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immunochromatography

Me pehea te whakawhanake i te whakamatautau tere i runga i te immuno-chromatography?

Posted i runga i  Mahuru 11, 2020, Etita e Jason, Kāwai  

Tere Tere
He aha te immunochromatography?
Ko te kaupapa o te immunochromatography
Ko te tikanga whakamatautau i te immunochromatography
Nga matūriki karakara e whakamahia ana i te immunochromatography
He aha te koura koroi?
Ko nga momo momo momo whakamatautau e toru i runga i te immunochromatography koura koroke
Nga rauemi mata e whakamahia ana i te whanaketanga immunochromatography
Nga taputapu e whakamahia ana i te whanaketanga immunochromatography
Ko te mahinga hanga i te whanaketanga immunochromatography
Me pehea te whakamahi i te immunochromatography koura colloidal hei kitenga mo te ahuru kai?
Opaniraa

He aha te immunochromatography?

Immunochromatography (immu-nochromatography assay, ICA) he momo hou mo te tikanga immunoassay i puta i nga tau moata o te 1980. He immunoassay ngawari me te nohopuku i whakapumautia i runga i te kaupapa o te whakaoranga (IFA).

Ko te kaupapa o te immunochromatography

Ko te kaupapa o te immunochromatography ko te whakatika i tetahi antibody motuhake ki tetahi rohe o te membrane nitrocellulose. Ina rukuhia te pito nitrocellulose maroke ki roto i te tauira (analyte), na te mahi a te kapi, ka neke whakamua te tauira i te taha o te kiriuhi, ina neke ana ki te waahi e mau ai te antibody, ko te antigen e rite ana i roto i te tauira ka herea ki te antibody . Mena kua pangia ki te immunogold, immunoenzyme ranei, ka taea e te waahi te whakaatu i te roopu tae hei whakatutuki i te immunodiagnosis motuhake.

Ko te tikanga whakamatautau i te immunochromatography

Radioimmunoassay (RIA)

Whakamahia ai e te hangarau RIA nga antigens, nga antibodies ranei kua tapaina ki nga isotop radioactive (penei i te 125I, zhi32P, 3H, me etahi atu) ki te mehua i te irirangi o nga γ-hihi, β-hihi ranei me te kaitohu γdao-ray rama ranei ki te taatai ​​waipiro hei whakatau i nga aukati, i te rahinga antigen ranei hangarau. Kei roto ko te radioimmunoassay e whakaatuhia ana e te antigen tapanga me te tirotiro i te mate Immoradiometric (IRMA) e whakaatuhia ana e te tohu antibody. Ko te mea o mua e whakamahi ana i te waahanga wero whakataetae tikanga here, e ine ana i nga antigens macromolecular me nga antigens rāpoi ngota iti; ko te whakamutunga e whakamahi ana i te tikanga-totoka hei ine i nga antigens macromolecular.

Ko te RIA e noho ana i te waahanga nui i roto i nga tikanga immunoassay pesticide whakatikatika wawe, a ka whakaputahia te hihi o te dieldrin, aldrin, 2,4-D me 2,4,5-T, parathion me paraquat. Immunoassay. Ahakoa he tairongo tenei tikanga (ko te RIA he 10-9g, 10-12g, tae atu ki te 10-15g) a he maha nga momo tono, me kii nga kaute utu nui ki te mahi i te RIA, a kei kona ano etahi raru penei i te radiation me te poke. . Ko te whakamahinga me te whakawhanaketanga o te waahanga kitenga ka tutuki i etahi herenga, ka mutu ka whakakapihia e etahi atu tikanga immunoassay.

Enuno immunoassay (enzymeimmunoassay, EIA)

EIA he hangarau immunoassay whakawhanakehia i muri i te RIA. Ko te kaupapa rapunga he rite ki te radioimmunoassay, engari ko te tapanga e whakamahia ana he hauropi, e whakakotahihia ana e te rauropi te uruparenga aukati motuhake o nga antigens me nga antibodies me te tino kaha o te haurangi o te haurangi. Ko te ngohe o te whākōkī herea ki te wahanga totoka ka inea ki te whakatau i te Ine te rahi o te matū. Ko nga haurangi e whakamahia ana hei tohu he horseradish peroxidase (HRP) me te alkaline phosphatase (AKP), glucose oxidase (GO), urease (urease) me era atu.

