Betalactam Test Rau Qe

Cov Khoom Siv Ib Leeg, nrawm los txheeb xyuas Betalactam seem hauv cov mis thiab khoom noj siv mis los ntawm kev siv colloidal kub tiv thaiv-chromatography thev naus laus zis. Sai li 7-10 feeb rau cov txiaj ntsig.

Cyclic amides, tsab ntawv Greek siv los qhia tus lej ntawm cov ntiv tes: β-lactam (plaub lub ntsej muag sib txuas).

Cov txheej txheem ntawm kev ua ntawm ntau yam antibiotics-lactam tshuaj tua kab mob zoo ib yam. Lawv tuaj yeem tiv thaiv cell phab ntsa mucin synthetase, penicillin khi cov protein (PBPs), yog li ua rau cuam tshuam ntawm phab ntsa mucin synthesis thiab ua rau cov kab mob cell phab ntsa puas lub cev o thiab lysis. Ib qho ntxiv, qhov ua rau tuag ntawm cov kab mob yuav tsum suav nrog ua rau cov kab mob ua rau lub cev ua haujlwm tsis zoo, thiab cov kab mob hloov pauv yam tsis muaj autolysin pom qhov ua tsis taus.

Lub nplhaib Beta lactam yog ib feem ntawm cov qauv tseem ceeb ntawm ntau tsev neeg tshuaj tua kab mob. Cov tsev neeg tseem ceeb yog penicillin, cephalosporins, carbapenems, thiab monobactams, yog li nws tseem hu ua beta-lactam tshuaj tua kab mob. Yuav luag tag nrho cov tshuaj tua kab mob no ua haujlwm los ntawm kev txwv tsis pub biosynthesis ntawm cov kab mob cell phab ntsa. Qhov no muaj qhov ua rau tuag rau cov kab mob, txawm hais tias ib tus neeg muab cov kab mob feem ntau yuav muaj cov pab pawg me uas tiv taus cov tshuaj tua kab mob beta-lactam. Kev txhim kho cov kab mob tiv thaiv kab mob yog tshwm sim los ntawm ntau cov noob.

Cov tshuaj tua kab mob beta-lactam yog cov tshuaj tua kab mob ntau yam, suav nrog penicillin thiab nws cov txiaj ntsig, cephalosporins, monoamide rings, carbapenems thiab penicillin enzyme inhibitors. antibiotics-lactam tshuaj tua kab mob (β-lactams) hais txog chav kawm tshuaj tua kab mob loj nrog ring-lactam ntiv nplhaib hauv cov qauv tshuaj. 

Yeej, txhua yam tshuaj tua kab mob suav nrog β-lactam nucleus hauv lawv cov qauv txheej txheem yog β-lactams Cov Tshuaj tua kab mob, uas yog siv ntau hom tshuaj tua kab mob uas twb muaj lawm, suav nrog cov penicillins uas siv ntau tshaj thiab cephalosporins hauv tsev kho mob, ntxiv rau cov cephamycins tshiab, thiomycins , monocyclic β-lactams, thiab lwm yam Atypical β-lactam tshuaj tua kab mob. Xws li cov tshuaj tua kab mob muaj qhov zoo ntawm cov tshuaj tua kab mob muaj zog, tsis muaj tshuaj lom, nthuav dav thiab ua tau zoo hauv chaw kho mob.

A. Penicillins
Penicillin kuj tseem hu ua penicillin G. Penicillin yog ib hom tshuaj tua kab mob. Nws hais txog chav kawm ntawm cov tshuaj tua kab mob uas muaj penicillin hauv cov qauv, uas tuaj yeem rhuav tshem cov phab ntsa ntawm cov kab mob thiab ua lub luag haujlwm tua kab mob thaum lub sijhawm rov tsim dua ntawm cov kab mob. Nws yog cov tshuaj tua kab mob rho tawm los ntawm penicillium. Penicillin belongs rau antibiotics-lactam tshuaj tua kab mob (β-lactams).

B. Cephalosporins
Cephalosporin tshuaj tua kab mob tau siv dav siv tshuaj tua kab mob. Cephalosporins yog cov tshuaj tua kab mob ib nrab uas muaj cephalosporins, uas yog antibiotics-lactam cov tshuaj tua kab mob thiab yog cov khoom siv ntawm 7-aminocephalosporanic acid (7-ACA) hauv antibiotics-lactam tshuaj tua kab mob, yog li lawv muaj cov txheej txheem ua kom tsis zoo sib xws.

C. Monoamide nplhaib
Monocyclic beta-lactam tshuaj tua kab mob (monobactams) sawv cev rau tshuaj suav nrog aztreonam thiab carumonan, uas yog cov tshuaj tua kab mob nqaim-spectrum tiv thaiv aerobic gram-negative bacilli. Amidase ruaj khov thiab cuam tshuam rau bacilli tsis zoo suav nrog P. aeruginosa zoo ib yam li ceftazidime. Kev tiv thaiv kab mob zoo thiab cov kab mob anaerobic.

D. Carbapenems
Cov tshuaj tua kab mob Carbapenem yog cov tshuaj tua kab mob β-lactam ntau tshaj plaws nrog rau cov tshuaj tua kab mob dav tshaj plaws thiab cov tshuaj tua kab mob muaj zog tshaj plaws. Vim tias lawv ruaj khov rau β-lactamase thiab muaj tshuaj lom tsawg, lawv tau dhau los ua kev kho mob tseem ceeb tshaj plaws rau kev kis kab mob hnyav Ib qho ntawm cov tshuaj tua kab mob. Nws cov qauv zoo ib yam li lub nplhaib penicillin ntawm penicillins.

E. Penicillinase inhibitors
Thiab qee yam tshuaj tua kab mob, feem ntau yog txhua yam tshuaj tua kab mob uas suav nrog core-lactam core hauv lawv cov qauv txheej txheem molecular yog antibiotics-lactam tshuaj tua kab mob.

Cov tshuaj Beta-lactam suav nrog cov hauv qab no:

A. Penicillins: xws li penicillin G, amoxicillin, ampicillin, thiab piperacillin. Ua ntej siv tshuaj tua kab mob penicillin, koj yuav tsum nug kom ntxaws seb koj puas muaj keeb kwm ntawm kev ua xua ntawm penicillin, lwm yam tshuaj ua xua, thiab kab mob ua xua. Kev kuaj tawv nqaij, qhov ua kom pom tseeb ntawm kev ntsuas tawv nqaij feem ntau yog 500U / ml. Thaum muaj kev poob siab anaphylactic tshwm sim, 0.1% epinephrine 0.5-1ml yuav tsum tau txhaj subcutaneously tam sim ntawd, thiab tsis muaj kev txhim kho hauv kev ua haujlwm kho mob. Rov ua dua nws li 3-5 feeb tom qab, thiab koom tes nrog lwm yam kev ntsuas kev cawm.

B. Cephalosporins: Raws li cov kab mob tua kab mob, ua haujlwm tiv thaiv kab mob, ruaj khov rau β-lactamase thiab lub raum lom, nws tau faib ua plaub tiam. Thawj tiam: cefalexin, cefazolin, cefradine, cefadroxil; tiam thib ob: cefaclor, cefuroxime, cefprozil, cefotiam, thiab lwm yam. Cov uas ua xua rau cephalosporins thiab cov uas muaj penicillin anaphylactic poob siab lossis keeb kwm cov tshuaj tiv thaiv tam sim no raug txwv los ntawm cov tshuaj no.

C. Tshiab β-lactams.

Beta-lactamase yog chav kawm ntawm cov enzymes zais los ntawm cov kab mob tiv thaiv kab mob lactam tshuaj tua kab mob. Nws tuaj yeem ua ke nrog lub nplhaib beta-lactam txhawm rau tshem lub nplhaib beta-lactam thiab raug rhuav tshem, thiab poob cov tshuaj tua kab mob. Nws yog lub laj thawj tseem ceeb rau kev tiv thaiv cov kab mob no.

β-Lactamase inhibitors (β-Lactamase inhibitors) yog chav kawm tshiab ntawm drugs-lactam tshuaj. Plasmid xa khoom tsim tawm β-lactamase, uas ua rau hydrolysis ntawm qee cov tshuaj ring-lactam nplhaib thiab tsis ua haujlwm. Nws yog txoj hauv kev tseem ceeb rau cov kab mob los tawm tsam qee yam tshuaj antibiotics-lactam tshuaj tua kab mob (penicillins thiab cephalosporins).

Kev phiv tshuaj ntawm antibiotics-lactam tshuaj tua kab mob suav nrog: raws plab, kiv taub hau, ua pob khaus, ua xua, sib kis kab mob (suav nrog Candida) (Rossi, 2004). Qee zaum antibiotics-lactam cov tshuaj tua kab mob tuaj yeem ua rau kub cev, ntuav, erythema, tawv nqaij o, angioedema thiab pseudomembranous enteritis.
Cov tshuaj tua kab mob beta-lactam thiab beta-lactamase inhibitors feem ntau mob thiab mob thaum txhaj tib lub sijhawm.

Kwv yees li 10% ntawm cov neeg mob ua xua rau beta-lactam tshuaj tua kab mob. Kev ua xua tshwm sim li ntawm 0.01% ntawm cov neeg mob (Rossi, 2004). Kwv yees li 5-10% ntawm cov neeg mob hla kev nkag mus rau cov tshuaj penicillin, cephalosporins, thiab tshuaj tua kab mob carbapenem.

1. 7 feeb nyeem cov txiaj ntsig

2. Kev txwv tsis pub teb rau EU thiab Asmeskas FDA cov cai

3. Tsis yog cov kws tshaj lij lossis cov cuab yeej xav tau

4. Yooj yim rau kev khiav lag luam thiab nyeem

Betalactam Test yog colloidal immunochromatography kev kuaj pom tias Penicillin G, Ampicillin, Amoxicillin, Oxacillin, Cloxacillin, Dicloxacillin, Nafcillin, Cefquinome, Cefacetrile, Cefalonium, Cefazolin, Cefoperazone, Cepha Ceftiofur hauv qe thiab cov khoom tsim los ntawm qe, thiab ua tau raws li EU MRL. Cov khoom siv no tuaj yeem thov rau ntawm qhov chaw sim nrawm los ntawm ntau lub chaw haujlwm.

Piv txwv muaj xws li: qe thiab cov khoom ua los ntawm qe.

LOD tuaj yeem hloov pauv raws li qhov xav tau lossis qhov txawv ntawm qhov sib txawv, thov mus saib hauv phau ntawv qhia kom paub meej ntxiv.

Betalactam Test tuaj yeem pab cov tuam txhab tsim khoom, cov koom haum tswj hwm, thiab cov pej xeem saib xyuas cov khoom lag luam zoo thiab pab tib neeg tiv thaiv lawv txoj kev noj qab haus huv.

1. Micro-Well Reagent: 8 kuaj/raj, 12 raj/thawv

2. Xeem Sawb: 8pcs/raj, 96pcs/box

3. Phau Ntawv Qhia: 1pc

1. Qauv Raj

2. Pipette & Pipette Ntsis

3. Zoo-Tais

1. Ntxiv 200ul kev sim tshuaj rau hauv lub qhov dej, tom qab ntawd pipet nce thiab nqis rau 10 zaug kom sib xyaw cov qauv nrog cov tshuaj reagent hauv lub qhov dej.

2. Tom qab ntawd muab tso rau 3 feeb.

3. Ntxig qhov ntsuas ntsuas mus rau hauv lub qhov dej zoo nrog cov qauv ntsuas phoo tag nrho.

4. Tom qab 5 feeb, nyeem cov txiaj ntsig.

Cov tshuaj tua kab mob β-lactam sawv cev los ntawm penicillin thiab cephalosporin muaj cov yam ntxwv ntawm kev ua haujlwm tau zoo, muaj kev nyab xeeb siab thiab muaj cov kab mob dav dav. Lawv nyob hauv txoj haujlwm kho mob tseem ceeb hauv kev kho mob. Niaj hnub no, nws tseem yog cov tshuaj tua kab mob xaiv rau G + cocci kab mob.

Cov tshuaj tua kab mob Beta-lactam tuaj yeem siv ua tshuaj kho tsiaj hauv cov nqaij qaib ua teb, uas tuaj yeem ua rau cov beta-lactam seem hauv cov nqaij qaib lossis qe tsim los ntawm nqaij qaib. Beta lactam test rau qe yog qhov tseem ceeb, thiab BALLYA tau kawm tshwj xeeb qhov xwm txheej no thiab tau tsim cov khoom ntsuas nrawm rau tshuaj tua kab mob beta-lactam. Yog lawm, cov neeg xav tau tuaj yeem tshawb pom sai sai thiab yooj yim tshuaj tua kab mob beta-lactam hauv qe. Cov txiaj ntsig ntsuas tau ua raws EU tus qauv. Tsis tsuas yog kev ua haujlwm yog yooj yim, tab sis kuj lub sijhawm luv thiab qhov tseeb yog siab. BALLYA yuav txuas ntxiv ua kev tshawb fawb ntxiv hauv thaj chaw no.

Thov txheeb xyuas phau ntawv teev npe online thiab tiv tauj peb tus sawv cev muag ntawm email: info@ballyabio.com ib lossis sau daim foos tiv toj hauv qab no: