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immunochromatography

Yuav ua li cas txhawm rau txhim kho qhov ntsuas nrawm raws li immuno-chromatography?

Muab lo rau  Cuaj hlis 11, 2020, Kho los ntawm Jason, Qeb  

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Immunochromatography yog dab tsi?
Lub hauv paus ntsiab lus ntawm immunochromatography
Txoj kev ntsuas feem ntau hauv immunochromatography
Cov xim feem ntau siv hauv immunochromatography
Dab tsi yog colloidal kub?
3 hom kev ntsuas ntsuas raws li colloidal kub immunochromatography
Cov ntaub ntawv raw siv hauv kev txhim kho immunochromatography
Cov cuab yeej siv hauv kev txhim kho immunochromatography
Cov txheej txheem tsim khoom hauv kev txhim kho immunochromatography
Yuav siv colloidal kub immunochromatography rau kev kuaj xyuas zaub mov li cas?
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Immunochromatography yog dab tsi?

Immunochromatography (immu-nochromatography assay, ICA) yog hom tshiab ntawm kev siv tshuaj tiv thaiv kab mob uas tau tshwm sim nyob rau xyoo 1980s. Nws yog qhov tshuaj tiv thaiv kab mob yooj yim thiab nrawm tsim los ntawm kev tiv thaiv kev tiv thaiv kab mob (IFA).

Lub hauv paus ntsiab lus ntawm immunochromatography

Lub hauv paus ntsiab lus ntawm immunochromatography yog kho cov tshuaj tiv thaiv tshwj xeeb ntawm ib cheeb tsam ntawm cov nitrocellulose daim nyias nyias. Thaum cov nitrocellulose qhuav kawg yog raus rau hauv cov qauv (analyte), vim capillary kev txiav txim, tus qauv yuav txav mus rau pem hauv ntej ntawm daim nyias nyias, thaum tsiv mus rau thaj chaw uas cov tshuaj tiv thaiv raug kho, cov antigen sib raug hauv cov qauv tshwj xeeb khi rau cov tshuaj tiv thaiv. . Yog tias stained nrog immunogold lossis immunoenzyme, thaj chaw tuaj yeem pom cov xim xim kom ua tiav cov tshuaj tiv thaiv kab mob tshwj xeeb.

Txoj kev ntsuas feem ntau hauv immunochromatography

Radioimmunoassay (RIA)

RIA thev naus laus zis siv cov tshuaj antigens lossis cov tshuaj tiv thaiv uas sau nrog isotopes hluav taws xob (xws li 125I, zhi32P, 3H, thiab lwm yam) txhawm rau ntsuas cov tshuaj tiv thaiv hluav taws xob ntawm γ-rays lossis β-rays nrog lub aodao-ray ntes lossis kua scintillation txee los txiav txim siab cov tshuaj tiv thaiv lossis antigen ntim tshuab. Nws suav nrog radioimmunoassay tus yam ntxwv los ntawm daim ntawv lo antigen thiab kev tshuaj tiv thaiv kab mob immunoradiometric (IRMA) yam ntxwv los ntawm daim ntawv lo tshuaj tiv thaiv kab mob. Yav dhau los feem ntau siv cov txheej txheem kev sib tw ua kua, uas ntsuas ob qho macromolecular antigens thiab antigens me me molecule; tom kawg siv txheej txheem txheej txheem txhawm rau ntsuas macromolecular antigens.

RIA koom nrog feem ntau hauv cov txheej txheem tsim tshuaj tua kab ua ntej, thiab tsim cov hluav taws xob ntawm dieldrin, aldrin, 2,4-D thiab 2,4,5-T, parathion thiab paraquat. Immunoassay. Txawm hais tias txoj hauv kev no nkag siab heev (RIA feem ntau yog 10-9g, 10-12g, lossis txawm tias 10-15g) thiab muaj ntau yam kev siv, cov txee kim yuav tsum tau ua RIA, thiab tseem muaj teeb meem xws li hluav taws xob thiab pa phem . Daim ntawv thov thiab kev txhim kho ntawm thaj chaw tshawb pom raug rau qee qhov kev txwv, thiab maj mam hloov los ntawm lwm txoj hauv kev tiv thaiv kab mob.

