Nanoparticle koura hei hoko
Ingoa Hua: BALLYA Gold Nanoparticles (Mahinga Nui Rawa Nanoparticle koura)
Rahinga: 20nm ~ 160nm
Ingoa: Nanoparticles koura + wai ma
Whakauru: 100 OD (± 5OD)
Whakaritea: 100 OD 200 XNUMX ranei
Tuhinga o te Rahi Nanoparticles Gold
Whakamahia te Kaituri Rahinga Whaiti hei taatai i te rite o nanoparticles koura rahinga Mena he iti ake te wariu o te PDI i te 0.2, na te tikanga he orite nanoparticles koura rahi. Anei e toru te rahi o BALLYA Koura Nanoparticle te rahi kei nga ahua o raro nei me te 40nm (PDI: 0.067), 50nm (PDI: 0.121) me te 60nm (PDI: 0.094).
Te Maatari Matapihi Taatari Matapihi Tuhuri (Ka iti ake te PDI i te 0.2)
| Te nama take | rahi matūriki | Rahinga toharite |
| 60-0.1 (10x waimeha) | 67.31 | 66.81 | 66.38 | 66.83 |
| 60-0.05 (20x waimeha) | 65.73 | 65.19 | 65.24 | 65.39 |
| 60-0.02 (50x waimeha) | 68.19 | 68.03 | 67.90 | 68.04 |
| 50-0.1 (10x waimeha) | 56.59 | 56.27 | 56.17 | 56.34 |
| 50-0.05 (20x waimeha) | 56.81 | 56.94 | 56.58 | 56.78 |
| 50-0.02 (50x waimeha) | 53.86 | 53.32 | 53.54 | 53.57 |
| Te nama take | POI | PDI toharite |
| 60-0.1 (10x waimeha) | 0.153 | 0.156 | 0.148 | 0.152 |
| 60-0.05 (20x waimeha) | 0.171 | 0.165 | 0.165 | 0.167 |
| 60-0.02 (50x waimeha) | 0.150 | 0.164 | 0.147 | 0.154 |
| 50-0.1 (10x waimeha) | 0.199 | 0.198 | 0.190 | 0.196 |
| 50-0.05 (20x waimeha) | 0.191 | 0.185 | 0.185 | 0.187 |
| 50-0.02 (50x waimeha) | 0.182 | 0.187 | 0.203 | 0.191 |
Hanga Nanoparticles koura
| Nanoparticles koura noa | BALLYA Koura Nanoparticle |
| Hanga rite | Porohita te hanga | Porohita te hanga |
Tapatoru \ Rhombus \ Torotika
Oval \ Tapawha \ Korekore |
Ko nga nanoparticles ahua koretake ka nui te tango i roto i te waikawa koura koroi. Ma tenei ka tau:
1. He ngawari ki te mate
2. He uaua ki te pani ki te antibody / antigen / protein
3. Tangohia te nui o te antibody / antigen / protein i te waitohu
4. Tangohia te nui o te rahi o te nanoparticles i te waitohu
5. Parataiao
6. Whakakotahitanga | Porowhita te ahua neke atu i te 95%
CV iti iho i te 8%
|
Whakataurite Nanoparticle koura
| tūemi | Nanoparticles koura noa | BALLYA Koura Nanoparticle |
|  | |
| Nanoparticles koura mate | Āe | No |
| Whakaaetanga | Āe | No |
| Whakapapa | Āe | No |
| Te whakanui ake i nga taonga tapanga | Āe | No |
| Te whakaiti rauemi tapanga | No | Ae (max whakaora 80%) |
| Te kukū nui | Kao (1 OD - 20 OD) | Ae (100 OD - 200 OD) |
| Te whakamahinga o nga nanoparticle koura | nui | iti |
| Tuhinga | Kaore i roto | Kaore i roto |
| Te waitohu i te wa | roa | poto |
| pūmautanga | marama 1-6 | marama 12 |
| Te kaha hanga | 1-30L | 100L i te wa kotahi |
| Riterite roopu | iti | Tino teitei |
| Hare whakairi | Tino Hāngai | iti |
| Whakanuia te maarama | No | Āe |
| pāmahana Storage | tohu 4-8 | Utu pāmahana rūma, penapena 4-8 tohu |
Nga Painga o te BALLYA Gold Nanoparticles
Monodisperse Gold Nanoparticles
