BALLYA Tetracyclines Test is manufactured by BALLYA, it’s rapid test for detection tetracyclines residues in chicken meat, pork and bee porduct, especially in pork. Lateral flow assay test base on the gold immunochromatography assay technology.
Tetracycline is the most basic compound in the tetracycline family of antibiotics. It and its salts are yellow or light yellow crystals. It is extremely stable in the dry state. Except for chlortetracycline, the aqueous solutions of other tetracycline families are quite stable. The tetracycline family is soluble in dilute acids, dilute alkalis, etc., slightly soluble in water and lower alcohols, but insoluble in ethers and petroleum ethers.
Tetracycline antibiotics mainly include chlortetracycline, oxytetracycline, and tetracycline. Tetracycline antibiotics have a common chemical structure. Both chlortetracycline and oxytetracycline are derivatives of tetracycline. The former is chloretracyelin and the latter is oxyeracyein.
Tetracyclines mechanism of action
Tetracycline antibiotics form a reversible conjugate with the 30S subunit of the bacterial inner ribosome to inhibit protein synthesis and play an antibacterial effect. When the antibiotic concentration is low, this reversible competitive binding will also be useless, and bacterial protein synthesis will continue. Tetracycline can also inhibit mitochondrial protein synthesis by binding to the mitochondrial 70S subunit. The binding ability of tetracycline to the 80S subunit of eukaryotic cells is relatively weak, so the ability to inhibit protein synthesis in eukaryotic cells is also weak.
Tetracycline and Doxycycline are components of a broad-spectrum antibiotic called tetracycline antibiotics. All antibiotics in this class of drugs are derived from the same four-carbon ring basic structure. However, the specific ring structure of each drug is slightly different and should be recommended based on pharmacokinetics and bacterial resistance. Although doctors use these two drugs to treat many of the same infections, they have proven that each drug is often more effective in certain applications.
The main difference between tetracycline and doxycycline is the difference in pharmacokinetics. Pharmacokinetics refers to the body's response to specific drugs. This includes how the body disperses and metabolizes the drug, and even excludes it eventually. For example, the body tissues that are metabolized by tetracycline are different from doxycycline. The metabolic concentration of doxycycline reaches the highest in the eyes, while the essence of tetracycline is mainly in body fluids.
How Tetracyclines uses for?
Tetracycline is used to diagnose malignant tumors, various cysts, treat refractory pleural effusion, treat bronchopleural fistula, treat underarm odor, treat internal hemorrhoids, and treat acne with tetracycline. Yellow teeth may occur in children, tooth discoloration and bone growth inhibition may occur in pregnant women. Therefore, children and pregnant women should be cautious or prohibited from taking this drug.
Mainly used for skin surface infections caused by sensitive gram-positive bacteria and gram-negative bacteria. Tetracycline dosage: oral, commonly used for adults: 0.25-0.5g once, once every 6 hours. Common dosage for children over 8 years old: 25-50 mg/kg/day, divided into 4 dosages. The course of treatment is generally 7-14 days. Mycoplasma pneumonia and brucellosis take about 3 weeks.
Mainly used for the following diseases (1) Rickettsia, including epidemic typhus, endemic typhus, Rocky Mountain fever, tsutsugamushi and Q fever. (2) Mycoplasma infection. (3) Chlamydia infection, including psittacosis, sexually transmitted diseases, lymphadenoma, non-specific urine.
What's Tetracyclines side effect?
(1) Gastrointestinal reaction: Direct stimulation after oral administration can easily cause symptoms such as nausea, vomiting, abdominal pain, diarrhea, abdominal discomfort, and appetite loss. Although it can be reduced after a meal, it affects the absorption of drugs. The local irritation is large and can even cause esophageal ulcers. (2) Double infection (alternating flora): often occurs in patients with frailty, infants and combined glucocorticoids and anti-tumor drugs. (3) Impact on bone and tooth growth (4) Liver damage (5) Vitamin deficiency (6) Renal toxicity In addition, tetracycline antibiotics can occasionally cause allergic reactions such as drug fever and rash.