Enzyme immunoassay (enzymeimmunoassay, EIA)

EIA yog lub tshuab thev naus laus zis tsim tom qab RIA. Lub hauv paus ntsiab lus tshawb pom zoo ib yam li cov xov tooj cua radioimmunoassay, tab sis daim ntawv lo uas siv yog ib qho enzyme, uas yog cov koom ua ke nrog cov tshuaj tiv thaiv kab mob tshwj xeeb ntawm antigens thiab cov tshuaj tiv thaiv nrog kev ua haujlwm siab catalysis ntawm enzymes. Cov dej num ntawm cov enzyme khi rau theem ruaj khov tau ntsuas los txiav txim siab ntsuas qhov ntsuas ntawm cov khoom. Cov enzymes siv ua cov cim suav nrog horseradish peroxidase (HRP) thiab alkaline phosphatase (AKP), glucose oxidase (GO), urease (urease) thiab ntxiv rau.

Kev txhawb nqa ruaj rau cov tshuaj tiv thaiv daim ntawv lo suav nrog suav nrog cov yas yas polystyrene thiab daim nyias nyias. Tam sim no, feem ntau ntawm 96-zoo microtiter daim hlau (MTP) tau siv los ua kev txhawb nqa. Hom phaj no muaj peev xwm tshawb pom loj thiab muaj ntau tus qauv, thiab tsuas yog tus nyeem microplate yooj yim thiaj tuaj yeem nrhiav cov ntaub ntawv raug. Qee tus kws tshawb fawb kuj tseem siv cov hlaws sib nqus ua cov khoom siv rau qib EIA tshawb fawb. Lub hauv paus ntsiab lus yog qhwv cov ntaub ntawv polymer (polystyrene, polyvinyl chloride, thiab lwm yam) nyob sab nraud ntawm cov hlau me me (Fe2O3, Fe3O4), thiab tom qab ntawd sib txuas nrog cov amino pawg. (-NH2), carboxyl (-COOH), hydroxyl (-OH) thiab lwm pab pawg ua haujlwm tau ua ke nrog cov tshuaj tiv thaiv kab mob lossis antigens los ua cov tshuaj tiv thaiv kab mob. Qhov zoo ntawm txoj hauv kev no yog tias microbeads muaj thaj tsam loj tshwj xeeb, muaj peev xwm nqus tau zoo, thiab tuaj yeem raug ncua hauv cov kua theem kom nrawm thiab zoo ib yam txhawm rau ntes cov tshuaj ntsuam hauv cov qauv. Tom qab siv daim teb sib nqus, cov microbeads tuaj yeem sib cais los ntawm cov qauv daws sai sai, yog li txo kev nrhiav pom. Sijhawm, txhim kho kev paub qhov tseeb.

Vim tias enzyme-labeled reagents tau yooj yim los npaj, ruaj khov, thiab pheej yig, thiab qhov kev nkag siab ntawm enzyme immunoassay nyob ze rau ntawm cov thev naus laus zis thev naus laus zis, EIA thev naus laus zis tau tsim nrawm nyob rau xyoo tas los no, thiab ntau txoj hauv kev EIA tau tsim. Ntawm lawv, enzyme-txuas immunosorbent assay (enzyminkinked immunosorbent assay, ELISA) yog tam sim no siv dav tshaj plaws enzyme immunoassay txheej txheem hauv tshuaj tua kab tshuaj tua kab.

Fluorescence immunoassay (fluorescence immunoassay, FIA)

Lub hauv paus ntsiab lus tseem ceeb ntawm FIA kuaj pom yog txhawm rau txhawm rau txhawm rau ua kom muaj qhov tshwj xeeb tshwj xeeb ntawm antigens thiab cov tshuaj tiv thaiv nrog qhov ntsuas tau yooj yim ntawm cov tshuaj fluorescence, thiab siv cov tshuaj fluorescent ua cov neeg taug kev los cim cov tshuaj tiv thaiv, antigens lossis hapten molecules los npaj cov tshuaj fluorescent tshwj xeeb zoo. Thaum cov tshuaj fluorescent hauv cov tshuaj tiv thaiv kab mob antigen-antibody tau tawg los ntawm ultraviolet lub teeb lossis lub teeb xiav, nws tuaj yeem nqus tau lub zog hluav taws xob thiab nkag mus rau hauv lub xeev zoo siab. Thaum nws rov qab los rau hauv lub xeev hauv av los ntawm lub xeev zoo siab, nws tuaj yeem tso tawm lub zog nqus lub zog nyob rau hauv daim ntawv ntawm hluav taws xob hluav taws xob, ua rau muaj fluorescence. Plotting concentration tshuaj tua kab-fluorescence siv txoj kab nkhaus tuaj yeem tsim nyog thiab muaj nuj nqis pom cov tshuaj tua kab nyob hauv cov qauv.