Tikanga whakaputa hou: nanoparticles koura kia porohita te hanga> 95%; teitei monodisperse me pupuri i te 'tawhiti hapori' i waenga i nga nanoparticles; A ape i te whakakao me te parataiao
Whakatika: He nanoparticles ahua koretake i raro iho i te 5%, CV% iti iho i te 8%
Te pumau me te ora papa: marama 12
Tikanga whakaputa puranga: Te mahi whakahiato Max 100L i te wa kotahi, te whakaheke i te whakarea o te rereketanga (CV) i waenga i nga puranga
Nga painga o nga BALLYA Gold Nanoparticles
1. Kaore i mate nanoparticles koura
2. Kaore he whakakao
3. Kaore he Parataiao
4.> 95% porowhita te hanga nanoparticles koura
5. CV% Iti: iti iho i te 8%
6. Te whakanui i te mohio
7. Te whakanui ake i te motuhake
8. Te whakanui ake i te mahinga o te hua oti me te noho rite
9. Te roopu tae i te wherikotanga teitei me te maama
10. Kaore he pakaru i te roopu tae
11. 100L mo ia whakangao puranga, awhina i a koe ki te whakaheke i te CV% i waenga i nga waahanga rereke o nanoparticles koura
12. Penapena kia 80% nga rauemi tapanga (antigen, antibody, protein)
Ka aha i muri i te whakamahinga o te BALLYA Gold Nanoparticles?
Nanoparticles Koura Nui Te Kaha Rawa
1. Te rahinga whakaheke tae atu ki te 80% o nga taonga tapanga
Te arotake i te hanga 10,000
koura koura whakamātautau tere mo te kirika poaka African
| Te nama take | Nanoparticles koura noa | BALLYA Koura Nanoparticle |
| Nga rauemi tapanga | 5mg | 2mg |
| Te whakamahinga o nga nanoparticle koura | 1500ml (1 OD) | 37.5ml (40 OD) |
| Te wa mahi | 1 ra | 4h |
| Te wa whakaputa | 4 ra | 3 ra |
parau: i runga ake i te papa kua whakarerekehia kia rite ki te ahua whakaputa me te tikanga, hei tohu noa iho!
Te aromātai i te mahi whakangao koura koura whakamātautau tere mo te HIV
| Rauemi Tapanga | Rōpū tapanga (ug / ml) | Tiaki |
| 1 OD nanoparticles koura | 40 OD nanoparticles koura |
| HIV Antigen 1 | 2.6 | 1.2 | 53.85 orau |
| HIV Antigen 2 | 1.6 | 1.2 | 25 orau |
| HBsAg Antibody | 5.8 | 1.1 | 81.03 orau |
parau: i runga ake i te papa kua whakarerekehia kia rite ki te ahua whakaputa me te tikanga, hei tohu noa iho!
Te aromātai i te mahi whakangao koura koura covid 19 whakamatautau tere
| Rauemi Tapanga | Rōpū tapanga (ug / ml) | Tiaki |
| 1OD nanoparticles koura | 40OD nanoparticles koura |
Antigen SARS-CoV-2
ASAR-27 | 2.8 | 1.3 | 53.57 orau |
Antigen SARS-CoV-2
ASAR-28
| 3.2 | 1.5 | 53.13 orau |
parau: i runga ake i te papa kua whakarerekehia kia rite ki te ahua whakaputa me te tikanga, hei tohu noa iho!
2. Penapenahia nga rauemi a te tangata me te waitohu
3. Whakapai ake i te hua
4. Whakamahia te centrifuge Papamahi kia pai ai te mahi papatipu
5. Whakaitihia te puranga CV%, kia mau ki nga hua kia rite
6. Poto te huringa whakawhanaketanga 50% pea
Nga momo nanoparticle koura BALLYA
| Ngeru. Kare. | Rahi (nm) | Whakauru | mōkī |
| BGN00040 | 40 | 100OD | 10ml - 1000ml |
| BGN00050 | 50 |
| BGN00060 | 60 |
| BGN00070 | 70 |
Ota BALLYA Koura Nanoparticles
Nga Paatai Auau me nga Whakautu
Me pehea te whakamahi i nga BALLYA Gold Nanoparticles?
