ballya-test-kitBALLYA PAT bar Test

What is BALLYA PAT bar Test?

BALLYA PAT bar Test is manufactured by BALLYA, it’s rapid test for detection of the genetically modified of PAT bar protein in grains, food and edible oil, especially in grain and food. Lateral flow assay test base on the gold immunochromatography assay technology.
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The detection principle of BALLYA PAT bar Test

This kit is specifically designed for the PAT bar protein expressed by the bar/pat gene in transgenic plants, and is developed by using monoclonal antibody preparation, immunochromatography and colloidal gold tracing techniques.

Compared with traditional PCR and West blot detection methods, it has many characteristics such as simplicity, rapidity, accuracy and high sensitivity. It can directly qualitatively identify the presence or absence of bar/pat protein in genetically modified plants and compare it with other genetically modified materials or foreign proteins. No cross-reaction and high specificity.

How to use the BALLYA PAT bar Test?

Sample processing

Genetically modified seeds:
1. Weigh 20-100 mg of genetically modified materials (seeds, leaves, callus, etc.), fully crush them, put them in a collection tube, and add 1 mL of extract.

2. Shake up and down for 10 sec, and centrifuge at 12,000×g for 2 min at room temperature.

3. Carefully aspirate the supernatant and transfer it to a new collection tube.

Transgenic leaves and callus:
1. Put 20-100 mg of genetically modified material in the collection tube and add 1 mL of extract.

2. Grind the genetically modified material with a grinding rod, and centrifuge at 12,000×g for 2 min at room temperature.

3. Carefully aspirate the supernatant and transfer it to a new collection tube.

Inspection process

1. Take out the test paper packaging tube and return it to room temperature. After taking out the test paper that needs to be used, immediately reclose the packaging tube and put it back. Do not bend the test paper.

2. Keep the reaction tube upright and insert the test paper into the reaction tube. The sample liquid will rise along the test paper.

3. Read the result after 10 minutes of reaction. A positive result may be judged in a shorter time.

4. If you need to save the test paper, discard the end part marked with an arrow.

Read the result of BALLYA PAT bar Test

The color of the control line within 10 minutes indicates that the test paper is effective. The test paper that does not show color on the control line is a failure product. Please use a new test paper for testing.

If the test sample contains PAT/bar protein, the test line will be colored. This result was judged to be a positive result. If the test sample is negative, only the Control Line is displayed on the test paper.

Precautions for BALLYA PAT bar Test

1. Keep the outer packaging intact to prevent the test strip from getting wet.

2. Each time a new sample is ground, the grinding rod must be replaced. Because the test strip is very sensitive, it is easy to cause cross-contamination and cause false positives.

3. After the plant material is ground, it should be centrifuged sufficiently, otherwise it will affect the liquid rising speed and detection effect.

4. During the test, the liquid must not exceed the Max line along the bottom of the test strip, otherwise it will affect the test results.

5. If the quality inspection line of the test strip does not appear after 2-3 minutes, please replace the test strip and contact your local dealer in time.
Note: The above content is for reference only! Please learn more under the guidance of experts!

What specimen can be detected by BALLYA PAT bar Test?

Specimen include: all kinds of grains, food and edible oil. Widely used in feed industry and food processing industry.
You can choose the right products according to your needs.

Significance of testing PAT bar

To protect the animal have health grain, food, and edible oil, avoid contaminated food. The excess genetically modified of PAT bar genes is harmful for animal being, can’t take too much in the body.

Component of BALLYA PAT bar Test?

BALLYA PAT bar Test, 25 cassettes
Sample Buffer, 1 vial
Mini Pipette (optional)
Disposable micropipette tips (optional)
Kit Instruction, 1pcs
PAT bar Test

How to use BALLYA PAT bar Test?

1. Take some grain or food specimen
2. Add the supernatant to cassette
3. Wait for 15 minutes to result
4. The details of operation, refer to the kit instruction

Limitations of BALLYA PAT bar Test?

BALLYA PAT bar Test is a qualitative test kit. It’s only for screening purpose. If have positive cases or suspected case, maybe use other detection method to make a further detection, such as, ELISA, PCR, qPCR, etc.


BALLYA PAT bar Test is suitable for the detection of PAT bar genes in grain, food and edible oil. Simple operation, high accuracy and short detection time are the characteristics of this product.

It can be widely used in food and drug administration, health departments, higher education institutions, scientific research institutes, agricultural departments, animal husbandry and veterinarians, breeding farms, slaughterhouses, food and meat products deep processing enterprises, inspection and quarantine departments and other units.

Where to buy BALLYA PAT bar Test?

Precautions for BALLYA PAT bar Test

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