Chloramphenicol Rapid Test (0.2ppb)

cat-fsaa-test-kit

For meat products testing

Specs:25T / box
Testing Time:7-10 mins
Worldwide shipping, free sample
Introduction
The Chloramphenicol Rapid Test (0.2ppb) is based on the Colloidal Gold Immunochromatography to detect the CAP in Aquaculture, poultry or aquatic sediment. This kit can be applied for on-site or lab rapid testing by various departments.

Performance Information
LOD:0.2ppb

Components
Test Kit
20pcs
Micro well
20pcs
Extraction Agent 1 (purified water 30ml)
1bottle
Extraction Agent 2 (Ethyl Acetate 45ml)
2bottles
Extraction Agent 3 (7ml)
1bottle
PE Groves
1packet
Product Instruction
1
Product Reference
 Oscillator
 Mixer
 Electronic Balance
 Centrifuge
 Termovap Sample Concentrator
 Pipette
 Centrifuge Tube(15ml)
 Centrifuge Tube(5ml)


Storage & shelf-life
 At 4~30℃, out of sun light & moisture, 12months

Preprocessing procedure
Refer to the instruction

Test procedure
1. Read the instruction, let the sample and test kit at room temperature(20-30℃).
2. Take out the test card, use it within 1h.
3. Place the card in flat, add 4-5 drop of sample (about 100µL) into the micro well to dissolve the reagent completely. Then, incubate for 4mins at room temperature.
4. Pipette all dissolved sample into the sample well.
5. Read the result within 5-8mins. Other results are invalid.
Interpretation of results
Negative: line T is same or stronger than C
Positive: line T lighter than C
Invalid: line C does not appear 

Results Illustration
Negative:
Positive:
Invalid:
Precautions
Refer to the instruction

If you want to know more, please contact us

    Trivia questions : What is chloramphenicol?

    Chloramphenicol

    Chloramphenicol mechanism of action

    Chloramphenicol is absorbed from the upper part of the intestinal tract, and the drug concentration in the blood can reach a peak value (about 10-13 mg / L) in about 2 hours after 1.0 g oral administration. Plasma t1 / 2 was 2.5 hours on average, and effective plasma concentrations were maintained after 6 to 8 hours. Chloramphenicol is widely distributed in various tissues and body fluids, and the concentration in cerebrospinal fluid is higher than other antibiotics. The dissolution and absorption of chloramphenicol are related to the particle size and crystal form of the preparation. Intramuscular injection has slow absorption and low blood concentration, which is only 50% to 70% of the same oral dose, but has a longer maintenance time. Chloramphenicol for injection is sodium succinate, which has a high solubility in water and hydrolyzes in tissues to produce chloramphenicol.

    Chloramphenicol antibiotic

    Chloramphenicol antibiotics, full English name chloram phenicols, include chloramphenicol, sulfomycin and odorless chloramphenicol.
    Chloramphenicol is a broad-spectrum antibiotic. It is less commonly used because of its greater toxicity to the blood system. Use its eye drops topically to prevent eye infections. Note: ①The main adverse reactions include granulocyte and thrombocytopenia, aplastic anemia, etc. ② Prolonged use can cause optic neuritis, ataxia, and double infection. ③ Sometimes there is a digestive tract reaction. ④ newborns can cause gray baby syndrome, so it is prohibited. ⑤ Mental patients can cause serious reactions, so they are prohibited. ⑥ intramuscular injection is likely to cause serious reactions. The antibacterial spectrum of methanesulfomycin is similar to that of chloramphenicol, and it does not show aplastic anemia. However, when renal function is poor, the dose should be reduced. Children can take odorless chloramphenicol.

    Chloramphenicol uses

    Chloramphenicol tablet

    Chloramphenicol tablets, indications are
    1. Typhoid and other salmonella infections;
    2. Ampicillin-resistant Haemophilus influenzae type B meningitis or Streptococcus pneumoniae, Neisseria meningitidis meningitis, and sensitive Gram-negative meningitis in patients allergic to penicillin;
    3. Brain abscess, especially otogenic, is often a mixed infection of aerobic and anaerobic bacteria;
    4. Severe anaerobic infections, such as those caused by Bacteroides fragile, are especially suitable for those with lesions involving the central nervous system;
    5. No other low-toxic antibacterial drugs can be used to replace various serious infections caused by sensitive bacteria;
    6. Rickettsia infection can be used for the treatment of Q fever, Rocky Mountain spotted fever, and endemic typhus.

