also produces a mycotoxin that can cause liver damage and cancer in animals: Sterigmatocystin
Morphology of Aspergillus Sterigmatocystin.
The growth of the colony is limited, the diameter is only 2～3 cm after 14 days of culture; the color change range is quite wide, such as velvety, flocculent or both. On the colonies of different strains, sometimes there will be light green, gray green and light yellow locally. Or pink and other colors, while the reverse side of the colony is almost colorless, yellow-orange or rose; some colonies will form colorless to purple-red droplets. The conidia heads are loose and radial, colorless or slightly yellow.
What are the hazards of Sterigmatocystin?
The pathological feature of acute sterigmatocystin poisoning is liver and kidney necrosis. However, the chronic toxic effect of sterigmatocystin is relatively strong, mainly manifested as the toxicity to the liver and kidney. In Ningxia, my country, equine animals and lambs have experienced symptoms of poisoning after eating feed contaminated with sterigmatocystin. Pathological necropsy is characterized by cirrhosis of the liver and high yellow staining of skin and internal organs.
Sterigmatocystin can also cause angiosarcoma, dorsal tissue hemangioma and other tumors in rats. Regarding the carcinogenic mechanism of Sterigmatocystin, some scholars believe that it is related to the double bond at the end of its difuran ring. This double bond can form an adduct with the uracil of the DNA molecule, causing the DNA structure to change and copy errors. At present, my country has not stipulated the limit standard of sterigmatocystin in food.
Sterigmatocystin toxicity
The oral LD50 of sterigmatocystin in rats is calculated in mg/kg body weight, 166 for males, 120 for females, and over 800 for mice. The LDso of monkeys was 32 by intraperitoneal injection. It can be seen from the LDso value that primates (monkeys) are more sensitive to sterigmatocystin than rodents. Sterigmatocystin toxin is carcinogenic and can also cause mutations in Bacillus subtilis and mouse cells.
How to control and prevent Sterigmatocystin?
The main measures taken at home and abroad to prevent and remove mycotoxin contamination in food are: ① Use reasonable farming, irrigation and fertilization, and timely harvest to reduce mold infection and toxin production of food raw materials; ② Take measures to reduce the water content of grain and feed, reduce storage temperature, improve storage, and add T to reduce mycotoxin pollution; ③Cultivate fungal-resistant crop varieties through resistance breeding; ④Strengthen pollution detection and inspection, strictly implement food hygiene standards, and prohibit the sale and import of food and feed that exceed mycotoxins; ⑤Remove contaminated mycotoxins by chemical and physical methods such as alkali refining method, activated white clay, attapulgite clay or aging soil and other chemical and physical methods
What specimen can be detected by BALLYA Sterigmatocystin Test?
Specimen include: all kinds of feeds, grain, edible oil, medicine and Chinese herbal medicine. Widely used in feed industry and food processing industry. You can choose the right products according to your needs.
Significance of tesing Sterigmatocystin
To protect the animal have health feed, grain, edible oil, medicine and Chinese herbal medicine and avoid contaminated food. The sterigmatocystin is harmful for animal being, can not take too much in the body.
1. Take feed specimen 2. Add the supernatant to cassette 3. Wait for 15 minutes to result 4. The details of operation, refer to the kit instruction
Limitations of BALLYA Sterigmatocystin Test?
BALLYA Sterigmatocystin Test is a qualitative test kit. It’s only for screening purpose. If have positive cases or suspected case, maybe use other detection method to make a further detection, such as, ELISA, PCR, qPCR, etc.
Conclusion
Sterigmatocystin is widespread in nature and poses a serious threat to people's daily food safety. Because of its structure is similar to aflatoxin, its toxicity and carcinogenicity are highly valued by countries all over the world. The BALLYA Sterigmatocystin Test provided by BALLYA can effectively detect the sterigmatocystin in the samples. Simple operation, short detection time and high accuracy are the characteristics of this product.