Fluorescein haum rau daim ntawv lo ntawm cov tshuaj tiv thaiv, antigens lossis hapten molecules yuav tsum ua tau raws li qhov yuav tsum tau ua:

  1. Nws yuav tsum muaj cov pab pawg tshuaj lom neeg uas tuaj yeem tsim cov ntawv cog lus ruaj khov nrog cov protein molecule, lossis hloov pauv tau yooj yim rau hauv cov tshuaj tiv thaiv yam no yam tsis ua kom puas nws cov qauv fluorescent;
  2. Tom qab daim ntawv lo, cov qauv tshuaj thiab cov khoom ntawm fluorescein thiab cov tshuaj tiv thaiv kab mob lossis tshuaj tiv thaiv kab mob yuav tsis hloov pauv;
  3. Qhov ua tau zoo fluorescence yog siab, thiab qhov yuav tsum tau ua kom ruaj nrog cov protein yog me me;
  4. Cov txheej txheem ntawm kev khi cov fluorescein rau cov protein yog yooj yim thiab nrawm, thiab tsis muaj fluorescein dawb thiab nws cov khoom lag luam raug rhuav tshem tau yooj yim;
  5. Kev sib txuas muaj kev ua haujlwm ruaj khov nyob rau hauv cov xwm txheej dav dav thiab tuaj yeem khaws cia ntev.

Chemiluminescence immunoassay (luminescentimmunoassay, LCIA)

LCIA tuaj yeem faib ua chemiluminescent immunoassay (chemiluminescent immunoassay, CLCIA) thiab bio-luminescent immunoassay (bio-luminescent immunoassay, BLCIA).

Xyoo 1976, Shroeder thawj zaug tsim cov tshuaj sib xyaw ua ke uas siv cov tshuaj biotin (B) -avidin (A). Tom qab ntawd, Halman thiab Velan txuas nws mus rau cov kab ke sib txawv, uas tam sim no tau nkag mus rau hauv kev tshawb fawb lom. Ntau yam teb. Lub hauv paus yog los qhia qhov sib xyaw ua ke ntawm antigen thiab antibody los ntawm luminescence. Thaum lub luminescent daim ntawv lo ua ke nrog cov tshuaj tiv thaiv kab mob los yog antigen, cov txheej txheem cuam tshuam nrog cov enzyme, lossis redox cov tshuaj tiv thaiv nrog tus neeg sawv cev luminescent, lossis cov tshuaj fluorescent (xws li rubrene, thiab lwm yam) Zoo siab thiab tso lub zog tawm. Thaum kawg, qhov pom kev ci ci tau ntsuas nrog lub ntsuas hluav taws xob rau kev tshuaj xyuas ntau. Feem ntau siv cov cim luminescent yog horseradish peroxidase (HRP), luminol (luminol), isoluminol (isoluminol), lophine (lophine), lucigen (lucigen), ob npaug (2, 4, 6) -Trichlorobenzene) oxalate, biphenyltriol thiab 6 [N- (4-diaminobutyl) -N-ethyl] -amino-2,3-dihydrophenazine-1,4-dione (ABEI) thiab lwm yam. (lossis tshuaj tiv thaiv kab mob) hauv qee qhov kev daws teeb meem pH tsis zoo, nws yuav tso tawm lub teeb nyob hauv qhov kev nqis tes ua ntawm kev koom tes (xws li H2O2, thiab lwm yam), thiab nws qhov kev siv teeb pom kev zoo yuav zoo ib yam li cov khoom ntsuas. Nws yog qhov sib npaug rau qhov kev mloog zoo, yog li nws tuaj yeem siv rau kev tshuaj xyuas ntau.

Luminescence immunoassay muaj qhov zoo ntawm qhov tshwj xeeb muaj zog, nkag siab tau zoo (kuaj pom tsis pub dhau 10-15mol/L), nrawm (1 ~ 3h), thiab muaj cov khoom siv luminescent yooj yim. Txawm li cas los xij, nws cov txheej txheem luminescence thiab kev siv feem ntau cuam tshuam los ntawm cov txheej txheem tshuaj lom neeg ntawm cov tshuaj luminescent nws tus kheej, pH ntawm nruab nrab, cov khoom sib xyaw luminescent thiab cov hlau tsis huv.

