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BALLYA Infectious Bursal Test

Ballya-antibiotic-testBALLYA Infectious Bursal Test

What is BALLYA Infectious Bursal Test?

BALLYA Infectious Bursal Test is manufactured by BALLYA, it’s rapid test for detection Infectious Bursal disease in poultry. Lateral flow assay test base on the gold immunochromatography assay technology.
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What is Infectious bursal disease?

Infectious bursal disease symptoms

The incubation period of the disease is 2 to 3 days, and the onset of susceptible flocks is sudden. The course of disease is usually about one week. The death curve of typical flocks is sharp. One of the early symptoms of the affected chickens is that some sick chickens peck their anus, and then the sick chickens develop diarrhea and discharge white sticky or watery stools. With the development of the disease course, the appetite gradually disappeared, the neck and the whole body trembled, the gait of the sick chicken was unstable, the feathers were fluffy, the spirit was withered, the body temperature was often raised, and the feathers around the cloaca were contaminated with feces. At this time, the sick chicken was severely dehydrated, the toes were dry, the eye socket was sunken, and finally it died. Acute diseased chickens can die after 1 to 2 days of symptoms, and the flocks reach the peak of death in 3 to 5 days, and then gradually decrease. In chicken farms with initial onset, they are mostly infected, with typical symptoms and high mortality. In the future, more cases will be transferred to the sub-clinic type. In recent years, it has been found that the type of disease caused by some type I mutants is mostly sub-clinical, with low mortality, but the immunosuppression caused by it is severe.

Pathogenesis of infectious bursal disease

After ingestion, the virus destroys the lymphoid follicles in the bursa of Fabricius as well as the circulating B-cells in the secondary lymphoid tissues such as GALT (gut-associated lymphoid tissue), CALT (conjunctiva), BALT (Bronchial) caecal tonsils, Harderian gland, etc. Acute disease and death is due to the necrotizing effect of these viruses on the host tissues. Kidney failure is a common cause of mortality. If the bird survives and recovers from this phase of the disease, it remains immunocompromised which means it is more susceptible to other diseases.

What is Infectious bursal disease virus?

Infectious bursal disease virus is a family of double RNA viruses. The virus has strong resistance, can tolerate general acid disinfectants, and alkaline disinfectants can kill quickly.

Infectious bursal disease virus size

Electron microscope observations show that IBDV has two different sizes of particles, large particles are about 60 nm, small particles are about 20 nm.

Infectious bursal disease virus structure

Electron microscopy showed that IBDV has two different sizes of particles, about 60 nm for large particles and about 20 nm for small particles, both of which are icosahedral three-dimensional symmetrical structures. Viral particles are non-encapsulated and consist of only nucleic acids and capsids. Nucleic acid is double-stranded double-segment RNA, and the capsid is composed of a layer of 32 shells arranged in a 5: 3: 2 symmetrical form.

Infectious bursal disease virus spread

The virus can be transmitted not only through gastrointestinal and respiratory infections, but also through virus-contaminated eggshells, but there is no evidence of transmission through the egg. In addition, the conjunctiva can also spread.

The Infectious bursal disease in poultry

Infectious bursal disease in chickens

Chicken infectious bursal disease (IBD), also known as Gamboro disease, is an acute, highly infectious disease caused by infectious bursal virus. Due to the sudden onset of the disease, short course of disease, high mortality, and immune suppression in chickens, it is still one of the major infectious diseases in the chicken industry. In recent years, through the joint efforts of scientific research departments and the majority of veterinary workers, the disease has been effectively controlled to a certain extent.

Infectious bursal disease diagnosis

(1) Dead chickens are severely dehydrated. Large muscle spots or lumps can be seen in the leg muscles and pectoral muscles.
(2) French cysts are enlarged, purulent, and sometimes bleeding.
(3) Swelling of the kidney and uric acid.
(4) Mucosa at the junction of glandular stomach and muscle stomach sometimes bleeds.
(5) Due to the rapid onset, the disease returned to normal quickly after 3 to 4 days of high death. The cyst of France has enlarged, purulent, bleeding, and atrophic. The above symptoms can be diagnosed as the disease.

How to treat Infectious bursal disease?

