Gold nanoparticles for sale
Product Name: BALLYA Gold Nanoparticles (Ultra High Concentration Gold Nanoparticles)
Size: 20nm~160nm
Ingredient: Gold nanoparticles + purified water
Concentration: 100 OD (±5OD)
Customization: 100 OD or 200 OD
Consistency of Gold Nanoparticles Size
Use Particle Size Analyzer to analyze the consistency of gold nanoparticles size. If PDI value less than 0.2, then mean it has good a consistency of gold nanoparticles size. Here are three size of BALLYA Gold Nanoparticles size in the below figures with 40nm(PDI: 0.067), 50nm(PDI: 0.121) and 60nm(PDI: 0.094).
Malvern Particle Size Analyzer Analysis (PDI should be less than 0.2)
| Item | Particle size | Size average |
| 60-0.1 (10x dilute) | 67.31 | 66.81 | 66.38 | 66.83 |
| 60-0.05 (20x dilute) | 65.73 | 65.19 | 65.24 | 65.39 |
| 60-0.02 (50x dilute) | 68.19 | 68.03 | 67.90 | 68.04 |
| 50-0.1 (10x dilute) | 56.59 | 56.27 | 56.17 | 56.34 |
| 50-0.05 (20x dilute) | 56.81 | 56.94 | 56.58 | 56.78 |
| 50-0.02 (50x dilute) | 53.86 | 53.32 | 53.54 | 53.57 |
| Item | PDI | PDI average |
| 60-0.1 (10x dilute) | 0.153 | 0.156 | 0.148 | 0.152 |
| 60-0.05 (20x dilute) | 0.171 | 0.165 | 0.165 | 0.167 |
| 60-0.02 (50x dilute) | 0.150 | 0.164 | 0.147 | 0.154 |
| 50-0.1 (10x dilute) | 0.199 | 0.198 | 0.190 | 0.196 |
| 50-0.05 (20x dilute) | 0.191 | 0.185 | 0.185 | 0.187 |
| 50-0.02 (50x dilute) | 0.182 | 0.187 | 0.203 | 0.191 |
Gold Nanoparticles Shape
| Normal Gold Nanoparticles | BALLYA Gold Nanoparticles |
| Irregular shaped | Circle shaped | Circle shaped |
Triangle\Rhombus\Straight
Oval\Square\Irregular |
The irregular shaped nanoparticles take too much in the colloidal gold liquid. That will cause:
1. Easy to be dead
2. Difficult to coated with antibody/antigen/protein
3. Take too much volume of antibody/antigen/protein while labeling
4. Take too much volume of nanoparticles while labeling
5. Sedimentation
6. Aggregation | Circle shaped more than 95%
CV less than 8%
|
Gold Nanoparticles Comparison
| Items | Normal Gold Nanoparticles | BALLYA Gold Nanoparticles |
|  | |
| Dead gold nanoparticles | Yes | No |
| Aggregation | Yes | No |
| Sedimentation | Yes | No |
| Increasing labeling materials | Yes | No |
| Reducing labeling materials | No | Yes (max save 80%) |
| High concentration | No (1 OD - 20 OD) | Yes (100 OD - 200 OD) |
| Usage volume of gold nanoparticles | Larger | Less |
| Surfactant | Not include | Not include |
| Labeling time | Long | Short |
| Stability | 1-6 months | 12 months |
| Manufacturing capacity | 1-30L | 100L at a time |
| Batch consistency | Low | Extremely high |
| False postive | High | Low |
| Increase sensitivity | No | Yes |
| Storage temperature | 4-8 degree | Room temperature shipment, storage 4-8 degree |
Advantages of BALLYA Gold Nanoparticles
Monodisperse Gold Nanoparticles
New production technique: gold nanoparticles keep in circle shaped >95%; high monodisperse and keep ‘social distance’ between nanoparticles; Avoid aggregation & sedimentation
Consistency: Irregular shaped nanoparticles less than 5%, CV% less than 8%
Stability & Shelf Life: 12 months
Batch production technique: Max 100L manufacturing at a time, reduce coefficient of variation (CV) between batches
Benefits of BALLYA Gold Nanoparticles
1. No dead gold nanoparticles
2. No aggregation
3. No Sedimentation
4. >95% circle shaped gold nanoparticles
5. Low CV%: less than 8%
6. Increasing sensitivity
7. Increasing specificity
8. Increasing performance of finish product and keep in same consistency
9. Color band in high brightness and clear
10. No fracture in color band
11. 100L per batch manufacturing, help you reduce CV% between different batches of gold nanoparticles
12. Save up to 80% labeling materials (antigen, antibody, protein)
What happen after use BALLYA Gold Nanoparticles?