Kev ntsuam xyuas kub immunochromatography (goldimmuno-chromatography assay, GICA)

Lub hauv paus ntsiab lus ntawm GICA kev kuaj pom yog txhawm rau tiv thaiv cov ligands (cov tshuaj tiv thaiv lossis antigens) ntawm daim nyias nyias microporous xws li nitrocellulose daim nyias nyias hauv cov kab. Daim ntawv lo lo lo lo lo lo lo lo ntxhuav kub tseem raug kho nrog ligands lossis lwm yam tshuaj hauv lub xeev qhuav ntawm cov khoom nqus tau. Capillary ua rau cov qauv piv txwv ua luam dej ntawm cov kab chromatographic. Thaum nws ua luam dej mus rau qhov cim xim kub colloidal, yog tias tus qauv muaj tus neeg txais khoom los kuaj, thawj kauj ruam yog qhov tshwj xeeb kev tiv thaiv kab mob los tsim kev tiv thaiv kab mob Thaum cov tshuaj txuas ntxiv ua luam dej mus rau thaj tsam txheej txheej, theem ob ntawm kev tiv thaiv kab mob tshwj xeeb tshwm sim, thiab cov txheej txheem tiv thaiv kab mob tau raug daig hauv thaj chaw txheej txheej, thiab cov qhab liab tau qhia los ntawm daim ntawv lo lo lo lo lo lo lo lo ntsiab muag kub (nrhiav kom pom Band), thaum daim ntawv lo dawb hla hla qhov ntsuas pom thiab tau txiav tawm ntawm daim ntawv lo . Kev txiav txim siab kom muaj txiaj ntsig lossis muaj txiaj ntsig tuaj yeem ua tiav los ntawm kev kuaj pom tias muaj lossis tsis muaj xim lossis ntxoov ntxoo ntawm cov xim ntawm txoj siv 2.

Colloidal Kub Xeem Cov Khoom Ntsuas

GICA txoj hauv kev muaj qhov zoo ntawm kev yoo mov (5-20min), pheej yig, muaj txiaj ntsig zoo, tsis muaj kev txawj ua haujlwm nyuaj thiab cov cuab yeej tshwj xeeb, thiab nqa tau yooj yim. Txawm li cas los xij, piv nrog lwm txoj hauv kev tshuaj tiv thaiv kab mob, qhov ntsuas pom qhov ua tau zoo ntawm cov txheej txheem no qis dua me ntsis, thiab nws yog qhov tsim nyog rau kev txiav txim kom muaj txiaj ntsig zoo ib nrab lossis ib nrab. Tam sim no, txoj kev no tau siv hauv ntau qhov kev tshawb fawb xws li tshuaj thiab biology, thiab tau siv dav tshwj xeeb hauv cov tebchaws tau tsim.

Kev sib txuas ua ke thev naus laus zis ntawm immunoassay thiab ntsuas kev ntsuas tshuab

Kev siv ib qho txheej txheem IA los tshuaj xyuas cov tshuaj tua kab muaj tsawg dua cov ntaub ntawv, thiab kev xaiv cov txheej txheem tshuaj thiab tshuaj lom neeg tsis zoo. Kramer thiab al. siv immunoassays thiab kua chromatography (LC) ua ke, yog li ua kom yooj yim txoj kev ntsuas thiab txhim kho kev txheeb xyuas qhov ua tau zoo. Kev sib xyaw ua ke ntawm LC-IA suav nrog kev sib cais muaj peev xwm ntawm LC nrog qhov ua tau zoo thiab qhov tshwj xeeb ntawm IA. Txoj kev ntsuas no tshwj xeeb tshaj yog tsim rau kev tshuaj xyuas ntau qhov sib xyaw thiab kev tshuaj xyuas microanalysis. Kev sib xyaw ua ke ntawm cov tshuaj tiv thaiv kab mob thiab roj chromatography lossis huab hwm coj loj (GC lossis MS) tuaj yeem txo qis kev cuam tshuam hauv kev tshuaj xyuas cov tshuaj tua kab lossis cov tshuaj metabolites nrog cov qauv zoo sib xws los txo qhov tsis zoo.