Ko te tautoko pakari mo te uruparenga tapanga whākōkī he ngongo kirihou polystyrene me ngā kiriuhi. I tenei wa, ko te nuinga o nga pereti microtiter 96-pai (MTP) e whakamahia ana hei tautoko pakari. Ko tenei momo pereti he nui te kaha kitenga me te maha o nga tauira, ana ko te kaiparau miihini ngawari noa iho ka taea te whiwhi raraunga kitenga tika. Ko etahi o nga maatauranga e whakamahi ana i nga peara aukume hei rauemi waahanga totika mo te rangahau EIA. Ko te kaupapa ko te takai i nga rauemi polymer (polystyrene, polyvinyl chloride, me etahi atu) ki waho o nga pata iti o te konganuku (Fe2O3, Fe3O4), katahi ka hono ki nga roopu amino. (-NH2), carboxyl (-COOH), hydroxyl (-OH) me etahi atu roopu kaha e honoa ana ki nga paturopi, ki nga antigens ranei hei hanga i nga peepi aarai. Ko nga mea pai o tenei tikanga ko te microbeads he nui te waahanga o te mata, he kaha te whakaputa adsorption, a ka taea te whakamutua i roto i te waahanga wai kia tere te hopu i te wetewete i te tauira. I muri i te whakamahinga o te papa aukume, ka taea te wehe wawe i nga microbeads mai i te otinga tauira, na reira ka iti ake te kitenga. Te wa, whakapai ake i te mohio o te kitenga.

Na te mea he ngawari ki te whakarite, pumau, me te kore utu nga reagapanga reimana-ingoa, me te mohio ki te immunoassay o te whākōkī e tata ana ki te hangarau radioimmune, kua tere te whanake o te hangarau EIA i roto i enei tau kua hipa, a kua whanaketia nga momo tikanga EIA. I waenga i a ratau, ko te whakawairakau imono-honohono hono-hono (te tiiwhakaaro imeakorakewhakaaro, ELISA) koinei te tikanga immunoassay whanui e whakamahia whanui ana i roto i te kitenga toenga pesticide.

Korewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewharewhare

Ko te maataapono maamaa o te kitenga FIA ko te whakakotahi i te tino motuhake o nga antigens me nga wauropi me te inenga taumaha o te karawhiu, me te whakamahi i nga matū huatau hei kaiwhakamahara hei tapa i nga paturopi, antigens, ngota hapten ranei hei whakarite i nga reagents fluorescent tino kounga kairangi. I te wa e whitikihia ana te raukikini i roto i te antigen-antibody conjugate e te rama ultraviolet me te marama kikorangi ranei, ka taea te mimiti i te kaha o te marama ka uru ki te ahua hihiri. Ka hoki mai ano ki te papa o te whenua mai i te ahua harikoa, ka taea te whakaputa i te kaha o te marama i roto i te hiko hiko hiko, ka hua ake ai te hinu. Ko te whakamahere i te pehanga o te pesticide pungarehu-kaha ki te tohu i te toenga pesticide i roto i nga tauira.

Ko te Fluorescein e tika ana mo te tapanga i nga paturopi, antigens, ngota hapten ranei, me tutuki nga whakaritenga:

  1. Me whai roopu matū e taea ai te hono hononga totika me te ngota ngota o te pūmua, kia ngawari ranei te huri ki tenei momo tauhohenga me te kore e whakangaro i te hanganga o te rerenga;
  2. Whai muri i te waitohu, kaore e rereke te hanganga matū me nga taonga o te fluorescein me te antibody antigen ranei;
  3. He nui te kaha o te fluorescence, ana ko te whakaritenga mo te herea me te poroteine ​​he iti;
  4. He ngawari, he tere hoki te mahinga here heru ki te poroteini, ana ka ngawari te tango i te fluorescein koreutu me ona hua whakahekeheke;
  5. He pumau te mahi a te kaitautoko i raro i nga tikanga mo te rokiroki whanui ka taea te penapena mo tetahi wa roa.

