Specs：96T / box Testing Time：7-10 mins Worldwide shipping, free sample
Instrution For BTM1 Rapid Test Kit
Introduction Single Test Kit, rapid to detect a β-lactams, tetracyclines, and aflatoxin M1 residues in milk and dairy product by using colloidal gold immunochromatography technology. As soon as 7-10 minutes to results.
Application For raw milk, whole milk powder, Pasteurized milk.
Spicifications 1. Specificity Not cross-react with macrolides, aminoglycosides, sulfonamides drugs, etc.
2. Limit of Detection Refer to instruction.
Storage & Shelf-Life At 2~8℃ out of sunlight, 12 months
Compolents (96T / box) Instruction 1 Tube 12
Equipments (Option) Timer Incubator Mini Pipette(200μL) Tips for mini pipette Reader
Operations 1. Take 200 μL milk specimen, add into micro well, mix with the pink agent 10 times. Remark: avoid specimen residues too much in the well. 2. Incubate 3 minutes 3. Insert strips into the wells 4. Incubate 5 minutes 5. Take out strips and read results by eye or reader.
Interpretation Visualinspection 1. If C line visible, interpret according to the instruction. T line compare with C line to interpret positive or negative.
2. If C & T are invisible, it’s invalid detection, repeat testing again.
ReaderInterpretation Refer to the instruction
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BTM1 Rapid Test Kit
Principle of BTM1 Rapid Test Kit
Using colloidal gold immunochromatography, rapid detection of β-lactams, aflatoxin M1, and tetracycline residues in milk samples, using the band elimination method to interpret the results, the entire detection process takes only 8 minutes.
Scope of BTM1 Rapid Test Kit
It is used to screen for the residues of β-lactams, aflatoxin M1, and tetracycline in raw cow milk, high-temperature sterilized milk and milk powder.
β-lactam antibiotics refer to a large class of antibiotics with a β-lactam ring in the chemical structure, including the most commonly used penicillins and cephalosporins in the clinic, as well as the newly developed cephamycins, thiomycins, monocyclic β-lactam and other atypical β-lactam antibiotics. Such antibiotics have the advantages of strong bactericidal activity, low toxicity, wide indications and good clinical efficacy. The chemical structure of this class of drugs, especially the changes in side chains, have resulted in many antibiotics with different antibacterial spectrum and antibacterial effects and various clinical pharmacological properties.
β-lactam mechanism of action
The action mechanisms of all β-lactam antibiotics are similar and can inhibit cell wall mucopeptide synthase.
β-lactam side effects
Side effects of β-lactam antibiotics include: diarrhea, dizziness, rash, urticaria, overlapping infections (including Candida) (Rossi, 2004). Occasionally, β-lactam antibiotics can cause fever, vomiting, erythema, and skin Inflammation, angioedema, and pseudomembranous enteritis (Rossi, 2004) When β-lactam antibiotics and β-lactamase inhibitors are used at the same time, the injection site often causes pain and inflammation.
Tetracycline is the most basic compound in the tetracycline family of antibiotics. It and its salts are yellow or light yellow crystals. It is extremely stable in the dry state. Except for chlortetracycline, the aqueous solutions of other tetracycline families are quite stable. The tetracycline family is soluble in dilute acids, dilute alkalis, etc., slightly soluble in water and lower alcohols, but insoluble in ethers and petroleum ethers. Tetracycline antibiotics mainly include chlortetracycline, oxytetracycline, and tetracycline. Tetracycline antibiotics have a common chemical structure. Both chlortetracycline and oxytetracycline are derivatives of tetracycline. The former is chloretracyelin, the latter is oxyeracyein, and the tetracycline family are both acid-base amphoteric compounds.
Tetracycline mechanism of action
The mechanism of antibacterial action of many antibiotics is to interfere with the process of cell division within or outside the bacteria. Tetracycline antibiotics form a reversible conjugate with the 30S subunit of the bacterial inner ribosome, inhibit protein synthesis, and play an antibacterial effect. When the antibiotic concentration is low, this reversible competitive binding will also be useless, and bacterial protein synthesis will continue. Tetracycline can also inhibit mitochondrial protein synthesis by binding to the mitochondrial 70S subunit. The binding ability of tetracycline to the 80S subunit of eukaryotic cells is relatively weak, so the ability to inhibit protein synthesis in eukaryotic cells is also weak.
Tetracycline hydrochloride is a broad-spectrum antibiotic, which has an inhibitory effect on most Gram-positive and negative bacteria, high-concentration bactericidal effect, and can inhibit Rickettsia, trachomavirus, etc. It has a good effect on Gram-negative bacteria. Its mechanism of action is mainly to prevent the binding of aminoacyl groups to ribonucleosomes, to prevent the growth of peptide chains and protein synthesis, thereby inhibiting the growth of bacteria, and also has a bactericidal effect at high concentrations.
Aflatoxin is a relatively strong carcinogen, aflatoxin m1, which is also a structurally similar compound belonging to aflatoxin. This aflatoxin m1 may be produced in some animal feeds or in foods in humid areas, which is also classified as a carcinogen of Class 2b by the World Health Organization.
Production of aflatoxin m1
The toxoid is a metabolic product produced by the common Asperillus Flavus and Asperillus Parasiticus. Among them, aflatoxin B1 is the most important toxin. Mammals consume feed contaminated with AFB1 or after food, catalyzed by the liver microsome monooxygenase system in the body, and regulated by cytochrome P-448, the AFB1 terminal furan ring C-10 is hydroxylated to produce AFM1. AFM1 can also be directly produced by some Aspergillus flavus and Aspergillus parasite, but the ratio is quite low compared to other toxins (such as B1, B2, G1, G2).
Hazards of aflatoxin m1
The toxicity of AFM1 is mainly manifested in carcinogenicity and mutagenicity. Studies on the physiological carcinogenic mechanism show that: the distal furan ring epoxy structure of AFM1 covalently binds with DNA purine residues in the body, causing some damage to DNA, causing DNA structure and The functional change, which produces cancer, is basically similar to the carcinogenicity of AFB1, but the toxicity is lower than AFB1. However, compared with potassium cyanide and arsenic, it is still a particularly highly toxic substance and a strong carcinogen.
Beta-lactam antibiotics, tetracycline and aflatoxin M1 are widely used antibiotics in various industries. The side effects include severe allergies, etc. If people inadvertently ingest too much, it will have adverse effects. Therefore, it is very important to detect β-lactam antibiotics, tetracycline and aflatoxin M1 in milk.