Cov xim feem ntau siv hauv immunochromatography

Colloidal kub nanoparticle
Latex particle
Cov pa roj carbon particle
Sib nqus hlaws
Fluorescent dyes
Quantum dots
Upconversion luminescent

Dab tsi yog colloidal kub?

colloidal-kub-particle
colloidal-kub-particle

Colloidal kub, tseem hu ua kub nanoparticles, yog qhov ruaj khov, zoo ib yam, thiab ib leeg-tawg tawg raug tshem tawm ntawm cov khoom kub tau raug tshem tawm hauv cov kua tom qab cov ntsev ntsev raug txo kom kub. Cov khoom kub tau tsim los ntawm cov kub atom thiab ob txheej ion txheej puag ncig nws.

Cov xim ntawm kev daws teeb meem yog nyob ntawm cov xim ntawm cov khoom tawg, tawg ntawm cov khoom tawg tawg, thiab hom teeb pom kev zoo, txawm tias nws tau tawg lossis teeb pom kev. Qhov me me thiab qhov siab dua qhov kev tawg, qhov luv luv ntawm lub teeb tawg. Rau hydrosol ntawm tib yam khoom, qhov loj me me sib txawv, thiab xim kuj txawv.

Piv txwv li, cov xim sib xyaw ua kom muaj xim sib txawv ntawm 5-20 nm, nqus dej yoj ntawm 520 nm, yog xim liab xim; qhov loj ntawm 20-40 nm feem ntau nqus lub teeb ntsuab nrog lub nthwv dej ntawm 530 nm, thiab cov tshuaj yog xim liab tsaus; 60 nm colloidal kub particle feem ntau nqus cov nthwv dej 600 nm txiv kab ntxwv lub teeb, cov tshuaj yog xiav-violet. Cov xim kub colloidal feem ntau siv hauv immunohistochemistry yog nyob ntawm thaj tsam ntawm 5-60 nm, thiab cov tshuaj zoo li liab.

3 hom kev ntsuas ntsuas raws li colloidal kub immunochromatography

Cov xim uas nrov tshaj plaws yog kub nanoparticle. Colloidal kub thev naus laus zis uas suav nrog cov tshuaj tiv thaiv kab mob antigen-antibody teb thiab colloidal gold labeling thev naus laus zis rau qhov muaj txiaj ntsig thiab muaj nuj nqis pom ntawm antigen thiab cov tshuaj tiv thaiv cov ntsiab lus. Vim nws qhov zoo xws li nrawm nrawm, yooj yim, tus nqi qis thiab muaj kev nyab xeeb zoo, kev ntsuas kub colloidal tau siv dav hauv kev tshuaj xyuas kab mob.

Tam sim no, feem ntau siv txoj hauv kev kuaj pom colloidal kub nrhiav lub platform suav nrog ob-tshuaj tiv thaiv kab mob Sandwich method, txoj kev sib tw thiab txoj hauv kev tsis ncaj. Muaj qhov sib txawv loj ntawm txoj kev ntsuas thiab ntsuas.

Ob-Antibody Sandwich Method

Txoj kev siv tshuaj tiv thaiv kab mob thib ob yog qhov siv ntau tshaj plaws txhawm rau txhawm rau txheeb xyuas cov xim kub colloidal, uas yog siv los txhawm rau txheeb xyuas cov biomolecules loj thiab cov antigens me me. Txoj hauv kev ob-tshuaj tiv thaiv kab mob nyuaj nyuaj yuav tsum tau npaj cov tshuaj tiv thaiv ua ke ntawm cov tshuaj antigen los kuaj. Ib qho tshuaj tiv thaiv kab mob tau sau nrog colloidal kub thiab tsau rau ntawm lub ncoo sib txuas, thiab lwm qhov tshuaj tiv thaiv tau tsau ntawm kab kab (T kab) ntawm NC daim nyias nyias. Ib qho ntxiv, nws yog qhov yuav tsum tau npaj cov tshuaj tiv thaiv kab mob thib ob uas tshwj xeeb khi rau daim ntawv lo-kub cov tshuaj tiv thaiv kab mob (piv txwv li, colloidal kub-daim ntawv lo tshuaj tiv thaiv kab mob) thiab kho nws ntawm kab kab (C kab) ntawm NC daim nyias nyias.

