Aflatoxin Rapid Test Kit is a rapid, one step test for the qualitative (or quantitative) detection of Aflatoxin in animal feed and grains (wheat, wheat bran, corns, coconut, millet, flour). The total assay time takes 8 min.
Aflatoxin B1 is the most carcinogenic chemical substance. Aflatoxin B1 has strong toxicity to humans and several animals, and its toxic effect is mainly to damage the liver.
Aflatoxin B1 is the most common and most harmful in natural food. The AQSIQ stipulates that aflatoxin B1 is one of the mandatory inspection items for most foods.
Generally speaking, aflatoxin b1 is found in many natural foods, such as corn, peanuts, peanut oil, beans, fermented foods, etc., so there are many food categories involved.
2. The characteristics of Aflatoxin B1
The pure product of aflatoxin B1 is colorless crystal with a relative molecular weight of 312.27. It is hardly soluble in water but easily soluble in oil and a variety of polar organic solvents, such as chloroform, methanol, ethanol, propanol, ethylene dimethyl amide, insoluble in petroleum ether, ether and hexane. Generally, it is stable in neutral solution, but decomposes slightly in strong acid solution, and decomposes rapidly in strong alkali solution with pH 9-10. Aflatoxin is relatively stable to light, heat, and can only be lysed when heated to 280-300 °C. After autoclaving for 2 hours, the toxicity will be reduced by 25%-33% and by 50% in 4 hours. Aflatoxin B1 fluoresces blue under ultraviolet light, and ultraviolet light is destructive to low concentrations of aflatoxin B1.
3. Aflatoxin B1 Pollution status
Aflatoxin B1 is the most carcinogenic chemical substance. Aflatoxin B1 has strong toxicity to humans and several animals, and its toxic effect is mainly to damage the liver.
Aflatoxin B1 is the most common and most harmful in natural food. The AQSIQ stipulates that aflatoxin B1 is one of the mandatory inspection items for most foods.
Generally speaking, aflatoxin b1 is found in many natural foods, such as corn, peanuts, peanut oil, beans, fermented foods, etc., so there are many food categories involved.
The hazards of Aflatoxin B1
The absorption and excretion of aflatoxin in the body are faster. If it is not a continuous intake, when the intake of a relatively small amount of food containing AFB1, after about 10 days, it will be excreted by various excretory systems. If the intake is too large, it will cause AFB1 acute poisoning and liver damage. AFB1 is currently known to have three strong substances: mutagenicity, carcinogenicity, and teratogenicity.
AFB1 mainly harms the liver and kidneys of humans and animals. Symptoms of poisoning mainly reflect various symptoms of hepatocellular disease, liver necrosis, and liver cancer. It is absorbed and transformed in various gastrointestinal systems in the body, and transformed into various homologues of AFB1, such as AFM1, AFM2, AFP1, AFQ, etc., through various enzymatic demethylation, epoxidation, and hydroxylation reactions. These transformed substances can bind to various proteins or enzymes in the body, affect or change the properties of eggs, interfere with normal protein mechanisms, and cause cell cancer.
The toxicity of aflatoxin B1 is 30 times toxic than vomitoxin, and 20 times toxic than zearalenone. The acute toxicity of aflatoxin B1 is 10 times that of potassium cyanide and 68 times that of arsenic. The chronic toxicity can induce cancer. The carcinogenic ability is 75 times that of dimethylnitrosamine and 900 times that of dimethylaminobenzene. Human primary liver cancer may also be related to aflatoxin.
When people ingest large amounts of aflatoxin B1, acute poisoning can occur, including acute hepatitis, hemorrhagic necrosis, hepatocyte steatosis and bile duct hyperplasia. Continuous intake of a small amount can cause chronic poisoning, growth failure, fibrous lesions, and proliferation of fibrous tissue.
Poultry: Reduced feed intake, body weight gain and feed conversion rate; infertility, miscarriage; pulmonary edema, increased susceptibility to disease. Pigs: Reduced feed intake, body weight gain and feed conversion rate; infertility abortion; pulmonary edema, increased susceptibility to disease.
Features of Aflatoxin B1 Test
1. 8min reading results
2. Detection limits respond to EU and US FDA norms
3. Single test for every target sample
4. Neither professionals nor equipment required
5. Eyes interpretation or quantitative measurement, suitable for lab and field use
6. Room temperature storage
7. Non-toxic and harmless
Detection method of Aflatoxin B1 test
The Aflatoxin B1 Rapid Test Kit is a colloidal gold immunochromatography assay that detects aflatoxin B1 residue in grain, feed and meets EU MRL. BALLYA colloidal gold detection kit can meet the rapid requirements of the field, and result within 5-10 minutes, and can be qualitative or quantitative with high sensitivity.
This product uses the colloidal gold competition method to quickly detect the residues of aflatoxin B1 in cereals and feeds through specific antigen-antibody reactions. It is suitable for on-site rapid detection of various enterprises, testing institutions and supervision departments.
Samples for Aflatoxin B1 test
Crops that are susceptible to aflatoxin B1 contamination include Corn, cottonseed, millet, wheat, peanuts, sorghum, DDGS, barley, rye, rice, soybeans, sunflower seeds, nuts and oats.
Limit of detection for Aflatoxin B1 test
The LOD may change according to requirement or specimen's difference, please refer to kit instruction for further details.
The Importance of Aflatoxin B1 test
Aflatoxin B1 test can help manufacturers, regulatory agencies, and the public monitor product quality and help humans protect their health.
Component of Aflatoxin B1 test
1. Micro-Well Reagent: 8 tests/tube, 12 tubes/box
2. Test Strip: 8pcs/tube, 96pcs/box
3. Manual Instruction: 1pc
Materials Required for Aflatoxin B1 Test
1. Micropipette 10-200ul
2. Tips for mini pipette, 96pcs
3. Microwells
4. Timer
How to use Aflatoxin B1 Test?
1. Add 200ul test sample into micro well reagent.
2. Incubate for 2 minutes.
3. After 8 minutes, read the results.
4. Refer to the kit instruction for further details.
Interpretation of Aflatoxin B1 Test
Conclusion
Aflatoxin B1 is widely present in nature and pollutes our food, animals feed, oil… almost all the resources on which we depend for survival. Therefore, timely detection of the presence of Aflatoxin B1 is particularly important.
The aflatoxin B1 test provided by BALLYA can remove your doubts by timely and accurately detecting whether food and beverage contain aflatoxin B1.
How to order Aflatoxin B1 Test?
Please check the online catalog and contact our sale representative via email: info@ballyabio.com or fill out contact form below:
