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Chloramphenicol Test

miv-fsaa-test-kit

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Chloramphenicol

Chloramphenicol xeem yog dab tsi?

Chloramphenicol Test yog ua raws Colloidal Gold Immunochromatography txhawm rau txheeb xyuas Chloramphenicol cov seem hauv zib ntab khoom. Cov khoom siv no tuaj yeem thov rau ntawm qhov chaw nrawm lossis kuaj sim los ntawm ntau lub chaw haujlwm.
Tau Txais Tus Nqi Pub Dawb!

Chloramphenicol yog dab tsi?

1. Txhais

Chloramphenicol yog ib tus neeg sawv cev dav dav rau cov kab mob bacteriostatic. Nws tuaj yeem nthuav tawm mus rau hauv cov kab mob sib kis los ntawm kev ua kom muaj roj ntau, feem ntau ua rau 50s subunit ntawm cov kab mob 70s ribosome, txwv tsis pub transpeptidase, cuam tshuam kev loj hlob ntawm peptide saw, txwv tsis pub tsim peptide saw, yog li tiv thaiv cov protein synthesis. Cov kab mob uas nkag siab zoo lossis nkag siab zoo rau cov khoom no kuj muaj cov kab mob tua kab mob.

2. Chloramphenicol Synthesi

Synthesis of p-nitroacetophenone ua raw khoom, bromination los tsim p-nitro-α-bromoacetophenone, ntsev tsim nrog cyclohexamethylenetetramine, hydrolysis nrog hydrochloric acid kom tau p-nitro-α-aminobenzene Ethyl ketone hydrochloride, acetylated nrog acetic anhydrides nrog formaldehyde, hydroxymethylated muab p-nitro-α-acetylamino-β-hydroxyphenylacetone, txo nrog aluminium isopropoxide kom tau (±) sulose Type-1-p-nitrophenyl-2-acetylaminopropanediol, hydrolyzed tshem acetyl pawg los ntawm hydrochloric acid, thiab nruab nrab nrog alkali kom tau txais (±) sulose type-1-p-nitrophenyl-2-aminopropanediol (cov khoom siv amino), thiab cov txheej txheem crystallization tau siv los daws nws kom tau D (-)-suara qab zib hom amino compound, uas yog thaum kawg tau los ntawm dichloroacetylation.

3. Siv

A. Mob khaub thuas thiab lwm yam Salmonella kis kab mob;

B. Ampicillin-resistant Haemophilus influenzae type B meningitis lossis Streptococcus pneumoniae, Neisseria meningitidis meningitis, rhiab Gram-negative meningitis hauv cov neeg mob ua xua rau penicillin.

C. Lub paj hlwb, tshwj xeeb yog otogenic, feem ntau yog sib xyaw cov kab mob aerobic thiab anaerobic.

D. Cov kab mob anaerobic hnyav;

E. Tsis muaj lwm yam tshuaj tua kab mob uas muaj tshuaj lom tsawg tuaj yeem siv los kho ntau yam mob hnyav uas tshwm sim los ntawm cov kab mob rhiab;

F. Rickettsia tus kab mob tuaj yeem siv rau kev kho mob ntawm Q ua npaws, Rocky Mountain pom kub taub hau, mob qog noj ntshav thiab lwm yam.

4. Chloramphenicol veterinary

A. Chloramphenicol rau dev

Yog tias koj tus dev raug tus kab mob sib kis thiab keratitis, chloramphenicol qhov muag poob yog qhov kev xaiv zoo: nws tus nqi pheej yig, thiab dev tsis muaj kab mob qhov muag tuaj yeem siv nws los yaug lawv ob lub qhov muag thiab ua kom lawv huv.

B. Chloramphenicol rau miv

Paratyphoid kub taub hau, tseem hu ua salmonellosis, yog kab mob sib kis ntawm cov menyuam yaus miv. Feem ntau siv: chloramphenicol subcutaneously lossis intramuscularly, ntau npaum 20 mg ib txhiab lub cev hnyav, ib hnub ib hnub, siv txuas ntxiv rau 3-5 hnub.

Chloramphenicol qhov muag dauv tuaj yeem siv los ntxuav qhov muag. Nws tuaj yeem daws qhov teeb meem ntawm miv kua muag thaum lub qhov muag liab lossis qhov muag nce ntxiv.

