BALLYA Nicarbazin Test

Ballya-antibiotic-testBALLYA Nicarbazin Test

What is BALLYA Nicarbazin Test?

BALLYA Nicarbazin Test is manufactured by BALLYA, it’s rapid test for detection nicarbazin residues in chicken meat & chicken meat product, especially in chicken meat. Lateral flow assay test base on the gold immunochromatography assay technology.
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What is Nicarbazin?

It is yellow or yellow-green powder; odorless and slightly odorous. Used to prevent coccidiosis in chickens and turkeys.

Nicarbazin solubility

This product is slightly soluble in dimethylformamide, but insoluble in water, ethanol, ethyl acetate, chloroform and ether.

Nicarbazin mode of action

Used to prevent coccidiosis in chickens and turkeys. This product is effective for the second generation of schizonts of coccidia. The recommended amount does not affect the immunity of chickens against coccidia, and it is safe. Use with caution in hot seasons and ban during egg production. Mixed feed, 125g per 1000 kg feed. The two components of nicarbazin can be absorbed by the digestive tract of poultry, and are widely distributed in tissues and body fluids.

Nicarbazin synthesis

Add 8.1g (0.027mol) DNC, 2.5g (0.042mol) urea, 4mL acetylacetone and a small amount of concentrated hydrochloric acid to 35mL absolute ethanol, stir and reflux to react for 10-15h, cool to room temperature for crystallization, centrifuge, no Chemicalbook water washed with ethanol and dried to obtain a yellow composite powder, the yield is 80%. The molar ratio of 4,4'-p-nitrodiphenylurea (DNC) and 2-hydroxy-4,6-dimethylpyrimidine (HDP) is 1: 1 compound. HDP is prepared by the condensation reaction of acetylacetone and urea.

How Nicarbazin uses for?

What's Nicarbazin side effect?

The main reasons why the content of chicken is detected excessively frequently are:
The main ingredient is difficult to metabolize. Nicarbazine is a mixture of two types of substances, 4-4 ’dinitrodiphenylurea (DNC) and 2-hydroxy-4.6-dimethylpyrimidine (HDP), which can be digested and absorbed by the digestive tract
It is collected and widely dispersed in animal tissues and body fluids. Among these two components, HDP basically has no anticoccidial effect, and can be quickly excreted through urine in animals; DNC is the main active component of anticoccidial effects. It is metabolized slowly in animals and can only be excreted through feces.

Overuse. Many breeders apply pesticides at random or do not strictly implement the drug withdrawal period, resulting in excessive DNC content in animals.

Wide source of intake. Although it is stipulated that there should be a 4-day drug withdrawal period before slaughtering of broilers, to ensure the complete metabolism of nicarbazin in chickens, it is still possible for broilers to enter the broiler circulation during the drug withdrawal period because they can contact their feces.

Nicarbazin use as a veterinary medicine

Nicarbazin in feeds

This product is effective against the second generation of schizonts of coccidia and is used to prevent coccidiosis in chickens and turkeys. Mixed feed, 125g per 1000 kg feed.

Nicarbazin toxicity in broilers

Nicarbazin poisoning in broilers generally rarely occurs. Once the disease occurs, the situation is urgent and the treatment is more difficult. To find a way to cool down and solve the symptoms of high fever.
Chickens are mentally exhausted, fasting, fasting, breathing with their mouths open, their heartbeats are accelerated, their body temperature rises, they stay on the ground, they flutter their wings, scream, and struggle before death.

Nicarbazin Precautions

(1) During the preventive treatment of nicarbazine, if a large number of chickens are exposed to infectious oocysts and coccidiosis breaks out, they should be quickly switched to more effective drugs (such as toltrazuril, sulfonamides, etc.)
(2) Because nicarbazine can reduce egg production rate, fertilization rate, egg quality and lighten the brown eggshell, laying hens are prohibited.
(3) Due to the potential growth inhibitory effect of nicarbazine on chicks, it is advisable not to use young chicks under 5 weeks of age.
(4) During the intense heat, if the ventilation and cooling equipment of the chicken house is incomplete and the room temperature exceeds 40 ℃, the application of nicarbazine can increase the mortality of the chicks.
(5) Withdrawal period. Broiler for 4 days.

What specimen can be detected by BALLYA Nicarbazin Test?

Significance of testing Nicarbazin

Significance of testing Nicarbazin

To protect human have health food and avoid contaminated food. The Nicarbazin is harmful for human being, can not take too much in body.

Component of BALLYA Nicarbazin Test?

BALLYA Nicarbazin Test, 25 cassettes
Sample Buffer, 1 vial
Mini Pipette (optional)
Disposable micropipette tips (optional)
Kit Instruction, 1pcs
Nicarbazin Test

How to use BALLYA Nicarbazin Test?

1. Take chicken meat specimen
2. Add the supernatant to cassette
3. Wait for 15 minutes to result
4. The details of operation, refer to the kit instruction

Limitations of BALLYA Nicarbazin Test?

BALLYA Nicarbazin Test is a qualitative test kit. It’s only for screening purpose. If have positive cases or suspected case, maybe use other detection method to make a further detection, such as, ELISA, PCR, qPCR, etc.


At present, studies on the physiologic toxicity of nicarbazin show that when 80 times the normal dose is taken for a long time, the sperm aberration rate of male rats increases significantly. Therefore, we should pay attention to control the dosage, in actual use
It is safe to use within the dosage range. Long-term consumption of chicken meat with excessive levels of such drugs may pose a certain risk to health. The detection of nicarbazin is very necessary.
BALLYA Nicarbazin Test provided by BALLYA can effectively and quickly detect whether chicken contain nicarbazin residues. Not only is the operation simple and the detection time is short.

Where to buy BALLYA Nicarbazin Test?

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