    Chloramphenicol ointment

    Chloramphenicol ointment is a topical drug with chloramphenicol as the main ingredient. It is mainly used to treat bacterial infectious skin diseases, such as folliculitis, edema, impetigo, suppurative sweat glanditis, paronychia, cellulitis, etc.; also used to treat secondary bacterial infections caused by other skin diseases, Such as tinea pedis infection, eczema infection, scabies infection, etc.

    Chloramphenicol eye drops

    Chloramphenicol eye drops are eye drops for treating eye diseases. It is mainly used to treat eyes caused by E. coli, Haemophilus influenzae, Klebsiella, Staphylococcus aureus, hemolytic streptococcus and other sensitive bacteria (such as trachoma, conjunctivitis, keratitis and blepharitis) Department of infection.

    Chloramphenicol dosage

    1. Oral: 0.5g for adults, once every 6 hours. A larger first dose can be used to treat typhoid fever. Patients should continue to apply for 2-3 days after normal body temperature to prevent recurrence, the highest dose does not exceed 26g. Children per kilogram body weight 25 ~ 50 mg, divided dose once every 6 hours. Intramuscular injection or intravenous drip: 1 day volume: 0.5-1g, divided into 2 injections. Dilute with liquid. One chloramphenicol (250 mg) should be diluted with at least 100ml.
    2. Eye drops: 1-2 drops each time, 3-5 times daily. Ear drops: 2-3 drops each time, 3 times daily. Note: Chloramphenicol injection (containing solvents such as ethanol, glycerol or propylene glycol), It should be extracted with a dry syringe and shaken while diluting to prevent precipitation of crystals.
    3. After the symptoms have subsided, the dose should be reduced or discontinued as appropriate.

    Chloramphenicol side effects

    The main adverse reaction was the inhibition of bone marrow hematopoietic function. There are two symptoms:
    One is the reversible reduction of various types of blood cells, in which granulocytes first decrease, and this response is related to dose and treatment course. Once found, the drug should be stopped in time and can be resumed;
    The second is irreversible aplastic anemia. Although rare, it has a high mortality rate. This reaction is allergic and has no direct relationship with the dose course. May be related to the inhibition of chloramphenicol in bone marrow hematopoietic cells by the same 70S ribosomes as bacteria.

    Chloramphenicol toxicity

    Hematopoietic system toxicity:
    (1) Dose-related reversible myelosuppression, clinical manifestations of anemia, leukocytes, and thrombocytopenia.
    (2) Irreversible doses are severe and irreversible aplastic anemia, and a few develop into granulocytic leukemia. The majority of patients with aplastic anemia develop after oral chloramphenicol, and the incubation period can be as long as several weeks to several months. They have bleeding tendency, infection and anemia. Some patients with congenital glucose-6-phosphate dehydrogenase deficiency can develop hemolytic anemia.

    Chloramphenicol interactions

    (1) The antibacterial action mechanism of macrolides and lincomycin antibiotics is similar to that of chloramphenicol, which can replace or prevent the combination of chloramphenicol and the 50s subunit of the bacterial ribosome, so the same use can occur Antagonism should not be combined.
    (2) Chloramphenicol is a bacteriostatic agent that inhibits the synthesis of bacterial proteins and interferes with the bactericidal effect of penicillin-type fungicides. The use of both drugs should be avoided.
    (3) Chloramphenicol can antagonize vitamin B6, increase the body's demand for B6, and can also antagonize the hematopoietic effect of vitamin B12.
    (4) Chloramphenicol can inhibit the drug metabolism enzymes of liver microsomes and can affect the efficacy of other drugs, such as significantly extending the anesthesia time of sodium pentobarbital in animals.
    (5) This product is equivalent to certain drugs that inhibit bone marrow, such as colchicine, butapine and penicillamine, which can increase toxicity.

    Summary

    The direct consequence of long-term abuse of antibiotics in aquaculture and animal husbandry is likely to induce antibiotic resistance genes in animals, which will cause potential genetic pollution to the breeding area and its surrounding environment after excretion. Resistance genes are also very likely to spread in the environment, Proliferation poses a threat to public health and food and drinking water safety.
    The Chloramphenicol Rapid Test (0.2ppb) kit provided by BALLYA can effectively detect whether chloramphenicol is contained in aquatic products, further ensuring consumer safety. This kit is easy to operate, and has a short test time and high accuracy.
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