5. Dosage

A. Qhov ncauj: 0.5g rau cov neeg laus, ib zaug txhua 6 teev. Thawj koob tshuaj loj tuaj yeem siv rau kev kho mob ua npaws. Tus neeg mob yuav tsum tau thov txuas ntxiv rau 2-3 hnub tom qab lub cev kub cev ib txwm los tiv thaiv kev rov tshwm sim. Qhov siab tshaj plaws tsis ntau tshaj 26g. Cov menyuam yaus ib hnub 25 kg ntawm lub cev hnyav ~ 50 mg, faib tshuaj ib zaug txhua 6 teev. Kev txhaj tshuaj intramuscular lossis tso dej tso dej: 1 hnub ntim: 0.5-1g, faib ua 2 qhov kev txhaj tshuaj. Dilute nrog kua, 1 chloramphenicol (250 mg) nrog tsawg kawg 100ml ntawm diluent.

B. Qhov muag poob: 1-2 tee txhua lub sijhawm, 3-5 zaug hauv ib hnub. Pob ntseg poob: 2-3 tee txhua lub sijhawm, 3 zaug hauv ib hnub. Nco tseg: Chloramphenicol txhaj (muaj ethanol, glycerin lossis propylene glycol thiab lwm yam kuab tshuaj), Nws yuav tsum tau muab rho tawm nrog rab koob qhuav thiab tshee tshee thaum sib tov kom tiv thaiv nag lossis daus ntawm cov nplais.

C. Tom qab cov tsos mob qis dua, qhov ntau npaum li cas yuav tsum raug txo lossis txiav tawm raws li qhov tsim nyog.

Cov phom sij ntawm Chloramphenicol

Qhov tshwm sim tsis zoo tseem ceeb yog txhawm rau txhawm rau ua haujlwm ntawm pob txha hlwb hematopoietic. Muaj ob yam tsos mob:

Ib qho yog thim rov qab txo qis ntawm ntau hom qe ntshav, uas cov granulocytes poob ua ntej. Cov lus teb no cuam tshuam nrog koob tshuaj thiab chav kawm ntawm kev kho. Ib zaug pom, cov tshuaj yuav tsum raug tso tseg hauv lub sijhawm thiab tuaj yeem rov zoo;

Qhov thib ob, tsis tuaj yeem hloov pauv ntshav tsis txaus, txawm hais tias tsawg, muaj cov neeg tuag coob. Cov tshuaj tiv thaiv no yog rau kev ua xua thiab tsis muaj kev sib raug zoo nrog rau chav kawm ntau npaum li cas. Nws tuaj yeem cuam tshuam nrog chloramphenicol inhibiting tib yam 70S ribosome hauv cov kab mob hauv mitochondria ntawm cov pob txha hlwb hematopoietic cells.

Chloramphenicol (levosycin) yog cov tshuaj tiv thaiv kab mob sab nrauv uas muaj zog dua ntawm cov kab mob gram-negative bacilli ntau dua li cov kab mob zoo; nws kuj muaj cov tshuaj tua kab mob rau cov kab mob anaerobic, chlamydia, mycoplasma thiab rickettsiae. Kev nqus ntawm qhov ncauj yog nrawm thiab ua tiav, ntshav protein sib khi tus nqi yog li 60%, ib nrab ntawm lub neej yog 1.5-3.5h, thiab ntim faib yog 0.92L / kg. Nws tuaj yeem nkag mus rau hauv cov kua dej hauv lub cev los ntawm cov ntshav ua rau lub cev tsis muaj zog, tseem tuaj yeem nkag mus rau hauv menyuam hauv plab los ntawm cov ntshav tso ntshav tawm, nws tseem tuaj yeem faib rau mis nyuj, qaub ncaug, hauv siab, ascites thiab kua synovial. 

Qhov tsim nyog tshuaj kho kom zoo tshaj plaws ntau yog 5 ~ 20μg / ml, dhau ntawm qhov no tuaj yeem ua rau muaj kev pheej hmoo ntawm kev txha pob txha pob txha. Qhov koob tshuaj ib txwm rau cov neeg laus yog l ~ 2g / d, noj hauv 3 ~ 4 zaug; los yog tso ntshav tawm tom qab dilution, 1 ~ 2g / d, muab hauv 2 zaug. Cov tshuaj yuav tsum tau hloov kho thaum thov cov tshuaj no thaum lub siab thiab lub raum tsis ua haujlwm.

