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Serological pipette

What is a serological pipette?

Posed on  December 2, 2020, edited by Jason, category  
serological pipettes
serological pipettes

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What is a serological pipette?

1. Definition

Serological Pipette, also known as disposable pipettes, is mainly used to accurately measure a certain volume of liquid and needs to be used with other proper pipettes.

The pipette is a measuring device used to accurately pipette a certain volume of solution. The pipette is a measuring pipette that is only used to measure the volume of solution it releases. It is a slender glass tube with an enlarged part in the middle. The lower end is sharp-mouthed, and a marking line is engraved on the upper end of the neck, which is a mark of the exact volume to be removed.

Serological pipette is temperature-calibrated laboratory tools, ideal for laboratories that need to transfer large amounts of liquid (1 mL to 50 mL). A serological pipette is designed in a variety of different forms, including plastic, sterile, reusable, and glass, providing multiple options for the laboratory.

2. Material and specifications

The widely used manufacturing materials are polystyrene, glass, etc. There are mainly 7 capacity specifications, namely 1.0ml, 2.0ml, 5.0ml, 10.0ml, 25.0ml, 50.0mL, 100ml. There are different color rings to mark different capacity specifications, except that the tube body has different precision scale marks, which is more convenient for identification and use at work. Some pipes’ ends are equipped with filter plugs to better prevent cross-contamination when drawing samples.

3. Application

It is widely used in tissue culture, bacteriology, clinical, scientific research experiments and other fields that require aseptic operation to transfer liquids. It is a disposable consumable in the laboratory.

The advantages of serological pipette

1. One - piece structure

These pipettes are made of one piece of polystyrene plastic, which eliminates the solder joints common in other pipettes. Some pipette manufacturers weld the tip and mouth parts of the pipette to the pipette body. These "welded spots" trap liquid and may cause errors. The serological pipette is made of smooth one-piece FDA-grade polystyrene plastic, adopting a seamless design to eliminate the possibility of residual liquid. This unique design provides a faster filling and release rate, while also ensuring the complete flow of the sample and providing higher accuracy.

2. Aerosol barrier filter (plug)

Serological pipette uses an aerosol stopper to prevent liquid and liquid vapor from contaminating the pipette and sample. Unlike tampons, an aerosol barrier filter made of bonded polyolefin fibers provides better protection and reduces the possibility of excessive pipetting and potential contamination of pipetting devices.

3. Ergonomics

Serological pipettes are shorter than most other pipette types. The short and compact container design of these pipettes makes them easier to handle and move throughout the laboratory. You will find that working long hours in cramped spaces and laboratory fume hoods will be more comfortable with a serological pipette.

4. Convenience

The specially designed tip can hold more viscous liquid. The inner diameter of serological pipette ranges from 1.56 mm for 1 mL to 4.50 mm for 25mL.

5. Accuracy

The serological pipette has been printed with a black scale to prevent ambiguity when measuring the amount of fluid aspirated or dispensed. This function can ensure accurate readings, and the error is less than 2% at full scale. The reverse scale applies to all five volumes, and the negative scale is included in all pipettes (except for the 25 ml volume). As far as accuracy is concerned, the accuracy of serological pipettes is the same as the smallest increment (usually 0.1 ml, minimum 2 ml). If your research requires higher precision, we recommend that you use a serological laboratory pipette in combination with a micropipette to achieve best results.

6. Packaging

Serological pipettes are usually disposable and pre-sterilized. They are usually pre-packaged into bulk packages between 25 and 50. To increase productivity, all serological pipettes are designed to fit electronic pipette controllers and pipettes. This universal compactness ensures that you can always work accurately and quickly.

How to use a pipette?

According to the volume and requirements of the transferred solution, select a pipette with appropriate specifications. In the titration analysis, the pipette is generally used to accurately pipette the solution and control the amount of test solution.

