Aflatoxin, ua mycotoxin, yog cov tshuaj metabolite ntawm cov fungi tsim thaum cov qoob los yog zaub tshiab tsis qhuav thiab khaws cia hauv lub sijhawm, thiab muaj nyob thoob plaws. Vim tias nws muaj tshuaj lom hnyav thiab ua rau muaj qog noj ntshav, cov kev cai nruj ntawm nws tau teev tseg hauv cov qauv zaub mov hauv tebchaws thiab cov qauv pub zaub mov. Aflatoxin M1 Cov belongs rau mycotoxin thiab yog hydroxylation metabolite ntawm aflatoxin B1 hauv tsiaj. Nws yog tshuaj lom thiab carcinogenic, yuav tsum yog tiv thaiv los ntawm kev ntxiv rau zaub mov. Vim nws yog teeb meem rau daim siab thiab tuaj yeem ntxias mis tsis haum thiab mob qog noj ntshav, nws tau teev tseg ua carcinogen los ntawm LEEJ TWG Cancer Research Institute thaum ntxov li xyoo 1993.
Vim tias cov ntsiab lus qis thiab cov qauv zoo sib xws ntawm aflatoxin, nws xav tau kev nkag siab siab thiab qhov tshwj xeeb ntawm txoj hauv kev tshawb nrhiav, uas suav nrog kev sib cais thiab kev txheeb xyuas. Hauv daim ntawv no, ua haujlwm siab ua kua chromatography (HPLC) tau siv rau txheeb xyuas aflatoxin. Qhov teeb meem ntawm cov ntsiab lus qis ntawm aflatoxin tau kov yeej, thiab qhov ua tau zoo siab tau txais.
Qauv ua ntej kho
Cov mis nyuj los ntawm mis nyuj lag luam tau noj 5g thiab muab tso rau hauv 100mL ntsuas lub raj mis. Cov mis hmoov tau yaj los ntawm kev ntxiv 20 mL dej sov ntawm 50 degrees Celsius kom nws oscillate rau 30S. Cov tshuaj sodium chloride tau ntxiv los ua kom nws yaj los ntawm kev sib xyaw rau 2g. 50 mL acetonitrile tau ntxiv rau oscillate rau 30S. Tom qab sib tov, acetonitrile tau ntxiv rau hauv lub raj mis kom tau raws li qhov ntsuas. Cov kab oscillation ua rau nws homogeneous. Pom, 40 mL ntawm cov lim dej, 40 C nitrogen ntws mus rau qhov ze qhuav. Qhov seem tau yaj los ntawm kev ntxiv 20 ml 10% acetonitrile. Cov kab sib txheeb tau raug ntxuav nrog 10 mL dej tom qab cov qauv dhau los ntawm kab ntawv kev sib raug zoo tag.
Thaum kawg, 2 mL acetonitrile tau siv rau kev tshem tawm. 1.5 ml ntawm eluent tau sau thiab qhuav nrog nitrogen. Ntxiv 200 uL hexane thiab 200 uL trifluoroacetic acid, co thiab sib tov, ua rau kub 40 C rau 10 feeb, tshem tawm, ziab hauv qab nitrogen ntws, ntxiv 10% acetonitrile 0.5 ml, co rau 10 vib nas this.
sim mob
Ntsuas hom: Dionex Ultimate 3000 Series
Twj: LPG-3400SD
Tsis Siv Neeg Sampler: WPS-3000SL
Kem Lub Thawv Kub: TCC-3000RS
Ntes: FLD
Chromatography Software: Chromeleon Chromatography Cov Ntaub Ntawv
Ntes hom, tsis ua haujlwm, thiab S/N tus lej:
Fluorescence detector, FLD-3400
Lub sijhawm teb = 1
FLD FlowCell Kub Nominal = 35.00 [C]
FLD FlowCell ReadyTempDelta = 1.00 [C]
BaselineBehavior = Ntxiv mus
Cov Ntaub Ntawv Sau Tus Nqi Nominal = 10.00 [Hz]
Emission 1.ExWavelength = 365.0 [nm]
Kev Tso Tawm 1. Em Wavelength = 435.0 [nm]
Kev tso tawm 1. rhiab heev = 6
Emission 1.PMT = Pib
Emission 1. FilterWheel = Pib
Chromatographic tej yam kev mob:
Chromatographic Kem:
Thermo Scientific Hypersil GOLD C18, 5 um, 100 x 2.1 mm No. 25005-102130
Txawb theem:
Sampling ntim: 20 mL
Txaus tshaj tawm: 800 mL/min
Sab kub: 25 C
QHOV ZOO TSHAJ PLAWS
Daim duab 1 Piv txwv kev txiav txim siab chromatogram (1 ppb ntxiv rau cov qauv chromatogram; 2 tus qauv dawb chromatogram)
Hauv daim ntawv no, Aflatoxin B1, B2, G1, G2, M1 hauv cov noob hnav tau tshuaj xyuas los ntawm kev siv Thermo Scientific Hypersil GOLD C18, 5 microns, 100 *2.1 mm kem, ua ntej kab ntawv sib xyaw ua ke nrog lub ntsuas hluav taws xob. Qhov zoo tshaj plaws, kev sib cais thiab kev nkag siab siab tau tshwm sim.
Yog xav paub ntau ntxiv txog cov khoom siv tshuaj mycotoxin, thov nyem no.