Nta ntawm Chloramphenicol Test

1. 15 feeb nyeem cov txiaj ntsig

2. Kev txwv tsis pub teb rau EU thiab Asmeskas FDA cov cai

3. Tsis yog cov kws tshaj lij lossis cov cuab yeej xav tau

4. Yooj yim rau kev khiav lag luam thiab nyeem
Chloramphenicol xeem

Kev Tshawb Nrhiav Txoj Kev Chloramphenicol Test

Kev Xeem Chloramphenicol, raws li txoj kev sib tw kub sib xyaw xim, yog siv rau kev sim Chloramphenicol seem hauv cov qauv zib ntab thiab ua tau raws li EU MRL. Cov khoom siv no tuaj yeem thov rau ntawm qhov chaw kuaj los ntawm ntau lub chaw haujlwm

Kuaj ntawm Chloramphenicol Test

Piv txwv muaj xws li: zib ntab

Kev Tshawb Nrhiav Txwv Kev Xeem Chloramphenicol

LOD tuaj yeem hloov pauv raws li qhov xav tau lossis qhov txawv ntawm qhov sib txawv, thov mus saib hauv phau ntawv qhia kom paub meej ntxiv.

Cov Ntaub Ntawv Kev Ua Haujlwm

FluoroquinolonesMRL (ug / kg)
Enrofloxacin0.5
Ciprofloxacin1
Ofloxacin0.5
Flumequine1
Danofloxacin0.5
Norfloxacin0.5
Oxilinic acid1
Lomefloxacin5
Gatifloxacin9
Enoxacin7
Levofloxacin7.5
Danofloxacin5
Pefloxacin5
Sarafloxacin2

Qhov tseem ceeb ntawm Chloramphenicol Test

Chloramphenicol Test tuaj yeem pab cov tuam txhab tsim khoom, cov koom haum tswj hwm, thiab pej xeem saib xyuas cov khoom lag luam zoo thiab pab tib neeg tiv thaiv lawv txoj kev noj qab haus huv.

Cheebtsam ntawm Chloramphenicol Test

1. Cov Khoom Ntsuas: 10pcs

2. Hexane (12ml): 1 lub raj mis

3. Khoom Qhia: 1

4. Micro zoo phaj: 10 npx

5. Re-Hydration Fluid (2ml): 1 lub raj mis

6. Centrifuge Tube (15ml): 20pcs

Cov ntaub ntawv rau Chloramphenicol Test

1. Ethyl Acetate

2. Tov khoom

3. Oscillator

4. Kev Ntsuas Hluav Taws Xob

5. Centrifuge

6. Organomation

7. Mini Pipette

Yuav siv Chloramphenicol Test li cas?

1. Nyeem cov lus qhia thiab cia tus qauv ntawm chav sov (20-25 ℃).

2. Tshem tawm cov ntawv xeem, siv tsis pub dhau 1h.

3. Muab 100µL cov qauv coj mus tso rau hauv lub qhov dej micro, xa dej mus rau thiab nqis rau 6 zaug kom yaj cov tshuaj reagent. Tom qab ntawd, muab tso rau 5 feeb ntawm chav sov.

4. Tso 100µL cov qauv rau hauv cov qauv zoo, sawv ntsug rau 5mins ntawm chav sov.

5. Nyeem qhov tshwm sim tsis pub dhau 5-8mins. Qhov tshwm sim tom qab 5-8 feeb tsis raug.

Kev txhais lus rau Chloramphenicol Test

Kev txhais lus rau kev xeem

Ntsiab lus

Thaum tshuaj tua kab mob tau siv thawj zaug rau tib neeg, peb suav tias lawv yog "elixir". Raws li qhov teeb meem ntawm kev siv tshuaj tua kab mob txuas ntxiv mus zuj zus, lawv qhov kev tiv thaiv kuj tseem ua rau muaj kev puas tsuaj loj rau tib neeg.

Yog li, hauv lub neej txhua hnub, peb yuav tsum zam zaub mov nrog tshuaj tua kab mob seem. Thawj kauj ruam rau kev pab tus kheej: peb yuav tsum kuaj cov tshuaj tua kab mob seem.

Yuav txiav txim li cas Chloramphenicol Test?

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