It should be taken on an empty stomach to avoid any effects on food; It is strictly forbidden to reuse after it expires; The yellowing of the teeth will occur in children. The pregnant women may have discolored teeth and inhibit bone growth. Therefore, children and pregnant women should take this medicine with caution or take it forbid.
Tetracyclines use as a veterinary medicine
Although veterinary tetracycline antibiotics have a wide range of effects, it should be noted when using them, and the appropriate amount should be applied in time, because the toxic and side effects of this drug, the adverse reactions of tetracycline drugs: mainly local irritation, double infection and damage to the liver, etc. Local irritation manifests as oral antibiotics of this type, which directly stimulate the gastrointestinal mucosa; after intramuscular injection, the hydrochloride is strongly acidic, which can cause local pain, inflammation, and necrosis; intravenous injection can produce phlebitis and thrombosis. Chloramphenicol is the strongest of these irritants, followed by oxytetracycline, and tetracycline is the lightest.
Tetracyclines for fish
Low concentration is bacteriostatic, and high concentration is sterilization. It has no effect on viruses, fungi and Pseudomonas aeruginosa. The effect on Gram-positive bacteria is close to that of penicillins, and the effect on Gram-negative bacteria is similar to that of chloramphenicol. It is used to prevent and treat bacterial diseases such as fish enteritis, red skin and rotten gills.
Tetracyclines for dogs
Canine enteritis medication. Tetracycline (10-20 mg / kg, twice a day for 1 to 2 weeks) can obtain satisfactory results. In some cases, metronidazole (10-20 mg / kg, twice a day, continued medication for 1 to 2 weeks) is better because it also has the effect of suppressing obligate anaerobic bacteria.
Tetracycline for chickens
Tetracycline has a greater side effect on chickens, which not only stimulates the digestive tract and damages the liver, but also can combine with calcium ions, magnesium ions and other metal ions in the chicken digestive tract to form a complex and hinder calcium absorption. The calcium ions in the plasma combine to form insoluble calcium salts that are excreted from the body, thereby depriving the chicken of calcium, which hinders the formation of eggshells, leading to the production of soft-shelled eggs, poor egg quality, and egg production. The rate drops. Therefore, when using tetracycline antibacterial drugs, it should avoid feeding soybean, black beans and their cakes, and should not be fed with feed additives such as stone powder, bone powder, eggshell powder and gypsum.
What specimen can be detected by BALLYA Tetracyclines Test?
Specimen include: chicken meat, pork and bee porduct etc. Widely used in chicken meat and pork processing industry.
Significance of testing Tetracyclines
To protect human have health food and avoid contaminated food. The tetracyclines is harmful for human being, can not take too much in body.
1. Take chicken meat specimen, bee porduct specimen, pork specimen and extrat supernatant 2. Add the supernatant to cassette 3. Wait for 15 minutes to result 4. The details of operation, refer to the kit instruction
Limitations of BALLYA Tetracyclines Test?
BALLYA Tetracyclines Test is a qualitative test kit. It’s only for screening purpose. If have positive cases or suspected case, maybe use other detection method to make a further detection, such as, ELISA, PCR, qPCR, etc.
As a veterinary drug, this product is absorbed orally quickly but not completely. It is widely distributed in animals and can enter tissues and body fluids well. Mainly excreted from urine in the form of original drugs. But it is inevitable that there will be residual drugs left in the body. This is likely to cause tetracyclines residues in chicken meat, pork and bee porduct. It is necessary to detect tetracyclines in chicken meat, pork and bee porduct. BALLYA Tetracyclines Test provided by BALLYA can effectively and quickly detect whether chicken meat, pork and bee porduct contain tetracyclines residues. Not only is the operation simple and the detection time is short, it allows the tester to obtain the test results in the first time.