Enterobacter sakazakii fluorescence kev kuaj PCR lub sijhawm tiag

Lub sijhawm tiag tiag fluorescent PCR hauv vitro kuaj pom ntawm enterobacter sakazakii.

Kev qhia tshwj xeeb: 96 T / lub thawv
Cov khoom siv tau khaws cia ntawm 2 - 8 ℃.
Lub sijhawm siv tau yog 12 lub hlis.

specification: 96T / lub thawv

Siv: Lub sijhawm tiag tiag fluorescent PCR hauv vitro kuaj pom ntawm enterobacter sakazakii.

Txoj cai tshawb nrhiav: Cov khoom siv muaj cov kab mob enterobacter sakazakii tshwj xeeb. Thaum cov qauv muaj enterobacter sakazakii, enterobacter sakazakii DNA muab rho tawm tom qab ua ntej kev txhim kho tau nthuav dav los ntawm PCR. Thaum lub sijhawm tshuaj tiv thaiv, enterobacter sakazakii-tshwj xeeb fluorescent sojntsuam sib xyaw nrog lub hom phiaj nucleic acid thiab ib txhij taq enzyme Hydrolysis txhawm rau cais cov pab pawg fluorescent los ntawm pab pawg quenching ntawm kev sojntsuam: yog li muaj cov enterobacter sakazakii tuaj yeem txiav txim hauv lub sijhawm tiag tiag qhov nce ntawm fluorescence.

Khoom thiab muaj pes tsawg leeg:

Muaj pes tsawg leeg (96T/khoom siv)	specification	kom muaj nuj nqis
Kev daws cov tshuaj DNA	3ml	1 raj
Enterobacter sakazakii PCR cov tshuaj tiv thaiv	1100ul	1 raj
Kais enzyme (5U/ul)	20ul	1 raj
Enterobacter sakazakii tswj tau zoo	40ul	1 raj
Enterobacter sakazakii Kev Tswj Tsis Zoo	0.5ml	1 raj
phau ntawv qhia		1

Cov xwm txheej cia thiab hnub tas sij hawm ntawm cov khoom siv:
1. Cov khoom siv tau khaws cia ntawm 2 -8 ℃.
2. Lub sijhawm siv tau yog 12 lub hlis.

Cov cuab yeej siv tau:
ABI7500, 9600 series, MJ opticon2, Bio-Rad thiab lwm yam fluorescent ntau qhov ntsuas PCR muaj

Txoj kev sim:
1. Siv 1ml ntawm cov tshuaj ua kom zoo rau hauv 1.5m1 cov menyuam tsis muaj menyuam centrifuge raj, centrifuge ntawm 10.000rpm rau 5min, thiab pov tseg cov supernatant; siv cov txheej txheem rho tawm phenol chloroform qub los kuaj cov ntawv rho tawm lossis khaws nws ntawm 20C kom txog thaum nws raug sim.
2. Muab PCR cov tshuaj tiv thaiv ntawm chav sov kom sib npaug, coj Taq enzyme tom qab yaj, thiab ntxiv Taq enzyme ntawm 2∪/raj, uas yog, txhua qhov kev sim tshuaj tiv thaiv kab mob tau npaj ua: PCR cov tshuaj tiv thaiv tshuaj 22.5u1+0.4ulTaq enzyme.
3. Ntxiv tus qauv: Thaw cov nucleic acid extract khaws cia ntawm -20 ° C ntawm chav sov thiab centrifuge ntawm 13,000 rpm rau 5 feeb. Qhov tsis zoo thiab kev tswj hwm tau yaj hauv chav sov ua ntej siv. Hauv txhua lub PCR cov tshuaj tiv thaiv raj, ntxiv 2 ul ntawm tus qauv lossis qhov tsis zoo thiab kev tswj hwm tau ua tiav hauv qib 1, npog lub raj, tso rau ntawm PCR ntsuas, thiab sau tus lej piv txwv.
4. On-board amplification thiab nrhiav chaw: qhov xwm txheej tshwm sim yog 95C: 3min, 1 lub voj voog; 95C: 5sec, 60C: 40sec (teeb liab tau txais), 40 mus. Cov tshuaj tiv thaiv kab mob tau teeb tsa rau 25u1.
Rau ntau-channel fluorescence PCR ntsuas, lub teeb liab tau teeb tsa rau Fam fluorescein.

