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Nicarbazin Test For Chicken(R&D)

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For various types of chicken

Specs:96T / box
Testing Time:7-10 mins
Worldwide shipping, free sample

Instrution For Nicarbazin Test For Chicken

Single Test Kit, rapid to detect an antibiotic residues in milk and dairy product by using colloidal gold immunochromatography technology. As soon as 7-10 minutes to results.

For raw milk, whole milk powder, Pasteurized milk.

1. Specificity
Not cross-react with macrolides, aminoglycosides, sulfonamides drugs, etc.

2. Limit of Detection
Refer to instruction.

Storage & Shelf-Life
At 2~8℃ out of sunlight, 12 months

Compolents (96T / box)
Instruction                          1
Tube                                     12

Equipments (Option)
Mini Pipette(200μL)
Tips for mini pipette

1. Take 200 μL milk specimen, add into micro well, mix with the pink agent 10 times.
Remark: avoid specimen residues too much in the well.
2. Incubate 3 minutes
3. Insert strips into the wells
4. Incubate 5 minutes
5. Take out strips and read results by eye or reader.

Visual inspection
1. If C line visible, interpret according to the instruction. T line compare with C line to interpret positive or negative.
2. If C & T are invisible, it’s invalid detection, repeat testing again.

Reader Interpretation
Refer to the instruction

Results Illustration

If you want to know more, please contact us

Trivia questions: Do you know nicarbazin?

About nicarbazin

It is yellow or yellow-green powder; odorless and slightly odorous. Used to prevent coccidiosis in chickens and turkeys.

Nicarbazin solubility

This product is slightly soluble in dimethylformamide, but insoluble in water, ethanol, ethyl acetate, chloroform and ether.

Nicarbazin mode of action

Used to prevent coccidiosis in chickens and turkeys. This product is effective for the second generation of schizonts of coccidia. The recommended amount does not affect the immunity of chickens against coccidia, and it is safe. Use with caution in hot seasons and ban during egg production. Mixed feed, 125g per 1000 kg feed. The two components of nicarbazin can be absorbed by the digestive tract of poultry, and are widely distributed in tissues and body fluids.

Nicarbazin synthesis

Add 8.1g (0.027mol) DNC, 2.5g (0.042mol) urea, 4mL acetylacetone and a small amount of concentrated hydrochloric acid to 35mL absolute ethanol, stir and reflux to react for 10-15h, cool to room temperature for crystallization, centrifuge, no Chemicalbook water Washed with ethanol and dried to obtain a yellow composite powder, the yield is 80%. The molar ratio of 4,4'-p-nitrodiphenylurea (DNC) and 2-hydroxy-4,6-dimethylpyrimidine (HDP) is 1: 1 compound. HDP is prepared by the condensation reaction of acetylacetone and urea.

Nicarbazin uses

Nicarbazin is mainly used to prevent chicken caecal coccidiosis (Eimeria tenella) and heap type, giant, poisonous, Eimeria brucei (intestinal coccidiosis).
According to the test, medication within 48 hours after infection with coccidia can effectively inhibit the development of coccidia. If the medication is used more than 72 hours later, the effect is significantly reduced.
The recommended dose of nicarbazine has little or no effect on the body's immunity to coccidia. In addition, coccidia that are resistant to amphetamine are still effective.

Nicarbazin poultry

Nicarbazin in feeds

This product is effective against the second generation of schizonts of coccidia and is used to prevent coccidiosis in chickens and turkeys. Mixed feed, 125g per 1000 kg feed.

Nicarbazin toxicity in broilers

Nicarbazin poisoning in broilers generally rarely occurs. Once the disease occurs, the situation is urgent and the treatment is more difficult. To find a way to cool down and solve the symptoms of high fever.
Chickens are mentally exhausted, fasting, fasting, breathing with their mouths open, their heartbeats are accelerated, their body temperature rises, they stay on the ground, they flutter their wings, scream, and struggle before death.

Nicarbazin Precautions

(1) During the preventive treatment of nicarbazine, if a large number of chickens are exposed to infectious oocysts and coccidiosis breaks out, they should be quickly switched to more effective drugs (such as toltrazuril, sulfonamides, etc.)
(2) Because nicarbazine can reduce egg production rate, fertilization rate, egg quality and lighten the brown eggshell, laying hens are prohibited.
(3) Due to the potential growth inhibitory effect of nicarbazine on chicks, it is advisable not to use young chicks under 5 weeks of age.
(4) During the intense heat, if the ventilation and cooling equipment of the chicken house is incomplete and the room temperature exceeds 40 ℃, the application of nicarbazine can increase the mortality of the chicks.
(5) Withdrawal period. Broiler for 4 days.

Nicarbazin side effects

The main reasons why the content of chicken is detected excessively frequently are:
The main ingredient is difficult to metabolize. Nicarbazine is a mixture of two types of substances, 4-4 ’dinitrodiphenylurea (DNC) and 2-hydroxy-4.6-dimethylpyrimidine (HDP), which can be digested and absorbed by the digestive tract
It is collected and widely dispersed in animal tissues and body fluids. Among these two components, HDP basically has no anticoccidial effect, and can be quickly excreted through urine in animals; DNC is the main active component of anticoccidial effects. It is metabolized slowly in animals and can only be excreted through feces.

Overuse. Many breeders apply pesticides at random or do not strictly implement the drug withdrawal period, resulting in excessive DNC content in animals.

Wide source of intake. Although it is stipulated that there should be a 4-day drug withdrawal period before slaughtering of broilers, to ensure the complete metabolism of nicarbazin in chickens, it is still possible for broilers to enter the broiler circulation during the drug withdrawal period because they can contact their feces.


At present, studies on the physiologic toxicity of nicarbazin show that when 80 times the normal dose is taken for a long time, the sperm aberration rate of male rats increases significantly. Therefore, we should pay attention to control the dosage, in actual use
It is safe to use within the dosage range. Long-term consumption of chicken meat with excessive levels of such drugs may pose a certain risk to health.
The detection of nicarbazin is very necessary. The Nicarbazin Test For Chicken kit provided by BALLYA can effectively and quickly detect whether chicken contain nicarbazin residues. Not only is the operation simple and the detection time is short.

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