Ultra High Concentration Gold Nanoparticles
1. Maximum reduce up to 80% of labeling materials
Evaluation in manufacturing 10,000
colloidal gold rapid test for African Swine Fever
| Item | Normal Gold Nanoparticles | BALLYA Gold Nanoparticles |
| Labeling materials | 5mg | 2mg |
| Usage volume of gold nanoparticles | 1500ml (1 OD) | 37.5ml (40 OD) |
| Operation time | 1 day | 4h |
| Production period | 4 days | 3 days |
Remark: above datas maybe changed according to production condition and technique, only for reference!
Evaluation in manufacturing colloidal gold rapid test for HIV
| Labeling Materials | Labeling volume (ug/ml) | Save |
| 1 OD gold nanoparticles | 40 OD gold nanoparticles |
| HIV Antigen 1 | 2.6 | 1.2 | 53.85% |
| HIV Antigen 2 | 1.6 | 1.2 | 25% |
| HBsAg Antibody | 5.8 | 1.1 | 81.03% |
Remark: above datas maybe changed according to production condition and technique, only for reference!
Evaluation in manufacturing colloidal gold covid 19 rapid test
| Labeling Materials | Labeling volume (ug/ml) | Save |
| 1 OD gold nanoparticles | 40 OD gold nanoparticles |
SARS-CoV-2 antigen
ASARS-27 | 2.8 | 1.3 | 53.57% |
SARS-CoV-2 antigen
ASARS-28
| 3.2 | 1.5 | 53.13% |
Remark: above datas maybe changed according to production condition and technique, only for reference!
2. Save manpower and labeling materials
3. Improve productivity
4. Use Desktop centrifuge enough to do mass production
5. Reduce batch CV%, keep finish products in consistency
6. Short the development cycle at least 50%
Types of BALLYA gold nanoparticles
| Cat. No. | Size (nm) | Concentration | Package |
| BGN00040 | 40 | 100 OD | 10ml - 1000ml |
| BGN00050 | 50 |
| BGN00060 | 60 |
| BGN00070 | 70 |
Order BALLYA Gold Nanoparticles
Frequency Questions and Answers
How to use BALLYA Gold Nanoparticles?
1. Prepare the most suitable PH’s buffer solution
2. Add buffer solution directly to 100 OD gold nanoparticles dilute till 40 OD
3. Add protein into 40 OD gold nanoparticles liquid to label
What’s recommended labeling concentration?
40 OD. At this concentration, it help increase sensitivity, save labeling materials, enhance band color intensity.
What’s the best labeling volume?
It will depend on your production technique and product type, such as, hcg, hiv, covid 19...etc. All are different. Need to do some research.
How to make buffer solution with the most suitable PH?
Please check the formulas of buffer solution.
How to find out the most suitable PH in your technique system?
1. 100 OD concentration of colloidal gold, dilute to 40 OD with different pH buffer solution
2. Add the same quality antibody (recommended 50 ug/ml), reaction 2 hours
3. After the reaction is completed, take a small amount of labeled colloidal gold, and then dilute to 1 OD with the corresponding pH.
4. In the 1 OD labeled-gold, add 10% NaCl to make 1% concentration, observe the color after mixing, the color is the best, the lowest or without aggregation & sedimentation, then, that is the most suitable pH
What’s buffer formula suitable for BALLYA Gold Nanoparticles?