Chemiluminescence immunoassay (luminescentimmunoassay, LCIA)

Ka taea te wehewehe i te LCIA ki te immunoassay chemiluminescent (immunoassay chemiluminescent, CLCIA) me te immunoassay koiora-marama (immunoassay koiora-marama, BLCIA).

I te 1976, i whakapumautia tuatahihia e Shroeder tetahi hangarau immunoassay koretake hei whakamahi i te punaha biotin (B) -avidin (A). Muri iho, ka whakawhitihia e Halman raua ko Velan ki nga punaha heterogeneous, kua uru nei ki te rangahau koiora. Nga momo mara. Ko te kaupapa hei tohu i te huinga o antigen me te antibody mā te māramarama. Ka honoa te tapanga maarama me te antibody e tika ana, te antigen ranei, ka tauhohe te wehenga me te haurangi, te urupare redox ranei me te kaihoko rama, te waikore ranei (penei i te rubrene, me etahi atu.) Whakaohooho me te tuku i te kaha marama. I te mutunga, i whangangahia te kaha whiti me te whakaahua mo te taatai ​​rahinga. Ko nga tohu luminescent e whakamahia ana he horseradish peroxidase (HRP), luminol (luminol), isoluminol (isoluminol), lophine (lophine), lucigen (lucigen), rua (2, 4, 6) -Trichlorobenzene) oxalate, biphenyltriol me 6 [N- (4-diaminobutyl) -N-ethyl] -amino-2,3-dihydrophenazine-1,4-dione (ABEI) me etahi atu. Ko te antibody (antigen ranei) kua tohua ki te tohu luminescent kua whakahuatia i runga ake nei me nga antigen e hangai ana. (he antibody ranei) i roto i tetahi otinga buffer pH, ka tukuna e ia he maama i raro i te mahi a te mea mahi tahi (penei i te H2O2, me etahi atu), me te kaha o te tiimatanga ka orite ki te waahanga whakamatautau. He haangai ki te kukume, no reira ka taea te whakamahi mo te taatai ​​rahinga.

Luminescence immunoassay he painga o te tino kiko, he tairongo nui (rohe kitenga o te 10-15mol / L), tere (1 ~ 3h), me te waatea ngawari o nga taonga luminescent. Heoi, ko tona mahinga whakamarama me tona kaha ka pangia e te hanga matū o te taonga e whiti ana, te pH o te reo, te taonga honohono me nga poke katote maitai.

Nga whakamatautau koura immunochromatography (whakamatautau kouraimmuno-chromatography, GICA)

Ko te maataapono o te kitenga GICA ko te whakakakahu i nga kaanawa (paturopi antigens ranei) ki runga i te membrane microporous penei i nga membrane nitrocellulose i te ahua raina. Ko te tapanga koura colloidal ka mau ano ki nga taana, ki etahi atu mea ranei i te ahua maroke i runga i nga taonga mimiti. Ma te mahi capillary e kauhoe ai te otinga tauira ki runga i te papa chromatographic. Ka kauhoe ana ki te tohu koura colloidal, mena kei roto i te tauira te kaiwhiwhi hei whakamatautau, ko te mahi tuatahi he tino whakautu aukati ki te hanga i tetahi matatini aukati Ina kaukau tonu te taonga ki te rohe paninga raina, te tuarua o nga waahanga ka puta he urupare aukati, ka mau te matatini aukati ki te rohe raina pani, ka whakaatuhia te roopu whero e te koura colloidal (Roopu kitenga), ka whiti te tapanga koreutu i te roopu rapunga ka wehe motuhake i te tapanga here . Ko te whakatau kohinga, ko te rahinga ranei o te whakatau ka tutuki ma te kimi i te aroaro, i te ngaro ranei o te tae, i te atarangi o te tae i runga i te whitiki2.

