Aflatoxin, hei a mycotoxin, Ko te metabolite o harore hua ka whakaputa i te witi ranei kai hou kaore i te maroke ka penapenahia i te wa tika, a, he maha nga waa. Na te kaha o te paitini me te mate pukupuku o te tinana, kua whakatauhia nga ture totika mo te paerewa kai me te paerewa whangai. Aflatoxin M1 no te mycotoxin me te ko te metabolite hydroxylation o aflatoxin B1 i roto i nga kararehe. He tino paitini me te mate pukupuku, kia ārai mai i te whakauru ki te kai. Na te mea he kino ki kiko ate a kia whakakiki waiu mate pāwera me te mate pukupuku ate, i whakararangihia hei carcinogen na WHO Te Whare Rangahau Rangahau Mate Mate i te timatanga o te tau 1993.
Na te mea he iti nga korero me nga hanganga rite o te aflatoxin, me tino whai kiko me te motuhake o te tikanga kitenga, e hono ana i te wehenga me te kitenga. I roto i tenei pepa, he pai te whakamaarama o te wai (HPLC) i whakamahia ki wetewete aflatoxin. Ko te raru o te iti o te ihirangi o te aflatoxin i whakaekea, a i kitea he hua nui.
Tauira tauira
Tuhinga ka whai mai mākete miraka ka tangohia 5g ka maka ki roto i te pounamu ine 100mL. Ka rewa te paura miraka ma te taapiri i te wai mahana 20 mL ki te 50 nekehanga Celsius kia piuroto ai mo te 30S. Ka honoa te konutai pūhaumāota kia rewa ai mā te ohorere mō te 2g. Ko te 50 mL acetonitrile ka taapirihia ki te oscillate mo te 30S. I muri i te whakaranu, ka tapirihia te acetonitrile ki te pounamu kia tutuki ai te pauna. Ma te honikoretanga o te panuku e orite ai te ahua. Taputapu, 40 mL o filtrate, 40 C hauota ka heke ki te maroke tata. Ko te toenga i rewa ma te taapiri i te 20 mL 10% acetonitrile. I horoia te pou hononga ki te 10 mL wai i muri i te paahitanga o te tauira i roto i te pou hononga.
I te mutunga, 2 mL acetonitrile i whakamahia mo te whakaahuru. I kohia te 1.5 ml o te eluent ka maroke ki te hauota. Ka taapirihia te 200 uL hexane me te 200 uL trifluoroacetic acid, te wiri me te whakaranu, te uruparenga i te 40 C mo te 10 meneti, ka puta, ka maroke i raro i te rere o te hauota, ka taapirihia he 10% acetonitrile 0.5 ml, ka wiri mo te 10 hēkona.
huru whakamātau
Momo taputapu: Dionex Ultimate 3000 Series
Papu: LPG-3400SD
Tauira Aunoa: WPS-3000SL
Pouaka Temperature Pouaka: TCC-3000RS
Kaitohu: FLD
Pūmanawa Chromatography: Chromeleon Chromatography Pūnaha Raraunga
Momo Kaituku, mahi mahi, me te tau S / N:
Kaitohu Fluorescence, FLD-3400
Te wa whakautu = 1
Ko te Tae Whakatau Ahurangi FlD = 35.00 [C]
ReriRa TereRa ReadyTempDelta = 1.00 [C]
Tikanga Akonga = Apiti
Tauanga Kohinga Kohinga = 10.00 [Hz]
Whakauru 1.ExWavelength = 365.0 [nm]
Whakauru 1. Em Roangaru = 435.0 [nm]
Whakauru 1. Maharahara = 6
Whakauru 1.PMT = Aunoa
Whakaputanga 1. TuriWheel = Aunoa
Nga tikanga Chromatographic:
Tīwae Chromatographic:
Thermo Scientific Hypersil GOLD C18, 5 um, 100 x 2.1 mm Nama 25005-102130
Wāhi pūkoro:
Rahi tauira: 20 mL
Te tere tere: 800 mL / min
Te pāmahana o te tīwae: 25 C
SPECTRUM MAHI
Fig. 1 Tauira whakatauira chromatogram (1 ppb me te chromatogram paerewa; 2 tauira waatea chromatogram)
I roto i tenei pepa, Aflatoxin B1, B2, G1, G2, M1 i roto i te sesame i tirohia ma te whakamahi i te Thermo Scientific Hypersil GOLD C18, 5 microns, 100 * 2.1 mm te pou, te whakahekenga o te pou i mua i te taha o te kaitautoko. He pai nga tihi, te wehenga me te tino kitenga o nga kitenga i kitea.
Mo nga kete rapu kitenga mycotoxin atu, paatohia ki konei.