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Kanamycin Test

Kanamycin-Test-Kit

For Milk, Milk Powder, Pasteurized Milk

Specs:96T / box
Testing Time:7-10 mins
Regulation: CN / EU / RU / US

Operation video

Instrution For Kanamycin Test

Introduction
Single Test Kit, rapid to detect an antibiotic residues in milk and dairy product by using colloidal gold immunochromatography technology. As soon as 7-10 minutes to results.

Application
For raw milk, whole milk powder, Pasteurized milk.

Spicifications
1. Specificity
Not cross-react with macrolides, trimethoprim, sulfonamides drugs, etc.

2. Limit of Detection
Refer to instruction.

Storage & Shelf-Life
At 2~8℃ out of sunlight, 12 months

Compolents (96T / box)
Instruction                          1
Tube                                     12

Equipments (Option)
Timer
Incubator
Mini Pipette(200μL)
Tips for mini pipette
Reader

Operations
1. Take 200 μL milk specimen, add into micro well, mix with the pink agent 10 times.
Remark: avoid specimen residues too much in the well.
2. Incubate 3 minutes
3. Insert strips into the wells
4. Incubate 5 minutes
5. Take out strips and read results by eye or reader.

Interpretation
Visual inspection
1. If C line visible, interpret according to the instruction. T line compare with C line to interpret positive or negative.
2. If C & T are invisible, it’s invalid detection, repeat testing again.

Reader Interpretation
Refer to the instruction

Results Illustration

If you want to know more, please contact us

Trivia questions: What is Kanamycin?

Kanamycin

Kanamycin is an inhibitor of protein biosynthesis that causes misreading of the mRNA code by binding to the 30S ribosome. If an enzyme that destroys kanamycin is produced in bacteria, it can become a resistant strain. Kanamycin-resistant plasmids are often used in molecular cloning as selection or marker genes.

Kanamycin concentration

Generally, the working concentration of ampicillin for sieving E. coli is 50 ug / mL, with 50 mg / mL, diluted 1000 times when used, and the working concentration of 30 kug / mL with anamycin, and diluted with 1000 times when used with 30 mg / mL, depending on the nature of the specific plasmid. Go up a bit. You can use 100 for amp and 50 for kanamycin.

Kanamycin resistance

Kanamycin is used as a selection agent in molecular biology and is most commonly used to isolate genes that have absorbed the binding to genes that encode kanamycin resistance (mainly neomycin phosphotransferase II [NPT II /]) (Eg, plasmid) bacteria (eg, E. coli) Neo]). Bacteria that have been transformed with a plasmid containing a kanamycin resistance gene are inoculated on kanamycin (50-100 ug / ml) containing agar plates or on kanamycin (50-100 ug / ml) In the medium. Only bacteria that successfully absorb the kanamycin resistance gene are resistant and grow under these conditions. Kanamycin is a powder, white to off-white, and soluble in water (50 mg / ml).

How does kanamycin kill bacteria

Kanamycin mode of action

Kanamycin works by interfering with protein synthesis. It binds to the 30S subunit of the bacterial ribosome. This leads to misalignment with the mRNA and ultimately misreading, which leads to putting the wrong amino acid into the peptide. This results in non-functional peptide chains.

What is kanamycin used for?

Kanamycin antibiotic

Kanamycin (also known as kanamycin A) is an aminoglycoside bactericidal antibiotic that is available orally, intravenously, and intramuscularly and can be used to treat a variety of infections. Kanamycin is isolated from Streptomyces kana, and its most commonly used form is kanamycin sulfate.

Kanamycin sulfate

Kanamycin sulfate is an aminoglycoside broad-spectrum antibiotic with an antibacterial spectrum similar to neomycin. It is mainly for serious infections caused by Gram-negative bacteria such as Escherichia coli, Klebsiella, Proteus, Pneumoniae, Enterobacter aerogenes, and Shigella, etc. Antibacterial effect. It is mainly used clinically for lung infection, urinary tract infection, biliary infection, sepsis, and abdominal infection caused by sensitive bacteria. The latter two are often combined with other antibacterial drugs.

Kanamycin for fish

Kanamycin is a very effective antibiotic that can be used to treat popular eyes, edema, septicaemia, rotten fins, other fungal and bacterial fish infections. Safe because it does not affect your biofilter and is easily removed with activated carbon.

Kanamycin side effects

Those with higher rates have hearing loss, tinnitus, or fullness in the ears (ototoxicity), hematuria, decreased urination or decreased urine output, decreased appetite, extreme thirst (nephrotoxicity), instability, dizziness (ototoxicity, Affecting vestibular), nausea or vomiting (ototoxicity, affecting vestibular, renal toxicity). Less frequent cases have difficulty breathing, lethargy, or weakness (neuromuscular block, nephrotoxicity). Hearing loss, tinnitus, or fullness in the ears after discontinuation of the drug indicates that ototoxicity may be needed and attention should be paid. May have hearing and kidney damage. Can cause gastrointestinal reactions: nausea, vomiting, loss of appetite, bloating, diarrhea. Occasionally allergic reactions, rashes, drug fever, etc. In severe cases, there may be shock death. Neurological symptoms, Myocardial depression, respiratory failure, etc.

Summary

Kanamycin can be used for oral and intravenous injection, and is effective against a variety of bacterial infections. Now kanamycin is often used as a veterinary antibiotic in dairy farming, resulting in more and more serious kanamycin residues in milk. The kanamycin rapid test kit independently developed by Ballya can effectively deal with such situations and prevent milk with kanamycin residue from flowing into the market. Ballya's rapid test kit is not only easy to operate, but also has short time and high accuracy, and the test results comply with EU standards.

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