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Furazolidone Test For Aquatic Products (0.5PPB)


For Aquatic Products

Specs:10 T / box
Testing Time:15 mins
Regulation: CN / EU / RU / US

Operation video

Instrution For Furazolidone Test For Aquatic Products (0.5PPB)

AOZ Test is based on the competitive inhibition immune chromatography assay. During the lateral flow procedure, AOZ residues in extracted samples will bind to the colloidal gold-labeled specific monoclonal antibodies, inhibit the combination of antibodies. Then the AOZ-BSA conjugates on the NC membrane, leading to color changes of the test line (line T).

This product is suitable for the qualitative detection of AOZ in pork, chicken, fish and shrimp samples.

Limit of Detection
0.5 ng/g (ppb), 1.0 ng/g (ppb)

Test Kit
Extracting Reagent(6ml)
10 bottle
Reagent A(55ml)
1 bottle
Reagent B(6ml)
1 bottle
Reagent C(12ml)
1 bottle
Purifying Reagent(5ml)
2 bottles
Derivatization Reagent(2ml)
1 bottle
Re-hydration fluid(12ml)
1 bottle
Centrifuge Tubes(15ml)
Product Instruction
Storage & Shelf-Life
At 4~30℃, out of sun light & moisture, 12 months

This product shows negative result in detecting 500μg/kg of AOZ, SEM, or AHD residues.

Test Procedure
(1) Prepare the sample. (Refer to the instruction.)
(2) All reagents and Test Cards should be at room temperature before using. Please use the Test Cards within 1 hour.
(3) Using a micropipette, inject 75ml of the sample into the sample hole (S hole).
(4) Start the timer when the sample solution flows. After 10mins, interpret the test results according to the sketch picture. The result in other time period is invalid.

Interpretation of Results 
Visual interpretation of the result obtained by comparing the intensity of each test line with the control (CTRL) line.
Invalid: Line C does not appear. Please retest with new cards.

Results Illustration

Refer to the instruction

If you want to know more, please contact us

Trivia questions : What is Furazolidone and AOZ?

About furazolidone

Furazolidone synthesis

1. Ethanolamine is used as a raw material to condense with urea to obtain β-hydroxyethyl urea, which is nitrosated and cyclized to obtain 3-nitroso-2-azolidinone. Further reduction with iron powder and condensation with 5-nitro-2-furfuryl glycol diacetate and formaldehyde yield furazolidone.
2. Condensation of ethanolamine with urea, nitrosation and cyclization with sodium nitrite, and finally reduction with iron powder with 5-nitro-2-furfuryl glycol diacetate and formaldehyde to obtain furazolidone.

Furazolidone brand name

Furazolidone is a nitrofuran antibacterial agent and a monoamine oxidase inhibitor (MAOI). It is sold by the Roberts Laboratories under the trade name Furoxone and by GlaxoSmithKline as Dependal-M.
Brand Name       Composition                                                                           Company
furoxone tab       Furazolidone 100 mg                                                            glaxo smithkline
furoxone susp     Furazolidone 25 mg/5ml                                                     glaxo smithkline
megyl-f syrup     Furazolidone 30 mg, metronidazole 100 mg/5ml        indoco

Furazolidone mechanism of action

Antibacterial effect on Gram-positive and negative bacteria, including Salmonella, Shigella, E. coli, Klebsiella pneumoniae, Enterobacter, Staphylococcus aureus, Enterococcus faecalis, Streptococcus pyogenes, Cholera Vibrio, Campylobacter and Bacteroides are also active against Trichomonas and Giardia under certain concentrations. Its mechanism of action is to interfere with bacterial oxidoreductase and thereby block the normal metabolism of bacteria.

