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BALLYA Brucellosis Test

Ballya-antibiotic-testBALLYA Brucellosis Test

What is BALLYA Brucellosis Test?

BALLYA Brucellosis Test is manufactured by BALLYA, it’s rapid test for detection Brucellosis disease in ruminant and pets. Lateral flow assay test base on the gold immunochromatography assay technology.
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What is Brucellosis?

Is brucellosis zoonotic?

Yes, it is. Brucella can be housed in animals such as sheep, goats, pigs, cattle and dogs. Humans are usually infected by contact with the secretions of infected animals or by eating contaminated meat or dairy products. Infection is rare. The symptoms are similar to the flu.

Brucellosis symptoms

Patients often present with relaxing fever, fatigue, night sweats, loss of appetite, and anemia. In some cases, there are infections of the lungs, gastrointestinal tract, subcutaneous tissue, testes, epididymis, ovaries, gallbladder, kidneys and brain. May be accompanied by hepatosplenic lymphadenopathy, multiple, migrating systemic muscle and large joint pain, which later manifested as skeletal involvement, with spine involvement being the most common, especially lumbar spine.

Brucellosis diagnosis

Generally, fever is about one month after the acute phase, and diagnosis and treatment are very important at this time. After a blood test confirms the diagnosis, patients usually need to take antibiotics for at least 3 weeks. Even if the symptoms disappear during this period, you cannot easily “give up treatment”, otherwise it will be more difficult to cope with the relapse. If treated promptly, the recovery time in most cases is weeks to months. If the "combatant" is mistaken for a chronic disease, it will face a long and repetitive course and unbearable pain, and complications such as arthritis may also occur. The most serious are inability to work, female miscarriage or infertility.

What is Brucella?

Brucella is a gram-negative, non-moving bacterium, without capsules (smooth microcapsules), positive for enzymes and oxidases, absolutely aerobic bacteria, can reduce nitrate, intracellular parasitism, can survive in a wide variety of domestic animals.

Brucella species

In 1985, the World Health Organization (WHO) Brucellosis Expert Committee divided Brucella into six species and 19 types, namely sheep species Brucella (3 types), cattle species Brucella Bacillus (type 8), Brucella suis (type 5), Brucella sheep epididymis (type 1), Brucella species (1 type) in the desert forest and wild dog species Bacillus (1 type). Diseases of humans are pigs, cattle, sheep, and dogs, which can directly infect humans through diet and injured skin and mucous membranes. Of these, Brucella, the "first show" in Malta, has the strongest pathogenicity

Brucella antibody

Laboratory significance of Brucella antibodies:
Elevation: Agglutination price> 1:80 may be brucellosis, with a positive rate of 90% to 94%. Repeated examinations with increasing agglutination value are more diagnostic. Chinese scholars have observed that after injection of cholera vaccine, the agglutination price of brucella in some patients' serum was very high.

Which animals will suffer from brucellosis?

Brucellosis in dogs

Brucellosis in dogs is a zoonotic disease caused by brucella. After being infected, most dogs are recessive and lack obvious clinical symptoms.

Brucellosis symptoms in dogs

Symptoms after infection in male dogs: orchitis, prostatitis, penile inflammation, epididymitis, testicular atrophy, arthritis, claudication, tenosynovitis, etc. Symptoms of bitches after infection include abortion (about 45 days), vaginitis, and increased vaginal discharge.

Brucellosis in cattle

Both bulls and cows are susceptible to this pathogen. Cattle with this disease are the main source of infection for bovine brucellosis. When a diseased cow has an abortion, numerous pathogens are excreted with vaginal secretions, posing a great threat to surrounding cows that have not yet been infected. Vulnerable cattle may have eaten feed contaminated with brucella, bedding, or licking contaminated utensils with their tongues.

Brucellosis in goats

Goats brucellosis is a chronic infectious disease in sheep. Mainly affects the reproductive system. Sheep infections are characterized by abortions in the ewe and orchitis in the ram.

How to treat Brucellosis?

Drug therapy can be started after a clear diagnosis of brucellosis. The course of antibiotics needs to be adjusted according to the patient's course and severity. The goal of treatment is to control the disease and prevent complications, disease recurrence and sequelae.
Physicians usually use a combination of antibiotics to extend the treatment time and choose antibacterial drugs that can enter the cell, such as doxycycline and rifampicin.
The treatment plan for patients who have developed spondylitis, neurological brucellosis, and brucella endocarditis will be adjusted appropriately.
Pay attention to rest, supplement nutrition, and give symptomatic supportive treatment if necessary.

