Tere Tere
Ko te Pipette Serological , e mōhiotia ana ko ngā pipette whakakorekore , e whakamahia ana hei ine tika i tētahi rahinga wai, ā, me whakamahi tahi me ētahi atu pipette tika.
He taputapu ine te pipette hei ine tika i tētahi rahinga wairewa. He pipette ine te pipette e whakamahia ana hei ine i te rahinga wairewa e tukuna ana. He ngongo karāhe angiangi me tētahi wāhanga nui kei waenganui. He koi te waha o te pito o raro, ā, he rārangi tohu kei te pito o runga o te kaki, he tohu tēnei mō te rahinga tika hei tango.
He taputapu taiwhanga kua whakatikatikaina te pipette serological , he mea tino pai mō ngā taiwhanga e hiahia ana ki te whakawhiti i te nui o te wai (1 mL ki te 50 mL). He maha ngā momo āhua o te pipette serological, tae atu ki te kirihou, te parakore, te whakamahi anō, me te karāhe, e whakarato ana i ngā kōwhiringa maha mō te taiwhanga.
Ko nga taonga hanga whanui e whakamahia ana ko te polystyrene, karaihe, me etahi atu. E 7 nga waahanga o te kaha, ara ko te 1.0ml, 2.0ml, 5.0ml, 10.0ml, 25.0ml, 50.0mL, 100ml. He rereke nga mowhiti tae ki te tohu i nga rereketanga rereke rereke, haunga ko te tinana ngongo he rereke nga tohu tauine tika, he pai ake mo te tautuhi me te whakamahi i te mahi. Ko etahi pito o nga putorino kua taapirihia ki nga pata taatari kia pai ai te aukati i te whakapoke-whiti i te wa e tuhi tauira ana koe.
Ka whakamahia whānuitia i roto i te ahurea kiko, te kitakitaiao, te haumanu, nga whakamatau rangahau putaiao me etahi atu waahanga e hiahia ana ki te whakahaere aseptic ki te whakawhiti wai. He pau te whakamahi i roto i te whare taiwhanga.
He mea hanga ēnei paipa ki te kirihou polystyrene kotahi te wahi, e whakakore ana i ngā hononga honohono e kitea ana i roto i ētahi atu paipa. Ka whakarewahia e ētahi kaihanga paipa ngā pito me ngā wāhanga waha o te paipa ki te tinana o te paipa. Ka mau te wai i ēnei "wāhi whakarewa" ā, ka puta pea he hapa. He mea hanga te paipa serological ki te kirihou polystyrene kotahi te wahi maeneene o te FDA, e whakamahi ana i tētahi hoahoa kore hononga hei whakakore i te tūponotanga o te wai toenga. Mā tēnei hoahoa ahurei ka tere ake te whakakī me te tuku, me te whakarite hoki i te rere katoa o te tauira me te whakarato i te tika teitei ake.
Ka whakamahia e te pipette serological he aukati aerosol hei ārai i te wai me te kohu wai kei poke i te pipette me te tauira. Kāore i rite ki ngā tampon, ka pai ake te tiakitanga o te tātari ārai aerosol i hangaia mai i ngā muka polyolefin kua herea, ā, ka whakaiti i te tūponotanga o te nui o te pipetting me te paru pea o ngā taputapu pipetting.
He poto ake ngā pipet serological i te nuinga o ngā momo pipet kē atu . Nā te hoahoa ipu poto me te kiato o ēnei pipet ka māmā ake te whakahaere me te neke haere puta noa i te taiwhanga. Ka kitea e koe he pai ake te mahi i ngā hāora roa i roto i ngā wāhi kuiti me ngā taupoki auahi taiwhanga me te pipet serological.
Ka taea e te pito i hangaia mō te tātaritanga te pupuri i te wai matotoru. Ko te diameter o roto o te pipette serological kei waenga i te 1.56 mm mō te 1 mL ki te 4.50 mm mō te 25 mL.
Kua tāia te pipette serological ki te tauine pango hei ārai i te koretake o te ine i te nui o te wai i mimitihia, i tukuna rānei. Mā tēnei mahi ka taea te whakarite i ngā pānui tika, ā, he iti iho i te 2% te hapa i te tauine katoa. Ka pā te tauine whakamuri ki ngā rōrahi e rima katoa, ā, kei roto i ngā pipette katoa te tauine kino (haunga te rōrahi 25 ml). Mō te tika, ko te tika o ngā pipette serological he rite ki te pikinga iti rawa (te nuinga o te wā 0.1 ml, te iti rawa 2 ml). Mena me nui ake te tika o tō rangahau, ka tūtohu mātou kia whakamahia e koe he pipette taiwhanga serological me te micropipette hei whakatutuki i ngā hua pai rawa atu.
Ko ngā paipa serological he mea whakakorekore noa iho, ā, kua oti te whakahoromata i mua. I te nuinga o te wā ka tākaihia ki roto i ngā kete nui i waenga i te 25 me te 50. Hei whakanui ake i te hua, kua hangaia ngā paipa serological katoa kia uru ki ngā kaiwhakahaere paipa hiko me ngā paipa. Mā tēnei kiato whānui ka taea e koe te mahi tika me te tere i ngā wā katoa.