sab-ntws-immunoassay
sab-ntws-immunoassay
ib sab-ntws-sawb

Thaum cov qauv tau ntxiv rau hauv kab txaij, nws yuav txav mus rau cov qauv ntsuas phoo, sib txuas ncoo, T kab, C kab thiab nqus dej ncoo (wicking ncoo) ua ntu zus los ntawm txoj cai capillary. Cov tshuaj tiv thaiv ntawm txhua theem tau qhia hauv cov lus hauv qab no:

 Qauv txav T conjugate ncooQauv txav mus rau T kabQauv txav mus rau kab Ctshwm sim
Anaylte muaj antigenKub-sau cov tshuaj tiv thaiv kab mob tau txais antigen, dhau los ua qhov nyuajCov tshuaj tiv thaiv kab mob ntawm kab T yuav ntes qhov nyuaj thiab cov xim xim yuav pomQhov tshuaj tiv thaiv kab mob thib ob yuav ntes cov tshuaj tiv thaiv kub-cim npe thiab xim xim pomOb kab pom
Anaylte tsis muaj antigenTsis muaj cov tshuaj tiv thaivTsis muaj xim hloovTib yam li saum toj noTsuas pom kab C nkaus xwb

Yog li ntawd, thaum ob kab xim liab tshwm rau ntawm kab txaij, nws txhais tau tias cov khoom yuav raug sim hauv cov qauv yog qhov zoo; thaum tsuas muaj kab C ntawm kab txaij, nws txhais tau tias cov khoom yuav raug sim hauv cov qauv tsis zoo. Qhov siab ntawm cov tshuaj yuav muaj cov xim muaj zog ntawm T-kab.

Txoj Kev Sib Tw

Cov tshuaj antigens me me nyuaj rau npaj cov tshuaj tiv thaiv ua ke (lub cev hnyav hnyav me me, nws nyuaj rau nrhiav ob qhov chaw khi rau ob qhov tshuaj tiv thaiv los khi tib lub sijhawm), yog li cov tshuaj tiv thaiv me me tsis tuaj yeem kuaj pom los ntawm ob txoj kev tiv thaiv kab mob nyuaj nyuaj. Rau cov tshuaj antigens me me, feem ntau yog siv txoj hauv kev sib tw rau kev ntsuas.

Hauv txoj kev sib tw, daim ntawv lo-tiv thaiv kab mob kub tau tsau rau ntawm cov cojugate pad o, thiab T kab ntawm NC daim nyias nyias tau tsau nrog macromolecular txuas ua ke antigen los kuaj (cov tshuaj tiv thaiv me me tsis tuaj yeem ncaj qha rau ntawm NC membrane.) , nws yog qhov yuav tsum tau siv tshuaj ob peb lub molecules me me rau BSA thiab lwm yam tshuaj macromolecular thiab tom qab ntawd ua rau lawv tsis khov rau ntawm NC daim nyias nyias). Qhov tshuaj tiv thaiv kab mob thib ob uas tuaj yeem tshwj xeeb khi rau daim ntawv lo-kub cov tshuaj tiv thaiv tsis tau txav mus los ntawm kab C.

lateral-flow-assay-kev loj hlob
lateral-flow-assay-kev loj hlob
ib sab-ntws-ntaus ntawv
ib sab-ntws-ntaus ntawv

Thaum cov qauv tau ntxiv rau hauv kab txaij, nws yuav txav mus rau cov qauv ntsuas phoo, sib txuas ncoo, T kab, C kab thiab nqus dej ncoo (wicking ncoo) ua ntu zus los ntawm txoj cai capillary. Cov tshuaj tiv thaiv ntawm txhua theem tau qhia hauv cov lus hauv qab no:

 Qauv txav T conjugate ncooQauv txav mus rau T kabQauv txav mus rau kab Ctshwm sim
Anaylte muaj antigenKub-sau cov tshuaj tiv thaiv kab mob tau txais antigen, dhau los ua qhov nyuajTsis muaj antigen tuaj yeem ntes tau. Tsis muaj xim bandQhov tshuaj tiv thaiv kab mob thib ob yuav ntes cov tshuaj tiv thaiv kub-cim npe thiab xim xim pomTsuas pom kab C nkaus xwb
Anaylte tsis muaj antigenTsis muaj cov tshuaj tiv thaivNqa daim ntawv lo-kub daim ntawv tshuaj tiv thaiv kab mob thiab xim xim pomTib yam li saum toj noOb kab pom

Yog li ntawd, thaum ob kab xim liab tshwm rau ntawm kab txaij, nws txhais tau tias cov khoom yuav raug sim hauv cov qauv tsis zoo; thaum tsuas muaj kab C ntawm kab txaij, nws txhais tau tias cov khoom yuav raug sim hauv cov qauv yog qhov zoo. Qhov siab ntawm cov tshuaj yuav muaj cov xim muaj zog ntawm T-kab.

