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Point Of Care: How To Develop Lateral Flow Assay Rapid Test?

Posted on: June 4, 2020, by ballyabio

How to develop rapid test (POCT)?
(Only for research and reference!)


How To Develop Lateral Flow Rapid Test

Antibodies & Antigen Preparation

For example, if develop COVID 19 Antibody Test, then
1. 1mg SARS-CoV-2 Spike Protein
2. 1mg Goat anti-Human IgG (Secondary Antibody, for IgG line)
3. 1mg Goat anti-Human IgM (Secondary Antibody, for IgM line)
4. 2mg Goat Anti Mouse IgG (For Control Line)


IVD Supplies

1. PVC BACKING CARD for lateral flow assay
Dimension 300mm x 60mm, thickness 0.3mm

conjugate-pad-and-absorbent-pad-backing-card
conjugate-pad-and-absorbent-pad-backing-card

2. Sample Pad for lateral flow assay
Select: SC-75

sample-pad-for-lateral-flow-assay
sample-pad-for-lateral-flow-assay

3. Conjugate Pad for lateral flow assay
Select: SC-75 (Can be used as sample pad and conjugate pad.)

4. Nitrocellulose Membrane

nitrocellulose membrane NC140
nitrocellulose membrane NC140

5. Blood Separation Mambranes

6. Absorbent Pad
Select: AP-F270

7. Cassette Case

Cassette Case For IVD
Cassette Case For IVD

8. Desiccant

9. Foil Pounch

Foil Pouch For IVD
Foil Pouch For IVD

10. Gold Nanoparticles

gold-nanoparticles-ivd
gold-nanoparticles-ivd

Lab Equipment

1. Automatic Dispenser Machine (dispense colloid gold, antibodies, antigens in one platform)

BIODOT Low Volume Precision Dispensing Equipment
BIODOT Low Volume Precision Dispensing Equipment

2. Strip Cutter

lateral-flow-assay-strip-cutter
lateral-flow-assay-strip-cutter

3. Cassette Case Processor

Cassette Case Processor
Cassette Case Processor

4. Sealing Machine

Sealing Machine
Sealing Machine

5. Laboratory Oven

Laboratory Oven
Laboratory Oven

In Vitro Diagnostics: Manufacturing Flow Chart

1. Colloidal Gold Preparation

How to make colloidal gold?
How to make colloidal gold?

2. Antibody Nanopaticle Conjugation

Colloidal Gold Labeling
Colloidal Gold Labeling

3. Gold Nanopaticle Dispensing
It can be dispensed by manual or by automated machine.

Dispensing Colloidal Gold Manual
Dispensing Colloidal Gold Manual
Colloidal Gold Dispensing By Automated Machine
Colloidal Gold Dispensing By Automated Machine

4. Nitrocellulose Membrane Dispensing

nitrocellulose membrane dispensing
nitrocellulose membrane dispensing

5. Drying Procedure By Oven

Lab Oven
Lab Oven Drying

6. Lamination

Lamination Procedure
Lamination Procedure

7. Uncut Sheet Format

Uncut Sheet IVD lateral flow
Uncut Sheet IVD Lateral Flow

8. Strip Cutting

Strips Cutting Procedure IVD
Strips Cutting Procedure IVD

9. Cassette Assembling

Cassette Case Assembling
Cassette Case Assembling

10. Kit Assembling

Rapid Test Assembling
Rapid Test Assembling

11. Sealing In Foil Punch

Sealing In Foil Pouch lateral flow
Sealing In Foil Pouch lateral flow
immunoassay
Immunoassay
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