The commonly used methods for mycotoxin detection
are as follows:
The TLC method is aimed at different samples. The mycotoxin is extracted from the sample with a suitable extraction solvent, purified by column chromatography, and then expanded and separated on a thin layer chromatography. The fluorescence of mycotoxins is used to determine the minimum content by comparing its strength with the standard. TLC sample preparation is cumbersome, and the extraction and purification effects are not ideal. There are many impurities in the extraction solution, which affects the fluorescence intensity of the spots during expansion.
Chromatography, including thin-layer chromatography, gas chromatography, and liquid chromatography, has always been the most important chemical analysis method for mycotoxins. The more common method for analyzing mycotoxins is still liquid chromatography, including liquid chromatography-mass spectrometry. This method is fast and accurate, but requires expensive instruments and equipment. It is limited to professional testing institutions' access to scientific research, investigation, analysis, and monitoring. It has not been widely used in enterprises and the grassroots. Moreover, the lag effect of its results has greatly reduced the actual production Guidance effect.
3. Immunochemical detection method: (colloidal gold immunochromatography, enzyme-linked immunosorbent assay):
Immunological detection method is a biochemical analysis method based on the selective reaction between antibodies and antigens or haptens. Usually has high selectivity and low detection limit. It is widely used for the determination of various antigens, semi-antibodies or antibodies. Generally, it can be divided into fluorescent immunoassay.
The best mycotoxin test medium is urine, as mycotoxins accumulate in the liver and kidneys. So testing urine is also easier and less invasive.