Furazolidone uses

Furazolidone spray

Furazolidone spray is specifically for the treatment of horses, for topical application in horses and ponies. For the prevention or treatment of superficial wounds, abrasions and lacerations caused by Staphylococcus aureus and Streptococcus. And Proteus. Sensitive to furazolidone.
Not suitable for animals intended for food. Use only as recommended by a veterinarian to treat puncture wounds, wounds that require surgical debridement or sutures, pride of meat, systemic and chronic infections of the skin, and chronic wounds of skin conditions associated with intense itching. If redness, inflammation or swelling persists or worsens, discontinue use and consult a veterinarian.
Wear gloves when washing or wash hands afterwards. Avoid contact with eyes.

Furazolidone veterinary

When used as a veterinary drug, furazolidone has a good effect on the prevention and treatment of certain protozoan diseases, water mold diseases, bacterial rotten gills, erythroderma, and bleeding diseases.
In the breeding industry, furazolidone can be used to treat intestinal infections in livestock and poultry, such as piglet yellow and white pheasant. In the aquaculture industry, furazolidone has a certain effect on the infection of cerebral myxosoma by Salmonella.
Furazolidone is listed by the Ministry of Agriculture of the People's Republic of China as a banned drug and cannot be detected in animal foods.

Furazolidone for dogs

It is usually used to treat intestinal infections and coccidiosis in dogs, and it is effective against E. coli and dysentery.

Furazolidone for cats

For cat intestinal infections, coccidiosis, giardiasis have significant effects.

Furazolidone for fish

Furazolidone is the main component of tetriline, an ornamental fish medicine "yellow powder", which can interfere with the bacterial sugar metabolism process and oxidase system and exert bacteriostatic or bactericidal effects. So it can be used to treat bacterial diseases of ornamental fish disease. And the effect is very good.

Furazolidone side effects

Mainly nausea, vomiting, diarrhea, headache, dizziness, drug fever, rash, anal itching, asthma, orthostatic hypotension, hypoglycemia, pulmonary infiltration, etc. Occasionally hemolytic anemia, jaundice and polyneuritis may occur.

Toxic side effect

Excessive use of furazolidone may cause gastrointestinal reactions (such as nausea, vomiting, anorexia, diarrhea, and generally mild reactions); allergic reactions such as hemolytic anemia, rash, drug fever; polyneuritis; newborns and G-6- PH deficiency can cause hemolytic anemia.
Overdose or long-term continuous medication can cause animal poisoning and severely cause animal death. It can cause irreversible damage to the central nervous system, and can cause diseases such as hemolytic liver necrosis in the human body. As early as 2002, the Ministry of Agriculture listed it as a banned veterinary drug and it is strictly prohibited to use it in any food animal. However, in order to pursue economic benefits, some farmers still use it as a special medicine for treating aquatic gastrointestinal diseases.
Studies have also shown that furazolidone for long or high doses has varying degrees of toxic and side effects on liver, kidney, heart, hypothalamus and reproductive systems of animals.

Furazolidone interaction

(1) Combination with tricyclic antidepressants can cause acute toxic psychosis and should be avoided.
(2) This product can enhance the effect of levodopa.
(3) Sympathomimetic amines, tyramine-rich foods, appetite suppressants, monoamine oxidase inhibitors, etc. can enhance the effect of this product.

About AOZ

The detection of furazolidone must be based on the detection of a series of metabolites.
Therefore, these metabolites AOZ are used as detection targets for abused furazolidone drugs.


Veterinary drugs play a very important role in the modern breeding industry, but in fact, some breeders or enterprises in order to save costs and obtain higher antibacterial effects, regardless of national regulations, repeatedly use antibacterial drugs or even use alternative veterinary drugs, resulting in animal origin Foods Although hydrogen nitrate is a broad-spectrum antibacterial drug, it has a bactericidal effect on most Gram-positive bacteria and Gram-negative bacteria, but research has found that these drugs are carcinogenic, mutagenic and teratogenic. Therefore, countries have listed it as an alternative veterinary drug.
The Furazolidone Test For Aquatic Products (0.5ppb) provided by BALLYA can effectively detect whether furazolidone is contained in the test object, ensuring the safety of consumers. The operation of the kit is very simple, and the detection time is short, the accuracy is high, and the test results comply with EU standards.

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