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Malachite Green Rapid Test (1ppb)

Trimethoprim-Test-Kit

For meat products testing

Specs:25T / box
Testing Time:7-10 mins
Worldwide shipping, free sample
Introduction
The Malachite Green Rapid Test (1ppb) is based on the Colloidal Gold Immunochromatography to detect the MG in Aquaculture. This kit can be applied for on-site or lab rapid testing by various departments.

Performance Information
LOD:1ppb

Components
Test Kit
10 bags
Micro well
10 pcs
Extraction Agent 1 (purified water) 45ml
1 bottle
Extraction Agent 2 (Acetonitrile 45ml)
1 bottle
Extraction Agent 3 (powder)
10bottle
Extraction Agent 4 (1.5ml)
1 bottle
Extraction Agent 5 (3.5ml)
1 bottle
Product Instruction
1
Centrifuge Tube (15ml)
20pcs
Centrifuge Tube (5ml)
20pcs
Product Reference
 Oscillator
 Mixer
 Electronic Balance
 Centrifuge
 Termovap Sample Concentrator
 Pipette

Storage & shelf-life
 At 4~30℃, out of sun light & moisture, 12months

Preprocessing procedure
Refer to the instruction

Test procedure
1. Read the instruction, let the sample and test kit at room temperature(20-25℃).
2. Take out the test card, use it within 1h.
3. Place the card in flat, add 4-5 drop of sample (about 120µL) into the micro well to dissolve the reagent completely. Then, incubate for 4mins at room temperature.
4. Pipette all dissolved sample into the sample well.
5. Read the result within 5-8mins. Other results are invalid.
Interpretation of results
Negative: line T is same or stronger than C
Positive: line T lighter than C
Invalid: line C does not appear 

Results Illustration
Negative:
Positive:
Invalid:
Precautions
Refer to the instruction

If you want to know more, please contact us

Trivia questions : What is Malachite green?

What is malachite green?

Malachite green meaning

Although it is called malachite green, in fact, it does not contain malachite, only the two are similar in color. Malachite and malachite green are two completely different substances.
Malachite green is a toxic triphenylmethane chemical. It is both a dye and a fungicidal, bactericidal, and parasiticidal drug. Long-term excessive use can cause cancer, and it is prohibited to be added in the aquaculture sector.

Malachite green structure

Malachite green is a synthetic organic compound. It is produced by mixing 1 mole of benzaldehyde (Benzaldehyde) and 2 moles of xylylamine in concentrated hydrochloric acid and heating to condense into Leuco base. Under acidic conditions, lead peroxide is added to oxidize. Pigment base is precipitated in the alkaline solution. It belongs to the triphenylmethane type green dye.

Malachite green synthesis

Malachite green is prepared by the condensation of benzaldehyde and dimethylaniline to give colorless malachite green.

Malachite green uses

Malachite green dye

Malachite green can be used as a dye for silk, leather and paper. Commercial malachite green dye is a pigment that is dissolved in hot oxalic acid solution and cooled to obtain the crystal of oxalate, 2C23H24N2. C2H2O4. Or after neutralizing with hydrochloric acid, a certain amount of zinc chloride ZnCl2 is added to crystallize the zinc chloride double salt 3C23H25N2CL.2ZnCl2 to become a green alkaline dye. It is used to dye wool, silk, leather, etc. It is a full-time dye.
Malachite green can be used as a biological staining agent to stain cells or cell tissues to blue-green for convenient research under a microscope. It can be used to stain host cells of plant virus infection, staining of bacteria, spores, and red blood cells and roundworm eggs.

Malachite green stain

Malachite green can be used as a dye for silk, leather and paper. Commercial malachite green dye is a pigment that is dissolved in a hot oxalic acid solution and cooled to obtain oxalate 2C23H24N2 crystals. C2H2O4. Or after neutralizing with hydrochloric acid, a certain amount of zinc chloride ZnCl2 is added to crystallize the zinc chloride double salt 3C23H25N2CL.2ZnCl2 to become a green basic dye. Used for dyeing wool, silk, leather, etc. It is a full-time dye.
Malachite green staining biological stain is used to stain cells or cell tissues to blue-green for easy research under a microscope. It can be used to stain plant virus-infected host cells, bacteria, spores, red blood cells, and worm eggs.

Malachite green fungicide

Malachite green can be used to treat parasites, fungi, or bacterial infections of fish or fish eggs. It is particularly effective against the fungus Saprolegnia. Fish eggs on fish farms can be infected with this fungus. Malachite green is also commonly used to treat freshwater aquatic products affected by parasites. It is used as a bacteriostatic agent or amoeba protozoal agent. It has high toxicity, high residue and other side effects on marine animals such as fatty carp and catfish. Therefore, it is usually only half the amount when used.

Other aspects

Used as a bacterial polysaccharide reagent;
Used as a clinical diagnostic reagent (measurement of inorganic phosphate);
Used for photometric determination of gallium, tantalum and antimony; catalytic photometric determination of tungsten; spot test for sulfite and cerium and tungsten;
Used as acid-base indicator, pH 0.0 (yellow) ~ 2.0 (green), 11.6 (blue-green) ~ 14 (colorless);
Used as redox indicator

Malachite green for fish

Malachite green has special effects on water mold and fish eggs. There are no special drugs on the market that can solve water mold in a short time. This is why this product is banned in the aquaculture industry. It has been banned for many years, and the fundamental reason for aquaculture farmers to continue to use malachite green in violation of regulations. In other respects, it can also be used for the treatment of gill mold, melons, trichophyton, ringworm, oblique tuberculosis, third-generation worm disease, and other bacterial diseases. China's Ministry of Agriculture has listed malachite green as a banned aquatic product, and ornamental fish that are not food fish can also be used.

Malachite green hazard

Studies have found that when malachite green enters the body of aquatic animals, it is rapidly metabolized into a fat-soluble colorless malachite green. Malachite green has potential carcinogenic, teratogenic, and mutagenic effects. Its use in the breeding industry has not been approved by the US Food and Drug Administration (FDA); according to EU Act 2002/675 / EC, animal sources The total amount of malachite green and colorless malachite green residues in sex food is limited to 2 μg / kg; Japan's positive list also clearly stipulates that malachite green residues cannot be detected in imported aquatic products; China's agricultural industry standard "NY5071- 2002 Malachite green is also listed as a banned drug in the Guidelines for the use of pollution-free food fish medicine.

Malachite green assay

The detection methods of malachite green are mainly physical and chemical detection methods and immunological detection methods. The physical and chemical detection methods include: thin layer chromatography, spectrophotometry, high pressure liquid chromatography, liquid chromatography mass spectrometry, gas chromatography mass spectrometry, etc., compared with immunological detection methods are more commonly used.

Summary

Since there are no cheap and effective alternatives, the use of malachite green in aquaculture has been repeatedly banned. Malachite green is potentially carcinogenic, teratogenic, and mutagenic, so it poses a huge health threat to consumers. It is timely discovered that malachite green in aquatic products has become what every consumer wants to do. thing.
The Malachite Green Rapid Test (1ppb) kit provided by BALLYA can effectively detect malachite green contained in aquatic products, which can further protect the safety of consumers. This kit is easy to operate, and has a short test time and high accuracy. 
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