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Aflatoxin B1 Rapid Test kit (R&D)

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cat-fsaa-test-kit

For Agricultural Products

Specs:10 T / box
Testing Time:15 mins
Worldwide shipping, free sample
Introduction
The Aflatoxin B1 Rapid Test kit is based on the Colloidal Gold Immunochromatography to detect the Amantadine residues in aquaculture products. This kit can be applied for on-site or lab rapid testing by various departments.

Components
Test card
20pcs
Extraction Agent A -Ethyl Acetate (40ml)
3bottles
Extraction Agent B (22ml)
1bottle
PE Groves
1packet
Micro well
20pcs
Re-Hydration Fluid(7ml)
1bottle
Centrifuge Tube (15ml)
20pcs
Product Instruction
1
Storage & shelf-life
At 4~30℃, out of sun light & moisture, 12 months

Performance Information
Fluoroquinolones
MRL (ug/kg)
Enrofloxacin
3bottles
Ciprofloxacin
1bottle
Norfloxacin
1packet
Ofloxacin
20pcs
Flumequine
1bottle
Danofloxacin
20pcs
Norfloxacin
1
Oxilinic acid
1
Lomefloxacin
1
Gatifloxacin
1
Enoxacin
1
Levofloxacin
1
Danofloxacin
1
Pefloxacin
1
Sarafloxacin
1
Product reference
Centrifuge Tube (15ml) 40pcs

Preprocessing procedure
Refer to the instruction

Test procedure
1. Read the instruction and let the sample at room temperature (20-25℃).
2. Take out the test strip, use within 1h.
3. Drop 100µL of sample into the micro well, pipetting up and down for 6 times to dissolve the reagent. Then, incubate for 5mins at room temperature.
4. Drop 100µL sample into the sample well, incubate for 5mins at room temperature.
5. Read the result within 5-8mins. The result after 10mins is invalid.
Interpretation of results
Negative: line T is same or stronger than line C
Positive: only line C appear or line T is hardly appearing
Invalid: line C does not appear 

Results illustration
Negative:
Positive
Invalid:
Precautions
Refer to the instruction

If you want to know more, please contact us

Aflatoxin B1 Rapid Test kit

Aflatoxin B1 Rapid Test kit measurement principle

The aflatoxin test kit uses indirect competitive ELISA to detect aflatoxin B1 in urine and tissue samples. The microwell strips are pre-coated with the coupled antigen, using the principle of specific immunochemical reaction between the antigen and antibody to carry out, the aflatoxin B1 in the sample and the anti-aflatoxin B1 antibody pre-coated with the antigen conjugated with horseradish peroxidase on the microwell strip are pre-coated with the TMB substrate after washing. The content of aflatoxin B1 is inversely proportional to the absorbance value of the sample, and the content of aflatoxin B1 can be obtained by comparing with the standard curve.

Detection Limit of Aflatoxin B1 Rapid Test kit

0.1ppd, this detection limit may change, please refer to the kit instructions.

Precautions for Aflatoxin B1 Rapid Test kit

1. Please read the instructions carefully before using the kit.
2. Do not use expired kits.
3. Before using the kit, return the reagent to room temperature (25 ± 2 ℃). It is recommended to warm it up for at least 2 hours.
5. The termination fluid contains sulfuric acid, which can prevent skin burn and corrosion of clothing when used.
6. The tips used for different standards and samples can not be mixed, otherwise it will affect the test results.
7. The reagents in different batch kits should not be mixed; the tips used for different standards and samples should not be mixed, otherwise it will affect the experimental results.
8. The sample dilution in this kit must be used when diluting the sample, otherwise it will affect the experimental results
9. Avoid foaming when mixing reagents.

The aflatoxin testing kits' operation steps

1. Take the required reagents out of the 4 ℃ refrigerated environment, put them at room temperature (20-25 ℃) and equilibrate for more than 30min. Note that each liquid reagent must be shaken before use.
2. Take out the required number of microplates and frames, put the unused microplates in a ziplock bag, store at 2-8 °C and do not freeze.
3. Numbering: Number the microwells corresponding to the samples and the standard products in sequence. Each sample and the standard product are paralleled with 2 wells, and record the positions of the standard wells and the sample wells.
4. Add 50μl / well of standard / sample to each microwell, then add 50μl / well of enzyme marker, and then add 50μl / well of antibody working solution. Seal the plate with a cover film, gently shake and mix, and react for 30 min in a 25 °C environment.
5. Carefully peel off the cover film, dry the liquid in the well, wash it thoroughly with washing solution 250μl / well 4-5 times, each time 15-30 seconds, pat dry with absorbent paper Bubbles can be pierced with a clean pipette tip.
6. Color development: Add 50μl of Substrate A solution to each well, and then add 50μl of Substrate B solution, and gently shake to mix at 25 °C for 15min in the dark.
7. Determination: Add 50μl of stop solution to each well, gently shake and mix, set the microplate reader at 450 nm (recommended to use dual wavelength 450 / 630 nm, test and read the data within 5min) to measure the OD value of each well.

About Aflatoxin B1

Aflatoxin B1 is present in the soil, various nuts in animals and plants, especially peanuts and walnuts. Aflatoxins are also often found in soybeans, rice, corn, macaroni, condiments, milk, dairy products, edible oils and other products. Generally, tropical and subtropical regions such as southern high-temperature and high-humidity areas are most contaminated, and the detection rate of aflatoxins in food is relatively high. Aflatoxin is heat-resistant and can only be cleaved at 280 ℃, so it is difficult to destroy at ordinary cooking and processing temperatures.

Aflatoxin B1 toxicity

Aflatoxin B1 is 30 times more toxic than vomitotoxin and 20 times more zearalenone. The acute toxicity of aflatoxin B1 is 10 times that of potassium cyanide and 68 times that of arsenic. Chronic toxicity can induce canceration. The carcinogenic capacity is 75 times that of dimethylnitrosamine, which is 900 times higher than that of dimethylazobenzene. Human primary liver cancer may also be related to aflatoxin.

Aflatoxin B1 metabolism and distribution

Aspergillus B1 metabolism mainly occurs in the liver, and the kidney, spleen, and adrenal glands are also distributed, and generally do not exist in muscles. If aflatoxin is not taken continuously, it generally does not accumulate in the body. Most of it is excreted by breathing, urine, feces, etc. about 1 week after one ingestion. The parent compound of aflatoxin B1 without metabolic activation is non-carcinogenic, so it is called a pre-carcinogen because it must form an active intermediate through biological transformation in the body, that is, "metabolic activation or biological activation" to be carcinogenic.

Summary

Since aflatoxin B1 is very toxic to humans, the Chinese food hygiene standards stipulate the allowable amount of aflatoxin B1 in several main susceptible foods, and aflatoxin B1 in corn, peanuts and peanut oil is allowed The amount is ≤20μg / kg. The EU and other countries revised the provisions on the content of AFTB1 in grains, peanuts and their products in 2002. The content of AFTB1 in peanuts used directly by humans should be ≤2μg / kg, and the content of peanuts imported as food raw materials should be ≤8μg / kg.
Food testing is the best prevention against aflatoxin B1. Aflatoxin B1 Rapid Test kit will be your best choice.
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