Steps for usage:

1. Choose a pipette of appropriate specifications according to the volume and requirements of the transferred solution. In the titration analysis, the pipette is generally used to accurately pipette the solution and control the amount of test solution.

2. Check the instrument: check whether the nozzle and tip of the pipette are damaged. If they are damaged, they cannot be used;

3. Clean the instrument: rinse with water first, then soak in chromic acid washing solution, the operation method is as follows:

  • Hold the pipette or the upper end of the pipette in your right hand. The index finger is close to the upper mouth of the pipe. The middle and ring fingers are spread out to hold the outside of the pipette. The thumb is held in the middle of the middle finger and ring finger on the inside of the pipette. The little finger is naturally relaxed;
  • Hold the ear-washing pipette bulb in your left hand in a fist style, hold the ear-suction ball tightly in your palm with the tip downwards, expel the air from the ball, and insert or close the tip of the ear-suction ball to the pipette (Pipette). Take care not to leak air.
  • Slowly loosen the fingers of the left hand, slowly absorb the washing liquid into the tube until the part above the scale line, remove the ear ball, and quickly block the upper mouth of the pipette with the index finger of the right hand. After waiting for a while, return the washing liquid to the original bottle.
  • Rinse the inner and outer walls of the pipette with tap water until there are no water droplets, then wash it with distilled water 3 times, and dry it for use.

4. Absorb the solution: Shake the solution to be aspirated, pour a small portion of the solution to be absorbed into a clean and dry small beaker, absorb the water inside and outside the cleaned pipette with filter paper, and insert it into the small beaker and absorb the solution. When it reaches 1/3 of the volume of the pipette, immediately press the mouth of the pipette with your right index finger, take it out, hold and turn the pipette horizontally to make the solution flow all over the inner wall of the pipe, and remove the solution from the lower tip end into the waste liquid cup. In this way, the solution can be absorbed after 3-4 rinses.

5. Insert the pipette rinsed with the liquid to be absorbed into 1~2 cm below the surface of the liquid to be absorbed and use the ear ball to absorb the solution according to the above operation method. When the liquid level in the tube rises to about 1~2 cm above the marking line, quickly block the mouth of the tube with your right index finger, lift the pipette out of the liquid surface, and bring the tube into contact with the inner wall of the container t for a while and lift it up with filter paper. Wipe off the small amount of solution adhering to the bottom of the pipette.

6. Adjust the liquid level: Take another clean small beaker with your left hand, put the pipette tip close to the inner wall of the small beaker, keep the small beaker tilted, keep the pipette vertical, and keep the graduation line horizontal to the sight.

Slightly loosen your index finger to allow the solution in the tube to slowly flow out from the lower mouth. When the liquid is close to the mark, press the right index finger firmly, pause for a while, and then obey the upper method to place the bottom line of the meniscus of the solution to the edge of the mark. As soon as it is tangent to the upper mark, immediately press the mouth of the tube with your index finger. Place the tip close to the inner wall of the beaker and move a little to the mouth of the beaker to remove the droplets at the tip. Carefully move the pipette to the container holding the solution.

7. Discharge the solution: Put the pipette upright, tilt the receiver, the lower end of the tube is close to the inner wall of the receiver, let go of your index finger, let the solution flow down the inner wall of the receiver, after the solution in the tube is finished, keep it in the state of discharging for 15s, slide the pipette or pipette a small distance against the wall at the point of the receiver to remove the pipette.

8. Clean the instrument: clean the pipette and place it on the pipette rack.

pipette-bulb
pipette-bulb

Conclusion

Serological pipette is made of transparent polymer material polystyrene (GPPS) and are widely used in clinical and scientific research experiments such as tissue culture and bacteriology. It can greatly reduce the adhesion of liquid to the tube wall and improve the accuracy of sampling. The rigid two-way scale design meets the requirements of sample loading and sample reduction, easy-to-read scales, and extra-volume negative scales, which are convenient for liquid absorption and reading. Both ends are equipped with cotton filter plugs to prevent back suction and overflow.

References

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