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cov 96 zoo pla yog ib hom ELISA PLATE, yog lub luag haujlwm tseem ceeb hauv Enzyme Linked Immunosorbent Assay (ELISA). Nws koom nrog hauv kev tiv thaiv kab mob tiv thaiv kab mob, xws li: tshuaj tiv thaiv kab mob los yog antigen txheej, tsis thaiv qhov thaiv, thiab lwm yam.
Cov phaj zoo yog tsim hauv chav GMP huv. Cov khoom lag luam tau raug tswj nruj raws li qhov yuav tsum tau ua ntawm ISO thiab ANSI/SLAS Cov Qauv los xyuas kom ruaj khov ntawm cov khoom lag luam. Cov khoom sib txawv sib txawv ntawm txhua lub qhov phaj thiab cov pawg yog tsawg dua 5%. Cov khoom lag luam tau txais kev siv tshuab kho kom zoo dua qub, uas ua tau zoo txhim kho lub peev xwm adsorption ntawm cov protein hauv qhov siab thiab nruab nrab txoj hlua khi ELISA phaj, thiab txhim kho qhov rhiab ntawm cov khoom rau cov protein adsorption kuaj pom. Nws tuaj yeem siv dav hauv ntau hom ELISA, kuaj PCR, Kab lis kev cai ntawm tes, tshawb fawb tshawb fawb, thiab lwm yam.
Raws li tus nyeem ntawv microplate, nws tau muab faib ua qhov tshem tau thiab tsis tshem tawm tau.
Rau qhov tsis tuaj yeem tshem tau, cov kab zoo ntawm txhua lub rooj tsavxwm tau txuas ua ke.
Tom qab ntawd qhov tshem tawm tau yog cov kab txaij ntawm lub rooj sib tham tau sib cais, thiab cov kab sib cais yog 8 lub qhov dej lossis 12 lub qhov dej rau ib kab.
Feem ntau, tshem tawm cov microplates feem ntau siv. Txawm hais tias txhua lub plats zoo muaj qhov zoo ib yam, tab sis cov npe sib txawv muaj lawv tus kheej qhov sib txawv, xws li cov qauv, kev ua kom muaj protein ntau nqus tau, thiab lwm yam. Thaum koj xaiv yuav 96 lub phaj zoo, koj yuav tsum txiav txim siab hom tshuaj twg ntawm microplate nyeem ntawv zoo dua rau koj.
Raws li qhov sib txawv muaj peev xwm ua kom muaj protein ntau thiab lwm yam khoom siv, phaj ELISA tuaj yeem faib ua pawg hauv qab no:
Tom qab kho saum npoo, cov peev txheej khi ntawm cov phaj ELISA tau nce ntau heev, nce mus txog 300-400 ng 1gG/cm2, thiab qhov hnyav ntawm cov protein loj yog> 10kD. Txhawm rau siv hom phaj zoo no tuaj yeem txhim kho qhov rhiab, thiab tuaj yeem txo qis qhov siab thiab ntau npaum ntawm cov txheej txheej. Qhov tsis zoo yog tias nws yooj yim dua los tsim cov kev cuam tshuam tshwj xeeb. Tom qab cov tshuaj tiv thaiv kab mob antigen lossis tshuaj tiv thaiv kab mob, cov tshuaj tsis-ionic tsis tuaj yeem ua haujlwm tau zoo rau qhov chaw tsis muaj cov protein, thiab cov protein yuav tsum tau siv ua lub foob.
Cov phaj ELISA tau dhau los ua ke nrog cov xim dawb los ntawm kev sib koom ua ke hydrophobic rau ntawm qhov chaw, thiab tsim nyog raws li cov khoom thauj khoom ruaj khov rau macromolecular protein nrog lub cev hnyav ntawm 20D. Nws cov peev txheej muaj protein ntau yog 200-300 ng 1gG/cm2. Vim yog tus yam ntxwv ntawm ELISA phaj uas tsuas yog khi rau macromolecules, nws tsim nyog raws li cov khoom thauj khoom rau cov tshuaj tiv thaiv kab mob tsis huv los yog antigens, thiab tuaj yeem txo qhov muaj peev xwm tsis muaj kev cuam tshuam tshwj xeeb. Cov microplate no tuaj yeem siv los ua cov tshuaj tiv thaiv kab mob nrog inert protein lossis tsis-ionic xab npum.
Tom qab hloov kho saum npoo, tus qauv cawv muaj qhov zoo them rau cov pab pawg amino, thiab nws cov hydrophobic daim ntawv cog lus tau hloov los ntawm cov dej-tua nyiaj. Nws yog qhov tsim nyog raws li cov khoom thauj khoom ruaj khov rau cov protein me me. Siv qhov tsis tsim nyog thiab tus nqi pH, saum npoo tuaj yeem khi rau qhov tsis zoo me me them nyiaj los ntawm cov khoom sib txuas ionic. Vim yog lub zog hydrophilic ntawm cov nplaim thiab lub peev xwm los sib cog lus sib cog nrog lwm tus neeg sawv cev sib txuas, nws tuaj yeem siv los ua kom lub cev tsis muaj zog ntawm cov protein molecules yaj hauv cov xab npum xws li Triton-100 thiab Tween20. Nws qhov tsis zoo yog vim qhov txo qis hydrophobicity, qee cov protein molecules tsis tuaj yeem khi; ntxiv rau, nws cov nplaim yuav tsum tau muab ntim kom zoo. Vim yog cov yam ntxwv hydrophilic thiab covalent, cov kua dej sib khi siv yuav tsum muaj peev xwm sib cuam tshuam nrog cov pab pawg tsis muaj tshuaj tiv thaiv kab mob thiab txhua pab pawg ua haujlwm tau zoo hauv cov neeg xaiv tus neeg sib txuas sib txuas.
