BALLYA Oxolinic Acid Test is manufactured by BALLYA, it’s rapid test for detection Oxolinic Acid residues in egg & pork product, especially in pork. Lateral flow assay test base on the gold immunochromatography assay technology.
Oxolinic Acid is the second generation of quinolones are white with yellowish white columnar crystals or crystalline powder, odorless and tasteless. Melting point 320 ℃, almost insoluble in water and ethanol, soluble in formic acid and sodium hydroxide solution, does not absorb moisture, and is relatively stable to heat, humidity and light.
Oxolinic acid mechanism of action
Targets the deoxyribonucleic acid (DNA) of bacteria. The double-stranded DNA of bacteria is twisted into loops or spirals (called supercoils). The enzyme that makes DNA supercoils is called DNA gyrase, and quinolones hinder this enzyme, further causing irreversible damage to chromosomes and causing bacterial cells No longer split. They show selective toxicity to bacteria. At present, the resistance of some bacteria to many antibiotics can be widely spread due to plasmid transmission.
Oxolinic acid solubility
It is almost insoluble in water and ethanol, soluble in formic acid and sodium hydroxide solution, does not absorb moisture, and is relatively stable to heat, humidity and light.
How Oxolinic Acid uses for?
It has a strong broad spectrum, has a strong antibacterial effect against Gram-negative bacteria and some positive bacteria, and has no cross-resistance with antibiotics, but has no antibacterial effect on fungi and Mycobacterium tuberculosis. It has many advantages such as good bacteria effect. Foreign aquaculture farmers believe that it is one of the more ideal drugs for the treatment of aquatic animal diseases, and it has strong antibacterial activity against fish pathogens such as Vibrio anguillarum and Aeromonas hydrophila.
Oxolinic acid indications
Antibacterial drugs that mainly act on Gram-negative bacteria have a weaker effect on Gram-positive bacteria (some varieties have better antibacterial effects on Staphylococcus aureus).
What's Oxolinic Acid side effect?
The main side effects of this class of drugs are: ① Gastrointestinal reactions: nausea, vomiting, discomfort, pain, etc.; ②Central reactions: headache, dizziness, poor sleep, etc., and may cause mental symptoms; ③Because this class of drugs can inhibit the effect of γ-aminobutyric acid (GABA), it can induce epilepsy, and those with a history of epilepsy should be used with caution; ④ This type of medicine can affect cartilage development, pregnant women and minor children should be used with caution; ⑤ Crystalline urine can be produced, especially in alkaline urine; ⑥ Large-dose or long-term application of this class of drugs is likely to cause liver damage.
Oxolinic acid toxicity
Acute oral LD50630mg / kg (male), 570 mg / kg (female) for rats; acute oral LD502200mg / kg for male mice, 1450 mg / kg for female mice; acute percutaneous LD50 for rats> 2000 mg / kg, it has no stimulating effect on the skin of Chemicalbook, and has a slight stimulating effect on the eyes. No sensitization. The no-effect dose of 100-300 mg / kg in the 2-year feeding test in rats. Animal experiments have no teratogenic, cancer-causing, and mutagenic effects. It is used as usual and has no effect on fish. Carp LC5010mg / L (48h).
Oxolinic acid interaction
(1) Alkaline drugs, anticholinergic drugs, and H2 receptor blockers can all reduce the acidity of gastric juice and reduce the absorption of this type of drugs. (2) Rifampicin (inhibitor of RNA synthesis) and chloramphenicol (inhibitor of protein synthesis) can reduce the effects of this class of drugs, completely eliminate the effects of nalidixic acid and haloperic acid, and make fluazamic acid effect of ciprofloxacin is partially offset. (3) Fluoroquinolones can inhibit the metabolism of theophylline. When combined with theophylline, the plasma concentration of theophylline will increase, and the toxic reaction of theophylline may occur, so attention should be paid.
Oxolinic Acid use as a veterinary medicine
Oxolinic acid for fish
Overseas aquaculture farmers believe that it is one of the more ideal drugs for the treatment of aquatic animal diseases, and it has strong antibacterial activity against fish pathogens such as Vibrio anguillarum and Aeromonas hydrophila. Therefore, eels and other aquatic animals are widely used in Japan and Taiwan Province. PH in marine aquaculture has a slight impact on it, and the dosage of drugs needs to be increased in alkaline environments. The toxicity of oxolinic acid is very slight, acute, and the half-lethal amount of oral administration to carp (that is, the time that 50% of individuals can die under the influence of a certain concentration of the drug) is more than 4000 mg / kg. The use of the drug must be stopped before 25 days, otherwise the residual amount exceeds the standard, and the Japanese market is prohibited from importing.
What specimen can be detected by BALLYA Oxolinic Acid Test?
Specimen include: pork, egg, etc. Widely used in pork industry and dairy industry.
Significance of tesing Oxolinic Acid
To protect human have health food and avoid contaminated food. The Oxolinic Acid is harmful for human being, can not take too much in body.
1. Take pork specimen and extrat supernatant 2. Add the supernatant to cassette 3. Wait for 15 minutes to result 4. The details of operation, refer to the kit instruction
Limitations of BALLYA Oxolinic Acid Test?
BALLYA Oxolinic Acid Test is a qualitative test kit. It’s only for screening purpose. If have positive cases or suspected case, maybe use other detection method to make a further detection, such as, ELISA, PCR, qPCR, etc.
Oxolinic acid has been widely used as an anabolic hormone in various industries. The pig breeding industry deserves special attention. Pork provides people with rich nutrition, making it indispensable in daily life. The problem of oxolinic acid hormone residues in pork has always plagued people, so it is necessary to detect pork in time. BALLYA Oxolinic Acid Test provided by BALLYA can effectively and quickly detect the presence of oxolinic acid residues in pork. Not only the operation is simple, the test time is short, but the tester can get the test result in the first time.