One Step BT Sensor

Betalactam+Tetracycline Combo Test Kit
Beta lactamase Tetracyclines Test Kit

One Step BT Sensor

For Milk, Milk Powder, Pasteurized Milk

Specs:96T / box
Testing Time:7-10 mins
Regulation: CN / EU / RU / US

One Step BT Sensor 
Only insert the strip into the milk sample, just waiting 7 minutes to get reliable results

The operation guideline for ONE STEP BT Sensor download

What is beta lactam tetracycline combo test?

Two-Sensor Kit, rapid to detect two types antibiotics residues in milk and dairy product by using colloidal gold immune-chromatography technology. As soon as 7 minutes to results.
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What are beta lactams?

1. Definition

Beta-lactam antibiotics are a wide range of antibiotics, including penicillin and its derivatives, cephalosporins, monoamide rings, carbapenems and penicillene enzyme inhibitors etc. β-lactam antibiotics (beta-lactams) refer to a large class of antibiotics with β-lactam ring in the chemical structure. 

Basically all antibiotics that include β-lactam core in their molecular structure belong to β-lactams Antibiotics, it is the most widely used class of existing antibiotics, including penicillins and cephalosporins that are most commonly used clinically, as well as newly developed cephalosporins, thiomycins, monocyclic β-lactams, etc.

Such antibiotics have the advantages of strong bactericidal activity, low toxicity, wide indications and good clinical efficacy. The chemical structure of this class of medicines, especially the change of the side chain, has formed many antibiotics with different antibacterial spectrum and antibacterial effects and various clinical pharmacological properties.

2. Mechanism of action

The mechanism of action of various beta lactam antibiotics is similar. They can inhibit cell wall mucopeptide synthase, namely penicillin binding proteins (PBPs), thereby hindering the synthesis of cell wall mucopeptides, causing bacterial cell wall defects and bacteria Body expansion and cracking. 

In addition, the lethal effect on bacteria should also include triggering the autolysin activity of the bacteria, and resistance of mutant strains lacking autolysin. Animals have no cell wall and are not affected by β-lactam drugs. Therefore, this class of drugs has a selective bactericidal effect on bacteria and has low toxicity to the host.

What is tetracycline?

1. Definition

Tetracyclines are a class of broad-spectrum antibiotics produced by actinomycetes, including chlortetracycline, oxytetracycline, tetracycline and semi-synthetic derivatives of methenoxytetracycline, powerful Mycin, dimethylaminotetracycline, etc., their structures all contain the naphthacene skeleton.

Tetracycline antibiotics are unstable under acidic and alkaline conditions. Tetracycline drugs contain many hydroxyl groups, enol hydroxyl groups and carbonyl groups, and can form insoluble chelates with a variety of metal ions under neutral conditions. It forms insoluble calcium or magnesium salts with calcium or magnesium ions, forms a red complex with iron ions, and forms a yellow complex with aluminum ions. In the body, the complex formed by the tetracyclines drug and calcium ions is deposited on the bones and teeth in a yellow color. 

The teeth will turn yellow when taken by children. After pregnant women take it, their babies may have tooth discoloration and inhibited bone growth. Therefore, children and pregnant women should use this medicine with caution or refrain from taking it.

2. Mechanism of action

This product is a broad-spectrum antibacterial agent, which has a bactericidal effect at high concentrations. In addition to common gram-positive bacteria, gram-negative bacteria and anaerobic bacteria, most of the Rickettsia, Mycoplasma, Chlamydia, Atypical Mycobacterium, and Spirochetes are also sensitive to this product. 

The effect of this product on Gram-positive bacteria is better than Gram-negative bacteria, but Enterococcus is resistant to it. Others such as Actinomyces, Bacillus anthracis, Listeria monocytogenes, Clostridium, Nocardia, etc. are sensitive to this product. This product has certain antibacterial activity against Neisseria gonorrhoeae, but penicillin-resistant Neisseria gonorrhoeae is also resistant to tetracycline.

The harm of beta lactam and tetracycline

Tetracycline antibiotics include chlortetracycline, oxytetracycline and tetracycline, etc., which can be used as veterinary drugs to prevent and treat livestock diseases or as feed additives to promote the growth of livestock and poultry, and are widely used in edible animals.

However, excessive use inevitably forms the metabolites and other related antibiotics remains in the animal's muscles, eggs, milk, and organ tissues affecting human health through the food chain. The abuse of antibiotics causes bacteria to develop resistance, bringing difficulties to the treatment of human diseases.

Features of beta lactam tetracycline combo test

1. 7min reading results

2. Detection limits respond to EU and US FDA norms

3. Neither professionals nor equipment required

4. Easy to operate

Detection Method of Beta lactam tetracycline combo test

The Beta lactams Tetracyclines Combo Test is a colloidal gold immunochromatography assay that detects Penicillin G, Penicillin V, Ampicillin, Amoxicillin, Oxacillin, Cloxacillin, Dicloxacillin, Nafcillin, Cefquinome, Cefacetrile, Cefalonium, Cefazolin, Cefoperazone, Cephapirin, Ceftiofur and Tetracycline in milk, rawcycline, Chlorine, Oxacillin, milk pasteurized milk and meets EU MRL.

Samples for beta lactam tetracycline combo test

Samples include: raw milk, milk powder, pasteurized milk.

Limit of Detection for beta lactam tetracycline combo test

The LOD may change according to requirement or specimen's difference, please refer to kit instruction for further details.

Importance of beta lactam tetracycline combo test

Beta lactam tetracycline combo test can help manufacturers, regulatory agencies, and the public monitor product quality and help humans protect their health.

Components of Beta lactam tetracycline combo test

1. Micro-Well Reagent: 8 tests/tube, 12 tubes/box

2. Test Strip: 8pcs/tube, 96pcs/box

3. Manual Instruction: 1pc

Materials of Beta lactam tetracycline combo test

1. Sample Tube

2. Pipette & Pipette Tip

3. Well-Tray-Plate

How to use beta lactam tetracycline combo test?

1. Add 200ul milk into a well, then pipet up and down for 10 times to completely mix the sample with the reagent in the well.

2. Then incubate for 5min.

3. Insert the test strip into the micro-well with the sample pad fully dipped.

4. After 5 minutes, read the results.


When antibiotics were first used on humans, we regarded them as "magic drugs". As the problem of antibiotic abuse continues to worsen, their resistance has also brought huge harm to humans.

Therefore, in daily life, we should avoid food with antibiotic residues. The first step to self-help: we need to detect antibiotic residues.

How to order beta lactam tetracycline combo test?

Please check the online catalog and contact our sale representative via email: or fill out contact form below:

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