Kev daws cov protein uas paub zoo tuaj yeem siv rau txheej rau ntawm lub tais zoo rau ELISA. Ntsuas cov protein ntau ntawm cov tshuaj hauv qhov dej tom qab txheej. Tus nqi ntawm cov protein hauv lub qhov dej ua ntej txheej rho tawm cov protein tom qab txheej yog tus nqi ntawm cov protein adsorbed rau ntawm phaj. Ua li no, peb tuaj yeem paub qhov adsorptivity ntawm microplate.
Muaj 4 yam khoom siv: Polystyrene, PVC, Poly-D-Lysine thiab Quartz. Polystyrene feem ntau siv los ua 96 phaj zoo, tab sis polystyrene muaj cov tshuaj tsis zoo ruaj khov thiab tuaj yeem yaj tau los ntawm ntau yam tshuaj kuab tshuaj (xws li cov ntxhiab tsw ntxhiab tsw ntxhiab, cov pa roj carbon halogen, thiab lwm yam), thiab yuav raug corroded los ntawm cov kua qaub muaj zog, alkalis thiab tiv thaiv -qhov roj. Yooj yim hloov xim tom qab raug hluav taws xob los ntawm ultraviolet lub teeb, yog li ceev faj thaum siv.
Qhov tshij: 96 cev
zoo yog qhov feem ntau siv tam sim no, vim tias microplate tau siv nrog microplate nyeem ntawv.
Nqaij tawv: Tshem tau thiab tshem tsis tau (SOLID)
xim: Ntshiab, Dawb, Dub
Paj Rwb:
8 qhov tawg tau zoo thiab 12 qhov tawg tau zoo
Raws li ANSI/SLAS-Cov Qauv 1 txog 4-2004, tus qauv 96 qhov phaj zoo qhov ntev yog 127.76 mm (L) x 85.48 mm (W). Nws yog tus txheej txheem lees paub thoob ntiaj teb, suav nrog 5 tus qauv raws li hauv qab no:
ANSI SLAS 1-2004 (R2012): Qhov ntev hneev taw
hloov kho zaum kawg Lub Ib Hlis 9, 2004
ANSI SLAS 2-2004 (R2012): Qhov siab qhov ntev
hloov kho zaum kawg Lub Ib Hlis 9, 2004
ANSI SLAS 3-2004 (R2012): Hauv Qab Sab Nraud Flange Qhov ntev
hloov kho zaum kawg Lub Ib Hlis 9, 2004
ANSI SLAS 4-2004 (R2012): Txoj Haujlwm Zoo
hloov kho zaum kawg Lub Ib Hlis 9, 2004
ANSI SLAS 6-2012: Zoo Qab Hauv Qab
hloov kho zaum kawg lub Plaub Hlis 09, 2009
1. Cov txheej txheem txheej polystyrene ruaj khov ua tus nqa khoom kuj tseem ua lub luag haujlwm tseem ceeb hauv kev nqus cov antigens, cov tshuaj tiv thaiv kab mob lossis cov tshuaj tiv thaiv kab mob antigen-antibody.
2. Antigens, cov tshuaj tiv thaiv kab mob thiab lwm yam biomolecules tau nqus mus rau saum npoo ntawm cov neeg nqa khoom los ntawm ntau yam txheej txheem, suav nrog kev nqus cov pa los ntawm kev sib txuas hydrophobic, dej/ionic bonds, covalent sib txuas los ntawm kev qhia ntawm lwm pab pawg zoo li amino thiab carbon pawg, thiab los ntawm Hydrophilic tsevneeg tom qab hloov kho saum npoo.
Siv ntau yam kev sim sim: IVD cov tshuaj reagents, Kev Ntsuas Dej Ntws, ELISA, PCR, kev tshawb nrhiav nucleic acid, DNA rho tawm, kab lis kev cai ntawm tes, tshawb fawb tshawb fawb, thiab lwm yam.
Nws tau siv dav hauv cov zaub mov tsis zoo nrog kev xeem ELISA, PCR test thiab Lateral Flow Test. Cov phaj zoo 96 zoo siv hauv tej khoom siv mis hauv cov mis nyuj nyoos nrhiav tshuaj tua kab mob residue. Tsis zoo li cov qauv tsim sab nraud, cov ntawv sau Colloidal Kub yuav tsis tso rau ntawm nitrocellulose daim nyias nyias, nws tau sib cais los ntawm cov sawb, thauj khoom ntawm 96 lub phaj zoo pab kom muaj kev nkag siab zoo, ceev ceev dilute analyte thiab ua kom lub sij hawm tshawb pom.
Cov phaj zoo yog cov khoom siv kuaj sim ntau, tshwj tsis yog 96 zoo pla, muaj qee hom microplates: 6 phaj zoo, 12 phaj zoo, 24 phaj zoo, 48 phaj zoo, 96 phaj zoo, 384 phaj zoo, 1536 phaj zoo.