BALLYA Florfenicol Test is manufactured by BALLYA, it’s rapid test for detection florfenicol residues in chicken meat, or pork, especially in pork. Lateral flow assay test base on the gold immunochromatography assay technology.
Florfenicol is a synthetic monofluoro derivative of Thiamphenicol, which is white or off-white crystalline powder, odorless. Antibiotic drugs produce broad-spectrum antibacterial effects by inhibiting the activity of peptide acyltransferase, and have a broad antibacterial spectrum, including various Gram-positive and negative bacteria and mycoplasma. Sensitive bacteria include Haemophilus bovine and swine, Shigella dysenteriae, Salmonella, Escherichia coli, Pneumococcus, Influenza bacillus, Streptococcus, Staphylococcus aureus, Chlamydia, Leptospira, Rickettsia, etc. It is a new broad-spectrum antibacterial drug of chloramphenicol for veterinary use successfully developed in the late 1980s.
1. The nitro group (NO2) determines the activity of the raw drug. Idiosyncratic aplastic anemia is related to this. There is no such side reaction after substitution with methanesulfonic acid group. 2. After F replaces OH, it does not cause bone marrow suppression or aplastic anemia. Enhance drug activity and reduce the generation of drug resistance. 3. Chloramphenicol-the first available synthetic antibiotic. The side effects are relatively large, and it has been banned from food animals. 4. Thiamphenicol-the upgrade of chloramphenicol, but it can still inhibit the production of red blood cells, and has a strong immunosuppressive effect. 5. Florfenicol-the upgrade of thiamphenicol, does not cause bone marrow suppression or aplastic anemia, and has stronger antibacterial activity.
It is easily soluble in dimethylformamide, soluble in methanol, slightly soluble in glacial acetic acid, and slightly soluble in water or chloroform.
Florfenicol mechanism of action
It can be diffused into bacterial cells through fat solubility, mainly acting on the 50s subunit of the bacterial 70s ribosome, inhibiting transpeptidase, hindering the growth of peptidase, inhibiting the formation of peptide chains, thereby preventing protein synthesis and achieving antibacterial purposes. This product has a broad antibacterial spectrum, and has strong effects on gram-positive and gram-negative bacteria and mycoplasma. This product has rapid oral absorption, wide distribution, long half-life, high blood concentration and long blood drug maintenance time.
How Florfenicol uses for?
Florfenicol was initially mainly used in aquaculture for the treatment of pseudonuclear pasteurosis and streptococcus disease of yellowtail, and the natural outbreak of the Atlantic salmonellosis. Animal-specific antibacterial drugs are used for bacterial diseases of pigs, chickens and fish caused by sensitive bacteria, especially for respiratory infections and intestinal infections.
Mixed drinking poultry: 3-4g 100L water, livestock, 20-25 mg per kilogram body weight, once a day for 3 days. Intramuscular injection: Chicken, pig, and cattle are weighed at 15-20 mg per kilogram of body weight, once every 48 hours, and used twice.
What's Florfenicol side effect?
Chloramphenicol has serious adverse reactions that cause aplastic anemia (Varma, et al. 1986). Many countries have banned the use of chloramphenicol in the prevention and treatment of animal diseases, especially food animals, such as the US FDA. Studies have shown that the para-nitro group on the aromatic ring in the structure of chloramphenicol is the main group causing aplastic anemia. Florfenicol uses H3C-SO2 instead of O2N in the structure and does not cause aplastic anemia. Therefore, florfenicol will replace chloramphenicol in the prevention and treatment of animal diseases, especially in food animals, and has broad application prospects in veterinary clinics.
Compatible with neomycin, doxycycline hydrochloride, colistin sulfate, lauricin, etc., the curative effect is enhanced. Compatible with ampicillin, cefradine, cephalexin, etc., the efficacy is reduced. Compatible with kanamycin, streptomycin, sulfonamides, and quinolones, the toxicity increases. Compatible with VB12, it will inhibit red blood cell production.