Kitea ana e te Kete Whakamatau Kete Koura

Ko te tikanga GICA he painga no te nohopuku (5-20min), he iti, he maama nga hua, kaore he pukenga mahi uaua me nga taputapu motuhake, he ngawari ki te kawe. Heoi, ki te whakaritea ki etahi atu tikanga immunoassay, he iti iho te kitenga o tenei tikanga, a he pai ake mo te waahanga tere tere-whakatau ranei i te waahanga-haangai. I tenei wa, kua whakamahia tenei tikanga i roto i nga waahanga rangahau penei i te rongoa me te koiora, a he nui te whakamahi i nga whenua whanake.

Hangarau honohono o te immunoassay me te hangarau taatai ​​taputapu

Ko te whakamahinga o tetahi tikanga IA hei tarai i nga toenga pesticide he iti nei nga korero, a he iti rawa te kowhiri i nga tikanga taatai ​​me te taatai ​​matū. Kramer et al. i whakamahia nga immunoassays me te chromatography wai (LC) hei whakakotahi, ma reira ka ngawari te tikanga wetewete me te whakapai ake i te tino kitenga. Ko te whakakotahi o te LC-IA e hono ana i te kaha wehe o te LC me te tino awangawanga me te tino motuhake o IA. Ko tenei tikanga taatai ​​he pai rawa atu mo te taatai ​​toenga maha-waahanga me te microanalysis. Ko te whakakotahitanga o te immunoassay me te chromatography hau, te spectrometry papatipu (GC MS) ranei ka taea te whakaiti i nga tauhohenga whakawhiti i te taatai ​​i nga pesticides me nga metabolite me nga hanganga rite ki te whakaiti i nga kino teka.

Nga matūriki karakara e whakamahia ana i te immunochromatography

Nanoparticle koura Colloidal
Matūriki Latex
Wahi korakora
Pirepire aukume
Waikano Fluorescent
Tohu rita
Maarama hurihuri

He aha te koura koroi?

colloidal-koura-korakora
colloidal-koura-korakora

Koura Koura, e mohiotia ana ko nga nanoparticles koura, he turanga pumau, whakakakahu, me te whakamararatia takitahi o nga korakora koura e tarewatia ana i roto i te wai i muri i te toenga o te tote koura ki te koura. Ko nga matūriki koura he mauri koura me te papanga katote takirua e karapoti ana i a ia.

Ko te tae o te otinga ka whakawhirinaki ki te tae o nga waahanga waahanga marara, te marara o nga waahanga waahanga marara, me te momo maamahi ohorere, ahakoa he marara marara, he rama kua whakawhiti ranei. Ko te iti o nga korakora me te teitei o te marara, ko te poto o te roangaru o te rama marara. Mo te hydrosol o taua mea ano, he rereke te rahi o te korakora, he rereke ano te tae.

Hei tauira, ko nga korakora koura korowha te rahi i waenga i te 5-20 nm, te roangaru o te ngongo o te 520 nm, he tae waina whero; ko te rahinga i waenga i te 20-40 nm te nuinga ka mimiti i te rama matomato me te roangaru o te 530 nm, ana he whero pouri te otinga; ko te 60 nm te korakora koura korara ka ngongo i te roangaru 600 nm te karaka karaka, ko te otinga he kikorangi-puru. Ko nga korakora koura kapi e whakamahia ana i te waimarie ki te 5-60 nm, a he whero te otinga.

Ko nga momo momo momo whakamatautau e toru i runga i te immunochromatography koura koroke

Ko te korakora karakara rongonui ko te nanoparticle koura. Ko te hangarau koura Colloidal e hono ana i te urupare aukati antigen-antibody me te hangarau tapanga koura koroidal mo te kitenga kounga me te rahinga o te antigen me te ihirangi antibody. Na nga mea pai penei i te tere, ngawari, iti te utu me te pai o te pumau, kua kitea te whakamahi koura koki i roto i nga mahi haumanu haumanu.

I tenei wa, ko nga tikanga rapunga e whakamahia ana mo nga waahi koura koura kitea ko te tikanga hanawiti-antibody rua, te tikanga whakataetae me te tikanga koretake. He nui nga rereketanga i waenga i te tikanga whakamatautau me nga taatai.

