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beta-lactam Test

beta-lactams-Test-Kit

For Milk, Milk Powder, Pasteurized Milk

Specs:96T / box
Testing Time:7-10 mins
Regulation: CN / EU / RU / US

Operation video

Instrution For beta-lactam Test

Introduction
Single Test Kit, rapid to detect an antibiotic residues in milk and dairy product by using colloidal gold immunochromatography technology. As soon as 7-10 minutes to results.

Application
For raw milk, whole milk powder, Pasteurized milk.

Spicifications
1. Specificity
Not cross-react with macrolides, aminoglycosides, sulfonamides drugs, etc.

2. Limit of Detection
Refer to instruction.

Storage & Shelf-Life
At 2~8℃ out of sunlight, 12 months

Compolents (96T / box)
Instruction                          1
Tube                                     12

Equipments (Option)
Timer
Incubator
Mini Pipette(200μL)
Tips for mini pipette
Reader

Operations
1. Take 200 μL milk specimen, add into micro well, mix with the pink agent 10 times.
Remark: avoid specimen residues too much in the well.
2. Incubate 3 minutes
3. Insert strips into the wells
4. Incubate 5 minutes
5. Take out strips and read results by eye or reader.

Interpretation
Visual inspection
1. If C line visible, interpret according to the instruction. T line compare with C line to interpret positive or negative.
2. If C & T are invisible, it’s invalid detection, repeat testing again.

Reader Interpretation
Refer to the instruction

Results Illustration

If you want to know more, please contact us

Trivia questions: What is beta-lactam?

Beta lactam definition

Beta lactam

Cyclic amides, the Greek letter used to indicate the number of rings: β-lactam (four-membered ring).

Beta lactam structure

Beta lactam have the advantages of strong bactericidal activity, low toxicity, wide indications and good clinical efficacy. The chemical structure of this class of drugs, especially the side chain changes, has formed many antibiotics with different antibacterial spectrum and antibacterial effect, as well as various clinical pharmacological properties.

Beta lactam ring

The beta-lactam ring is part of the core structure of several antibiotic families. The main families are penicillin, cephalosporins, carbapenems, and monobactams, so it is also called beta-lactam antibiotics. Almost all of these antibiotics work by inhibiting the biosynthesis of bacterial cell walls. This has a lethal effect on bacteria, although any given bacterial population will usually contain a subgroup that is resistant to beta-lactam antibiotics. The development of bacterial resistance is due to the expression of many genes.

Beta lactamase

Beta lactamase is a type of enzyme secreted by drug-resistant bacteria against lactam antibiotics. It can bind to the β-lactam ring, cleavate the β-lactam ring and be destroyed, and lose antibacterial activity. Is the main reason for this type of bacteria resistance.

Beta lactam antibiotics

Beta lactam antibiotics list

Penicillins
Penicillins can be divided into narrow-spectrum penicillin, enzyme-resistant penicillin, broad-spectrum penicillin, anti-Pseudomonas aeruginosa broad-spectrum penicillin, and anti-gram-negative bacteria penicillin.

Cephalosporins
Can be divided into first-generation cephalosporins, second-generation cephalosporins, third-generation cephalosporins, and fourth-generation cephalosporins.

Other β-lactams
Including carbapenems, cephalomycins, oxycephems, and monocyclic β-lactams

How does beta lactam work?

Beta lactam mechanism of action

The mechanisms of action of various β-lactam antibiotics are similar. They can inhibit cell wall mucin synthetase, penicillin binding proteins (PBPs), thereby hindering cell wall mucin synthesis and causing bacterial cell wall defects Body swelling and lysis. In addition, the lethal effect on bacteria should include triggering the bacteria's autolysin activity, and mutant strains lacking autolysin showed resistance.

Beta lactam drugs

Beta lactam penicillin

Penicillin G is the earliest antibiotic used in clinical practice. Because of its advantages such as strong bactericidal power, low toxicity, low price, convenient use, etc., it is still the drug of choice for treating various infections caused by sensitive bacteria. However, penicillin has disadvantages such as intolerance to acid, penicillinase, narrow antibacterial spectrum, and easy to cause allergic reactions, and is limited in clinical application.

Beta lactamase inhibitor

β-lactamase inhibitors are a new class of β-lactam drugs. Plasmid delivery produces β-lactamase, which causes hydrolysis of some drugs' β-lactam ring and inactivation. It is the main way that pathogens are resistant to some common β-lactam antibiotics (penicillins and cephalosporins).

Beta lactam side effects

Side effects of β-lactam antibiotics include: diarrhea, dizziness, rashes, urticaria, overlapping infections (including Candida) (Rossi, 2004). Occasionally β-lactam antibiotics can cause fever, vomiting, erythema, skin Inflammation, angioedema and pseudomembranous enteritis.
Beta-lactam antibiotics and beta-lactamase inhibitors are often painful and inflamed when injected at the same time.

Beta lactam allergy

About 10% of patients are allergic to beta-lactam antibiotics. Allergic reactions occur in about 0.01% of patients (Rossi, 2004). About 5-10% of patients are cross-sensitive to penicillin derivatives, cephalosporins, and carbapenem antibiotics.

Summary

Β-lactam antibiotics represented by penicillin and cephalosporins are characterized by high efficiency, high safety, and broad-spectrum antibacterial. They occupy an extremely important clinical position in clinical practice. Today it remains the antibiotic of choice for G + cocci infections. β-lactam antibiotics can also be used as veterinary antibiotics in the dairy farming industry, so the milk produced may have residual β-lactam antibiotics. Ballya has specifically studied this situation and developed a rapid test kit for β-lactam antibiotics. Yes, people in need can quickly and easily detect β-lactam antibiotics in milk. The test results comply with EU standards. Not only is the operation simple, but the time is short and the accuracy is high. Ballya will continue to do more research in this area.

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