1. Whakaritea te otinga buffer a PH tika rawa atu
2. Taapirihia te otinga buffer ki te 100 OD nanoparticles koura waimeha tae noa ki te 40 OD
3. Taapirihia he pūmua ki te 40 OD nanoparticles koura wai ki te tapanga
He aha te taunakitanga o te tapanga tapanga?
40 OD. I tenei kukume, ka awhina i te whakanui i te maaramatanga, te penapena i nga rauemi tapanga, te whakarei ake i te tae tae o te peeke.
He aha te rahinga tapanga pai rawa atu?
Ka whakawhirinaki atu ki to mahinga whakaputa me to momo hua, penei i te, hcg, hiv, covid 19 ... etc. He rereke nga mea katoa. Me mahi rangahau.
Me pehea te hanga i te otinga buffer me te PH e tika ana?
Tirohia koa nga paearu o te wairewa buffer.
Me pehea te rapu i te PH tino tika i roto i to punaha hangarau?
1. 100 OD kukume o koura koura, waimeha ki te 40 OD me te rongoa rereke rereke pH
2. Taapirihia he taua kounga antibody (e taunaki ana 50 ug / ml), tauhohe 2 haora
3. I te otinga o te urupare, tangohia he iti o te koura colloidal tapanga, ka rewa ki te 1 OD me te pH e taurite ana.
4. I roto i te 1 OD tapanga-koura, taapirihia te 10% NaCl kia 1% kukū, tirohia te tae i muri i te whakaranu, ko te tae te mea pai, ko te mea iti rawa iho, kaore ano hoki he kohinga whakahinuhinu, na, koira te pH tino pai
He aha te tauira buffer e tika ana ma BALLYA Gold Nanoparticles?
E 3 nga momo otinga buffer noa i raro nei:
Te whakataunga waipiro e paa ana ki te waikawa (0.01 mol / L)
| pH | 0.01M Waikawa Citric (ml) | 0.01M Trisodium citrate dihydrate (ml) |
| 3.0 | 18.6 | 1.4 |
| 3.6 | 14.9 | 5.1 |
| 4.0 | 13.1 | 6.9 |
| 4.6 | 10.3 | 9.7 |
| 5.0 | 8.2 | 11.8 |
| 5.6 | 5.5 | 14.5 |
| 6.0 | 3.8 | 16.2 |
| 6.6 | 1.4 | 18.6 |
Waikawa Citric: C6H8O7 · H2O: 0.01 mol / L; Wai: 2.101g / L
Trisodium citrate dihydrate: Na3 C6H5O7 · 2H2O ： 0.01mol / L; Wai: 2.941 g / L
Te rongoa aukati e pa ana ki te Alkaline (0.02 mol / L)
| pH | 0.02M borax (ml) | 0.02M waikawa boric (ml) |
| 7.4 | 1.0 | 9.0 |
| 7.6 | 1.5 | 8.5 |
| 7.8 | 2.0 | 8.0 |
| 8.0 | 3.0 | 7.0 |
| 8.2 | 3.5 | 6.5 |
| 8.4 | 4.5 | 5.5 |
| 8.7 | 6.0 | 4.0 |
| 9.0 | 9.05 | 1.05 |
Borax: Na2B4O7 · 10H2O, 0.005M; Wai: 1.907 g / L
Waikawa Boric: H2BO3, 0.02M; Wai: 1.237 g / L
He maamaa te Borax ki te ngaro i te wai tioata, ana me waiho ki roto i te pounamu me te plug.
