Beta-lactam antibiotics are antibiotics containing four atoms in the molecule to form a lactam ring. β-lactam antibiotics form a covalent bond through the penicillin binding protein related to the synthesis of bacterial cell wall, destroy the synthesis of bacterial cell wall, inhibit the growth of bacteria with excellent selectivity. They are the most important class of anti-infective drugs.
After half a century of development, it has expanded from penicillane derivatives to oxypenem, penicillene, oxypenem, carbapenem, cephem, oxycephalosporin, carbacephem and monocyclic β- Lactams and so on.
β-lactam antibiotics (β-lactams) refer to a large class of antibiotics with β-lactam ring in the chemical structure. Basically all antibiotics that include β-lactam core in their molecular structure belong to β-lactams Antibiotics.
It is the most widely used class of existing antibiotics, including penicillins and cephalosporins that are most commonly used clinically, as well as newly developed cephalosporins, thiomycins, monocyclic β-lactams, and Atypical β-lactam antibiotics. Such antibiotics have the advantages of strong bactericidal activity, low toxicity, wide indications and good clinical efficacy.
The chemical structure of this class of medicines, especially the changes in the side chains, has formed many antibiotics with different antibacterial spectrum and antibacterial effects and various clinical pharmacological properties.
3. Mechanism of action
The mechanism of action of various β-lactam antibiotics is similar, and they can inhibit cell wall mucopeptide synthase, namely penicillin binding proteins (PBPs), thereby hindering the synthesis of cell wall mucopeptides, causing bacterial cell wall defects and bacteria Body expansion and cracking.
In addition, the lethal effect on bacteria should also include triggering the autolysin activity of the bacteria, and mutant strains lacking autolysin show resistance. Animals have no cell wall and are not affected by β-lactam drugs. Therefore, this class of drugs has a selective bactericidal effect on bacteria and has low toxicity to the host.
The harm of Betalactam
β-Lactamase (Magnolia enzyme preparation), which is produced and secreted by Gram-positive bacteria, can selectively decompose residual β-lactam antibiotics in milk. β-lactamase is a food additive that is not allowed in China. The use of this enzyme will cover up the antibiotics actually contained in milk.
β-lactamase may be illegally used as a decomposer of antibiotics in milk. It is artificially added to cow's milk to degrade the residual antibiotics in cow's milk and turn milk with excessive antibiotic residues into "qualified" milk. β-lactamase can destroy the structure of penicillin lactam and lose its activity. Long-term human intake may lead to increased resistance to antibiotics such as penicillin and cephalosporin, which greatly reduces the body’s ability to resist infectious diseases.
In February 2009, the illegally added inedible substances that mask antibiotics in dairy products were included in the “List of inedible Substances (Second Batch) that Maybe Unlawfully Added to Foods” published by the National Leading Group for the Special Rectification of Combating the Illegal Addition of inedible Substances and Abuse of Food Additives by the Ministry of Health and other nine departments.
Beta-lactam antibiotics are often used in the animal husbandry to prevent and control animal infectious diseases. Due to improper use or non-compliance with the withdrawal period regulations, the residues of this type of antibiotics in animal-derived foods exceed the standard, causing serious harm to human health.
On one hand, humans often drink milk with antibiotic residues, which is equivalent to long-term low-dose antibiotics. The pathogenic bacteria in the human body will develop resistance to many antibiotics. It can also cause the production of drug-resistant strains of pathogens in the human body, increase the difficulty of disease treatment, and can also lead to the occurrence of adverse symptoms such as allergies, poisoning and teratogenicity. In addition, the artificial addition of B-lactamase may also introduce a variety of harmful substances, which may cause adverse reactions such as rash, fever or anaphylactic shock.
On the other hand, the use of β-lactamase masks the residual problems caused by antibiotic abuse. At the same time, it also promotes the abuse of antibiotics in the process of animal husbandry, resulting in a certain vicious circle, and further increases the resistance of bacteria to anti-cables, resulting in the widespread emergence of super bacteria, and eventually faces the situation of no medicines available.
Features of Betalactam Test
1. 7min reading results
2. Detection limits respond to EU and US FDA norms
3. Neither professionals nor equipment required
4. Easy to operate and read
Detection Method of Betalactam Test
The Beta-Lactams Test Kit is a colloidal gold immunochromatography assay that detects Penicillin G, Ampicillin, Amoxicillin, Oxacillin, Cloxacillin, Dicloxacillin, Nafcillin, Cefquinome, Cefacetrile, Cefalonium, Cefazolin, Cefoperazone, Cephapirin, Ceftiofur in pasteurized milk, raw milk, milk and meets EU MRL. This kit can be applied for on-site rapid testing by various departments.
Samples of Betalactam Test
Samples include: raw milk, milk powder, pasteurized milk.
Detection limit of Betalactam Test
The LOD may change according to requirement or specimen's difference, please refer to kit instruction for further details.
Importance of Betalactam Test
Betalactam Test can help manufacturers, regulatory agencies, and the general public monitor product quality and help humans protect their health.