Cov txiaj ntsig kev txiav txim:
Thov mus saib phau ntawv qhia kom ntxaws.

Ceev faj rau kev siv cov khoom siv:
1. Cov khoom siv no tsuas yog siv rau hauv vitro kuaj thiab tshawb fawb lub hom phiaj, thov nyeem tag nrho cov ntawv ntawm phau ntawv no ua tib zoo ua ntej pib siv nws.
2. Kev sim yuav tsum tau muab faib nruj me ntsis: thaj tsam npaj ua ntej PCR-npaj cov tshuaj reagents; cheeb tsam ua qauv-yuav tsum kuaj cov qauv, ua qauv; thaj tsam nrhiav pom-PCR kev nthuav dav, nrhiav pom.
3. Rau cov txheej txheem kev tswj hwm hauv chaw sim, thov saib mus rau qhov ua tiav ntawm cov qauv kev tswj hwm rau kev hloov pauv cov noob tso tawm los ntawm cov koom haum hauv tebchaws.

Yog tias koj xav paub ntau ntxiv, thov hu rau peb

Cov lus nug tsis tseem ceeb: Enterobacter sakazakii

Txog enterobacter sakazakii

Enterobacter sakazakii yog dab tsi

Enterobacter tus kab mob (ES) yog Gram-negative non-Bacillus parasitic hauv tib neeg thiab tsiaj txhu, koom nrog tsev neeg Enterobacteriaceae. Lawv tuaj yeem ua rau muaj kab mob rau tib neeg thiab tsiaj txhu raws li qee yam xwm txheej, yog li lawv raug hu ua tus kab mob raws cai.

Kev phom sij ntawm enterobacter sakazakii

Enterobacter sakazakii tuaj yeem ua rau mob hnyav rau menyuam mos, mob enterocolitis, kab mob bacteremia, thiab ua haujlwm tsis zoo ntawm lub paj hlwb. Xyoo 1961, Tebchaws Askiv tau tshaj tawm thawj ob kis mob meningitis los ntawm Enterobacter sakazakii. Tom qab ntawd, Tebchaws Asmeskas, Tebchaws Netherlands, thiab Canada thiab lwm lub tebchaws tau tshaj tawm qhia txog tus kabmob neonatal Enterobacter sakazakii. Cov menyuam mos uas muaj tus kab mob Enterobacter sakazakii muaj neeg tuag coob txog li 20%-50%, thiab tseem muaj menyuam mos thiab cov menyuam yaus tseem muaj sia nyob kuj tseem yuav muaj cov kab mob hauv lub cev tsis zoo.

Kab mob Enterobacter sakazakii

Xyoo 1961, ob tus kws tshawb fawb, Urmenyi thiab Franklin hauv tebchaws Askiv tau tshaj tawm thawj zaug ob kis mob meningitis tshwm sim los ntawm cov kab mob, tom qab ntawd mob meningitis, septicemia, thiab necrotizing cov hnyuv me uas tshwm sim los ntawm Enterobacter sakazakii ua tiav cov lus ceeb toom thoob ntiaj teb.

