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Kev Ntsuas Quinolones

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Quinolones

Kev xeem Quinolones yog dab tsi?

Kev Ntsuas Quinolones yog ua raws Colloidal Gold Immunochromatography txhawm rau txheeb xyuas Quinolones cov khoom seem hauv cov khoom zib ntab. Cov khoom siv no tuaj yeem thov rau ntawm qhov chaw nrawm lossis kuaj sim los ntawm ntau lub chaw haujlwm.
Tau Txais Tus Nqi Pub Dawb!

Quinolones yog dab tsi?

1. Txhais

Quinolones (4-quinolones), tseem hu ua pyridonic acid lossis pyridonic acid, yog chav kawm ntawm cov tshuaj tua kab mob tua kab mob. Quinolones yog cov tshuaj tua kab mob uas feem ntau ua rau cov kab mob Gram-tsis zoo, thiab muaj qhov cuam tshuam tsis zoo rau cov kab mob Gram-positive (qee qhov ntau yam muaj cov tshuaj tua kab mob zoo dua ntawm Staphylococcus aureus).

Cov tshuaj tua kab mob Quinolone yog cov tswv cuab ntawm ntau cov tshuaj tua kab uas nthuav dav uas sib koom cov qauv txheej txheem bicyclic cuam tshuam nrog 4-quinolone tshuaj. Lawv tau siv hauv tib neeg thiab tsiaj kho tsiaj los kho kab mob raws li kev ua tsiaj txhu.

Yuav luag tag nrho cov tshuaj tua kab mob quinolone siv yog fluoroquinolones, uas muaj cov tshuaj fluorine hauv lawv cov qauv tshuaj thiab muaj txiaj ntsig zoo tiv thaiv kab mob Gram-negative thiab Gram-positive. Ib qho piv txwv yog ciprofloxacin, uas yog ib qho ntawm cov tshuaj tua kab mob siv ntau tshaj plaws hauv ntiaj teb.

2. Qauv

Lub hauv paus pob txha ntawm cov tshuaj tua kab mob quinolone muaj cov qauv nitrogen (hetero) biparacyclic. Quinolone thiab lwm yam tshuaj tua kab mob muaj cov ntsiab lus sib txawv ntawm kev nqis tes ua. Lawv tsom cov kab mob DNA.

3. Lub tshuab ua haujlwm

Lub hauv paus pob txha ntawm cov tshuaj tua kab mob quinolone muaj cov qauv nitrogen (hetero) biparacyclic. Quinolone thiab lwm yam tshuaj tua kab mob muaj cov ntsiab lus sib txawv ntawm kev nqis tes ua. Lawv tsom cov kab mob DNA (DNA). 

Qhov ob-kab DNA ntawm cov kab mob tau sib tw ua cov maggot lossis kauv (hu ua supercoil). Cov enzyme uas ua DNA rau hauv supercoil hu ua DNA gyrase. Quinolones cuam tshuam cov enzyme no thiab ntxiv ua rau tsis tuaj yeem ua puas rau cov kab mob DNA. Cov hlwb tsis faib ntxiv lawm. 

Lawv qhia pom cov tshuaj lom rau cov kab mob. Tam sim no, qhov tsis kam ntawm qee cov kab mob rau ntau yam tshuaj tua kab mob tuaj yeem nthuav dav vim yog kis plasmid. Cov tshuaj hauv chav kawm no tsis cuam tshuam los ntawm plasmid-kis tsis kam, yog li tsis muaj kev tiv thaiv kab mob sib kis ntawm cov tshuaj no thiab ntau yam tshuaj tua kab mob.

4. Kev faib tawm

Quinolone tau muab faib ua thawj, thib ob, thib peb, thiab plaub tiam raws li kev tsim thiab nws cov tshuaj tua kab mob.

Thawj-tiam quinolones muaj cov tshuaj tua kab mob tsuas yog rau E. coli, Shigella, Klebsiella, thiab ob peb Proteus. Ntau yam tshwj xeeb suav nrog Nalidixic acid thiab Piromidic acid, thiab lwm yam, uas tsis tshua muaj siv vim qhov ua tsis tau zoo.

