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Nitrite Rapid Test For Milk

Trimethoprim-Test-Kit

For Milk, Milk Powder, Pasteurized Milk

Specs:96T / box
Testing Time:7-10 mins
Worldwide shipping, free sample

Operation video

Instrution For Nitrite Rapid Test For Milk

INTRODUCTION
The Nitrite rapid test kit is based on the Colloidal gold immunochromatography to detect raw, commingled cow milk at or below established tolerance and/or safe levels. This kit can be applied for on-site rapid testing by various departments.

Kit Components
1. Test Strip, 12 packs, each pack 8 strips.
2. Manual Instruction

Components Required, But Not Provided
1. Sample Tube
2. Pipette
3. Well-tray

Storage
Storage at 2~8℃, out of sun light and moisture, unrefrigerated.

Milk Sample Information
1. Raw, commingled cow milk
2. The milk has no precipitation or clotted.
3. The temperature of milk should be at room temperature, No need to heat or refrigerate.
4. Thoroughly mix with the reagent before testing.

Test Procedure
1. Read the manual instruction before testing. Be sure the raw or commingled milk and test kit in room temperature. (Note: the milk must has no precipitation or clot)
2. Open the pack, take out the test strip and sample-well, use it within 1 hour. The unused test strip must be sealed and out of sunlight and moisture.
3. Take the sample milk, let it become room temperature. Drop 200ul into the microwell, repeatedly pipetting up and down for 10 times, mix the with the reagent in the wells completely.
4. Then wait for 5mins.
5. Insert the test strip into the microwell with the end fully dipped.
6. Timer 5 minutes, then read the results.

Results Illustration

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Trivia questions : What is Nitrite?

Nitrite definition

Nitrite

Nitrite, a general term for a class of inorganic compounds. Mainly refers to sodium nitrite, which is white to light yellow powder or granular, slightly salty, and easily soluble in water.

Nitrite lewis structure

Nitric acid is a kind of strong oxidizing and corrosive strong acid. It belongs to monobasic inorganic strong acid. In industry, it can be used to make chemical fertilizers, pesticides, explosives, dyes, salts and so on.

The Lewis structure, also known as the Lewis point diagram, is a drawing that is used to express the chemical bonds between atoms in a molecule and each possible lone electron pair.

Nitrite and nitrate

Nitrite and nitrate are interchangeable. Nitrate is converted into nitrite under oxidizing environment. Pure water does not contain other chemical impurities. General drinking water and food contain nitrite and nitrate. Since nitrite is a toxic substance (of course, it has also been used as a preservative), and the nitrite content in food must be calculated before it can be sold. When calculating the nitrite content, sometimes nitrate It is also oxidized to calculate nitrite. If food safety is considered, nitrate is actually a substitute for nitrite. Under high temperature cooking or long-term aerobic environment, nitrate will be converted into nitrite.

Nitrite source

In fact, the source of nitrite in food is nitrate. Excessive nitrate can reduce nitrate to nitrite under the action of a series of bacteria's nitroreductase. Nitrate in human body mainly comes from food and drinking water, and the content of nitrate in groundwater is one of the safety standards for drinking water. Sources of nitrite that enter the body include:
1. During the cultivation of vegetables and foods, excessive application of nitrogen fertilizers causes excessive nitrate accumulation in foods. If the vegetables are stored in poor conditions and for a long time, bacteria will multiply and the vegetables will begin to rot. At this time, the nitrite content will significantly increase.
2. Nitrite content will increase after cooked vegetables are stored for a long time.
3. In order to maintain the bright red color of some meat products during processing to improve the ornamental and flavor, nitrate and nitrite are used as colorants.
4. According to research, after the wild vegetables are canned and canned, the nitrate content is reduced, but the nitrite content is increased.
5. In vegetables, the content of nitrate is generally: the content of root vegetables is greater than leaf vegetables, and leaf vegetables are greater than eggplant fruits.
6. Drink bitter well water containing nitrate or nitrite.