Indirect Kev

Txoj hauv kev tsis ncaj ncees feem ntau yog siv los tshuaj xyuas cov tshuaj tiv thaiv kab mob. Yog tias txoj hauv kev tsis sib xws, lub ncoo sib txuas ua ke nrog cov protein A sau nrog kub colloidal (Cov protein A tuaj yeem khi rau cov tshuaj tiv thaiv tshwj xeeb). Txoj kab T ntawm NC daim nyias nyias tau tsau nrog antigen uas tuaj yeem tshwj xeeb khi rau cov tshuaj tiv thaiv kab mob los sim, thiab C kab ntawm NC daim nyias nyias tau tsau nrog cov tshuaj tiv thaiv Protein A.

immunochromatographic-xeem
immunochromatographic-xeem
ib sab-ntws-sawb

Thaum cov qauv tau ntxiv rau hauv kab txaij, nws yuav txav mus rau cov qauv ntsuas phoo, sib txuas ncoo, T kab, C kab thiab nqus dej ncoo (wicking ncoo) ua ntu zus los ntawm txoj cai capillary. Cov tshuaj tiv thaiv ntawm txhua theem tau qhia hauv cov lus hauv qab no:

 Qauv txav T conjugate ncooQauv txav mus rau T kabQauv txav mus rau kab Ctshwm sim
Anaylte muaj cov tshuaj tiv thaiv kab mobKub-sau lo lus Protein A ntes cov tshuaj tiv thaiv kab mob, dhau los ua qhov nyuajAntigen ntes cov tshuaj tiv thaiv, cov xim xim pomProtein A ntes los ntawm cov tshuaj tiv thaiv proten A antibody thiab xim band pomOb kab pom
Anaylte tsis muaj tshuaj tiv thaivTsis muaj cov tshuaj tiv thaivAntigen tsis tuaj yeem ntes antigen, xim tsis hloovCov ntawv lo-kub cim cov tshuaj tiv thaiv kab mob thib ob, cov xim xim pomTsuas pom kab C nkaus xwb

Yog li ntawd, thaum ob kab xim liab tshwm rau ntawm kab txaij, nws txhais tau tias cov khoom yuav raug sim hauv cov qauv yog qhov zoo; thaum tsuas muaj kab C ntawm kab txaij, nws txhais tau tias cov khoom yuav raug sim hauv cov qauv tsis zoo. Qhov siab ntawm cov tshuaj yuav muaj cov xim muaj zog ntawm T-kab.

Cov ntaub ntawv raw siv hauv kev txhim kho immunochromatography

  1. Antigen thiab antibody
  2. Bovine Serum Albumin Cov
  3. Thaub qab daim npav ib sab ntws
  4. Nitrocellulose daim nyias nyias
  5. Qauv ncoo (Wicking ncoo)
  6. Conjugate ncoo
  7. Nqus plua plav
  8. Ntshav sib cais daim nyias nyias (xaiv)
  9. Cassette rooj plaub (kev xaiv)

Cov cuab yeej siv hauv kev txhim kho immunochromatography

  1. Robotic dispensing system (Dispensor)
  2. Sawb cutter rau lateral flow assay strip
  3. Micropipette
  4. Lub tshuab nqus tsev khov
  5. Lub tshuab nqus tsev cub
  6. Kev kub ceev tub yees centrifuge
  7. Kev tuav kua (xaiv)
  8. Cassette case processor (xaiv)

Cov txheej txheem tsim khoom hauv kev txhim kho immunochromatography

  1. Colloidal kub npaj
  2. Colloidal kub labeling
  3. Dispensing antigen los yog antibody rau Nitrocellulose daim nyias nyias
  4. Nitrocellulose membrane lo rau daim npav thaub qab
  5. Laminate conjugate ncoo ntawm daim npav thaub qab
  6. Laminate qauv ncoo ntawm daim npav thaub qab
  7. Laminate nqus ncoo ntawm daim npav thaub qab
  8. Luam Logo daim ntawv lo rau ntawm lub ncoo nqus (xaiv)
  9. Txiav cov daim ntawv tsis txiav nyob rau hauv strips
  10. Muab cov hlua tso rau hauv lub hnab ntawv lossis lub raj mis
  11. Ntim hauv lub thawv

Xav paub ntau ntxiv, thov mus saib Yuav Ua Li Cas Txhim Kho Kev Ntsuam Xyuas Kev Ntsuas Siab?
Yuav ua li cas kom siv lub rooj sib txoos rau qhov ntsuas nrawm?