Lub cim thib ob quinolones tau nthuav dav hauv cov kab mob tua kab mob thiab muaj qee yam tshuaj tua kab mob rau Enterobacter, Citrobacter, Pseudomonas aeruginosa, thiab Serratia. Pyridenic acid yog daim ntawv thov tseem ceeb hauv tsev. Ib qho ntxiv, tseem muaj Cinoxacin thiab Miloxacin, uas tau tsim tawm txawv teb chaws.

Cov kab mob tua kab mob ntawm cov cim thib peb quinolones tau nthuav dav ntxiv. Nws kuj muaj cov tshuaj tua kab mob rau cov kab mob Gram-positive xws li staphylococci, thiab ntxiv dag zog rau cov kab mob tiv thaiv kab mob rau qee cov kab mob Gram-negative. Ntawm chav kawm tshuaj no, norfloxacin tau tsim tawm hauv Suav teb. Tseem muaj Ofloxacin, Perfloxacin, Enoxacin, Ciprofloxacin thiab lwm yam. Cov tshuaj tiam no muaj cov tshuaj fluorine hauv cov qauv. Nws yog li ntawd hu ua fluoroquinolone.

Plaub tiam quinolones tau hloov kho tus qauv piv rau peb tiam dhau los ntawm cov tshuaj. Kev qhia txog 8-methoxy hauv cov qauv pab ua kom muaj zog tiv thaiv kev ua kom tsis muaj zog, thaum cov txheej txheem nitrogen-dioxy ring ntawm C-7 txoj haujlwm ntxiv dag zog rau kev tiv thaiv kab mob gram zoo thiab ua thawj qhov haujlwm tiv thaiv kab mob Gram-negative, kev phom sij me dua, tab sis tus nqi kim dua. Nws tau txhim kho kev ua haujlwm tiv thaiv kab mob tiv thaiv kab mob Gram-positive, txhim kho kev cuam tshuam ntawm cov kab mob anaerobic suav nrog Bacteroides tsis yooj yim, thiab txhim kho cov txiaj ntsig ntawm cov kab mob zoo xws li Mycoplasma mob ntsws, Chlamydia pneumoniae, Legionella thiab Mycobacterium tuberculosis. Cov khoom lag luam feem ntau tau ncua ib nrab ntawm lub neej, xws li Gatifloxacin thiab Moxifloxacin.

Kev phom sij ntawm Quinolones

Cov tshuaj Alkaline, tshuaj tiv thaiv kab mob anticholinergics, thiab H2 receptor blockers tuaj yeem txo qis cov kua qaub hauv plab thiab txo qis kev nqus ntawm cov tshuaj no. Tsis txhob coj lawv ua ke.

Rifampicin (RNA synthesis inhibitor) thiab chloramphenicol (protein synthesis inhibitor) tuaj yeem txo qhov kev cuam tshuam ntawm chav kawm ntawm cov tshuaj no, tshem tawm cov teebmeem ntawm nalidixic acid thiab norfloxacin, thiab ua oxazine thiab Cov nyhuv ntawm ciprofloxacin yog ib nrab.

Fluoroquinolone inhibits theophylline metabolism. Thaum nws tau siv ua ke nrog theophylline, cov ntshav cov ntshav ntawm theophylline tau nce, thiab theophylline cov tshuaj lom yuav tshwm sim, uas yuav tsum tau ua tib zoo mloog.


Cov tshuaj tiv thaiv tsis haum xeeb loj ntawm cov tshuaj quinolone yog: lub zos liab, o, ua pob, ua xua, thiab ua rau lub ntsej muag ci. Ntawm cov kev tsis haum tshuaj, kev poob siab anaphylactic yog qhov phom sij tshaj plaws rau cov neeg mob, thiab tseem ua rau tuag taus.