Tikanga Sandwich Antibody Taarua-

Ko te tikanga hanawiti taawewe-rua ko te tikanga rapunga e tino whakamahia ana mo nga papa kimi koura koroke, e whakamahia ana hei kimi i nga koiora koiora me nga antigens korakora. Ko te tikanga hanawiti taawewe-takirua me whakarite te antibody takirua o te antigen hei whakamatautau. Ko tetahi antibody e tapaina ana ki te koura colloidal ka mau ki runga i te papa whakakotahi, a ko tetahi atu antibody e mau ana ki te raina rapunga (raina T) o te membrane NC. Hei taapiri, he mea tika ki te whakarite i tetahi hauropi tuarua e tino hono ana ki te rauropi tapaina koura (arā, te rauropi tohu-koura colloidal) ka whakatika ki te raina whakahaere (raina C) o te membrane NC.

lateral-rere-immunoassay
lateral-rere-immunoassay
taha-rere-Unuhia

Ka oti ana te tauira te taapiri ki te riu, ka nuku ki te papa tauira, papa whakakotahi, raina T, raina C me te papa mimiti (papa wicking) i roto i te raupapa ma te kaupapa rere rere capillary. Ko nga tauhohenga o ia atamira e whakaaturia ana i te ripanga e whai ake nei:

 Neke tauira T papa whakawhitiTauira neke ki te raina TNeke te tauira ki te raina Chua
Kei te Anaylte te antigenHe antigen i tapaina i te koura i mau i te antigen, ka waiho hei uauaKo te paturopi o te raina T ka mau i te matatini, ka kitea te peera taeKa hopuhia e te antibody tuarua te antibody tapanga-koura me te peera tae e kitea anaE rua raina e kitea ana
Anaylte kaore he antigenKaore he urupareKaore he huringa taeHe rite i rungaRaina C anake e kitea ana

No reira, kia rua nga raina whero e whakaatuhia ana ki te hihi, ko te tikanga he pai nga taonga hei whakamatautau i te tauira; ina he raina C anake kei runga i te Unuhia, ko te tikanga ko nga taonga hei whakamatautau i te tauira he kino. Ko te nui o te tarukino ka kaha ake te tae o te raina-T.

Tikanga Whakataetae

He uaua nga antigens ngota iti hei whakarite i nga paturopi takirua (te taumaha o te ngota ngota, he uaua te kimi i nga waahi herea kia rua nga paturopi hei here i te wa kotahi), no reira kaore e kitea nga antigens ngota iti e te tikanga hanawiti antibody rua. Mo nga antigens ngota iti, whakamahia te tikanga whakataetae hei whakamatautau.

I roto i te tikanga whakataetae, ko te antibody tapanga-koura kua mau ki te papa cojugate o, ana ko te raina T i runga i te membrane NC me nga antigen honohono macromolecular hei whakamatautau (kaore e taea te whakatika tika i nga antigen iti ngota ki te membrane NC. Na reira , he mea tika kia honohono i te ngota ngota iti ki te BSA me etahi atu matū macromolecular ka whakakakahu ai ki te membrane NC). Ko te antibody tuarua e ahei ana ki te hono ki te rauropi tohu-koura kua whakakakahuihia ki te raina C.

taha-rere-rere-whakamatau-whanaketanga
taha-rere-rere-whakamatau-whanaketanga
taputapu-taha-rere
taputapu-taha-rere

Ka oti ana te tauira te taapiri ki te riu, ka nuku ki te papa tauira, papa whakakotahi, raina T, raina C me te papa mimiti (papa wicking) i roto i te raupapa ma te kaupapa rere rere capillary. Ko nga tauhohenga o ia atamira e whakaaturia ana i te ripanga e whai ake nei:

 Neke tauira T papa whakawhitiTauira neke ki te raina TNeke te tauira ki te raina Chua
Kei te Anaylte te antigenHe antigen i tapaina i te koura i mau i te antigen, ka waiho hei uauaKaore e taea te hopu antigen. Kaore he roopu taeKa hopuhia e te antibody tuarua te antibody tapanga-koura me te peera tae e kitea anaRaina C anake e kitea ana
Anaylte kaore he antigenKaore he urupareHopukia te tohu antibody me te roopu tae tae-kouraHe rite i rungaE rua raina e kitea ana

