Chloramphenicol Test is used for testing Chloramphenicol residues in livestock, poultry and aquatic products, suitable for site rapid testing of all kinds of enterprises, testing agencies and supervision departments. The whole test procedure only takes 35min, easy to operate and read.
The most common mechanism of bacterial resistance to chloramphenicol is enzymatic inactivation primarily caused by chloramphenicol acetyltransferase, in some cases, acetylation of chloramphenicol phosphate transferase.
2. Mechanism of action
It prevents protein chain extension by inhibiting peptidyl transferase activity of bacterial ribosomes. It has a few A2451 and A2452 residues in the 23S rRNA of the 50S ribosomal subunit.
In combination with macrolide drugs, chloramphenicol directly interferes with substrate binding in the ribosome, while macrolide drugs spatially prevent alternative growth.
Chloramphenicol Uses
1. Chloramphenicol eye drops
Chloramphenicol eye drops are eye drops for treating eye diseases. It is mainly used to treat eyes caused by E. coli, Haemophilus influenzae, Klebsiella, Staphylococcus aureus, hemolytic streptococcus and other sensitive bacteria (such as trachoma, conjunctivitis, keratitis and blepharitis) Department of infection.
2. Chloramphenicol ear drops
Chloramphenicol ear drops are used for the treatment of external otitis, acute and chronic otitis media caused by sensitive bacterial infections.
3. Chloramphenicol ointment
Chloramphenicol ointment is a topical drug with chloramphenicol as the main ingredient. It is mainly used to treat bacterial infectious skin diseases, such as folliculitis, edema, impetigo, suppurative sweat glanditis, paronychia, cellulitis, etc.; also used to treat secondary bacterial infections caused by other skin diseases, Such as tinea pedis infection, eczema infection, scabies infection, etc.
4. Chloramphenicol antibiotic class
Chloramphenicol antibiotics can act on the 50S subunit of bacterial ribonucleoproteins and hinder the synthesis of proteins.
Because the binding of chloramphenicol to 70S ribosomes is reversible, it is considered a bacteriostatic antibiotic, but it can also have a bactericidal effect on some bacteria at high drug concentrations, even at lower concentrations of influenza bacilli. Produce bactericidal effect.
5. Chloramphenicol dosage
A. Oral: 0.5g for adults, once every 6 hours. A larger first dose can be used to treat typhoid fever. Patients should continue to apply for 2-3 days after normal body temperature to prevent recurrence, the highest dose does not exceed 26g.
Children per kilogram body weight 25 ～ 50 mg, divided dose once every 6 hours. Intramuscular injection or intravenous drip: 1day volume: 0.5-1g, divided into 2 injections. Dilute with liquid. One chloramphenicol (250 mg) should be diluted with at least 100ml.
B. Eye drops: 1-2 drops each time, 3-5 times daily. Ear drops: 2-3 drops each time, 3 times daily. Note: Chloramphenicol injection (containing solvents such as ethanol, glycerol or propylene glycol), It should be extracted with a dry syringe and shaken while diluting to prevent precipitation of crystals.
C. After the symptoms have subsided, the dose should be reduced or discontinued as appropriate.
The hazards of Chloramphenicol
If chloramphenicol is used improperly, it will cause aplastic anemia, which is generally divided into two types, that is, reversible erythroplasia inhibition and irreversible allergic aplastic anemia.
Reversible is due to the toxicity of chloramphenicol, which is related to the dose. If chloramphenicol is used more than a certain amount, it will become ill. Nitrophenyl radicals in the structure of chloramphenicol can inhibit the synthesis of mitochondrial proteins in blood cells, leading to damage to mitochondria.
Due to individual differences, there is currently no absolutely safe dose, and it is generally controlled by a doctor. After stopping the medicine, the condition can gradually recover.
Irreversible is caused by the body's sensitivity to chloramphenicol or a deficiency in its ability to detoxify. It is not related to the dose, but is related to the genetic abnormality of the individual.
Because this human gene can be changed under the action of chloramphenicol, it causes irreversible and durable changes in bone marrow hematopoietic stem cells, making it unable to differentiate, and as a result, the three lines of bone marrow hematopoietic cells (red, granulocyte, megakaryocyte) are reduced This condition is often severe.
Because chloramphenicol can also be combined with human mitochondrial 70S, it can also inhibit human mitochondrial protein synthesis and cause toxicity to the human body.
Features of Chloramphenicol Test
1. 15min reading results
2. Detection limits respond to EU and US FDA norms
3. Neither professionals nor equipment required
4. Easy to operate and read
Detection Method of Chloramphenicol Test
CAP Test is based on the competitive inhibition immune chromatography assay. During the lateral flow procedure, CAP residues in extracted samples will bind to the colloidal gold-labeled specific monoclonal antibodies, inhibit the combination of antibodies.
Then the CAP-BSA conjugates on the NC membrane, leading to color changes of the test line (line T).
Samples of Chloramphenicol Test
Samples include: pork, chicken, fish, shrimp and other aquatic products.
Detection Limit of Chloramphenicol Test
The LOD may change according to requirement or specimen's difference, please refer to kit instruction for further details.
Importance of Chloramphenicol Test
Chloramphenicol Test can help manufacturers, regulatory agencies, and the public monitor product quality and help humans protect their health.
Components of Chloramphenicol Test
1. Test Cards: 10 pcs
2. Extracting Reagent（6ml）:10 bottle
3. Reagent A（55ml）:1 bottle
4. Reagent B（6ml）:1 bottle
5. Reagent C（12ml）:1 bottle
6. Purifying Reagent（5ml）:2 bottles
7. Derivatization Reagent（2ml）:1 bottle
8. Re-hydration fluid（12ml）:1 bottle
9. Centrifuge Tubes（15ml）:20pcs
10. Product Instruction:1
How to use Chloramphenicol Test?
1. Prepare the sample. (Refer to the instruction.)
2. All reagents and Test Cards should be at room temperature before using. Please use the Test Cards within 1 hour.
3. Using a micropipette, inject 75ml of the sample into the sample hole (S hole).
4. Start the timer when the sample solution flows. After 10mins, interpret the test results according to the sketch picture. The result in other time period is invalid.
Summary
The direct consequence of long-term abuse of antibiotics in aquaculture and animal husbandry is likely to induce antibiotic resistance genes in animals, which will cause potential genetic pollution to the breeding area and its surrounding environment after excretion.
Resistance genes are also very likely to spread in the environment, Proliferation poses a threat to public health and food and drinking water safety.
The chloramphenicol rapid detection kit provided by BALLYA can effectively detect whether chloramphenicol is contained in aquatic products, further ensuring consumer safety. This kit is easy to operate, and has a short test time and high accuracy. The test results comply with EU standards.
How to order Chloramphenicol Test?
Please check the online catalog and contact our sale representative via email: info@ballyabio.com or fill out contact form below:
