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Nitrofurazone Test For Aquatic Products (0.5PPB)


For Aquatic Products

Specs:10 T / box
Testing Time:15 mins
Regulation: CN / EU / RU / US

Operation video

Instrution For Nitrofurazone Test For Aquatic Products (0.5PPB)

SEM Test is based on the competitive inhibition immune Nitrofurazone Metabolite assay. During the lateral flow procedure, SEM residues in extracted samples will bind to the colloidal gold-labeled specific monoclonal antibodies, inhibit the combination of antibodies. Then the SEM-BSA conjugates on the NC membrane, leading to color changes of the test line (line T).

This product is suitable for the qualitative detection of SEM in pork, chicken, fish and shrimp samples.

Limit of Detection
0.5 ng/g (ppb), 1.0 ng/g (ppb)

Test Cards
Extracting Reagent(6ml)
10 bottle
Reagent A(55ml)
1 bottle
Reagent B(6ml)
1 bottle
Reagent C(12ml)
1 bottle
Purifying Reagent(5ml)
2 bottles
Derivatization Reagent(2ml)
1 bottle
Re-hydration fluid(12ml)
1 bottle
Centrifuge Tubes(15ml)
Product Instruction
Storage and Validity
At 4~30℃, out of sun light & moisture, 12 months

This product shows negative result in detecting 500μg/kg of AOZ, SEM, or AHD residues.

Test Procedure
(1) Prepare the sample. (Refer to the instruction.)
(2) All reagents and Test Cards should be at room temperature before using. Please use the Test Cards within 1 hour.
(3) Using a micropipette, inject 75ml of the sample into the sample hole (S hole).
(4) Start the timer when the sample solution flows. After 10mins, interpret the test results according to the sketch picture. The result in other time period is invalid.

Interpretation of Results
Visual interpretation of the result obtained by comparing the intensity of each test line with the control (CTRL) line.
Invalid: Line C does not appear. Please retest with new cards.
Results Illustration
Refer to the instruction

If you want to know more, please contact us

Trivia questions : What is Furacilin and SEM?

What is furacilin?

Bacitracin and polymyxin B are antibiotics that kill bacteria. They are also drugs. They are lemon yellow fine crystalline powders; they are odorless and bitter. It is only used clinically as a disinfectant and antiseptic, and is externally used to treat wounds, burns, purulent dermatitis, otitis media, dacryocystitis, vaginitis, bladder irrigation, bedsores, etc.

Mechanism of action

Furacilin can interfere with the bacterial sugar metabolism process and oxidase system and exert bacteriostatic or bactericidal effects. It mainly interferes with the early stages of bacterial sugar metabolism, leading to bacterial metabolism disorders and death. It has a broad antibacterial spectrum, has antibacterial effects on a variety of Gram-positive and negative bacteria, and also has effects on anaerobic bacteria. It is weak against Pseudomonas aeruginosa and pneumococcus, and is resistant to Pseudomonas and Proteus It is not effective against fungi and molds, but it is still quite effective against bacterial infections caused by molds. The sterilization concentration for sensitive bacteria is 13 ~ 20ug / ml, and the antibacterial concentration is 5 ~ 10ug / ml.

Furacilin uses

Furacin ointment

Furacilin cream is indicated for mild purulent skin diseases.
1. Should not be used on damaged skin.
2. Avoid contact with eyes and other mucous membranes (such as mouth, nose, etc.).
3. If there is a burning sensation, itching, redness or swelling in the application site, the drug should be discontinued, and the local drug should be washed. If necessary, consult a physician.
4. For those who are allergic to this product, use it with caution.
5. It is forbidden to use this product when its properties are changed.
6. Keep this product out of the reach of children.
7. Children must use under adult supervision.
8. If you are using other medicines, please consult your physician or pharmacist before using this product.

Furacilin solution

Topical antibacterials. Can interfere with the bacterial oxidase system and exert antibacterial or bactericidal effects. It is used for ear, nose and skin diseases caused by various Gram-positive and negative bacteria. It is also effective for infections caused by anaerobic bacteria. Furacilin solution has disinfection and antiseptic effect. It is mainly used for disinfection of skin, mucous membrane and cavity in clinic.
Note: This product can cause allergic reactions, such as allergic dermatitis.

Furacilin dosage

Topical / Instant
Wounds, burns, ulcers and skin infections; prepare the surface before skin grafting
Adult: Applied to the affected area in a 0.2% topical formulation on a water-soluble or water-miscible basis.

Furacilin side effects

Topical application can occasionally cause skin allergic reactions. Furacilin can interfere with the bacterial sugar metabolism process and oxidase system, and exert bacteriostatic and bactericidal effects. It mainly interferes with the early stages of bacterial sugar metabolism, leading to bacterial metabolism disorders and death. The spectrum is relatively wide, it has antibacterial effects on a variety of Gram-positive and negative bureaus, and it also has effects on anaerobic bacteria. Pseudomonas aeruginosa and pneumococcus are less active.

Furacilin allergy

For patients with drug allergies, it can cause allergic reactions after the application of Furacilin. For example, oral administration can even cause shock, asthma, tightness, rashes, etc. Occasionally there are some gastrointestinal reactions, drug fever, eosinophilia, and neurological symptoms, such as hallucinations, hallucinations, hallucinations, dizziness Insomnia and multiple peripheral neuritis.

About SEM

SEM is a metabolite of Furacilin, because Furacilin is a nitrofuran drug, and the prototype nitrofuran drug is metabolized rapidly in the body, and its metabolites are SEM, which is relatively stable in combination with protein, so it is often used to detect metabolites. Residual status of nitrofurans.

Furacilin in the veterinary

Furacilin is a synthetic antibacterial drug that treats livestock diseases. A few years ago, it was widely used in animal husbandry and aquaculture. Subsequent research found that furancillin, furazolidone, and furancilinone residues and their metabolites in foods of animal origin can be transmitted to humans through the food chain. Entry can cause a variety of diseases and cause carcinogenic and teratogenic effects on the human body. The United States, Australia, Canada, Japan, Singapore and the European Union have clearly stipulated that the use of such drugs is strictly prohibited in the food industry, and strict inspection of nitrofuran residues in aquatic products is strictly enforced. Furacilin is listed as a banned drug in China.


The residues of "furancilin, furazolidone" and its metabolites in nitrofuran drugs in animal-derived foods can be transmitted to humans through the food chain. Long-term ingestion can cause various diseases and have carcinogenic and teratogenic side effects on the human body. Although it has been banned for use in the aquaculture industry, some unscrupulous businesses have taken risks and know the law.
The Nitrofurazone Test For Aquatic Products kit provided by BALLYA  can effectively detect whether aquatic products contain furancilin and ensure consumer food safety. This kit is easy to operate, and has a short test time and high accuracy.

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