BALLYA ochratoxin test for beer and wine is a quick one-step test that can qualitatively (or quantitatively) detect ochratoxin in beer and wine in just eight minutes.
Ochratoxin is a mycotoxin drawing worldwide attention. It is a group of important food-contaminating mycotoxins produced by 7 species of Aspergillus of the genus Aspergillus and 6 species of Penicillium of the genus Penicillium. Four of them are the most toxic, the most widely distributed with the highest toxin production, and the most harmful to agricultural products. The most polluted and most closely related to human health is ochratoxin A
The toxicity of ochratoxin to animals and humans mainly includes kidney toxicity, liver toxicity, teratogenicity, carcinogenicity, mutagenic and immunosuppressive effects. After entering the body, ochratoxin A is converted into 4-hydroxy ochratoxin A and 8-hydroxy ochratoxin A under the action of liver microsomal mixed function oxidase, of which 4-hydroxy ochratoxin A is the main component. The order of toxicity of ochratoxin is: OTA>OTC>OTB.
can produce a toxic concentration of ochratoxin at a low temperature of 4 °C. Ingesting 1ppm body weight dose of ochratoxin A can be lethal to animals within 5-6 days. Common lesions are renal tubular epithelial injury and necrosis of intestinal lymph glands. Feeding a diet containing 1ppm ochratoxin for 3 months can cause polydipsia, frequent urination, growth retardation and reduced feed utilization.
Ochratoxin blocks the action of amino acid tRNA synthetase and affects protein synthesis, reducing IgA, IgG and IgM and reducing antibody titer. It can damage poultry Bursa of Fabricius and intestinal lymph tissue of livestock and poultry, reduce antibody production, and affect humoral immunity, which is related to the carcinogenic effect of ochratoxin. It can also cause granulocyte phagocytic ability to decrease, thereby affecting phagocytosis and cellular immunity.
The molds that produce ochratoxin are widely distributed in nature, resulting in ochratoxin A being widely distributed in various foods and feeds
. OTA has residues in monogastric animal tissues and corresponding animal products. OTA in food is related to a fatal endemic kidney disease (balkanendemicnephropathy) and has carcinogenic and teratogenic effects. In recent years, it has caught the eyes of human nutritionists.
If pigs eat ochratoxin-contaminated feed for a long time, mycotoxins
can contaminate most of the edible tissues of pigs, leading to kidney damage and lowering of pork carcass. Acute ochratoxin poisoning (diet toxin levels higher than 5ppm) is characterized by nephropathy (kidney failure), enteritis fatty liver, lymph node necrosis, immunosuppression, and other pathological symptoms.
Due to acute renal failure, acute ochratoxin poisoning may cause animal death. In view of the characteristics of ochratoxin that can accumulate in the edible muscle tissue of animals and cause human health problems, researchers have recently focused their research on the carcinogenicity of ochratoxin. In fact, the Danish swine industry uses kidney ochratoxin levels as an indicator to determine whether there are potential toxin residues in pork products. Clinical symptoms and necropsy can show ochratoxin poisoning, which can also be confirmed by monitoring mycotoxins in feed or detecting toxin levels in the kidneys at the slaughterhouse.
Since the half-life of ochratoxin in serum is quite long (72-120 hours), pigs are very sensitive to ochratoxin contamination. Researchers have recently tested and investigated the natural pollutant ochratoxin in pig blood in Canada and the European Union, including Germany, Norway, Poland, Sweden and the former Yugoslavia. At the same time, surveys in the United States, Austria, Belgium, Denmark, Finland, Germany, Poland, Switzerland, the United Kingdom and the former Yugoslavia showed that ochratoxin also appeared in pig kidneys.
Ochratoxin residues in animal products can be passed to consumers through the food chain. Governments in some countries have adopted tough regulatory measures to eliminate consumers' concerns about the safety of pork products. For example, in 1997, Europe established the maximum allowable content of ochratoxin in all foods as 5ppb. Germany set this standard more strictly as 3ppb. In Denmark, if the blood ochratoxin level of pigs reaches 25μg/mL, it is considered that the entire carcass of the pig is contaminated and pork cannot be used for food.
The Ochratoxin Rapid Test Kit is a colloidal gold immunochromatography assay
that detects Ochratoxin residue in grain, feed and meets EU MRL.
This product uses the colloidal gold competition method to quickly detect the residues of ochratoxin in cereals and feeds through specific antigen-antibody reactions. It is suitable for on-site rapid detection of various enterprises, testing institutions and supervision departments.
Compared with instrumental analysis technology, this kit can economically and quickly detect ochratoxin in samples of grain
, feed, flour, beer, red wine, and beverages.
Ochratoxin testing can help manufacturers strictly control product quality and help humans protect their health.
1. Add 200ul test sample into micro well reagent.
2. Incubate for 2 minutes.
3. After 8 minutes, read the results.
4. Refer to the kit instruction for further details.
Ochratoxin is widely present in nature, polluting our food, feed, beer and all the resources on which we depend for survival. It is particularly important to detect the presence of ochratoxin in time.
The ochratoxin test provided by BALLYA
can help you solve problems, timely and accurately detecting whether food and beverage contain ochratoxin.
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