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Canine Distemper Virus Test

cat-fsaa-test-kit

For pet disease diagnosis

Specs:25T / box
Testing Time:7-10 mins
Worldwide shipping, free sample
Introduction
The Canine Distemper Virus Test is based on Colloidal gold immunochromatography to detect the immunoglobulin of canine. 

Components
Test Kit
10 pcs
Swab
10 pcs
Dropper
10 pcs
Buffer Solution
10 bottles
Product Instruction
1
Storage & Shelf-Life
At 2~30℃, out of sun light & moisture, 24 months

Test Procedure
1. (1). Used the swab to dip the normal saline and collect the sample from urine or conjunctival mucosa. Then insert the swab to the test tube immediately. Spin the swab until the reagent mix with the sample (not less than 10 times). Squeeze the swab on the inner tube wall to squeeze out the liquid. Add the serum or blood plasma into the diluents and completely mix.
(2). Collect the serum or blood plasma, add 2 drops into the buffer solution and completely mix.
2. Put the test card on the flat table, absorb the supernatant and add 5 drops in the sample well carefully.
3. The liquid will climb, if not, please squeeze the place between sample well and screening hole.
4. Read the result within 10-15 minutes. The result after 15 minutes is invalid.

Interpretation of Results
Positive: line C&T appear
Negative: only line C appear
Invalid: line T&C does not appear or only the line T appear.

Results Illustration
Positive:
Negative:
Invalid:
Precautions
Refer to the instruction

Operation Guideline

If you want to know more, please contact us

Trivia questions : What is canine distemper?

Canine distemper

Canine distemper cause

Canine distemper is a highly contact infectious disease caused by canine distemper virus, which is highly contagious and has a mortality rate of more than 80%.

Canine distemper symptoms

Canine distemper virus infection mainly causes high fever, purulent snot, pus, eye drops, and even eye drops that can cause blindness, corneal damage, cough and even pneumonia. Later stages are mainly neurological symptoms, muscle twitches, and so on. These are more typical symptoms. Some dogs may not have particularly obvious symptoms, one or two symptoms, and some typical all symptoms may occur.

Canine distemper diagnosis

Blood changes in experimental CDV-infected dogs include: lymphocyte proliferation, thrombocytopenia, and aplastic anemia, but it is not common in clinical cases. Occlusions are occasionally found in acutely infected blood smears (sometimes lymphocytes and neutrophils). Buffy coat smears and examination of bone marrow extracts can also show intracellular inclusions. Detection of canine plague increased. In addition, canine distemper inclusions can be detected from conjunctival epithelial cells. Cerebrospinal fluid examination can show that increased intracranial pressure causes increased cerebrospinal fluid flow, increased protein concentration (25mg / dL. [250mg / L]), and decreased lymphocytes. These changes are characteristic of canine plague infections, but are not specific. Cerebrospinal fluid IgG can be used to diagnose CDV infection. Immune-inducing antibodies are rarely detected in cerebrospinal fluid. Although there are many other diagnostic methods for CDV, such as immunological methods, using enzyme-linked immunosorbent assay (ELISA) to measure IgG and IgM antibodies in serum, and isolating viruses, they require special equipment and sample preparation. Post-mortem examination is the most effective way to diagnose CDV where it may be infected. Brain and cerebellum white matter examinations should also be taken seriously.

Canine distemper transmission

The disease "occurs all seasons" throughout the year, but occurs frequently in "spring and winter". The disease has a certain periodicity, with a pandemic "every three years".
Route of transmission
[Sick Dog] A sick dog that infects canine distemper.
[Secret / Excretion] For example: eye secretion, nasal fluid, saliva, pericardial fluid, pleural fluid, ascites, urine, feces, etc. Dogs that have been infected with canine plague virus have reported that the virus is still excreted in the urine after 60-90 days, so urine is a very dangerous source of infection.
[Blood] Cerebrospinal fluid, lymph nodes, liver, spleen, spinal cord and other organs contain numerous viruses, and can be detoxified to the outside with respiratory secretions and urine.
[Air] Air contaminated by sick dogs, such as droplets, through respiratory infections.
[Contact] Infected after contacting a sick dog.
[Diet] Infection through the respiratory or digestive tract due to diet.
[Hint] Once dogs of the same room are found, no matter how strict protective measures are taken, it is difficult to avoid mutual infection.