There are 3 types common buffer solution as below:
Acidity based buffer solution (0.01 mol/L)
| pH | 0.01M Citric Acid (ml) | 0.01M Trisodium citrate dihydrate(ml) |
| 3.0 | 18.6 | 1.4 |
| 3.6 | 14.9 | 5.1 |
| 4.0 | 13.1 | 6.9 |
| 4.6 | 10.3 | 9.7 |
| 5.0 | 8.2 | 11.8 |
| 5.6 | 5.5 | 14.5 |
| 6.0 | 3.8 | 16.2 |
| 6.6 | 1.4 | 18.6 |
Citric Acid: C6H8O7·H2O: 0.01 mol/L; Liquid: 2.101g/L
Trisodium citrate dihydrate: Na3 C6H5O7·2H2O：0.01mol/L; Liquid: 2.941 g/L
Alkaline based buffer solution (0.02 mol/L)
| pH | 0.02M borax (ml) | 0.02M boric acid (ml) |
| 7.4 | 1.0 | 9.0 |
| 7.6 | 1.5 | 8.5 |
| 7.8 | 2.0 | 8.0 |
| 8.0 | 3.0 | 7.0 |
| 8.2 | 3.5 | 6.5 |
| 8.4 | 4.5 | 5.5 |
| 8.7 | 6.0 | 4.0 |
| 9.0 | 9.05 | 1.05 |
Borax: Na2B4O7·10H2O,0.005M; Liquid: 1.907 g/L
Boric acid: H2BO3, 0.02M; Liquid: 1.237 g/L
Borax is easy to lose crystalline water, and must be stored in a bottle with a plug.
Buffer solution (Citric Acid + borax) (0.01+0.05)
| pH | 0.01M Citric Acid (ml) | 0.05M borax (ml) |
| 4.4 | 6 | 4 |
| 4.9 | 6.5 | 3.5 |
| 5.5 | 7 | 3 |
| 6.0 | 7.2 | 2.8 |
| 7.8 | 7.5 | 2.5 |
| 8.4 | 8 | 2 |
| 8.8 | 8.7 | 1.3 |
| 9.1 | 9.5 | 0.5 |
Borax: Na2B4O7·10H2O,0.005M; Liquid: 1.907 g/L
Citric Acid: C6H8O7·H2O: 0.01 mol/L; Liquid: 2.101g/L
Borax is easy to lose crystalline water, and must be stored in a bottle with a plug.
The recommended labeling technique for BALLYA Gold Nanoparticles
Physical adsorption method:
Procedures as below:
1. Select the most suitable pH for the labeling protein
2. Select the most suitable buffer solution
| Alkaline based buffer solution | 0.02M borax + boric acid |
| Acidity based buffer solution | 0.01M Citric Acid + Trisodium citrate dihydrate |
| Buffer solution (Citric Acid + borax) | 0.01M Citric Acid + 0.05M borax |
3. Directly dilute from 100 OD to 40 OD
4. Add protein into 40 OD gold nanoparticles liquid, shock or stir till well-distributed and reaction 2hours
5. Add blocking solution, such as, BSA, OVA, etc. Reaction 20 min
6. Centrifuge, remove supernatant, dilute to proper pH
Can we use other labeling technique for BALLYA Gold Nanoparticles?
Yes, of course. Also can use chemical method to label.
Ingredients of BALLYA Gold Nanoparticles
Only two ingredients:
1. Gold nanoparticles
2. Purified Water
Why can not detect PH in BALLYA Gold Nanoparticles?
It’s ultra high concentration, 100 OD. It always be neutral, no need to waste a probes to detect it. It can easy to hurt a pH probes.
What’s the PH of BALLYA Gold Nanoparticles?
It’s always neutral. pH=7
Why will occur dead gold nanoparticles while blocking?
It’s a common question. The different brands’ BSA has their own technique that maybe influence the performance of gold nanoparticles, such as, blocking to dead, dead in drying procedure, reducing sensitivity, increasing false positive, etc. In that case, please change the different brand different batches blocking solution or use the non-protein polymer blocking agents, such as, Polyethylene glycol, etc.
Why use ultra high concentration gold nanoparticles will reduce labeling effectiveness?
I guess you are facing the HOOK EFFECT (overloading antibodies, antigen or proteins).
For example, as usual, 1 OD should be labeled with 5ug/ml, so, while use 40 OD maybe you think it should be used 5ug/ml x 40 = 200ug/ml. But it’s not the truth. Over thousands times experimental, we found that only use 50ug/ml can feed it (40 OD) to be saturated! So, try 50ug/ml, maybe it’s surprise to you.
Precautions
Storage & shipment: room temperature shipping, storage at 2- 8°C, do not refrigerate
Transfer: while transfer or distribute, use clean bottle to avoid dead gold nanoparticles
Shelf life: 12 months