Yuav siv colloidal kub immunochromatography rau zaub mov kev ruaj ntseg nrhiav tau?

Colloidal kub immunochromatography dav siv hauv kev sim zaub mov rau ntau xyoo. Piv txwv li, ua li cas kuaj mis ua haujlwm ntawm tshuaj tua kab mob seem hauv cov mis nyuj?

Cov tshuaj tua kab mob yog cov tshuaj me me, yog li, qhov kev ntsuam xyuas xaiv txoj kev sib tw. Cov tshuaj tiv thaiv kab mob yog dab tsi?

Cia peb pom cov qauv kab txaij:

lateral-flow-assay
lateral-flow-assay
kub-nanoparticles
kub-nanoparticles

Cov colloidal kub sau cov tshuaj tiv thaiv kab mob lyophilizated hauv zoo daim hlau. Kev sib cais los ntawm kab txaij, ua kom lub sijhawm tsim kom loj hlob thiab txhim kho kev paub qhov tseeb.

Lateral flow reaction mechanism

sab-ntws-tshuab
sab-ntws-tshuab
immunochromatography
immunochromatography

Hauv Cov Qauv Zoo:

  1. Nqa cov mis nyuj nyoos thiab ntxiv rau hauv lub qhov dej me
  2. Yog tias cov mis nyuj muaj cov tshuaj me me, xws li, beta lactam, tetracycline, chloramphenicol, Sulfonamides, Cephalosporins, Melamine, thiab lwm yam ....
  3. Cov tshuaj tiv thaiv kub-lo ntawv kub (Au-Ab) hauv cov qhov dej me me yuav ntes cov lwg me me los ntawm cov qauv mis, dhau los ua qhov tshiab (Au-Ab khi nrog cov qauv me me).
  4. Thaum txoj kev txav mus rau txoj kab T txoj haujlwm, tsis muaj txoj haujlwm rau BSA-antigen (cov tshuaj tiv thaiv kab mob uas tsau rau T lin) kom sib txuas, vim tias kub-nanoparticle twb tau sib txuas nrog cov molecules me me, yog li, cov xim xim yuav tsis hloov.
  5. Qhov tshuaj tiv thaiv kab mob thib ob uas tsau rau ntawm kab C yuav ntes cov ntawv kub-cim cov tshuaj tiv thaiv kab mob thiab cov xim pom.
  6. Qhov tshwm sim yuav zoo.

Hauv Cov Qauv Tsis Zoo:

  1. Daim ntawv lo-tiv thaiv kab mob kub (Au-Ab) tsis tuaj yeem ntes cov molecules me me los ntawm cov mis nyuj nyoos, tab sis yuav raug ntes los ntawm T kab cov kab mob antigen, tom qab ntawd cov xim band pom.
  2. C kab's cov kab mob tiv thaiv kab mob tseem tseem khaws cov kub-lo lo lus tshuaj tiv thaiv kab mob (Au-Ab), cov xim xim pom.

Kev txhais lus

Txoj Kev Colorimetric rau kev txhais lus ntawm kev kuaj mis. Kab ntawv sib piv nrog kab tswj los txiav txim siab qhov ua tau zoo lossis tsis zoo. Thaum tsim khoom los tswj kom muaj nuj nqis ntawm Au-Ab kom ua tiav kev ntsuas xim.

xaus

Immunochromatography yog qhov txuj ci thev naus laus zis nrov heev, siv dav hauv cov khoom kuaj mob vitro, tsis yog siv hauv kev sim zaub mov. Qhov sib txawv ntawm kev ntsuas ib sab, colloidal kub immunochromatography kuaj tsis xav tau cov cuab yeej siv kim, eays siv, tus nqi pheej yig los pab tib neeg kom nyob tau zoo dua, piv txwv li, kuaj cev xeeb tub hauv tsev.

reference:

Yuav Pib Li Cas Tsim Cov Khoom Siv Covid 19

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