Canine distemper incubation period

The incubation period of canine distemper varies greatly with the source of infection. The incubation period is 3-6 days. The incubation period from xenobiotics can sometimes be as long as 30 to 90 days.

Canine distemper virus

Canine distemper virus (CDV) belongs to the family Paramyxoviridae and measles, and is a single-stranded RNA virus. At room temperature, the virus is relatively unstable, especially sensitive to ultraviolet rays, dryness, and high temperatures above 50-60 °C (122-140 °C).
Under freezing conditions, the virus can survive for weeks. Most routine disinfections can kill CDV.

Canine distemper virus mechanism

The initial infection with CDV started when the virus attacked upper respiratory epithelial cells. The virus quickly spread to localized lymphoid tissues, tonsils, and bronchial lymph nodes. CDV then spreads to epithelial tissues and the central nervous system during the first week of infection. The humoral immune response after infection is related to the elimination of the virus. Dogs recovering from an acute CDV infection are likely to gain years of immunity. After a dog with no effective immunity is infected, the virus will spread to the skin, endocrine and exocrine glands, digestive tract, respiratory tract, and urogenital tract.

The danger of canine distemper virus

CDV is one of the oldest and most clinically significant viruses that infect dogs. CDV infection manifests a variety of symptoms, which are related to viral virulence, environmental conditions, the age of the host, and immune status. They can be divided into five characteristic types: superacute, acute, gastrointestinal symptoms, neurological symptoms and skin symptoms. Once characteristic symptoms appear, the prognosis is extremely poor.

Canine distemper treatment

Early and medium-term treatment of canine distemper:
Dogs whose digestive tract has been affected by canine distemper virus often do not eat well. In this case, infusion support therapy is required, but a large amount of infusion will cause pulmonary edema, so it is not appropriate to infuse more. Generally, infusion can be performed at 20-50ml per kilogram of body weight. Conditionally, you can do blood gas analysis to calculate the amount of blood ions that need to be added:

There is no good cure for canine distemper in its later stages. Current treatments focus on controlling secondary infections of the respiratory tract and conjunctivitis.

Canine distemper prevention

First, canine vaccination can be prevented by vaccination. Vaccination can be started at 6 to 8 weeks of age, and then vaccinated twice consecutively every three weeks. After that, boosters can be given annually or every three years, depending on the risk of infection.
Second, when buying dogs, it is not recommended to buy from a crowded pet market. Dogs bred in this environment are very likely to contract canine plague.

Canine distemper vaccine

CDV highly attenuated vaccines have been used on the market for many years. The vaccine has a good safety record. Most manufacturers recommend three to eight canine immunizations per week. After immunity is strengthened, immunity can last for 3 to 5 years. Dogs recovered from natural infections can be immune for years. Sometimes, dogs vaccinated with the Rockborn CDV strain have reportedly developed encephalitis after vaccination. Post-vaccination encephalitis usually occurs in newborn puppies who have immunized the bitch during pregnancy or before giving birth.

Summary :

Dogs are lively animals. Even if they meet with their owners separately for a short time, they usually express their thoughts and attachments to their owners with strong physical language. Therefore, it is difficult for dog owners to find the early symptoms of canine distemper and miss the best time for canine distemper treatment.
The Canine Distemper Virus Test kit provided by BALLYA can effectively detect whether a dog has canine distemper. This kit is easy to operate, and the detection time is short and the accuracy is high. It allows the veterinarian to understand the pet's physical condition in time and make the corresponding treatment.
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