Tus kab mob Enterobacter sakazakii hauv tus menyuam mos

Cov xwm txheej sib kis thiab sib kis ntawm tus mob meningitis, septicemia, thiab necrotizing colitis tshwm sim los ntawm Enterobacter sakazakii hauv cov menyuam mos thiab menyuam mos yug ntxov tau tshwm sim tom qab lwm qhov hauv ntiaj teb. Ntau qhov kev tshawb fawb tau qhia tias cov menyuam mos mis yog tam sim no yog cov tseem ceeb ntawm kev kis tus kab mob ua rau mob meningitis, sepsis thiab necrotizing colitis hauv cov menyuam mos thiab menyuam mos liab. Hauv qee kis, kev tuag los ntawm tus kab mob tshwm sim los ntawm Enterobacter sakazakii tuaj yeem ncav cuag 40%～ 80%. Enterobacter sakazakii tau nyiam cov saib xyuas ntawm cov haujlwm hauv ntau lub tebchaws thoob ntiaj teb.

Cov tsos mob Enterobacter sakazakii

Cov menyuam yaus feem ntau muaj cov tsos mob me me thiab tsis zoo thiab pom tau yooj yim. Cov mob hnyav tuaj yeem ua rau mob necrotizing enterocolitis, sepsis, thiab meningitis.
(1) Cov tsos mob ntawm lub cev: ua npaws. Cov menyuam mos liab tuaj yeem pom cov cim ntawm kev kub siab, kev nyuaj siab hauv lub cev, kev tsis lees paub mis, nce daj ntseg, tawv nqaij tawv, tawv nqaij thiab ua rau poob siab.
(B) Cov tsos mob ntawm lub plab zom mov: ntuav, ntuav hauv plab, raws plab, tso quav tso zis tso quav, lub plab tsis muaj zog lossis txawm tias ploj mus, thaum muaj mob hnyav, tuaj yeem ua rau mob plab thiab mob peritonitis.
(3) Cov tsos mob ntawm lub paj hlwb: yws yws, quaj ntsiag to, tsaug zog thiab txawm tias tsis nco qab, ntsia, ntuav tuaj yeem tshwm sim, kev tshuaj xyuas lub cev tuaj yeem suav nrog lub taub hau loj dua ib puag ncig, cais craniotomy, ua rau muaj zog ntxiv, thiab ua rau lub cev tsis zoo.

Enterobacter sakazakii kev kho mob

(1) Cov mob me me yuav tsum tau ntxiv dag zog thiab kho tus tsos mob kom ntseeg tau tias tau txais cov kua thiab electrolytes, thiab ua tib zoo saib xyuas ntxiv.
(2) Cov neeg uas muaj cov tsos mob hauv plab yuav tsum xaiv cov tshuaj tua kab mob. Amoxicillin/clavulanic acid thiab lwm yam tshuaj tua kab mob tuaj yeem xaiv thiab kho raws li cov txiaj ntsig ntawm kev sim tshuaj tiv thaiv kab mob. Cov tshuaj tiv thaiv kab mob plab hnyuv thiab cov kab mob me me tuaj yeem siv tib lub sijhawm.
(3) Hauv cov xwm txheej hnyav, ceftazidime, cefepime, melopenem, thiab lwm yam tuaj yeem siv rau kev kho cov kab mob tua kab mob, thiab kho raws li cov txiaj ntsig kev kho mob hauv tsev thiab cov txiaj ntsig kev sim tshuaj. Kev kho mob raws kev kho mob yuav tsum ua kom muaj zog; cov menyuam yaus uas muaj tus kabmob necrotizing enterocolitis yuav tsum tau yoo mov, lub plab zom zaws yuav tsum tau muab rau cov uas mob plab hnyav, thiab yuav tsum tau kho kev phais yog tsim nyog; menyuam yaus uas muaj tus mob meningitis yuav tsum tau muab sedation, txo kev ntxhov siab vim, txo qis hauv siab thiab teeb meem Kev kho; muab tshuaj tiv thaiv kev poob siab nyob rau lub sijhawm thaum muaj kev poob siab.

