Chloramphenicol Test

Chloramphenicol Test is based on the Colloidal Gold Immunochromatography to detect the Chloramphenicol residues in honey products. This kit can be applied for on-site rapid or lab testing by various departments.

Chloramphenicol is a broad-spectrum bacteriostatic agent. It can be diffused into the bacterial cells through fat solubility, mainly acting on the 50s subunit of the bacterial 70s ribosome, inhibiting transpeptidase, inhibiting the growth of the peptide chain, inhibiting the formation of the peptide chain, thereby preventing protein synthesis. Bacteria that are highly sensitive or highly sensitive to this product also have a bactericidal effect.

Synthesis of p-nitroacetophenone as raw material, bromination to form p-nitro-α-bromoacetophenone, salt formation with cyclohexamethylenetetramine, hydrolysis with hydrochloric acid to obtain p-nitro-α-aminobenzene Ethyl ketone hydrochloride, acetylated with acetic anhydride, and then condensed with formaldehyde, hydroxymethylated to give p-nitro-α-acetylamino-β-hydroxyphenylacetone, reduced with aluminum isopropoxide to obtain (±) sulose Type-1-p-nitrophenyl-2-acetylaminopropanediol, hydrolyzed to remove acetyl group by hydrochloric acid, and neutralized with alkali to obtain (±) sulose type-1-p-nitrophenyl-2-aminopropanediol (amino Material), and the crystallization method was used to resolve it to obtain the D (-)-suara sugar type amino compound, which was finally obtained by dichloroacetylation.

A. Typhoid fever and other Salmonella infections;

B. Ampicillin-resistant Haemophilus influenzae type B meningitis or Streptococcus pneumoniae, Neisseria meningitidis meningitis, sensitive Gram-negative meningitis in patients allergic to penicillin.

C. Brain abscesses, especially otogenic, are often mixed infections of aerobic and anaerobic bacteria.

D. Severe anaerobic infections;

E. No other low-toxic antibacterial drugs can be used to treat various serious infections caused by sensitive bacteria;

F. Rickettsia infection can be used for the treatment of Q fever, Rocky Mountain spotted fever, endemic typhus and so on.

A. Chloramphenicol for dogs

If your dog suffers from conjunctivitis and keratitis, chloramphenicol eye drops are a good choice: its price is cheap, and dogs without eye diseases can also use it to rinse their eyes and keep them clean.

B. Chloramphenicol for cats

Paratyphoid fever, also known as salmonellosis, is an infectious disease of young cats. Commonly used: chloramphenicol subcutaneously or intramuscularly, dosage 20 mg a thousand body weight, once a day, continuous use for 3-5 days.

Chloramphenicol eye drops can be used to wash eyes. It can effectively solve the problem of cat tears when the eyes are red or the eyes are increased.

A. Oral: 0.5g for adults, once every 6 hours. A larger first dose can be used for the treatment of typhoid fever. The patient should continue to apply for 2-3 days after the body temperature is normal to prevent recurrence. The maximum dose is not more than 26g. Children per day 25 kg of body weight ~ 50 mg, divided dose once every 6 hours. Intramuscular injection or intravenous drip: 1day volume: 0.5-1g, divided into 2 injections. Dilute with liquid, 1 chloramphenicol (250 mg) with at least 100ml of diluent.

B. Eye drops: 1-2 drops each time, 3-5 times a day. Ear drops: 2-3 drops each time, 3 times a day. Note: Chloramphenicol injection (containing ethanol, glycerin or propylene glycol and other solvents), It should be extracted with a dry syringe and shaken while diluting to prevent precipitation of crystals.

C. After the symptoms subside, the dosage should be reduced or discontinued as appropriate.

The main adverse reaction is to suppress bone marrow hematopoietic function. There are two symptoms:

One is the reversible reduction of various types of blood cells, in which the granulocytes decline first. This response is related to the dose and course of treatment. Once found, the drug should be stopped in time and can be recovered;

Second, irreversible aplastic anemia, although rare, has a high mortality rate. This reaction belongs to allergy and has no direct relationship with the dosage course. It may be related to chloramphenicol inhibiting the same 70S ribosome in bacteria in mitochondria of bone marrow hematopoietic cells.

Chloramphenicol (levosycin) is a broad-spectrum antibacterial drug that has a stronger effect on gram-negative bacilli than gram-positive bacteria; it also has antimicrobial effects on anaerobic bacteria, chlamydia, mycoplasma and rickettsiae. Oral absorption is rapid and complete, plasma protein binding rate is about 60%, half-life is 1.5-3.5h, and volume distribution is 0.92L / kg. It Can enter the cerebrospinal fluid through the blood cerebrospinal fluid barrier, can also enter the fetal circulation through the blood placental barrier, it can still be distributed to milk, saliva, chest, ascites and synovial fluid. 

The most suitable therapeutic drug concentration range is 5 ~ 20μg / ml, beyond this range can increase the risk of bone marrow suppression. The usual dose for adults is l ~ 2g / d, taken in 3 ~ 4 times; or intravenous infusion after dilution, 1 ~ 2g / d, given in 2 times. The dosage should be adjusted when applying this medicine when liver and kidney dysfunction.

1. 15min reading results

2. Detection limits respond to EU and US FDA norms

3. Neither professionals nor equipment required

4. Easy to operate and read

The Chloramphenicol Test, based on colloidal gold competition method, is used for testing Chloramphenicol residues in honey sample and meets EU MRL. This kit can be applied for on-site testing by various departments

Samples include: honey

The LOD may change according to requirement or specimen's difference, please refer to kit instruction for further details.

Chloramphenicol Test can help manufacturers, regulatory agencies, and the public monitor product quality and help humans protect their health.

1. Test Kit: 10pcs

2. Hexane（12ml）:1bottle

3. Product Instruction:1

4. Micro well plate: 10pcsc

5. Re-Hydration Fluid（2ml）: 1bottle

6. Centrifuge Tube (15ml): 20pcs

1. Ethyl Acetate

2. Mixer

3. Oscillator

4. Electronic Balance

5. Centrifuge

6. Organomation

7. Mini Pipette

1. Read the instruction and let the sample at room temperature （20-25℃）.

2. Take out the test strip, use within 1h.

3. Drop 100µL of sample into the micro well, pipetting up and down for 6 times to dissolve the reagent. Then, incubate for 5mins at room temperature.

4. Drop 100µL sample into the sample well, stand for 5mins at room temperature.

5. Read the result within 5-8mins. The result after 5-8mins is invalid.

When antibiotics were first used on humans, we regarded them as "elixir". As the problem of antibiotic abuse continues to worsen, their resistance has also brought huge harm to humans.

Therefore, in daily life, we should avoid food with antibiotic residues. The first step to self-help: we need to detect antibiotic residues.

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