What is BALLYA Gastroenteritis Test?
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is manufactured by BALLYA, it’s rapid test for detection Gastroenteritis disease in pigs. Lateral flow assay test base on the gold immunochromatography
What is Transmissible gastroenteritis?
Infectious gastroenteritis refers to infectious acute gastroenteritis. The pathogens of infection may be viruses, bacteria or parasites. The clinical manifestations are mainly digestive symptoms such as nausea, vomiting and diarrhea. The cause of infectious gastroenteritis is a virus or a bacterium. Further examination of the pathogen is required, and the cause is not good according to the symptoms.
What is the Transmissible gastroenteritis virus?
Porcine infectious gastroenteritis virus (TGEV
) belongs to the Coronavirus family and members of the Coronavirus genus. The virus particles are polymorphic, most of which are round or oval, with a diameter of 60 ~ 160 nm. There are petal-shaped fibroids, and the virus particles are divided into 3 structural levels, which are the surface double-layer capsule, the internal core structure, and the nucleocapsid released by the degradation of the core structure. Three types of proteins are interspersed in the lipid bilayer: membrane protein (M protein), fibrillin (S protein), and vesicle membrane protein (sM protein). Inside is the nucleocapsid protein (N protein).
Transmissible gastroenteritis virus genome
The TGEV genome is a non-segmented, single-stranded positive-stranded RNA with a cap structure at the 5 'end and a poly (A) tail at the 3' end. The gene and the nuclear egg are self-associated without segmentation and infectious. The entire genome is approximately 2.85 × 104bp in length and has a molecular mass of 6 × 103ku. It is divided into 7 regions, each region has one or two open reading frames (ORFs), and there are conserved sequences between the subgenomic mRNAs to separate them. The structure is 5'-ORFla + ORFlb-S-ORF3a-ORF3b-sM-MN-ORF7-3 '.
Transmissible gastroenteritis virus pathology
TGEV invades the body through the digestive and respiratory tracts. The virus enters the digestive tract through the oropharynx, esophagus, and stomach, and infects the epithelial cells of the small intestine, causing the villi of the jejunum and ileum to shrink significantly. Infected epithelial cells are damaged and shed, which destroys the small intestinal epithelial cells' absorption function, reduces the intestinal hydrolysis of lactose and the absorption of other nutrients, the hypertonicity in the intestinal cavity, resulting in severe diarrheal dehydration. TGE virus enters the respiratory tract through the nasal cavity, multiplies in the nasal mucosa and lungs, and then enters the small intestine through the blood.
Transmissible gastroenteritis of swine
Porcine infectious gastroenteritis is a highly contactable digestive tract infectious disease of pigs caused by porcine infectious gastroenteritis virus. It is characterized by vomiting, watery diarrhea, and dehydration. OIE lists it as a Class B animal disease.
Transmissible gastroenteritis of swine symptom
Generally, piglets within 2 weeks of age will vomit 12-24 hours after infection, followed by severe watery or pasty diarrhea, yellow stools, often with undigested curd lumps, foul odor, and rapid weight loss. Dehydration, death 2-7 days after onset, mortality rate reaches 100%; in piglets 2-3 weeks old, mortality rate is 0-10%. Onset of weaning pigs occurred 2-4 days after infection, showing watery diarrhea, spray-like, gray or brown stool, individual pigs vomiting, diarrhea stopped after 5-8 days, rarely died, but weight loss, often showing stunted growth, become a zombie. Some sows have repeated infections in close contact with diseased piglets, with severe symptoms, elevated body temperature, lactation cessation, vomiting, loss of appetite, and diarrhea, and some nursing sows do not show clinical symptoms.
Transmissible gastroenteritis of swine diagnosis
A preliminary diagnosis can be made according to the season, age, and clinical characteristics of the disease, and laboratory tests must be performed to confirm the diagnosis. This disease should be distinguished from epidemic diarrhea of pigs, rotavirus infection, E.coli disease and swine dysentery, piglet paratyphoid, and piglet hypoglycemia.
How to treat Transmissible gastroenteritis?
The following drugs can be used to control secondary infections: first, atropine is injected first, at a dose of 2-4 mg per head; severely ill pigs can be closed after the acupoint. Finally, Changduqing (Guohao) was 100 kg / set for 2-3 days, and 2-6 g of bismuth hyponitrate or 2-4 mg of tannic acid and 2-5 g of activated carbon were taken orally. Chinese medicine can be selected from charcoal, cinnamon, calcined keel, Shenqu, decoction or infusion. The effect is excellent twice a day.
Transmissible gastroenteritis prevention
① Vaccination. For pregnant sows, about 45 days and 15 days before delivery, vaccination with post-sea acupoints with attenuated vaccines will give the sows a certain degree of immunity, so that lactating piglets after birth can obtain passive immunity protection from maternal antibodies.
② Strengthen breeding management and create good environmental conditions, especially in the cold season between late autumn and early spring. The enclosure should maintain a certain temperature, reasonable light and suitable density. Feed the full price of feed to increase the body's resistance.
③ Insist on regular disinfection, thoroughly remove feces and turf, and use 2% to 3% sodium hydroxide to comprehensively disinfect pig houses, playgrounds, appliances, vehicles, etc.
④ Strengthen nursing work, isolate sick pigs, suspend feeding and feeding, and take symptomatic treatment.
Which animals can be detected by the BALLYA Gastroenteritis test?
The BALLYA Gastroenteritis test can be applied to all kinds of pigs. The characteristics of this product are short detection time, simple operation and low price.
Significance of testing Gastroenteritis
In order to ensure the safety of consumers, reduce the economic loss of the owner of the breeding plant, prevent the sick pigs from entering the market, and treat the sick pigs in time, the Gastroenteritis Test can play a key role. Ensure the safety of farm owners and consumers, reduce unnecessary losses.
Component of BALLYA Gastroenteritis Test?
BALLYA Gastroenteritis Test
, 20 cassettes
Swab, 20 pcs
PE Groves, 1 packet
Sample Buffer, 20 vialsMini Pipette
Disposable micropipette tips (optional)
Kit Instruction, 1pcs
How to use BALLYA Gastroenteritis Test?
1. Collect a freshly excreted stool sample with a cotton swab, or take a sample directly from the rectum. If screening for rotavirus in animals without diarrhea, use rectal sampling. Dry samples are not recommended; it often results in false positives due to excessive dry sample collection.
2. Insert the swab to the test tube immediately. Spin the swab until the reagent mix with the sample (not less than 10 times). Squeeze the swab on the inner tube wall to squeeze out the liquid.
3. Stand for 1-2mins to dissolve the big particle.
4. Put the test card on the flat table, absorb the supernatant and add 5 drop into the sample well carefully.
5. The liquid will flow the screening hole, if not, please softly squeeze the area between sample well and screening hole.
6. Stand for 10-15mins at room temperature and read the result. The result after 15 minutes is invalid.
Limitations of BALLYA Gastroenteritis Test?
BALLYA Gastroenteritis Test is a qualitative test kit. It’s only for screening purpose. If have positive cases or suspected case, maybe use other detection method to make a further detection, such as, ELISA, PCR, qPCR, etc.
Pigs are most susceptible to porcine infectious gastroenteritis virus. Pigs of all ages can be infected, thus posing a huge economic threat to pig farming.BALLYA Gastroenteritis Test
provided by BALLYA
can effectively detect whether pigs have transmissible gastroenteritis of swine. This kit is not only simple to operate, but also has a short test time and high accuracy. Allow the veterinarian to respond accordingly.