Yuav kis tus kab mob enterobacter sakazakii li cas

Enterobacter sakazakii yog Enterobacteriaceae, Gram-negative bacilli. Enterobacter sakazakii tau nthuav dav dav hauv qhov xwm txheej thiab tsim tawm sai, tab sis nws tsis tiv taus cua sov thiab tuaj yeem tua los ntawm kev muab tshuaj tua kab mob. Enterobacter sakazakii yog ib hom kab mob uas ua rau muaj mob. Raws li qhov xwm txheej ib txwm muaj, nws tsis ua teeb meem rau tib neeg kev noj qab haus huv, tab sis nws tuaj yeem ua rau muaj kab mob rau cov tib neeg uas tsis muaj kev tiv thaiv kab mob tsawg thiab menyuam mos, cov menyuam yug tshiab, tshwj xeeb yog cov menyuam mos ntxov thiab cov menyuam tsis muaj qhov hnyav.

Enterobacter sakazakii cov khoom noj muaj tshwm sim

Khoom noj thiab ib puag ncig yog lub hauv paus tseem ceeb ntawm E. sakazakii kev kis kab mob. Cov kws tshawb fawb tau cais cov kab mob enterobacter sakazakii los ntawm ntau yam zaub mov thiab dej haus. Ntau yam khoom siv raw ntawm cov khoom noj no thiab dej haus, xws li mis hmoov, khoom siv nqaij, cov khoom ua kua, qhob cij, thiab lwm yam, tuaj yeem kis tus kab mob enterobacter sakazakii, ua rau pom cov kab mob hauv cov khoom kawg. Ntawm lawv, cov menyuam mos mis mis hmoov yog hu ua cov khoom noj feem ntau thiab rhiab tshaj plaws ntawm enterobacter sakazakii.

Enterobacter sakazakii hauv cov mis mos ua hmoov

Enterobacter sakazakii yog tsiaj txhu uas muaj nyob hauv ib puag ncig thiab feem ntau pom muaj nyob hauv cov tsev thiab ib puag ncig hauv tsev uas cov mis mos tau tsim. Txawm hais tias tus menyuam mos mis tsis yog cov khoom lag luam uas tsis muaj menyuam thiab tej zaum yuav kis tau cov kab mob uas tuaj yeem ua rau muaj kab mob loj, yog tias tus menyuam mos mis tau npaj thiab khaws cia kom raug, kev pheej hmoo kis mob los ntawm Enterobacter sakazakii tuaj yeem txo qis. Txhawm rau npaj cov mis mos kom nyab xeeb, koj yuav tsum xub ua kom huv thiab tsis muaj menyuam kom pub mis thiab npaj khoom siv.

Kev tiv thaiv Enterobacter sakazakii

Txog kev sib kis ntawm Enterobacter sakazakii hauv cov zaub mov menyuam mos, kev kis kab mob sib kis yuav tsum raug tswj los ntawm qhov chaw, ua haujlwm thiab ntim cov ntaub ntawv ntawm cov zaub mov menyuam mos. Ua ntej tshaj plaws, peb yuav tsum ntxiv dag zog tiv thaiv kev ua qias tuaj thiab tswj cov khoom noj rau menyuam mos thiab menyuam yaus. Kev tua kab mob nruj me ntsis thiab tshuaj xyuas cov khoom siv raw yuav tsum tau ua. Cov ntaub ntawv raw khoom uas tsis huv yuav tsum tau ua tiav raws sijhawm. Tsis txhob nkag mus rau qhov chaw tsim khoom kom tsis txhob muaj kuab paug rau lwm cov khoom siv raw.

Qhov thib ob, nruj me ntsis sterilize txhua qhov txuas hauv cov txheej txheem, xws li tus neeg teb xov tooj lub qhov ncauj, txhais tes, taw, taub hau, khau, ua haujlwm khaub ncaws, lub kaus mom, thiab lwm yam, yuav tsum ua kom huv si. Nyob rau tib lub sijhawm, cov kab mob tau raug tua tag nrho los ntawm kev muab tshuaj tua kab mob, ua kom sov kom sov kom siab lossis lwm yam txheej txheem kub thaum ua haujlwm.

