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Porcine Parvovirus Ab Rapid Test

cat-fsaa-test-kit

For pig disease diagnosis

Specs:25T / box
Testing Time:7-10 mins
Worldwide shipping, free sample
Introduction
The Porcine Parvovirus Ab Rapid Test is based on the Colloidal gold immunochromatography to detect the whole blood and serum of porcine. This kit can be applied for on-site rapid testing by various departments.

Components
Test Kit
40 pcs
Dropper
40 bottles
Titer Card
1pc
PE Groves
1 packet
Product instruction
1
Storage & shelf-life
At 2~30℃, out of sun light & moisture, 24 months

Test procedure
1. Pipetting the whole blood, centrifuge for 5-15 minutes at 2000-3000 r/m to separate the serum. It can also be left standing overnight at 4℃ to separate out the serum. Or test the whole blood without anticoagulant immediately.
2. Take out and place the card on the flat desk.
3. Absorb the sample and add 3 drops into the sample well carefully.
6. Read the result for 10-20 minutes. The result after 20 minutes is invalid.

Interpretation of results
Positive: line C&T appear
Weak Positive: line T lighter than C
Negative: only line C appear
Invalid: line T&C does not appear or only the line T appear.

Results illustration
Positive:
Weak Positive:
Negative:
Invalid:
Precautions
Refer to the instruction

If you want to know more, please contact us

Trivia questions : What is PPV?

Porcine parvovirus

Porcine parvovirus symptoms

Outbreaks in swine herds are often associated with clinical manifestations such as mummification, reduced litter size, dystocia and repeated breeding. Infection in the early 30-50 days of pregnancy, the embryo dies or is absorbed, causing the sow to become infertile and irregularly estrus.
Infection in the second trimester of pregnancy is 50-60 days. After the fetus dies, a mummy is formed. Fetuses that are more than 60-70 days later in the pregnancy have autoimmune ability and can resist virus infection. Most fetuses can survive but can be infected for a long time.

Porcine parvovirus transmission

Domestic and wild boars of all ages and sexes are susceptible. The source of infection mainly comes from sows infected with parvo and boars with poison. The reserve sows are more susceptible than the sows born. The virus can spread vertically through the placenta. The live pigs produced by the infected pigs may be infected with detoxification time. Long or even lifetime. Infected breeding boars are also the most dangerous source of the disease. The virus can be isolated from boar semen, spermatic cord, epididymis, and gonads. Breeding boars can be transmitted to susceptible sows and spread the disease.

Porcine parvovirus diagnosis

Inspection of viral antigens: (1) Direct staining with PPV fluorescent antibody: Observe under a fluorescent microscope, and if the nucleus is not stained in the inoculated cell sheet, the diagnosis can be confirmed. (2) Direct staining of PPV enzyme-labeled antibody: Observe the staining under a common biological microscope. If the nucleus of the normal control cell sheet not inoculated with PPV has no brown staining, and the nuclear staining of the inoculated PPV cell sheet, that is, can be diagnosed. (3) PPV hemagglutination test: If it is found that the diluted sample has agglutination of red blood cells, but the normal PBS red blood cell control does not have autocoagulation, it can be considered that the sample is suspicious and a specific PPV standard positive serum is used as a hemagglutination inhibition test If you can inhibit the blood coagulation of the sample, you can be diagnosed as PPV.

Porcine parvovirus properties

Porcine parvovirus is a member of the parvovirus family Parvovirus.

Porcine parvovirus size

Virus particles are non-capsule-like, with a diameter of about 20 nm, and the genome is single-stranded DNA, about 5.2 Kb.

Porcine parvovirus structure

The virus has a strong resistance to heat. There is no significant change in the infectivity and hemagglutination of the virus for 48 hours at 56 °C or 2 hours at 70 °C, but both infectivity and hemagglutination activity can be lost for 5 minutes at 80 °C. The virus is extremely stable at 40 °C, has a strong resistance to acid and alkali, is stable between pH 3.0 to 9.0, and can resist lipid solvents such as ether and chloroform, but 0.5% bleaching powder, 1% to 1.5% sodium hydroxide 5 Minutes can kill the virus, 2% glutaraldehyde takes 20 minutes, and formaldehyde vapor and ultraviolet rays take quite a long time to kill the virus.

Pathogenesis of porcine parvovirus

The pathogenesis of PPV-infected pigs is not completely clear. Some research results show that the impact of PPV on pigs can be divided into two aspects: one is the effect on sows fertilized egg cells, and the other is the effect on fetal development.

Porcine parvovirus treatment

There is no cure for this disease. When breeding pigs are introduced, quarantine should be strengthened and pigs should not be imported from the affected areas. Natural infection is required 2 months before mating of primiparous sows in the epidemic area. Serum-negative sows can be mixed with sero-positive sows or inoculated with inactivated vaccines and attenuated vaccines to ensure that new sows are pregnant Before getting active immunity to protect the fetus from harm.

Porcine parvovirus prevention

Take comprehensive control measures: Parvovirus (PPV) is very resistant to the external environment. To maintain an infection-free pig farm, strict sanitary measures must be taken, and self-reproduction as much as possible should be maintained. If breeding pigs are required, Ii must be introduced from a farm free of parvovirus (PPV) infection. When the HI titre is below 1: 256 or negative, the introduction is permitted. Strictly isolate for more than 2 weeks after the introduction. When the HI-negative test is performed again, it can be mixed breeding. The affected pig farms should be specially prevented from becoming infected during the first feeding period. The breeding period can be delayed until 9 months of age, at which time the maternal antibodies have disappeared (maternal antibodies can last for an average of 21 weeks). Breed after artificial immunity to make it immune.

Porcine parvovirus vaccine

Vaccine prevention It is recognized that the use of vaccines is an effective method to prevent porcine parvovirus disease and to improve the resistance and reproductive rate of sows. Parvovirus (PPV) vaccines have been developed in more than 10 countries. Vaccines include live vaccines and inactivated vaccines. The antibody titer produced by the live vaccine is high, and the maintenance time is longer, while the immune period of the inactivated vaccine is relatively short, generally only six months. Vaccination can be performed a few weeks before breeding to keep pregnant sows strong during the susceptibility period. In order to prevent the interference of maternal antibodies, two injections can be used or the HI titer can be determined to determine the immune time. When the antibody titer is greater than 1:20, it is not appropriate to inject. Infection.

Summary

Porcine parvovirus disease has occurred on a large scale in many pig farms in China, causing great economic losses. Although the incidence has now been effectively controlled, it cannot be ignored.
The Porcine Parvovirus Ab Rapid Test kit provided by BALLYA can effectively detect whether pigs have porcine parvovirus disease. This kit is not only simple to operate, but also has a short test time and high accuracy. Allow the veterinarian to respond accordingly.
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