No reira, kia rua nga raina whero e whakaatuhia ana ki te Unuhia, te tikanga ko nga mea hei whakamatautau i te tauira he kino; ina he raina C noa kei runga i te Unuhia, ko te tikanga he pai nga taonga hei whakamatautau i te tauira. Ko te nui o te tarukino ka kaha ake te tae o te raina-T.

Tikanga Hono

Ko te tikanga autaki e whakamahia ana kia kitea nga paturopi. Mena he tikanga koretake, ka whakapirihia te papa whakakotahi ki te pūmua A kua tapaina ki te koura colloidal (Ka taea e te Protein A te hono atu ki te antibody kore-motuhake). Ko te raina T i runga i te membrane NC kua mau ki te antigen e ahei ana te hono ki te rauropi kia whakamatautauria, a ko te raina C i runga i te membrane NC me te anti-Protein A.

whakamātautau-immunochromatographic
whakamātautau-immunochromatographic
taha-rere-Unuhia

Ka oti ana te tauira te taapiri ki te riu, ka nuku ki te papa tauira, papa whakakotahi, raina T, raina C me te papa mimiti (papa wicking) i roto i te raupapa ma te kaupapa rere rere capillary. Ko nga tauhohenga o ia atamira e whakaaturia ana i te ripanga e whai ake nei:

 Neke tauira T papa whakawhitiTauira neke ki te raina TNeke te tauira ki te raina Chua
Kei roto i te Anaylte te aukatiPūmua Tapanga-koura He hauropi kua mau, kua uauaAntigen hopu antibody, kara tae e kitea anaPūmua A i kapohia e te anti-proten He paturopi me te peera tae e kitea anaE rua raina e kitea ana
Anaylte kaore he antibodyKaore he urupareKaore e taea e Antigen te hopu antigen, kaore he tae e rerekeKo te antibody tapaina koura i hopuhia e te antibody tuarua, he tae tae e kitea anaRaina C anake e kitea ana

No reira, kia rua nga raina whero e whakaatuhia ana ki te hihi, ko te tikanga he pai nga taonga hei whakamatautau i te tauira; ina he raina C anake kei runga i te Unuhia, ko te tikanga ko nga taonga hei whakamatautau i te tauira he kino. Ko te nui o te tarukino ka kaha ake te tae o te raina-T.

Nga rauemi mata e whakamahia ana i te whanaketanga immunochromatography

  1. Antigen me te paturopi
  2. Albumin Serum Bovine
  3. Te rere o te kaari o muri
  4. Membrane Nitrocellulose
  5. Papa tauira (Papa Wicking)
  6. Pad Conjugate
  7. Papa mimiti
  8. Membrane wehenga toto (whiringa)
  9. Kete Kaseti (kōwhiringa)

Nga taputapu e whakamahia ana i te whanaketanga immunochromatography

  1. Punaha tohatoha Robotic (Kaituku)
  2. He tapahi tarai mo te riipene whakamatautau rere taha
  3. Maikorero
  4. Miihini whakatio mīti
  5. Te oumu werawera
  6. He tere tere mo te porotita
  7. Punaha whakahaere Liquid (whiringa)
  8. Tukatuka keehi keehi (waahanga)

Ko te mahinga hanga i te whanaketanga immunochromatography

  1. Kua oti te koura Colloidal
  2. Tapanga koura Colloidal
  3. Te tuku antigen, antibody ranei ki Membrane Nitrocellulose
  4. Nitrocellulose membrane piri ki kāri tautoko
  5. Laminate papa whakapiri i runga i te kaari tautoko
  6. Laminate tauira papa i runga i te kaari tautoko
  7. Laminate papa mimiti i runga i te kaari tautoko
  8. Tapanga Moko Piri ki te papa mimiti (whiringa)
  9. Te tapahi i te pepa kaore i tapahia ki roto ki nga takai
  10. Whakahauhia nga takai ki roto ki te peeke tarai pounamu ranei
  11. Te pikau i te pouaka

He korero taapiri, tirohia Me Pehea Te Whakawhanake i te Whakamatau Tere i te Tere Tere?
Me pehea te whakamana i te huihuinga mo te whakamatautau tere?