Txhawm rau tiv thaiv E. sakazakii kev sib kis, kev tshuaj xyuas kev phom sij zoo thiab kev tswj hwm lub zog rau cov ntsiab lus tswj hwm tseem yuav tsum tau tsim thiab siv nruj me ntsis thaum tsim cov txheej txheem. Ib qho ntxiv, cov ntaub ntawv ntim khoom yuav tsum tau muab tshuaj tua kab mob kom zoo, thiab txhua qhov chaw ua haujlwm tsim khoom thiab ntim khoom yuav tsum tau muab tshuaj tua kab mob tas li.

Ib qho ntxiv, hauv kev pub mis rau menyuam mos thiab menyuam yaus, vim tias tseem muaj kev pheej hmoo kis mob Enterobacter sakazakii hauv tsev neeg, nws raug nquahu kom fwj thiab lwm yam tais diav rau mis nyuj hmoov kom huv thiab ua kom huv. Ib qho ntxiv, kev pub niam mis kuj tseem yog ib txoj hauv kev muaj txiaj ntsig zoo los txo qhov kev pheej hmoo ntawm kev yug menyuam mos nrog E. sakazakii.

Enterobacter sakazakii kev txheeb xyuas biochemical

Kev tshuaj xyuas ib txwm muaj

Cov txheej txheem ib txwm muaj kev sib cais thiab kuaj pom ntawm Enterobacter sakazakii nyob ntawm cov yam ntxwv biochemical thiab morphological. Thoob ntiaj teb, "Kev cais thiab suav ntawm Enterobacter sakazakii hauv cov mis mos mos" tshaj tawm los ntawm Asmeskas FDA feem ntau yog siv raws li txheej txheem qub.

Matrix-pab laser desorption ionization lub sij hawm-ntawm-dav hlau huab hwm coj spectrometry

Matrix-pab laser desorption ionization time-of-flight mass spectrometry (MAL-DITOF-MS) txoj kev yog sib xyaw cov qauv coj los ntsuas nrog cov tshuaj matrix ntawm qee qhov sib piv, thiab siv cov tshuab hluav taws xob pulsed kom ua rau lub matrix, ua rau polymer los tsim cov tshuaj molecular ions thiab molecular ion polymerization Lub cev tau nrawm dua thiab kuaj pom nyob rau lub sijhawm-ntawm-kev ya dav hlau loj spectrometer. Raws li qhov tshwm sim, cov ntsuas polymer tau sib cais raws li tus lej loj, ua tiav los tsim cov pawg loj, thiab tshuaj xyuas thiab sib piv los ntawm software los tshuaj ntsuam thiab txiav txim siab qhov tshwj xeeb (ntiv tes) spectrum kom ua tiav qhov sib txawv thiab txheeb xyuas cov hom phiaj kab mob me me lossis hom.

Molecular biology xeem txoj kev

Tam sim no, txheej txheem kev sim tshuaj lom neeg molecular ntawm E. sakazakii siv hauv cov khoom lag luam mis nyuj suav nrog cov txheej txheem PCR zoo ib yam (cov txheej txheem electrophoresis), DNA loop-mediated tas li amplification (LAMP) txoj kev kuaj thiab fluorescent PCR txoj kev. Txoj hauv kev no kov yeej qhov ua tsis tau ntawm txoj kev tshuaj xyuas ib txwm xws li kev ua haujlwm nyuaj, siv sijhawm ntev, thiab nyuaj rau qhov txawv qhov tshwm sim. Nws kuj tseem tau coj mus rau kev txhim kho sai ntawm cov txheej txheem tshawb nrhiav sai rau Enterobacter sakazakii. Txawm li cas los xij, txoj hauv kev no tseem muaj qhov tsis zoo ntawm qhov siab ntawm cov kab mob los ntsuas hauv tus qauv, tus nqi tshuaj xyuas siab, ua haujlwm nyuaj, thiab tsis muaj peev xwm txheeb xyuas tau tias muaj kab mob nyob.