Nta ntawm Quinolones Test

1. 15 feeb nyeem cov txiaj ntsig

2. Kev txwv tsis pub teb rau EU thiab Asmeskas FDA cov cai

3. Tsis yog cov kws tshaj lij lossis cov cuab yeej xav tau

4. Yooj yim rau kev khiav lag luam thiab nyeem
Kev ntsuam xyuas Quinolones

Kev Tshawb Nrhiav Txoj Kev Xeem Quinolones

Kev Ntsuas Quinolones, raws li txheej txheem kev sib tw colloidal kub, yog siv rau kev ntsuas Quinolones seem hauv cov qauv zib ntab thiab ua tau raws li EU MRL. Cov khoom siv no tuaj yeem thov rau ntawm qhov chaw kuaj los ntawm ntau lub chaw haujlwm.

Piv txwv ntawm Quinolones Test

Piv txwv muaj xws li: zib ntab

Kev Txiav Txim Txwv ntawm Quinolones Test

LOD tuaj yeem hloov pauv raws li qhov xav tau lossis qhov txawv ntawm qhov sib txawv, thov mus saib hauv phau ntawv qhia kom paub meej ntxiv.

Cov Ntaub Ntawv Kev Ua Haujlwm

FluoroquinolonesMRL (ug / kg)
Enrofloxacin0.5
Ciprofloxacin1
Ofloxacin0.5
Flumequine1
Danofloxacin0.5
Norfloxacin0.5
Oxilinic acid1
Lomefloxacin5
Gatifloxacin9
Enoxacin7
Levofloxacin7.5
Danofloxacin5
Pefloxacin5
Sarafloxacin2

Qhov tseem ceeb ntawm Quinolones Test

Kev ntsuas Quinolones tuaj yeem pab cov tuam txhab tsim khoom, cov koom haum tswj hwm, thiab pej xeem saib xyuas cov khoom lag luam zoo thiab pab tib neeg tiv thaiv lawv txoj kev noj qab haus huv.

Cheebtsam ntawm Quinolones Test

1. Cov Khoom Ntsuas: 10pcs

2. Hexane (12ml): 1 lub raj mis

3. Khoom Qhia: 1

4. Micro zoo phaj: 10 npx

5. Re-Hydration Fluid (2ml): 1 lub raj mis

6. Centrifuge Tube (15ml): 20pcs

Cov ntaub ntawv rau Kev Ntsuas Quinolones

1. Ethyl Acetate

2. Tov khoom

3. Oscillator

4. Kev Ntsuas Hluav Taws Xob

5. Centrifuge

6. Organomation

7. Pipette

Yuav siv Quinolones Test li cas?

1. Nyeem cov lus qhia thiab cia tus qauv ntawm chav sov (20-25 ℃).

2. Tshem tawm cov ntawv xeem, siv tsis pub dhau 1h.

3. Muab 100µL cov qauv coj mus tso rau hauv lub qhov dej micro, xa dej mus rau thiab nqis rau 6 zaug kom yaj cov tshuaj reagent. Tom qab ntawd, muab tso rau 5 feeb ntawm chav sov.

4. Tso 100µL cov qauv rau hauv cov qauv zoo, sawv ntsug rau 5mins ntawm chav sov.

5. Nyeem qhov tshwm sim tsis pub dhau 5-8mins. Qhov tshwm sim tom qab 5-8 feeb tsis raug.

Kev txhais lus rau Quinolones Test

Kev txhais lus rau kev xeem

Ntsiab lus

Thaum tshuaj tua kab mob tau siv thawj zaug rau tib neeg, peb suav tias lawv yog "elixir". Raws li qhov teeb meem ntawm kev siv tshuaj tua kab mob txuas ntxiv mus zuj zus, lawv qhov kev tiv thaiv kuj tseem ua rau muaj kev puas tsuaj loj rau tib neeg.

Yog li, hauv lub neej txhua hnub, peb yuav tsum zam zaub mov nrog tshuaj tua kab mob seem. Thawj kauj ruam rau kev pab tus kheej: peb yuav tsum kuaj cov tshuaj tua kab mob seem.

Yuav ua li cas xaj Quinolones Test?

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