(1) Highly immune serum injection of chicken infectious bursal disease. For 3-7 week-old chickens, intramuscular injection is 0.4 ml; for large chickens, doses should be increased; for adult chickens, 0.6 ml can be injected once.
(2) Chicken infectious bursal disease high immunity egg yolk injection, intramuscular injection of 1 ml per kilogram of body weight, has a good therapeutic effect.
(3) Compound ethynone, 0.5 kg chicken 1 tablet per day, 1 kg chicken 2 tablets per day, orally for 2 to 3 days.
(4) Testosterone propionate, 3 to 7-week-old chickens, each muscle injection of 5 mg, only once injection.
(5) Quick-acting tube capsule powder, 0.25 grams per kilogram of body weight, mixed in feed or directly taken orally, and it will be effective 8 hours after taking the medicine, and even fed for 3 days. The cure rate is high.
(6) 8 tablets of morphine guanidine hydrochloride (0.1 g per tablet), 1 kg of ingredients, 15 g of Banlangen granules, dissolved in drinking water. For half-day drinking, the above is a daily amount of 20-25 chickens, 3 days as a course of treatment.

Infectious bursal disease vaccine

The vaccines currently used are mainly inactivated vaccines and live vaccines. The inactivated vaccine mainly includes tissue-inactivated vaccine and oil adjuvant-inactivated vaccine. The use of inactivated vaccine has a good effect on chickens that have been inoculated with live vaccines, and protects chicks with maternal antibodies for 4 to 5 weeks. Vaccination routes include injections, nose drops, eye drops, drinking water, and other immunization methods. Specific choices can be made according to the type, nature, age of the vaccine, and breeding management of the vaccine.

Infectious bursal disease prevention

(1) Pay attention to sanitation in the field and strengthen disinfection and epidemic prevention.
(2) Broilers drink water with live toxic vaccine at one week and two weeks of age.
(3) Breeders and laying hens are strengthened once a week, two weeks old, eight weeks old, twelve weeks old, eighteen weeks old, and every three months after laying.

Which animals can be detected by the BALLYA Infectious Bursal test?

The BALLYA Infectious Bursal test can be applied to all kinds of poultry. The characteristics of this product are short detection time, simple operation and low price.
BALLYA Infectious Bursal test has two detection targets for Infectious Bursal detection: Infectious Bursal antigen and Infectious Bursal antibody. You can choose according to your needs.

Significance of testing Infectious Bursal

In order to ensure the safety of consumers, reduce the economic loss of the owner of the breeding plant, prevent the sick poultry from entering the market, and treat the sick poultry in time, the Infectious Bursal Test can play a key role. Ensure the safety of farm owners and consumers, reduce unnecessary losses.

Component of BALLYA Infectious Bursal Test?

BALLYA Infectious Bursal Test, 25 cassettes
Sample Buffer, 1 vial
Mini Pipette (optional)
Disposable micropipette tips (optional)
Kit Instruction, 1pcs
Infectious Bursal Test

How to use BALLYA Infectious Bursal Test?

1. IBD Sample (Recommended)
From the IBD
1) Dissect the chicken, find the IBD along the rectum.
2) Cut open the IBD, spin the cotton swab to collect the sample in the rectum.
3) Insert the swab to the buffer solution test tube immediately. Spin the swab until the reagent mix with the sample (not less than 10 times). Squeeze the swab on the inner tube wall to squeeze out the liquid.
4) Put the test card on the flat table, absorb the supernatant and add 5 drops into the sample well carefully.
5) Stand for 15-20mins at room temperature and read the result. The result after 20 minutes is invalid.
From the IBD Tissue Homogenate Sample
1) Dissect the chicken, find the IBD along the rectum.
2) Cut down a piece of IBD tissue (5mm*5mm*5mm).
3) Mix the sample with buffer solution as the homogenate.
4) Put the test card on the flat table, absorb the supernatant and add 5 drops into the sample well carefully.
5) Stand for 15-20mins at room temperature and read the result. The result after 20 minutes is invalid.

2. Cloacae Sample
1) Spin inner the cloacal to collect the sample with a cotton swab.
2) Insert the swab to the buffer solution test tube immediately. Spin the swab until the reagent mix with the sample (not less than 10 times). Squeeze the swab on the inner tube wall to squeeze out the liquid.
3) Stand for 1-2mins if need to dissolve the big particle.
4) Put the test card on the flat table, absorb the supernatant and add 5 drops into the sample well carefully.
5) Stand for 15-20mins at room temperature and read the result. The result after 20 minutes is invalid.

Limitations of BALLYA Infectious Bursal Test?

BALLYA Infectious Bursal Test is a qualitative test kit. It’s only for screening purpose. If have positive cases or suspected case, maybe use other detection method to make a further detection, such as, ELISA, PCR, qPCR, etc.

Conclusion

Due to the sudden onset of the disease, short course of disease, high mortality, and immune suppression in chickens, it is still one of the major infectious diseases in the chicken industry. Once it happens, it will cause huge economic losses.
BALLYA Infectious Bursal Test provided by BALLYA can effectively detect whether the chicken has infectious bursal disease. This kit is not only simple to operate, but also has a short test time and high accuracy. Allow the veterinarian to respond accordingly.

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