Brucellosis prevention

1. Manage the source of infection
Sanitary inspections of livestock in pastures, dairy farms and slaughterhouses are carried out regularly. Sick animals found should be isolated and disposed of in a timely manner and slaughtered if necessary. The abortion of sick and dead animals must be buried. The contaminated environment should be disinfected with 20% bleach or 10% lime milk. Sick animal milk and its products must be boiled and disinfected. Fur should be left for more than three months after disinfection before being transported out of the affected area.
2. Cut off the transmission path
Strengthen sanitary supervision of livestock products, fasting of sick meat and dairy products. Prevent feces from sick animals or patients from contaminating water sources. People who come into close contact with livestock or animal products should be educated and personally protected.
3. Protect vulnerable people and healthy livestock
In addition to protection, an important measure is immunization with vaccines.

Brucellosis vaccine

Brucellosis vaccines include live brucellosis vaccine (I), live brucellosis vaccine (II)
Brucellosis live vaccine (I): This strain is made of attenuated Brucella sheep M5 or M5-90 strains, inoculated in a suitable culture medium, then added a suitable stabilizer to the culture and vacuum Freeze-dried. It is a tan sponge-like loose mass that easily falls off the bottle wall. Dissolve quickly after adding the diluent. This product is used to prevent brucellosis in cattle and sheep, and immunity can last for 3 years. Usage: subcutaneous injection, nasal drops, aerosol immunization and oral immunization. Subcutaneous injections should contain 25 billion viable bacteria, 25 billion viable indoor aerosols, and 40 billion viable outdoor aerosols. Goat and sheep were injected subcutaneously with 1 billion live bacteria, nose drops with 1 billion live bacteria, indoor aerosol with 1 billion live bacteria, outdoor aerosol with 5 billion live bacteria, and oral administration with 25 billion live bacteria.

Which animals can be detected by the BALLYA Brucellosis test?

The BALLYA Brucellosis test can be applied to all kinds of ruminant and pets. The characteristics of this product are short detection time, simple operation and low price.
The test objects of this product include brucellosis antigens and brucellosis antibodies; the test samples include tissue and milk samples.
You can choose according to your needs.

Significance of testing Brucellosis

In order to ensure the safety of consumers, reduce the economic loss of the owner of the breeding plant, prevent the sick ruminant from entering the market, and treat the sick ruminant in time, the Brucellosis Test can play a key role. Ensure the safety of farm owners and consumers, reduce unnecessary losses.

Component of BALLYA Brucellosis Test?

BALLYA Brucellosis Test, 20 cassettes
Swab, 20 pcs
PE Groves, 1 packet
Sample Buffer, 20 vials
Mini Pipette (optional)
Disposable micropipette tips (optional)
Kit Instruction, 1pcs
Brucellosis Test

How to use BALLYA Brucellosis Test?

For ruminant:
1. Sample of serum or whole blood
(1). Pipetting 0.5-1ml of blood, centrifuge for 3-5 minutes at 3000 r/m to separate the serum. It can also be left standing overnight at 4ºc to allow the serum to separate. Or test the whole blood without preprocessing.
(2). Let the test card at room temperature, take out and place it on the flat desk.
(3). Take 5ul (as the black mark on the capillary tube) of the serum or whole blood into the sample well, and immediately add a drop of diluent.
(4). Please wait for 5 minutes.
(5). Add two more drops of diluents.
(6). Stand and read the result within 10-15 minutes. The result after 15 minutes is invalid.
2. Sample of milk
(1). Take the raw milk, ensure the milk is fresh and no heated.
(2). Let the test card at room temperature, take out and place it on the flat desk.
(3). Add 20ul milk into the sample well.
(4). Wait for 5mins.
(5). Add 3 drop of diluents in the sample well.
(6). Stand and read the result within 10-15 minutes. The result after 15 minutes is invalid.

For pets:
1. Pipetting 0.5-1 ml of blood, centrifuge for 3-5 minutes at 3000 r/m to separate the serum. It can also be left standing overnight at 4ºc to allow the serum to separate out by itself. Or test the whole blood without preprocessing.
2. Let the test card at room temperature, take out and place it on the flat desk.
3. Take 5ul (as the black mark on the capillary) of the serum or whole blood into the sample well, and immediately add a drop of diluent by the diluents' dropper.
4. Please wait for 5 minutes.
5. Add two more drops of diluents.
6. You will see a burgundy liquid flowing through the screening hole. Read the result for 10-15 minutes. The result after 15 minutes is invalid.

Limitations of BALLYA Brucellosis Test?

BALLYA Brucellosis Test is a qualitative test kit. It’s only for screening purpose. If have positive cases or suspected case, maybe use other detection method to make a further detection, such as, ELISA, PCR, qPCR, etc.

Conclusion

The spread of brucellosis is closely related to animal husbandry. If a cow is infected with brucella, it cannot be cleaned and isolated in time, and brucellosis will soon occur in the herd, which will increase the chance of infection.
BALLYA Brucellosis Test provided by BALLYA can effectively detect whether cattle have Brucellosis. The kit is not only simple to operate, but also has short detection time and high accuracy, so that the veterinarian can understand the dog's physical condition and take appropriate treatment measures in time.

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