Nitrite in milk

The standard for allowing nitrite in milk is 0.2 mg / kg. Under normal circumstances, because the rumen microorganisms of the cattle have the function of decomposition and utilization, so nitrite does not appear to exceed the standard in milk. The reasons for exceeding the standard may be that raw milk has been stored for a long time, and the feed and water eaten by cows contain nitrite. In addition, it is mainly due to the surface water contaminated by groundwater and a variety of agricultural and non-agricultural activities. , Such as the application of fertilizers, the decomposition of animal manure, nitrogen residues of crop residues, certain minerals, septic tanks, municipal waste, etc., which increase the content of nitrate and other substances in groundwater, once the amount exceeds the plant's ability to use, It can accumulate in the lower layer of soil or penetrate into the groundwater. Under the action of certain microorganisms in the environment, it will be converted into nitrite.

China mainly occurs in coastal sandy soils and low water wells. Therefore, the prevention of water wells is the first choice. The wells should not be too shallow. There should be well fences to keep away from manure pollution and prevent surface water from flowing into the wells. It is best not to use well water for dairy cows to drink and wash milk, and use tap water instead. Pay attention to environmental hygiene and reduce nitrate degradation caused by bacterial contamination. Some green materials will produce nitrite after they are piled up and heated, so the green materials purchased should be spread out in time to prevent the pile from heating up and deteriorating.

Nitrite poisoning

Nitrite poisoning is caused by eating marinated meat products, pickles and spoiled vegetables with high nitrate or nitrite content, which can cause poisoning, or caused by the accidental consumption of industrial sodium nitrite as table salt. It can also be seen in drinking Containing nitrate or nitrite in bitter well water and steamer water, nitrite can oxidize methemoglobin, which normally carries oxygen in the blood, to methemoglobin, thus losing the ability to carry oxygen and causing tissue hypoxia. Nitrite is a highly toxic substance. Adults ingesting 0.2 to 0.5 grams can cause poisoning, and 3 grams can cause death. At the same time, esophageal cancer has a positive correlation with the amount of nitrite ingested by the patient. The carcinogenic mechanism of nitrite is: under the environment of gastric acid and other environments, nitrite reacts with secondary amines, tertiary amines and amides in food to form a strong carcinogen N. A nitrosamine. Nitrosamines can also enter the fetus through the placenta and have teratogenic effects on the fetus.

Nitrite test

Nitrite test in urine

The urine nitrite test (NIT), also known as the Griess test, is a screening test for urinary tract infections. The diagnosis of urinary tract infection requires urinary bacterial culture, which requires a long time and certain conditions. A qualitative test using urinary nitrite can quickly get a result to screen for urinary tract infections to help analyze whether there are problems with urinary tract infections and bacteriuria. When the urinary tract infection bacteria have the ability to reduce nitrate to nitrite, the urine nitrite test is positive.

Nitrite test kit

Detection principle: Nitrite reacts with the reagent on the test paper, and the content of nitrite in food can be detected according to the color change of the test paper.

Significance of detection: Nitrite poisoning can cause dyspnea or even death due to circulatory failure. It can also form strong carcinogen nitrosamines with amines under suitable conditions, so the detection of nitrite has important significance.

Summary

Nitrite is a food additive commonly used in various countries in the world, and the main representative substance is sodium nitrite. Because it can be combined with myoglobin in meat to form nitrosyl myoglobin, which maintains a good appearance, has strong reducibility, and has a certain preservative effect, nitrite is often used as a color protectant and preservative.
Due to methemoglobinemia caused by acute nitrite poisoning, chronic poisoning has teratogenic and carcinogenic risks. Therefore, the amount of addition must be strictly controlled.
BALLYA Milk Nitrite Test Kit can quickly and effectively detect whether the content of nitrite in milk exceeds the standard. The test results meet EU standards, with short test time, simple operation, and can be tested anytime, anywhere.
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