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Oxolinic acid Test For Egg(R&D)

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beta-lactams-Test-Kit

For all kinds of eggs

Specs:96T / box
Testing Time:7-10 mins
Worldwide shipping, free sample

Instrution For Oxolinic acid Test For Egg

Introduction
Single Test Kit, rapid to detect an antibiotic residues in milk and dairy product by using colloidal gold immunochromatography technology. As soon as 7-10 minutes to results.

Application
For raw milk, whole milk powder, Pasteurized milk.

Spicifications
1. Specificity
Not cross-react with macrolides, aminoglycosides, sulfonamides drugs, etc.

2. Limit of Detection
Refer to instruction.

Storage & Shelf-Life
At 2~8℃ out of sunlight, 12 months

Compolents (96T / box)
Instruction                          1
Tube                                     12

Equipments (Option)
Timer
Incubator
Mini Pipette(200μL)
Tips for mini pipette
Reader

Operations
1. Take 200 μL milk specimen, add into micro well, mix with the pink agent 10 times.
Remark: avoid specimen residues too much in the well.
2. Incubate 3 minutes
3. Insert strips into the wells
4. Incubate 5 minutes
5. Take out strips and read results by eye or reader.

Interpretation
Visual inspection
1. If C line visible, interpret according to the instruction. T line compare with C line to interpret positive or negative.
2. If C & T are invisible, it’s invalid detection, repeat testing again.

Reader Interpretation
Refer to the instruction

Results Illustration

If you want to know more, please contact us

Trivia questions: Do you know oxolinic acid?

About oxolinic acid

Oxolinic acid is the second generation of quinolones are white with yellowish white columnar crystals or crystalline powder, odorless and tasteless. Melting point 320 ℃, almost insoluble in water and ethanol, soluble in formic acid and sodium hydroxide solution, does not absorb moisture, and is relatively stable to heat, humidity and light.

Oxolinic acid mechanism of action

Targets the deoxyribonucleic acid (DNA) of bacteria. The double-stranded DNA of bacteria is twisted into loops or spirals (called supercoils). The enzyme that makes DNA supercoils is called DNA gyrase, and quinolones hinder this enzyme, further causing irreversible damage to chromosomes and causing bacterial cells No longer split. They show selective toxicity to bacteria. At present, the resistance of some bacteria to many antibiotics can be widely spread due to plasmid transmission.

Oxolinic acid solubility

It is almost insoluble in water and ethanol, soluble in formic acid and sodium hydroxide solution, does not absorb moisture, and is relatively stable to heat, humidity and light.

Oxolinic acid uses

It has a strong broad spectrum, has a strong antibacterial effect against Gram-negative bacteria and some positive bacteria, and has no cross-resistance with antibiotics, but has no antibacterial effect on fungi and Mycobacterium tuberculosis. It has many advantages such as good bacteria effect. Foreign aquaculture farmers believe that it is one of the more ideal drugs for the treatment of aquatic animal diseases, and it has strong antibacterial activity against fish pathogens such as Vibrio anguillarum and Aeromonas hydrophila.

Oxolinic acid indications

Antibacterial drugs that mainly act on Gram-negative bacteria have a weaker effect on Gram-positive bacteria (some varieties have better antibacterial effects on Staphylococcus aureus).

Oxolinic acid for fish

Overseas aquaculture farmers believe that it is one of the more ideal drugs for the treatment of aquatic animal diseases, and it has strong antibacterial activity against fish pathogens such as Vibrio anguillarum and Aeromonas hydrophila. Therefore, eels and other aquatic animals are widely used in Japan and Taiwan Province. PH in marine aquaculture has a slight impact on it, and the dosage of drugs needs to be increased in alkaline environments. The toxicity of oxolinic acid is very slight, acute, and the half-lethal amount of oral administration to carp (that is, the time that 50% of individuals can die under the influence of a certain concentration of the drug) is more than 4000 mg / kg. The use of the drug must be stopped before 25 days, otherwise the residual amount exceeds the standard, and the Japanese market is prohibited from importing.

Oxolinic acid toxicity

Acute oral LD50630mg / kg (male), 570 mg / kg (female) for rats; acute oral LD502200mg / kg for male mice, 1450 mg / kg for female mice; acute percutaneous LD50 for rats> 2000 mg / kg, it has no stimulating effect on the skin of Chemicalbook, and has a slight stimulating effect on the eyes. No sensitization. The no-effect dose of 100-300 mg / kg in the 2-year feeding test in rats. Animal experiments have no teratogenic, cancer-causing, and mutagenic effects. It is used as usual and has no effect on fish. Carp LC5010mg / L (48h).

Oxolinic acid interaction

(1) Alkaline drugs, anticholinergic drugs, and H2 receptor blockers can all reduce the acidity of gastric juice and reduce the absorption of this type of drugs.
(2) Rifampicin (inhibitor of RNA synthesis) and chloramphenicol (inhibitor of protein synthesis) can reduce the effects of this class of drugs, completely eliminate the effects of nalidixic acid and haloperic acid, and make fluazamic acid The effect of ciprofloxacin is partially offset.
(3) Fluoroquinolones can inhibit the metabolism of theophylline. When combined with theophylline, the plasma concentration of theophylline will increase, and the toxic reaction of theophylline may occur, so attention should be paid.

Oxolinic acid side effects

The main side effects of this class of drugs are:
① Gastrointestinal reactions: nausea, vomiting, discomfort, pain, etc.;
②Central reactions: headache, dizziness, poor sleep, etc., and may cause mental symptoms;
③Because this class of drugs can inhibit the effect of γ-aminobutyric acid (GABA), it can induce epilepsy, and those with a history of epilepsy should be used with caution;
④ This type of medicine can affect cartilage development, pregnant women and minor children should be used with caution;
⑤ Crystalline urine can be produced, especially in alkaline urine;
⑥ Large-dose or long-term application of this class of drugs is likely to cause liver damage.

Summary

As a veterinary drug, this product is absorbed orally quickly but not completely. It is widely distributed in animals and can enter tissues and body fluids well. Mainly excreted from urine in the form of original drugs. But it is inevitable that there will be residual drugs left in the body. This is likely to cause oxolinic acid residues in chicken or eggs. It is necessary to detect oxolinic acid in eggs.
The Oxolinic Acid Test For Egg kit provided by BALLYA can effectively and quickly detect whether eggs contain oxolinic acid residues. Not only is the operation simple and the detection time is short, it allows the tester to obtain the test results in the fsirst time.

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