Furazolidone Test is used for testing Furazolidone residues in livestock, poultry and aquatic products, suitable for site rapid testing of all kinds of enterprises, testing agencies and supervision departments. The whole test procedure only takes 35min, easy to operate and read.
A. Ethanolamine is used as a raw material to condense with urea to obtain β-hydroxyethyl urea, which is nitrosated and cyclized to obtain 3-nitroso-2-azolidinone. Further reduction with iron powder and condensation with 5-nitro-2-furfuryl glycol diacetate and formaldehyde yield furazolidone.
B. Condensation of ethanolamine with urea, nitrosation and cyclization with sodium nitrite, and finally reduction with iron powder with 5-nitro-2-furfuryl glycol diacetate and formaldehyde to obtain furazolidone.
2. Brand Name
Furazolidone is a nitrofuran antibacterial agent and a monoamine oxidase inhibitor (MAOI). It is sold by the Roberts Laboratories under the trade name Furoxone and by GlaxoSmithKline as Dependal-M.
3. Mechanism of Action
Antibacterial effect on Gram-positive and negative bacteria, including Salmonella, Shigella, E. coli, Klebsiella pneumoniae, Enterobacter, Staphylococcus aureus, Enterococcus faecalis, Streptococcus pyogenes, Cholera Vibrio, Campylobacter and Bacteroides are also active against Trichomonas and Giardia under certain concentrations.
Its mechanism of action is to interfere with bacterial oxidoreductase and thereby block the normal metabolism of bacteria.
4. Uses
Furazolidone spray is specifically for the treatment of horses, for topical application in horses and ponies. For the prevention or treatment of superficial wounds, abrasions and lacerations caused by Staphylococcus aureus and Streptococcus. And Proteus. Sensitive to furazolidone.
Not suitable for animals intended for food. Use only as recommended by a veterinarian to treat puncture wounds, wounds that require surgical debridement or sutures, pride of meat, systemic and chronic infections of the skin, and chronic wounds of skin conditions associated with intense itching.
If redness, inflammation or swelling persists or worsens, discontinue use and consult a veterinarian. Wear gloves when washing or wash hands afterwards. Avoid contact with eyes.
Furazolidone veterinary
When used as a veterinary drug, furazolidone has a good effect on the prevention and treatment of certain protozoan diseases, water mold diseases, bacterial rotten gills, erythroderma, and bleeding diseases.
In the breeding industry, furazolidone can be used to treat intestinal infections in livestock and poultry, such as piglet yellow and white pheasant. In the aquaculture industry, furazolidone has a certain effect on the infection of cerebral myxosoma by Salmonella.
Furazolidone is listed by the Ministry of Agriculture of the People's Republic of China as a banned drug and cannot be detected in animal foods.
1. Furazolidone for dogs
It is usually used to treat intestinal infections and coccidiosis in dogs, and it is effective against E. coli and dysentery.
2. Furazolidone for cats
For cat intestinal infections, coccidiosis, giardiasis have significant effects.
3. Furazolidone for fish
Furazolidone is the main component of tetriline, an ornamental fish medicine "yellow powder", which can interfere with the bacterial sugar metabolism process and oxidase system and exert bacteriostatic or bactericidal effects.
So it can be used to treat bacterial diseases of ornamental fish disease. And the effect is very good.
The hazards of Furazolidone
1. Side effects
Mainly nausea, vomiting, diarrhea, headache, dizziness, drug fever, rash, anal itching, asthma, orthostatic hypotension, hypoglycemia, pulmonary infiltration, etc. Occasionally hemolytic anemia, jaundice and polyneuritis may occur.
Excessive use of furazolidone may cause gastrointestinal reactions (such as nausea, vomiting, anorexia, diarrhea, and generally mild reactions); allergic reactions such as hemolytic anemia, rash, drug fever; polyneuritis; newborns and G-6- PH deficiency can cause hemolytic anemia.
Overdose or long-term continuous medication can cause animal poisoning and severely cause animal death. It can cause irreversible damage to the central nervous system, and can cause diseases such as hemolytic liver necrosis in the human body.
As early as 2002, the Ministry of Agriculture listed it as a banned veterinary drug, and it is strictly prohibited to use it in any food animal. However, in order to pursue economic benefits, some farmers still use it as a special medicine for treating aquatic gastrointestinal diseases.
Studies have also shown that furazolidone for long or high doses has varying degrees of toxic and side effects on liver, kidney, heart, hypothalamus and reproductive systems of animals.
2. Interaction
A. Combination with tricyclic antidepressants can cause acute toxic psychosis and should be avoided.
B. This product can enhance the effect of levodopa.
C. Sympathomimetic amines, tyramine-rich foods, appetite suppressants, monoamine oxidase inhibitors, etc. can enhance the effect of this product.
Features of Furazolidone Test
1. 15min reading results
2. Detection limits respond to EU and US FDA norms
3. Neither professionals nor equipment required
4. Easy to operate and read
Detection Method of Furazolidone Test
The detection of Furazolidone must be based on the detection of a series of metabolites.
Therefore, these metabolites AOZ are used as detection targets for abused furazolidone drugs.
AOZ Test is based on the competitive inhibition immune chromatography assay. During the lateral flow procedure, AOZ residues in extracted samples will bind to the colloidal gold-labeled specific monoclonal antibodies, inhibit the combination of antibodies.
Then the AOZ-BSA conjugates on the NC membrane, leading to color changes of the test line (line T).
Samples of Furazolidone Test
Samples include: pork, chicken, fish, shrimp and other aquatic products.
Detection Limit of Furazolidone Test
The LOD may change according to requirement or specimen's difference, please refer to kit instruction for further details.
Importance of Furazolidone Test
Furazolidone Test can help manufacturers, regulatory agencies, and the public monitor product quality and help humans protect their health.
Components of Furazolidone Test
1. Test Cards: 10 pcs
2. Extracting Reagent（6ml）:10 bottle
3. Reagent A（55ml）:1 bottle
4. Reagent B（6ml）:1 bottle
5. Reagent C（12ml）:1 bottle
6. Purifying Reagent（5ml）:2 bottles
7. Derivatization Reagent（2ml）:1 bottle
8. Re-hydration fluid（12ml）:1 bottle
9. Centrifuge Tubes（15ml）:20pcs
10. Product Instruction:1
How to use Furazolidone Test?
1. Prepare the sample. (Refer to the instruction.)
2. All reagents and Test Cards should be at room temperature before using. Please use the Test Cards within 1 hour.
3. Using a micropipette, inject 75ml of the sample into the sample hole (S hole).
4. Start the timer when the sample solution flows. After 10mins, interpret the test results according to the sketch picture. The result in the other time period is invalid.
Summary
Veterinary drugs play a very important role in the modern breeding industry, but in fact, some breeders or enterprises in order to save costs and obtain higher antibacterial effects, regardless of national regulations, repeatedly use antibacterial drugs or even use alternative veterinary drugs.
Although hydrogen nitrate is a broad-spectrum antibacterial drug, it has a bactericidal effect on most Gram-positive bacteria and Gram-negative bacteria, but research has found that these drugs are carcinogenic, mutagenic and teratogenic. Therefore, countries have listed it as an alternative veterinary drug.
The Furazolidone Test for Aquatic Products (0.5ppb) provided by BALLYA can effectively detect whether furazolidone is contained in the test object, ensuring the safety of consumers. The operation of the kit is very simple, and the detection time is short, the accuracy is high, and the test results comply with EU standards.
How to order Furazolidone Test?
Please check the online catalog and contact our sale representative via email: info@ballyabio.com or fill out contact form below:
