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Pepa Korero
Nanoparticle koura hei hoko
Ingoa Hua: BALLYA Gold Nanoparticles (Mahinga Nui Rawa Nanoparticle koura) Rahinga: 20nm ~ 160nm Ingoa:Nanoparticles koura + wai ma Whakauru: 100 OD (± 5OD) Whakaritea: 100 OD 200 XNUMX ranei
Tuhinga o te Rahi Nanoparticles Gold
Whakamahia te Kaituri Rahinga Whaiti hei taatai i te rite o nanoparticles koura rahinga Mena he iti ake te wariu o te PDI i te 0.2, na te tikanga he orite nanoparticles kourarahi. Anei e toru te rahi o BALLYA Koura Nanoparticle te rahi kei nga ahua o raro nei me te 40nm (PDI: 0.067), 50nm (PDI: 0.121) me te 60nm (PDI: 0.094).
Te Maatari Matapihi Taatari Matapihi Tuhuri (Ka iti ake te PDI i te 0.2)
Ko nga nanoparticles ahua koretake ka nui te tango i roto i te waikawa koura koroi. Ma tenei ka tau:
1. He ngawari ki te mate
2. He uaua ki te pani ki te antibody / antigen / protein
3. Tangohia te nui o te antibody / antigen / protein i te waitohu
4. Tangohia te nui o te rahi o te nanoparticles i te waitohu
5. Parataiao
6. Whakakotahitanga
Porowhita te ahua neke atu i te 95%
CV iti iho i te 8%
Whakataurite Nanoparticle koura
tūemi
Nanoparticles koura noa
BALLYA Koura Nanoparticle
Nanoparticles koura mate
Āe
No
Whakaaetanga
Āe
No
Whakapapa
Āe
No
Te whakanui ake i nga taonga tapanga
Āe
No
Te whakaiti rauemi tapanga
No
Ae (max whakaora 80%)
Te kukū nui
Kao (1 OD - 20 OD)
Ae (100 OD - 200 OD)
Te whakamahinga o nga nanoparticle koura
nui
iti
Tuhinga
Kaore i roto
Kaore i roto
Te waitohu i te wa
roa
poto
pūmautanga
marama 1-6
marama 12
Te kaha hanga
1-30L
100L i te wa kotahi
Riterite roopu
iti
Tino teitei
Hare whakairi
Tino Hāngai
iti
Whakanuia te maarama
No
Āe
pāmahana Storage
tohu 4-8
Utu pāmahana rūma, penapena 4-8 tohu
Nga Painga o te BALLYA Gold Nanoparticles
Monodisperse Gold Nanoparticles Tikanga whakaputa hou:nanoparticles koura kia porohita te hanga> 95%; teitei monodisperse me pupuri i te 'tawhiti hapori' i waenga i nga nanoparticles; A ape i te whakakao me te parataiao Whakatika: He nanoparticles ahua koretake i raro iho i te 5%, CV% iti iho i te 8% Te pumau me te ora papa: marama 12 Tikanga whakaputa puranga: Te mahi whakahiato Max 100L i te wa kotahi, te whakaheke i te whakarea o te rereketanga (CV) i waenga i nga puranga
Nga painga o nga BALLYA Gold Nanoparticles
1. Kaore i mate nanoparticles koura 2. Kaore he whakakao 3. Kaore he Parataiao 4.> 95% porowhita te hanga nanoparticles koura 5. CV% Iti: iti iho i te 8% 6. Te whakanui i te mohio 7. Te whakanui ake i te motuhake 8. Te whakanui ake i te mahinga o te hua oti me te noho rite 9. Te roopu tae i te wherikotanga teitei me te maama 10. Kaore he pakaru i te roopu tae 11. 100L mo ia whakangao puranga, awhina i a koe ki te whakaheke i te CV% i waenga i nga waahanga rereke o nanoparticles koura 12. Penapena kia 80% nga rauemi tapanga (antigen, antibody, protein)
Ka aha i muri i te whakamahinga o te BALLYA Gold Nanoparticles?
Nanoparticles Koura Nui Te Kaha Rawa
1. Te rahinga whakaheke tae atu ki te 80% o nga taonga tapanga
Te arotake i te hanga 10,000 koura koura whakamātautau tere mo te kirika poaka African
Te nama take
Nanoparticles koura noa
BALLYA Koura Nanoparticle
Nga rauemi tapanga
5mg
2mg
Te whakamahinga o nga nanoparticle koura
1500ml (1 OD)
37.5ml (40 OD)
Te wa mahi
1 ra
4h
Te wa whakaputa
4 ra
3 ra
parau: i runga ake i te papa kua whakarerekehia kia rite ki te ahua whakaputa me te tikanga, hei tohu noa iho!