E ai ki te nui me nga whakaritenga o te otinga whakawhiti, kowhiria he paipa me nga whakaritenga e tika ana. I te titration titration, ka whakamahia te pipette kia tika te paipa i te otinga me te whakahaere i te rahinga o te otinga whakamatautau.
Hipanga mo te whakamahi:
1. Kōwhiria he pipette o ngā whakaritenga e tika ana kia rite ki te rōrahi me ngā whakaritenga o te otinga ka whakawhitia. I roto i te tātari ira, ka whakamahia te pipette hei pipette tika i te otinga me te whakahaere i te nui o te otinga whakamātautau.
2. Tirohia te taputapu: tirohia mēnā kua pakaru te pūwero me te pito o te paipa . Mēnā kua pakaru, kāore e taea te whakamahi;
3. Horoia te taputapu: horoia ki te wai i te tuatahi, kātahi ka rumakina ki te wai horoi waikawa chromic, ko te tikanga whakahaere koia tēnei:
4. Mimia te wairewa: Rūrūtia te wairewa hei mimiti, ringihia he wāhanga iti o te wairewa hei mimiti ki roto i tētahi kapu iti ma, maroke hoki, mimitia te wai ki roto, ki waho hoki o te paipa kua horoia ki te pepa tātari, kātahi ka whakaurua ki roto i te kapu iti ka mimitia te wairewa. Kia tae ki te 1/3 o te rōrahi o te paipa , pēhia tonutia te waha o te paipa ki tō maihao matau, tangohia mai, pupurihia, ka hurihia te paipa kia rere te wairewa ki te pakitara o roto o te paipa, kātahi ka tangohia te wairewa mai i te pito o raro ki roto i te kapu wai para. Mā tēnei, ka taea te mimiti i te wairewa i muri i ngā horoinga e 3-4.
5. Whakauruhia te paipa kua horoia ki te wai hei mimiti ki roto i te 1~2 henimita i raro i te mata o te wai hei mimiti, ā, whakamahia te pōro taringa hei mimiti i te wairewa kia rite ki te tikanga whakahaere i runga ake nei. Ina piki te taumata wai i roto i te paipa ki te 1~2 henimita i runga ake i te rārangi tohu, āraia wawe te waha o te paipa ki tō maihao matau, hiki ake te paipa i waho i te mata o te wai, ka whakapā atu te paipa ki te pakitara o roto o te ipu mō tētahi wā, ka hiki ake ki runga ki te pepa tātari. Horoia te iti o te wairewa e piri ana ki raro o te paipa.
6. Whakatikatikahia te taumata wai: Tangohia tētahi atu kapu iti ma ki tō ringa maui, whakatakotoria te pito o te paipa ki te pakitara o roto o te kapu iti, kia piko te kapu iti, kia poutū te paipa, kia whakapae hoki te rārangi tohu ki te tirohanga.
Wetekina mārire tō matihao tohu kia rere mārire ai te wai i roto i te ngongo mai i te waha o raro. Ina tata te wai ki te tohu, pēhia te matihao tohu matau kia kaha, okioki mō tētahi wā, kātahi ka whai i te tikanga o runga hei whakanoho i te rārangi raro o te meniscus o te wai ki te taha o te tohu. Ina tata ki te tohu o runga, pēhia tonutia te waha o te ngongo ki tō matihao tohu. Whakatakotoria te pito ki te pakitara o roto o te ipu ka neke paku ki te waha o te ipu hei tango i ngā pata i te pito. Nekehia mārire te pipette ki te ipu e pupuri ana i te wai.
7. Tukua te wairewa: Whakatūria te pipette kia tika, whakatītahatia te kaiwhiwhi, kia tata te pito o raro o te ngongo ki te pakitara o roto o te kaiwhiwhi, tukua tō maihao tohu, tukua te wairewa kia rere ki raro i te pakitara o roto o te kaiwhiwhi, kia mutu te wairewa i roto i te ngongo, kia mau tonu ki te āhua tukunga mō te 15 hēkona, nekehia te pipette, te pipette rānei kia paku tawhiti ki te pakitara i te pito o te kaiwhiwhi hei tango i te pipette.
8. Horoia te taputapu: horoia te paipa ka whakatakoto ki runga i te whata paipa.
He mea hanga te pipette serological ki te rauemi polymer polystyrene mārama (GPPS) ā, e whakamahia whānuitia ana i roto i ngā whakamātautau rangahau haumanu me te pūtaiao pērā i te ahurea kiko me te bacteriology . Ka taea e ia te whakaiti i te piri o te wai ki te pakitara o te ngongo me te whakapai ake i te tika o te tauira. Ka tutuki i te hoahoa tauine rua-ara pakari ngā whakaritenga o te uta tauira me te whakaiti tauira, ngā tauine ngāwari ki te pānui, me ngā tauine kino nui-rahi, e watea ana mō te mimiti me te pānui wai. Kei ngā pito e rua he mono tātari miro hei ārai i te momi whakamuri me te waipuke.
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