BALLYA Pefloxacin Test is manufactured by BALLYA, it’s rapid test for detection Pefloxacin residues in egg & milk product, especially in milk. Lateral flow assay test base on the gold immunochromatography assay technology.
Start with the preparation of the intermediate boron chelate in norfloxacin. The boron chelate compound, acetonitrile, N-methylpiperazine and triethylamine were mixed, and reacted at room temperature before refluxing. The solvent was distilled off, water was added, and concentrated hydrochloric acid was adjusted to pH = 3 before refluxing. Add activated carbon to decolorize and filter. The filtrate was basified to pH = 11, and then activated carbon was decolorized, filtered, and neutralized with 30% acetic acid to pH = 6.7-7.2, overnight in the refrigerator. Filter, wash with water, and dry to obtain off-white pefloxacin.
Pefloxacin mechanism of action
This product has a broad-spectrum antibacterial effect and has a good antibacterial effect on the following bacteria: most bacteria of the family Enterobacteriaceae, including Escherichia coli, Klebsiella, Proteus, Shigella, Typhoid and Salmonella Etc. and Haemophilus influenzae, Neisseria and so on. It also has a certain antibacterial effect on Pseudomonas aeruginosa and Staphylococcus aureus. It has only mild effects on pneumococcus, streptococci and enterococci. In addition, it has antibacterial activity against leprosy. Pefloxacin mesylate is a bactericide, which acts on the A subunit of bacterial DNA helicase to inhibit DNA synthesis and replication and cause bacterial death.
How Pefloxacin uses for?
What does pefloxacin tablets treat?
Pefloxacin indication: Suitable for respiratory tract, urinary tract infections, gonorrhea, prostatitis, adult G-bacteria and staphylococcus serious infections caused by sensitive bacteria such as sepsis, endocarditis, bacterial meningitis, respiratory tract, urethra, kidney, otolaryngology infections, Gynecological diseases, abdominal, hepatobiliary, osteoarthritis and skin infections.
Pefloxacin dosage
Oral, 0.4g once, twice a day. The first dose can be doubled. Intravenous drip 0.4g once, twice a day. Dissolved in isotonic glucose injection. The infusion should not be too fast, drip after one hour. Adults and intravenous doses are 400 mg each time, once every 12h, the dose of urinary tract infections and other lighter infections should be reduced. Each time 400 mg of the product is intravenously infused, it needs to be diluted with 250ml of 5% glucose solution and then slowly dropped in the dark for at least 1h. It cannot be diluted with physiological saline or other chlorine-containing solutions to prevent precipitation. For patients with ascites and jaundice, take the medicine every two days.
What's Pefloxacin side effect?
1. Gastrointestinal reactions are more common and can manifest as abdominal discomfort or pain, diarrhea, nausea, or vomiting. 2. Central nervous system reactions may include dizziness, headache, drowsiness or insomnia. 3. Allergic reactions: rash, itchy skin, and occasionally exudative erythema multiforme and angioedema. A few patients have photosensitive reactions. 4. Occasionally occur: (1) Seizures, mental disorders, restlessness, confusion, hallucinations, tremor. (2) The manifestations of interstitial nephritis such as hematuria, fever, and rash. (3) Crystallized urine is more common in high-dose applications. (4) Joint pain. 5. A few patients can have elevated serum aminotransferase, increased blood urea nitrogen, and decreased white blood cells in the peripheral blood, which are mostly mild and transient.
Pefloxacin contraindications
(1) Patients with allergies to this product and fluoroquinolones are prohibited; (2). Because Escherichia coli is currently more resistant to fluoroquinolones, urine culture specimens should be retained before administration, and the medication should be adjusted with reference to the results of bacterial susceptibility; (3) Crystalline urine may occur when the product is used in large doses or when the urine pH is above 7; (4) For those with impaired renal function, the dosage should be adjusted according to renal function; (5) Application of fluoroquinolones can cause moderate to severe photosensitivity reactions; (6) When the liver function declines, it is necessary to weigh the pros and cons before applying, and adjust the dosage; (7) Patients with original central nervous system disease should avoid application.
What are the pefloxacin products?
Pefloxacin injection
Colorless or slightly yellow, yellowish green clear liquid. Indications: Various infections caused by pefloxacin-sensitive bacteria: urinary tract infections; respiratory tract infections; ear, nose, and throat infections; gynecological and reproductive system infections; abdominal and liver and gallbladder infections; bone and joint infections; Skin infections; sepsis and endocarditis; meningitis.
Pefloxacin tablet
Pefloxacin tablets are fluoroquinolones, a new type of fluoroquinolone antibacterial drugs, against G- and G + bacteria, including Enterobacteriaceae, Pseudomonas aeruginosa, Acinetobacter, Haemophilus, Neisser Staphylococcus and Staphylococcus (including methicillin-resistant strains) have a broad spectrum of activity. Its resistance to Staphylococcus aureus is similar to that of vancomycin, but its resistance to Pseudomonas aeruginosa is not as good as ciprofloxacin and cemetazidine, and it is resistant to some multivalent drug-resistant strains and methicillin Medicine bacteria are also effective.
What specimen can be detected by BALLYA Pefloxacin Test?
Specimen include: egg, milk, etc. Widely used in egg industry and dairy industry.
Significance of tesing Pefloxacin
To protect human have health food and avoid contaminated food. The pefloxacin is harmful for human being, can not take too much in body.
1. Take egg or milk specimen 2. Add the supernatant to cassette 3. Wait for 15 minutes to result 4. The details of operation, refer to the kit instruction
Limitations of BALLYA Pefloxacin Test?
BALLYA Pefloxacin Test is a qualitative test kit. It’s only for screening purpose. If have positive cases or suspected case, maybe use other detection method to make a further detection, such as, ELISA, PCR, qPCR, etc.
Conclusion
As a veterinary drug, this product is absorbed orally quickly but not completely. It is widely distributed in animals and can enter tissues and body fluids well. Mainly excreted from urine in the form of original drugs. But it is inevitable that there will be residual drugs left in the body. This is likely to cause pefloxacin residues in chicken or eggs. It is necessary to detect pefloxacin in eggs. BALLYA Pefloxacin Test provided by BALLYA can effectively and quickly detect whether eggs contain pefloxacin residues. Not only is the operation simple and the detection time is short, it allows the tester to obtain the test results in the fsirst time.