It has an effect on a variety of gram-positive bacteria and gram-negative bacteria and mycoplasma. Its antibacterial mechanism is mainly through binding to the 50S ribosomal subunit, inhibiting the key enzyme required for protein synthesis-peptide acyltransferase, thereby specifically preventing aminoacyl tRNA from binding to the receptor on the ribosome and inhibiting the peptide chain Extend and make the bacterial protein unable to be synthesized. The MIC for chloramphenicol and thiamphenicol-resistant Shigella dysentery, Salmonella typhi, Escherichia coli, and Ampicillin-resistant Haemophilus influenzae are all 0.5μg/ml. This product not only increases its water solubility, but also has a small loss of in vivo processes after oral administration and injection. The ester and florfenicol are released through metabolic decomposition of biological enzymes in the body, and the ester overcomes the resistance of bacteria. Florfenicol is targeted and positioned to exert the extremely effective effect of the drug on the organism. The dosage is small, the effect is fast, the half-life is long, the blood concentration is high, and the blood concentration can be maintained for a long time. Mainly excreted through the kidneys. Used in dosage forms such as soluble powder, oral liquid, injection, etc.
Precautions for Florfenicol
1. The bait after mixing should not be stored for a long time; 2. This product should be stored properly to avoid accidental consumption by humans and animals; 3. Discarded packaging after use should be properly disposed of.
Florfenicol use as a veterinary medicine
Florfenicol for dogs
Florfenicol can be used to treat dogs with cold and runny nose, can be used to treat bacterial respiratory infections, and is also effective for mycoplasma. There is no problem in treating common colds, but some viral infections and cold symptoms are similar, such as dog vice Flu, etc. At this time, the use of florfenicol has no effect, it is recommended to seek medical treatment in time.
Respiratory tract infections, mastitis, mastitis in cattle and sheep; infectious pleural pneumonia, swine asthma, piglet red, (yellow, white) dysentery, erythrozoonosis, streptococcal disease, edema disease, atrophic rhinitis, Pneumonia, erysipelas, sow mastitis and endometritis are very effective. Oral: 20-30 mg / kg for cattle and sheep (that is, 100g for 500 kg body weight) twice a day for 3-5 days.
Florfenicol in swine
It is used to prevent and treat swine asthma, infectious pleurisy pneumonia, atrophic rhinitis, swine pneumonia, and streptococcal disease. The curative effect is effective for Escherichia coli and other causes of yellow and white dysentery, enteritis, blood dysentery and edema disease of piglets.
Florfenicol in poultry
It is used to prevent poultry cholera caused by Escherichia coli, Salmonella, Pasteurella, etc., chicken diarrhea, diarrhea, stubborn diarrhea, yellow, white and green stools, watery feces, diarrhea, spotted or diffuse bleeding of intestinal mucosa, Chronic respiratory disease caused by omphalitis, pericardium, liver, bacteria, mycoplasma, infectious rhinitis, airbag turbidity, cough, tracheal rattling, dyspnea, etc.
What specimen can be detected by BALLYA Florfenicol Test?
Specimen include: chicken meat, or pork, etc. Widely used in chicken meat and pork industry.
Significance of testing Florfenicol
To protect human have health food and avoid contaminated food. The Florfenicol is harmful for human being, can not take too much in body.
1. Take chicken meat specimen, or pork specimen and extrat supernatant 2. Add the supernatant to cassette 3. Wait for 15 minutes to result 4. The details of operation, refer to the kit instruction
Limitations of BALLYA Florfenicol Test?
BALLYA Florfenicol Test is a qualitative test kit. It’s only for screening purpose. If have positive cases or suspected case, maybe use other detection method to make a further detection, such as, ELISA, PCR, qPCR, etc.
Florfenicol belongs to the class of amido alcohol antibiotics. It has good effects on many pathogens. It is also a commonly used medicine in chickens. It is used to treat E. coli, mycoplasma, chicken cholera, staphylococcus, and salmonella. However, many farms do not use florfenicol according to the standard, which may cause florfenicol residues in chicken and pork. Excessive intake of florfenicol is harmful to the human body. The detection of florfenicol is very necessary. BALLYA Florfenicol Test provided by BALLYA can effectively and quickly detect whether chicken and pork contain florfenicol residues. Not only is the operation simple and the detection time is short.