Te aromātai i te mahi whakangao koura koura whakamātautau tere mo te HIV
Rauemi Tapanga
Rōpū tapanga (ug / ml)
Tiaki
1 OD nanoparticles koura
40 OD nanoparticles koura
HIV Antigen 1
2.6
1.2
53.85 orau
HIV Antigen 2
1.6
1.2
25 orau
HBsAg Antibody
5.8
1.1
81.03 orau
parau: i runga ake i te papa kua whakarerekehia kia rite ki te ahua whakaputa me te tikanga, hei tohu noa iho!
Te aromātai i te mahi whakangao koura koura covid 19 whakamatautau tere
Rauemi Tapanga
Rōpū tapanga (ug / ml)
Tiaki
1OD nanoparticles koura
40OD nanoparticles koura
Antigen SARS-CoV-2
ASAR-27
2.8
1.3
53.57 orau
Antigen SARS-CoV-2
ASAR-28
3.2
1.5
53.13 orau
parau: i runga ake i te papa kua whakarerekehia kia rite ki te ahua whakaputa me te tikanga, hei tohu noa iho!
2. Penapenahia nga rauemi a te tangata me te waitohu 3. Whakapai ake i te hua 4. Whakamahia te centrifuge Papamahi kia pai ai te mahi papatipu 5. Whakaitihia te puranga CV%, kia mau ki nga hua kia rite 6. Poto te huringa whakawhanaketanga 50% pea
Nga momo nanoparticle koura BALLYA
Ngeru. Kare.
Rahi (nm)
Whakauru
mōkī
BGN00040
40
100OD
10ml - 1000ml
BGN00050
50
BGN00060
60
BGN00070
70
Ota BALLYA Koura Nanoparticles
Nga Paatai Auau me nga Whakautu
Me pehea te whakamahi i nga BALLYA Gold Nanoparticles?
1. Whakaritea te otinga buffer a PH tika rawa atu 2. Taapirihia te otinga buffer ki te 100 OD nanoparticles koura waimeha tae noa ki te 40 OD 3. Taapirihia he pūmua ki te 40 OD nanoparticles koura wai ki te tapanga
He aha te taunakitanga o te tapanga tapanga?
40 OD. I tenei kukume, ka awhina i te whakanui i te maaramatanga, te penapena i nga rauemi tapanga, te whakarei ake i te tae tae o te peeke.
He aha te rahinga tapanga pai rawa atu?
Ka whakawhirinaki atu ki to mahinga whakaputa me to momo hua, penei i te, hcg, hiv, covid 19 ... etc. He rereke nga mea katoa. Me mahi rangahau.
Me pehea te hanga i te otinga buffer me te PH e tika ana?
Tirohia koa nga paearu o te wairewa buffer.
Me pehea te rapu i te PH tino tika i roto i to punaha hangarau?
1. 100 OD kukume o koura koura, waimeha ki te 40 OD me te rongoa rereke rereke pH 2. Taapirihia he taua kounga antibody (e taunaki ana 50 ug / ml), tauhohe 2 haora 3. I te otinga o te urupare, tangohia he iti o te koura colloidal tapanga, ka rewa ki te 1 OD me te pH e taurite ana. 4. I roto i te 1 OD tapanga-koura, taapirihia te 10% NaCl kia 1% kukū, tirohia te tae i muri i te whakaranu, ko te tae te mea pai, ko te mea iti rawa iho, kaore ano hoki he kohinga whakahinuhinu, na, koira te pH tino pai
He aha te tauira buffer e tika ana ma BALLYA Gold Nanoparticles?
E 3 nga momo otinga buffer noa i raro nei:
Te whakataunga waipiro e paa ana ki te waikawa (0.01 mol / L)
pH
0.01M Waikawa Citric (ml)
0.01M Trisodium citrate dihydrate (ml)
3.0
18.6
1.4
3.6
14.9
5.1
4.0
13.1
6.9
4.6
10.3
9.7
5.0
8.2
11.8
5.6
5.5
14.5
6.0
3.8
16.2
6.6
1.4
18.6
Waikawa Citric: C6H8O7 · H2O: 0.01 mol / L; Wai: 2.101g / L Trisodium citrate dihydrate: Na3 C6H5O7 · 2H2O ： 0.01mol / L; Wai: 2.941 g / L
Te rongoa aukati e pa ana ki te Alkaline (0.02 mol / L)
pH
0.02M borax (ml)
0.02M waikawa boric (ml)
7.4
1.0
9.0
7.6
1.5
8.5
7.8
2.0
8.0
8.0
3.0
7.0
8.2
3.5
6.5
8.4
4.5
5.5
8.7
6.0
4.0
9.0
9.05
1.05
Borax: Na2B4O7 · 10H2O, 0.005M; Wai: 1.907 g / L Waikawa Boric: H2BO3, 0.02M; Wai: 1.237 g / L He maamaa te Borax ki te ngaro i te wai tioata, ana me waiho ki roto i te pounamu me te plug.
