BALLYA Danofloxacin Test is manufactured by BALLYA, it’s rapid test for detection Danofloxacin residues in egg, milk & pork product, especially in pork. Lateral flow assay test base on the gold immunochromatography assay technology.
Danofloxacin is a kind of fluoroquinolone medicine, which belongs to animal special drugs. It is generally used as its mesylate salt, danofloxacin mesylate. All have strong antibacterial activity. Excessive residues of quinolones are likely to induce bacterial resistance and cause harm to human health. The EU's maximum residue limit for fluoroquinolones including danofloxacin in food animals is: muscle 100μg / kg, Liver and kidney 200μg / kg, poultry and pig edible tissue 50μg / kg.
Danofloxacin mechanism of action
The drug concentration in lung tissue can reach 5-7 times of plasma. Oral administration, intramuscular injection and subcutaneous injection are more rapid and complete. The bioavailability of chicken oral administration is 100%; the bioavailability of pig oral administration is 89%; the bioavailability of cattle oral administration is 72%. Excreted mainly through the kidneys. Danofloxacin mesylate is rapidly absorbed and distributed in crucian carp, and eliminated slowly; the drug has strong penetration and wide distribution in tissues, and the drug concentration in tissues is significantly higher than that in plasma from 12h to 168h (P <0.05). The hepatopancreas and kidneys had the highest drug concentration in all tissues. The elimination half-lives of kidney, hepatopancreas, plasma, muscle and skin were 33h, 44h, 48h, 51h and 177h, respectively. After 120 hours of administration, the drug concentration in the skin was 1.99 ± 0.32 μg / g, after 168 hours of administration, the drug concentration in the skin was 1.72 ± 0.31 μg / g, and in muscle was 0.70 ± 0.32 μg / g, compared with other tissues in the skin The elimination of the drug is the slowest, and it is recommended as the residual accumulation tissue of DFM in crucian carp.
Using trans-4-hydroxy-L-proline as the raw material, it is obtained by amino protection, carboxyl reduction, hydroxysulfonylation, ring closure, and deprotection to obtain (15, s4) -2-methyl-2,5 -Diazabicyclo [2.2.1] heptane hydrobromide, which is then reacted with cyclopropanecarboxylic acid to produce danofloxacin.
How Danofloxacin uses for?
This product is a broad-spectrum bactericide. It has strong antibacterial activity against Pasteurella, Mycoplasma and Escherichia coli. Mainly used for avian colibacillosis, avian cholera, chronic respiratory disease, etc., to treat diseases caused by animal bacteria and mycoplasma in cattle, pigs, and chickens.
Intramuscular injection, 1 day, danofloxacin mesylate per kg body weight, livestock 10-20 mg, poultry 20-40 mg, divided into two injections. Mixed drink: poultry 15-30 mg per liter of water.
What's Danofloxacin side effect?
Dorfloxacin was orally irrigated with Sturgeon at doses of 800, 1040, 1352, 1757.60, 2248.88, 2970.34 mg / kg, and intraperitoneally injected with 400, 440, 532.4, 585.6, and 644.2 mg / kg. Observe the behavior and death of the experimental fish for 10 consecutive days. The LD50 of darofloxacin injected intraperitoneally by Shi's sturgeon was 429.47 mg / kg, and the LD50 of dalofloxacin by oral infusion was 1502.10 mg / kg. The liver cells of the dead fish were filled with lipid droplets, the mitochondria were broken, the rough endoplasmic reticulum structure was loose and broken, and the lysosome was broken.
What is Danofloxacin mesylate?
Danofloxacin mesylate mainly has a strong antibacterial effect on Gram-positive bacteria (G +), certain anaerobic bacteria and mycoplasma. Sensitive to Staphylococcus aureus, Streptococcus hemolyticus, Pneumococcus, Legionella pneumophila, Mycoplasma, also sensitive to Clostridium, Clostridium welchii, Tetanus, and Bacteroides. Danofloxacin mesylate is clinically used for infections caused by epirubicin and erythromycin; Mycoplasma infection causes chronic respiratory disease and swine asthma in chickens; Necrotizing enteritis in chickens; Treponema pallidum dysentery and toxoplasmosis; Actinomycosis. The quinolone antibacterial mechanism is generally considered to specifically inhibit DNA helicase, acting on the A subunit of DNA helicase, inhibiting the function of helicase, blocking the semi-retained replication of bacterial DNA, causing irreversible damage to chromosomes, and bacteria no longer dividing Therefore, the antibacterial effect is more thorough than that of sulfonamides and antibiotics.
Danofloxacin mesylate solubility
Danofloxacin mesylate is white to light yellow crystalline powder, odorless, bitter, easily soluble in water, slightly soluble in methanol, almost insoluble in chloroform.
Danofloxacin mesylate injection
Reduce the production of drug-resistant bacteria and ensure the effectiveness of levofloxacin mesylate and other antibacterial drugs. Levofloxacin is only used to treat or prevent infections that have been or are highly suspected to be caused by sensitive bacteria. When selecting or modifying antimicrobial treatment regimens, the results of bacterial culture and drug susceptibility testing should be considered.
What specimen can be detected by BALLYA Danofloxacin Test?
Specimen include: pork, chicken, milk, etc. Widely used in pork industry and dairy industry.
Significance of testing Danofloxacin
To protect human have health food and avoid contaminated food. The Danofloxacin is harmful for human being, can not take too much in body.
1. Take pork specimen and extrat supernatant 2. Add the supernatant to cassette 3. Wait for 15 minutes to result 4. The details of operation, refer to the kit instruction
Limitations of BALLYA Danofloxacin Test?
BALLYA Danofloxacin Test is a qualitative test kit. It’s only for screening purpose. If have positive cases or suspected case, maybe use other detection method to make a further detection, such as, ELISA, PCR, qPCR, etc.
Danofloxacin has been widely used as an anabolic hormone in various industries. The pig breeding industry deserves special attention. Pork provides people with rich nutrition, making it indispensable in daily life. The problem of danfloxacin hormone residues in pork has been plagued by people, so it is necessary to detect pork in time. BALLYA Danofloxacin Test provided by BALLYA can effectively and quickly detect the presence of danfloxacin residues in pork. Not only the operation is simple, the test time is short, but the tester can get the test result in the first time.