Rongoā Puka (Citric Acid + borax) (0.01 + 0.05)
| pH | 0.01M Waikawa Citric (ml) | 0.05M borax (ml) |
| 4.4 | 6 | 4 |
| 4.9 | 6.5 | 3.5 |
| 5.5 | 7 | 3 |
| 6.0 | 7.2 | 2.8 |
| 7.8 | 7.5 | 2.5 |
| 8.4 | 8 | 2 |
| 8.8 | 8.7 | 1.3 |
| 9.1 | 9.5 | 0.5 |
Borax: Na2B4O7 · 10H2O, 0.005M; Wai: 1.907 g / L
Waikawa Citric: C6H8O7 · H2O: 0.01 mol / L; Wai: 2.101g / L
He maamaa te Borax ki te ngaro i te wai tioata, ana me waiho ki roto i te pounamu me te plug.
Ko te tikanga tapanga e manakohia ana mo BALLYA Gold Nanoparticles
Tikanga adsorption tinana:
Nga whakaritenga penei i raro:
1. Tohua te pH tino pai mo te pūmua tapanga
2. Tohua te otinga buffer e tika ana
| Rongoā paawewe e hangai ana ki te Alkaline | 0.02M borax + waikawa boric |
| Te whakataunga hukahuka e paa ana ki te waikawa | 0.01M Waikawa Citric + Trisodium citrate dihydrate |
| Rongoā Puka (Citric Acid + borax) | 0.01M Waikawa Citric + 0.05M borax |
3. Waimeha tika mai i te 100 OD ki te 40 OD
4. Taapirihia he pūmua ki te 40 OD nanoparticles koura te wai, te ohorere, te whakaoho ranei kia pai te tohatoha me te urupare 2hours
5. Taapirihia he otinga aukati, penei i te, BSA, OVA, me era atu Hohenga 20 min
6. Centrifuge, tango tipua, waimeha ki te pH tika
Ka taea e taatau te whakamahi i etahi atu tikanga tapanga mo nga BALLYA Gold Nanoparticles?
Ae, ko te tikanga. Ka taea hoki te whakamahi i te tikanga matū hei tapa.
Nga Kai o te BALLYA Gold Nanoparticles
E rua noa nga whakauru:
1. Nanoparticles koura
2. Wai Ma
He aha e kore ai e kitea PH i BALLYA Gold Nanoparticles?
He tino teitei te kukume, 100 OD. He noho taurite tonu, kaore he take mo te ururua i nga pakirehua kia kitea ai. He maama ki te whara i nga uiuinga pH.
He aha te PH o BALLYA Gold Nanoparticles?
He kūpapa tonu. pH = 7
He aha te take ka puta nga nanoparticle koura kua mate i aukati?
He paatai noa. Nga momo waitohu 'BSA kei a raatau ake tikanga ka whakaawe i te mahinga o nanoparticles koura, penei, te aukati i te hunga mate, te tupapaku i te mahinga whakamaroke, te whakaiti i te mohio, te whakapiki i te pai hape, me era atu I roto i tera ke, whakarereke i nga momo waitohu rereke o te aukati i te otinga, te whakamahi ranei i nga kaitautoko polymer kore-protein, penei i te, Polyethylene glycol , etc.
He aha te whakamahi i nga nanoparticle koura nui whakahirahira ka whakaiti i te whai hua o te tapanga?
Te mana'o nei au kei te anga atu koe ki te HOOK PAKANGA (te taumaha o nga paturopi, te antigen me nga pūmua).
Hei tauira, penei i nga wa o mua, me tapa te 1 OD me te 5ug / ml, na, i te wa e whakamahi ana koe i te 40 OD akene ki to whakaaro me whakamahi 5ug / ml x 40 = 200ug / ml. Engari ehara ko te pono. Neke atu i te mano nga wa whakamatautau, i kitea e maatau ko te 50ug / ml anake ka taea te whangai (40 OD) kia makona! Na, whakamatauhia te 50ug / ml, akene he miharo koe.
Nga Whakatupato
Rokiroki me te tuku: kaipuke pāmahana rūma, penapena i te 2- 8 ° C, kaua hei pouaka whakamātao
Whakawhiti: i a koe e whakawhiti ana, e tohatoha ranei, whakamahia he pounamu ma hei karo i nga nanoparticle koura kua mate
ora hounga: marama 12
