BALLYA Vibrio Parahaemolyticus Test is manufactured by BALLYA, it’s rapid test for detection the content of vibrio parahaemolyticus in feed, food, seafood and medicine, especially in feed and food. Lateral flow assay test base on the gold immunochromatography assay technology.
Vibrio parahaemolyticus, also known as Vibrio enteritidis, belongs to the genus Vibrio and is a common pathogen. It is a gram-negative bacillus with arc-shaped, rod-shaped, filamentous and other shapes without spores.
Biological characteristics of Vibrio parahaemolyticus
Vibrio parahaemolyticus is a halophilic bacterium, a facultative anaerobe, a polymorphic bacillus or a slight Campylobacter. This fungus is more sensitive to acid, and cannot grow when the pH is below 6, and will die in 1 to 3 minutes in ordinary vinegar.
Low resistance to high temperature, 50 ℃ 20 min; 65 ℃ 5 min or 80 ℃ 1 min can be killed. These bacteria have weak resistance to common disinfectants and can be killed by low-concentration phenol and cresol soap solutions.
What is Vibrio Parahaemolyticus enteritis?
Pathogenesis of Vibrio parahaemolyticus enteritis
The disease can be caused by swallowing more than 100,000 live bacteria, and some may show sepsis.
The bacterium has an aggressive effect. The antigenicity and immunity of TDH and TRH produced by it are similar. Both have hemolytic activity and enterotoxin effect, which can cause intestinal loop swelling, congestion and intestinal fluid retention, causing diarrhea.
TDH has specific cardiotoxicity to the heart and can cause atrial fibrillation, premature contraction or myocardial damage. Some people have found that urease disease is related to diarrhea.
Distribution and transmission route of Vibrio parahaemolyticus enteritis
It is widely distributed, mainly in seawater and aquatic products. The infections of mountainous and inland residents go to coastal areas and the infection is more serious. The detection rate of Vibrio parahaemolyticus in the seawater along the coast of East China is 47.5%~66.5%. The average carrier rate of marine fish and shrimp is 45.6%~48.7%, and can be as high as 90% in summer.
In addition to seafood, Vibrio parahaemolyticus was found in livestock and poultry meat, pickled vegetables, salted eggs, and freshwater fish.
Seawater is the source of pollution for these bacteria. Seafood, sea salt, carriers, etc. may become a way to spread these bacteria. In addition, residents and fishermen with a history of intestinal diseases have a high rate of carrying bacteria and are also a source of infection.
Clinical manifestations of Vibrio parahaemolyticus enteritis
The incubation period ranges from 1 hour to 4 days, most of which are about 10 hours. The onset is sudden, often with abdominal pain, diarrhea, vomiting, dehydration, chills and fever. Abdominal pain is mostly chronic colic, often located in the upper abdomen, umbilical cord or ileocecal area.
What are the Vibrio Parahaemolyticus harm and prevention?
Complications of Vibrio parahaemolyticus
1. It may be complicated by toxic shock: the patient may show that blood pressure drops, heart rate increases, palpitation, palpitations, etc. are not symptoms.
2. Severe diarrhea can lead to dehydration, electrolyte imbalance, and circulatory failure: Because a large amount of fluid is lost through the digestive tract, if sufficient fluid is not replenished in time, it is easy to cause water and electrolyte imbalance, leading to circulatory failure.
3. Consciousness disorder: If toxic food exists in the body for a long time, it may enter the blood supply of the brain through the circulation, causing consciousness disorder and even brain death.
Treatment measures of Vibrio parahaemolyticus
1. Support and symptomatic treatment Dehydrated patients need to enter normal saline and grape saline, or oral rehydration salts to correct dehydration. In patients with lowered blood pressure, in addition to passively supplementing blood volume and correcting acidosis, vasoactive drugs can be used as appropriate.
2. Antibacterial drugs Mild patients may not need antibacterial drugs, and heavier patients can be given compound trimethoprim or gentamicin, amikacin and norfloxacin and other quinolone antibacterial drugs. [The above antibiotics may cause bacteria to develop resistance, so use caution].
3. Stop eating suspiciously poisoned food immediately after poisoning and go to the hospital for treatment. Vibrio parahaemolyticus is sensitive to chloramphenicol. People with severe vomiting and diarrhea should add water and salt.
Preventive measures of Vibrio parahaemolyticus
1. Strengthen the sanitary treatment of seafood. The cleaning, salting, refrigeration, and transportation of seafood should be managed strictly in accordance with hygiene regulations.
2. Prevent cross-contamination of raw and cooked food, and do not eat raw seafood. Separate lettuce and cooked vegetables to prevent cross infection. The seafood should be cooked thoroughly. Stored food should be re-cooked before eating. Don't eat wedelia, raw swimming crabs, salted grilled shrimps, etc. If eaten raw, soak in vinegar for 5 minutes to kill pathogenic bacteria.
3. Control the growth of bacteria in food. Usually food should be placed in a cool and ventilated place, or kept in the refrigerator. The leftovers between meals should be fully heated before eating. It should not be left at room temperature for too long.
Note: The above content is for reference only! If you have similar symptoms, seek medical attention immediately!
What specimen can be detected by BALLYA Vibrio Parahaemolyticus Test?
Specimen include: all kinds of feed, food, seafood and medicine. Widely used in feed industry and food processing industry. You can choose the right products according to your needs.
Significance of testing Vibrio Parahaemolyticus
To protect the animal have health feed, food, seafood and medicine, avoid contaminated food. The excess vibrio parahaemolyticus is harmful for animal being, can’t take too much in the body.
1. Take some feed or food specimen 2. Add the supernatant to cassette 3. Wait for 15 minutes to result 4. The details of operation, refer to the kit instruction
Limitations of BALLYA Vibrio Parahaemolyticus Test?
BALLYA Vibrio Parahaemolyticus Test is a qualitative test kit. It’s only for screening purpose. If have positive cases or suspected case, maybe use other detection method to make a further detection, such as, ELISA, PCR, qPCR, etc.
BALLYA Vibrio Parahaemolyticus Test is suitable for the detection of microorganism residues, toxic and harmful substances and antibiotic residues in feed, food and medicine.
It can be widely used in food and drug administration, health departments, higher education institutions, scientific research institutes, agricultural departments, animal husbandry and veterinarians, breeding farms, slaughterhouses, food and meat products deep processing enterprises, inspection and quarantine departments and other units.