Rongoā Puka (Citric Acid + borax) (0.01 + 0.05)
pH
0.01M Waikawa Citric (ml)
0.05M borax (ml)
4.4
6
4
4.9
6.5
3.5
5.5
7
3
6.0
7.2
2.8
7.8
7.5
2.5
8.4
8
2
8.8
8.7
1.3
9.1
9.5
0.5
Borax: Na2B4O7 · 10H2O, 0.005M; Wai: 1.907 g / L Waikawa Citric: C6H8O7 · H2O: 0.01 mol / L; Wai: 2.101g / L He maamaa te Borax ki te ngaro i te wai tioata, ana me waiho ki roto i te pounamu me te plug.
Ko te tikanga tapanga e manakohia ana mo BALLYA Gold Nanoparticles Tikanga adsorption tinana:
Nga whakaritenga penei i raro: 1. Tohua te pH tino pai mo te pūmua tapanga 2. Tohua te otinga buffer e tika ana
3. Waimeha tika mai i te 100 OD ki te 40 OD 4. Taapirihia he pūmua ki te 40 OD nanoparticles koura te wai, te ohorere, te whakaoho ranei kia pai te tohatoha me te urupare 2hours 5. Taapirihia he otinga aukati, penei i te, BSA, OVA, me era atu Hohenga 20 min 6. Centrifuge, tango tipua, waimeha ki te pH tika
Ka taea e taatau te whakamahi i etahi atu tikanga tapanga mo nga BALLYA Gold Nanoparticles?
Ae, ko te tikanga. Ka taea hoki te whakamahi i te tikanga matū hei tapa.
Nga Kai o te BALLYA Gold Nanoparticles
E rua noa nga whakauru: 1. Nanoparticles koura 2. Wai Ma
He aha e kore ai e kitea PH i BALLYA Gold Nanoparticles?
He tino teitei te kukume, 100 OD. He noho taurite tonu, kaore he take mo te ururua i nga pakirehua kia kitea ai. He maama ki te whara i nga uiuinga pH.
He aha te PH o BALLYA Gold Nanoparticles?
He kūpapa tonu. pH = 7
He aha te take ka puta nga nanoparticle koura kua mate i aukati?
He paatai noa. Nga momo waitohu 'BSA kei a raatau ake tikanga ka whakaawe i te mahinga o nanoparticles koura, penei, te aukati i te hunga mate, te tupapaku i te mahinga whakamaroke, te whakaiti i te mohio, te whakapiki i te pai hape, me era atu I roto i tera ke, whakarereke i nga momo waitohu rereke o te aukati i te otinga, te whakamahi ranei i nga kaitautoko polymer kore-protein, penei i te, Polyethylene glycol , etc.
He aha te whakamahi i nga nanoparticle koura nui whakahirahira ka whakaiti i te whai hua o te tapanga?
Te mana'o nei au kei te anga atu koe ki te HOOK PAKANGA (te taumaha o nga paturopi, te antigen me nga pūmua).
Hei tauira, penei i nga wa o mua, me tapa te 1 OD me te 5ug / ml, na, i te wa e whakamahi ana koe i te 40 OD akene ki to whakaaro me whakamahi 5ug / ml x 40 = 200ug / ml. Engari ehara ko te pono. Neke atu i te mano nga wa whakamatautau, i kitea e maatau ko te 50ug / ml anake ka taea te whangai (40 OD) kia makona! Na, whakamatauhia te 50ug / ml, akene he miharo koe.
Nga Whakatupato
Rokiroki me te tuku: kaipuke pāmahana rūma, penapena i te 2- 8 ° C, kaua hei pouaka whakamātao Whakawhiti: i a koe e whakawhiti ana, e tohatoha ranei, whakamahia he pounamu ma hei karo i nga nanoparticle koura kua mate ora hounga: marama 12