Me pehea te whakamahi i te immunochromatography koura colloidal mo te haumaru kai kitenga?

Immunochromatography koura Colloidal whakamahia whānuitia ai i te whakamātautau kai mō ngā tau maha. Hei tauira, me pehea te whakamātautau miraka mahi i nga toenga paturopi i te miraka kau?

Ko te raukati paturopi he ngota iti, no reira, ka whiriwhiria e te kaimanaaki te tikanga whakataetae. He aha te tikanga tauhohenga?

Kia kite a i te hanganga Unuhia:

lateral-flow-assay
lateral-flow-assay
nanoparticles koura
nanoparticles koura

Ko te koura colloidal tapanga antibody lyophilizated i roto i pereti perehana. Wehenga mai i te Unuhia, whakapiki i te waa whakaohooho me te whakarei ake i te mohio o te kitenga.

He mahinga tauhohenga rere a-taha

hangarau-taha-rere-hangarau
hangarau-taha-rere-hangarau
immunochromatography
immunochromatography

I Tauira Pai:

  1. Tangohia he tauira miraka noa ka whakauru ki nga puna moroiti
  2. Mena he miraka iti te hinu miraka, penei i te beta lactam, tetracycline, chloramphenicol, Sulfonamides, Cephalosporins, Melamine, etc.
  3. Ko te antibody tapaina koura (Au-Ab) kei roto i nga puna moroiti ka mau i nga ngota iti mai i nga tauira miraka, ka waiho hei matatini hou (Au-Ab e herea ana ki te ngota iti).
  4. Ahakoa ka neke te uaua ki te tūranga raina T, kaore he tuunga mo te BSA-antigen (te antigen i piri ki te T lin) ki te whakakotahi, na te mea kua whakakotahihia te nanoparticle koura me nga ngota iti, na, kaore e rereke te peera tae.
  5. Ko te paturopi tuarua i whakapauhia ki te raina C ka mau ki te rauropi tohu-koura me te peera tae e kitea ana.
  6. Ka pai te mutunga.

I roto i te Tauira Kino:

  1. Kaore e taea e te antibody tapanga koura (Au-Ab) te hopu i nga ngota iti mai i te miraka noa, engari ka mau i te antigen o te raina T, katahi ka kitea te peene tae.
  2. Ko te rauropi secodary o te raina C kei te mau tonu te hauropi tapaina koura (Au-Ab), he peera tae e kitea ana.

Te whakamaoritanga

Ko te tikanga Colorimetric mo te whakamaori i te whakamatautau miraka. Ka whakataurite te raina whakamatautau ki te raina whakahaere hei whakatau i te hua pai, i te kino ranei. I a koe e mahi ana hei whakahaere i te maha o Au-Ab kia tutuki ai te whakamatautau i te tae.

Opaniraa

Immunochromatography he hangarau hangarau whakamatautau tino rongonui, e whakamahia whanui ana i nga hua taatai ​​in vitro, kaore i te whakamahia noa mo te whakamatautau kai. Motuhake i roto i nga waahanga rerenga o te taha, ko te immunochromatography koura kaore e hiahiatia he taputapu utu nui, hei whakamahi, he utu iti ake hei awhina i te tangata kia ora pai ake, hei tauira, te whakamatautau haputanga i te kaainga.

tohutoro:

Me Pehea te tiimata i te Hanga Whakamaatau Kowai 19 Kete Tiwhikete

Whakairinga tata

NGARU KaiTE WHAKAMAHI MYCOTOXINPANUITANGA PESTICIDEWHAKAPONO TUPUNAWHAKAMAHI KAUPAPAWHAKAMAHI I TE EGGTE HUAKI HUAKI
Whakapehapeha hangaia e BALLYA
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